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Gene information
General information
TC005297 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450983.1: 490..3513
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005297
      iBeetle screen iB_10548

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005297 RA
        MTLKILQAN VGRAYAVQD LVCATANQL KADLLVISE 
        PNKKRIEKS NWIKDKMRD VAMLVLTKN VGIVSHTIF 
        DGYLMIQTK KLNIIGCYI SPNIGIQDY QKKVDSIMK 
        EVKYGETIV LGEFNGKSP LWGSPKKDE KGDYWVQWI 
        SARDMVVLN TGLRPTFVR GESESYIDV TMATNKIAG 
        DVTNWEILD IETLTEHKY IYFEIRTNS GKIIRDVGN 
        KRIVAKYAN TLRPYELDI EKKKQVVTD LFPTEKEEW 
        AIFQIDDKP TQFTLEELR TAAVGIKCG KAPGVDKIP 
        PDAVKIVAT CAPNWLLSI MNGLLQVQN FPDEWKIAK 
        IVLILKPGK PTEIAASSY RPLCMLNSL SKLLETLIK 
        NRLQSELVN RGGLHPRQY GFQKGKSTI QAVEKALAI 
        ARDFNRKYC TLITIDVKN AFNSASHKI IIEKLQEKR 
        IPKYLINFI SSYLKNRKI MIKPGSMIK VTAGVPQGL 
        VLGPTLWNI LYDEILSIE LTSDAKTIG FADDLTLIV 
        GASEDKTFV NNTNECLAR INKWMKSHK LQLAPNKTE 
        AVLLRGMQK RERIMFTIE GVNITPSKT LKYLGIIID 
        ERLTFGSHV ETAILKAEK NISTLTRIL PNIGGPRDS 
        KRKVLCSAV QNIVLYGAP VWEKALETK KQYTAIHKL 
        QRKMLLRVA SAFKTVSGL ALQTVTGIL PIDIMVQER 
        SYMNQHRLE DKKQTEISA RERSLKQWE NRWMRETNK 
        TQWTKRLIP NLRIWLNCK HRQTDYYLT EVLTEHGVL 
        RSYAKRFAK DTVEECIFC SRNDTVEHT IFECEKWNN 
        IRESTYNQL GCTLEPGNL VQKMIDNKT NWDTIYNMI 
        KHIMKRKEK AEINRQDEM KSR
      CDS sequence
      • >TC005297 RA
        atgactcta aaaatacta caagccaac gttggacga 
        gcatatgca gttcaagat ctagtctgt gccacagca 
        aaccagcta aaagctgat ttgctagtt atcagcgaa 
        ccaaataaa aaaagaata gaaaaatca aactggatt 
        aaagacaaa atgagagac gtggcaatg ttagtccta 
        acaaagaat gttggtatt gtaagtcat actattttt 
        gacggttac ttaatgatc caaacaaaa aaacttaat 
        ataattgga tgctatata tcacccaat ataggaata 
        caagattac caaaaaaag gtagatagc ataatgaag 
        gaagttaaa tatggagaa accatagta ttgggagag 
        tttaacggc aaatctccg ctatgggga tcacctaaa 
        aaagatgaa aagggcgac tactgggtg cagtggatc 
        tcggcgcgc gacatggtt gttctgaat actggacta 
        agacctacc tttgtacgg ggagaaagc gaatcatat 
        atcgacgta acaatggcc actaataaa atcgcagga 
        gatgtaact aattgggag attctggac atcgagaca 
        ctgacagag cataaatac atctacttt gaaattagg 
        acaaattct ggaaaaata ataagggat gtgggcaac 
        aaacgaata gtagcaaaa tatgcaaat accttaaga 
        ccatacgaa ttagatatt gaaaagaaa aagcaggta 
        gtaacagac cttttccca acagaaaag gaagagtgg 
        gcgatcttt caaatagat gacaaacca acacaattc 
        acactagag gagctgaga actgcagcg gttggcata 
        aaatgcggg aaggcacca ggagtagac aaaatacca 
        ccagatgca gttaaaata gtagcaaca tgtgcccca 
        aactggctg ttgtcgata atgaatggc ctacttcaa 
        gttcaaaac tttcctgat gaatggaaa atagctaaa 
        attgtatta atcttaaaa cctggcaaa ccaacagaa 
        atagcagca agttcatat agaccactc tgtatgcta 
        aactctcta agcaagctg ctggaaact ctcattaaa 
        aacagacta caaagtgaa ttagttaat agaggtgga 
        ttacatcca cgacagtac ggttttcaa aaaggaaaa 
        tccacaatt caagcagta gaaaaggca ctagcgatt 
        gcaagagat tttaacaga aaatattgc acacttata 
        acaattgat gtgaagaac gcatttaac tctgctagt 
        cataaaatt attattgaa aaactgcag gaaaaaaga 
        ataccgaaa tacttgata aatttcatc tcaagctac 
        ctaaaaaat agaaagatt atgattaaa ccgggaagt 
        atgataaaa gtgacggct ggagttcca cagggtttg 
        gtccttgga ccaacactg tggaatatt ctatatgat 
        gaaatctta agcattgaa cttactagt gatgctaaa 
        actataggg tttgctgac gaccttaca ctaattgtt 
        ggagcatcc gaagataag acttttgta aacaataca 
        aatgagtgt ctagcaaga attaacaag tggatgaag 
        tctcacaag ttgcagtta gctcctaat aaaaccgag 
        gcagtttta cttagaggt atgcaaaag agggaacgc 
        attatgttt acgattgaa ggagtcaac attacaccg 
        agcaaaacg ttaaaatac cttggaatt ataattgat 
        gagagactt acctttgga agtcatgtt gagacagct 
        attttaaaa gcggaaaaa aacatatct actctaact 
        agaatctta ccaaacata ggaggtcca agagatagc 
        aaaagaaaa gtattgtgc agtgccgtc caaaacatt 
        gtattgtat ggtgcgcca gtttgggag aaagcacta 
        gaaaccaaa aagcaatac acagccata cacaagcta 
        caaagaaag atgctattg cgagttgcc tcagccttc 
        aaaaccgta tctgggctg gcgctacaa actgtcacg 
        ggcattctc cctatagat attatggta caagaacgg 
        tcatatatg aatcaacat aggctagag gataaaaaa 
        caaactgag atctccgct agagaaaga tctctaaag 
        caatgggaa aataggtgg atgagagaa acgaataag 
        acacaatgg acaaagagg ttaattcca aatttgagg 
        atctggctt aattgcaaa catcggcag acggactac 
        tatcttacg gaagttctt acagaacat ggtgtattg 
        agaagctac gcaaaaaga tttgcgaaa gacaccgtg 
        gaagaatgc attttctgc agtagaaac gacactgtt 
        gaacatact atttttgaa tgtgaaaaa tggaataat 
        attagagag agcacttac aaccaactg ggatgcaca 
        ctagaacct ggaaatctt gtgcagaag atgattgac 
        aacaaaaca aattgggat acaatttac aacatgatc 
        aaacatata atgaaacga aaggaaaag gctgaaatt 
        aacagacag gacgaaatg aaaagtaga tag
      mRNA sequence
      • >TC005297 RA
        aaagaacaa gctaaagca acaaatgaa aatagacga 
        aggcatgca agtgctgca tgcccgacg cgttaaata 
        ccctaaatg actctaaaa atactacaa gccaacgtt 
        ggacgagca tatgcagtt caagatcta gtctgtgcc 
        acagcaaac cagctaaaa gctgatttg ctagttatc 
        agcgaacca aataaaaaa agaatagaa aaatcaaac 
        tggattaaa gacaaaatg agagacgtg gcaatgtta 
        gtcctaaca aagaatgtt ggtattgta agtcatact 
        atttttgac ggttactta atgatccaa acaaaaaaa 
        cttaatata attggatgc tatatatca cccaatata 
        ggaatacaa gattaccaa aaaaaggta gatagcata 
        atgaaggaa gttaaatat ggagaaacc atagtattg 
        ggagagttt aacggcaaa tctccgcta tggggatca 
        cctaaaaaa gatgaaaag ggcgactac tgggtgcag 
        tggatctcg gcgcgcgac atggttgtt ctgaatact 
        ggactaaga cctaccttt gtacgggga gaaagcgaa 
        tcatatatc gacgtaaca atggccact aataaaatc 
        gcaggagat gtaactaat tgggagatt ctggacatc 
        gagacactg acagagcat aaatacatc tactttgaa 
        attaggaca aattctgga aaaataata agggatgtg 
        ggcaacaaa cgaatagta gcaaaatat gcaaatacc 
        ttaagacca tacgaatta gatattgaa aagaaaaag 
        caggtagta acagacctt ttcccaaca gaaaaggaa 
        gagtgggcg atctttcaa atagatgac aaaccaaca 
        caattcaca ctagaggag ctgagaact gcagcggtt 
        ggcataaaa tgcgggaag gcaccagga gtagacaaa 
        ataccacca gatgcagtt aaaatagta gcaacatgt 
        gccccaaac tggctgttg tcgataatg aatggccta 
        cttcaagtt caaaacttt cctgatgaa tggaaaata 
        gctaaaatt gtattaatc ttaaaacct ggcaaacca 
        acagaaata gcagcaagt tcatataga ccactctgt 
        atgctaaac tctctaagc aagctgctg gaaactctc 
        attaaaaac agactacaa agtgaatta gttaataga 
        ggtggatta catccacga cagtacggt tttcaaaaa 
        ggaaaatcc acaattcaa gcagtagaa aaggcacta 
        gcgattgca agagatttt aacagaaaa tattgcaca 
        cttataaca attgatgtg aagaacgca tttaactct 
        gctagtcat aaaattatt attgaaaaa ctgcaggaa 
        aaaagaata ccgaaatac ttgataaat ttcatctca 
        agctaccta aaaaataga aagattatg attaaaccg 
        ggaagtatg ataaaagtg acggctgga gttccacag 
        ggtttggtc cttggacca acactgtgg aatattcta 
        tatgatgaa atcttaagc attgaactt actagtgat 
        gctaaaact atagggttt gctgacgac cttacacta 
        attgttgga gcatccgaa gataagact tttgtaaac 
        aatacaaat gagtgtcta gcaagaatt aacaagtgg 
        atgaagtct cacaagttg cagttagct cctaataaa 
        accgaggca gttttactt agaggtatg caaaagagg 
        gaacgcatt atgtttacg attgaagga gtcaacatt 
        acaccgagc aaaacgtta aaatacctt ggaattata 
        attgatgag agacttacc tttggaagt catgttgag 
        acagctatt ttaaaagcg gaaaaaaac atatctact 
        ctaactaga atcttacca aacatagga ggtccaaga 
