Status
Please wait ...
Gene information
General information
TC011887 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12224517..12226732
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011887
    iBeetle screen iB_10549

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011887 RA
      MDEFVKLTW RKEVTDPDL DERFLSEEA VSESYVYKS 
      FTKPIKSKP KRNVKLHVA KEKYVPSLK YHRLIRRRS 
      LLTKSQKSY CLEALKLIQ SGKRSDLLT EREKCCLKI 
      YGECQSKIV EENQLFQEY AKNMWLKYK DKPLKTKPG 
      IYMYAWPKW KQKLCRYLD YPQFYKEIA SVCLNYDEN 
      EATVDFQHL VQPQETGTL ARFVQPNLK EQCCLKMNT 
      FQKVAKEVL LNSKLPVSQ DDNIPKLLK SHKFDAVIS 
      SSGLKQIID YTNIKTKWI IPVVIKEIE DEGGKLRKV 
      VFIDKVLPK VDPSPHDLN CYAYKRLLK LSFCEYEAF 
      KYPVEETPI GEGDEQDCE TEDPKDRKK VIHHNANYR 
      IWNIKKTTS HNTLMKNQS KDTEINLLV RCKLDGCEE 
      TEQSVLYPV TVRPKIEQQ MQYGVNIAS RSEIARDWT 
      SLFFRLYSF LYRARIYSP MHEVISVEK CSIQKINIE 
      AQTHYDYKP ILGLGVLEK VLSQLVGLE CGTYLLQHL 
      PKHESFLSI MKQSDCTEN AYDLHKECE ETKVNTEGR 
      LSWLPIDVN YILPVHEEY KQIPAVFHG FDNKKEPSG 
      TTVSDQFA
    CDS sequence
    • >TC011887 RA
      atggatgaa ttcgtgaaa ttaacttgg cgcaaagag 
      gtgactgat ccggatctg gacgagcga tttttgagc 
      gaggaggca gtgtcagag tcctacgtt tacaagagt 
      tttactaaa ccaattaaa tccaaaccg aaacgaaac 
      gttaagcta catgtagct aaagaaaaa tacgtccct 
      agtttaaag tatcaccgc ttaattcgg cgcagatct 
      ctactaaca aagtcacaa aagtcgtac tgtttggag 
      gccctaaag ttaatccag agtggcaaa aggtccgac 
      ctattgacc gaaagagag aagtgctgt ctcaaaatt 
      tatggtgaa tgccagtca aagattgtt gaggaaaat 
      caattattt caagaatat gccaaaaat atgtggctc 
      aaatacaag gacaagcct ttgaagacg aaaccgggg 
      atttacatg tacgcttgg ccaaaatgg aagcagaaa 
      ctctgcaga tatttggac tatccccaa ttttacaag 
      gaaattgct tcggtttgt ttaaactac gacgagaat 
      gaggccact gtcgatttt caacatttg gtgcaacct 
      caggaaacg ggcacactg gcccgcttt gtgcaacca 
      aacctcaag gaacagtgc tgtttaaaa atgaacacg 
      ttccaaaaa gtggcaaag gaggttttg ctgaactca 
      aaattgcca gtcagtcaa gacgataat ataccaaaa 
      ctgctcaaa agccacaaa tttgacgct gtgatttcg 
      tccagtggc cttaagcaa attattgac tacactaat 
      attaaaact aagtggatc atcccggtt gtgattaaa 
      gaaattgaa gacgagggt ggcaaactt cgtaaagtt 
      gttttcatt gataaagtg ctaccaaaa gttgacccc 
      agtccgcac gatttaaac tgttatgcc tacaaacgg 
      ctcctaaaa ttgagtttt tgcgaatat gaggcgttt 
      aagtatcct gttgaggag acaccaatt ggtgaagga 
      gacgaacaa gactgcgag accgaagac ccaaaagat 
      aggaaaaaa gtcattcat cataacgcg aattaccgc 
      atttggaat ataaaaaag acaacaagt cacaatact 
      ctaatgaaa aatcagagc aaagacacc gaaattaac 
      cttttggtt cgctgcaaa ttggacgga tgcgaagaa 
      actgagcaa agtgtgtta tatccggtc actgtgcgg 
      cctaaaata gagcaacag atgcagtac ggggtcaat 
      atcgcgtcg cggagcgaa atcgctcgt gactggacc 
      tcgcttttc tttcgtctt tattcgttt ctttacaga 
      gcgcgcatt tatagcccc atgcacgaa gttatctcg 
      gtcgagaaa tgctcaata caaaagatc aacatcgag 
      gcacaaact cactacgat tataaaccc attctaggt 
      ttaggtgtt ttggaaaag gtcttgagc cagttggtg 
      ggtctcgag tgtggcact tatcttctg caacattta 
      cccaaacat gagtcgttt ctttcaatc atgaaacaa 
      tccgattgc actgaaaat gcgtacgat ttgcacaaa 
      gaatgcgag gaaactaaa gtaaatacg gaagggcgg 
      ctaagttgg ttacctatt gatgttaat tatattttg 
      cccgtgcat gaggagtat aaacaaatt cctgcggtt 
      tttcacggt tttgataac aaaaaggag ccaagtggt 
      acgacggta agtgatcaa tttgcttaa
    mRNA sequence
    • >TC011887 RA
      caacgaccg taggtattt tattgtaaa tataacctt 
      cattatcac attttgaac gagatggat gaattcgtg 
      aaattaact tggcgcaaa gaggtgact gatccggat 
      ctggacgag cgatttttg agcgaggag gcagtgtca 
      gagtcctac gtttacaag agttttact aaaccaatt 
      aaatccaaa ccgaaacga aacgttaag ctacatgta 
      gctaaagaa aaatacgtc cctagttta aagtatcac 
      cgcttaatt cggcgcaga tctctacta acaaagtca 
      caaaagtcg tactgtttg gaggcccta aagttaatc 
      cagagtggc aaaaggtcc gacctattg accgaaaga 
      gagaagtgc tgtctcaaa atttatggt gaatgccag 
      tcaaagatt gttgaggaa aatcaatta tttcaagaa 
      tatgccaaa aatatgtgg ctcaaatac aaggacaag 
      cctttgaag acgaaaccg gggatttac atgtacgct 
      tggccaaaa tggaagcag aaactctgc agatatttg 
      gactatccc caattttac aaggaaatt gcttcggtt 
      tgtttaaac tacgacgag aatgaggcc actgtcgat 
      tttcaacat ttggtgcaa cctcaggaa acgggcaca 
      ctggcccgc tttgtgcaa ccaaacctc aaggaacag 
      tgctgttta aaaatgaac acgttccaa aaagtggca 
      aaggaggtt ttgctgaac tcaaaattg ccagtcagt 
      caagacgat aatatacca aaactgctc aaaagccac 
      aaatttgac gctgtgatt tcgtccagt ggccttaag 
      caaattatt gactacact aatattaaa actaagtgg 
      atcatcccg gttgtgatt aaagaaatt gaagacgag 
      ggtggcaaa cttcgtaaa gttgttttc attgataaa 
      gtgctacca aaagttgac cccagtccg cacgattta 
      aactgttat gcctacaaa cggctccta aaattgagt 
      ttttgcgaa tatgaggcg tttaagtat cctgttgag 
      gagacacca attggtgaa ggagacgaa caagactgc 
      gagaccgaa gacccaaaa gataggaaa aaagtcatt 
      catcataac gcgaattac cgcatttgg aatataaaa 
      aagacaaca agtcacaat actctaatg aaaaatcag 
      agcaaagac accgaaatt aaccttttg gttcgctgc 
      aaattggac ggatgcgaa gaaactgag caaagtgtg 
      ttatatccg gtcactgtg cggcctaaa atagagcaa 
      cagatgcag tacggggtc aatatcgcg tcgcggagc 
      gaaatcgct cgtgactgg acctcgctt ttctttcgt 
      ctttattcg tttctttac agagcgcgc atttatagc 
      cccatgcac gaagttatc tcggtcgag aaatgctca 
      atacaaaag atcaacatc gaggcacaa actcactac 
      gattataaa cccattcta ggtttaggt gttttggaa 
      aaggtcttg agccagttg gtgggtctc gagtgtggc 
      acttatctt ctgcaacat ttacccaaa catgagtcg 
      tttctttca atcatgaaa caatccgat tgcactgaa 
      aatgcgtac gatttgcac aaagaatgc gaggaaact 
      aaagtaaat acggaaggg cggctaagt tggttacct 
      attgatgtt aattatatt ttgcccgtg catgaggag 
      tataaacaa attcctgcg gtttttcac ggttttgat 
      aacaaaaag gagccaagt ggtacgacg gtaagtgat 
      caatttgct taaaacctt ttcattcga acgacagcg 
      aactaaata caagataca aaatgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY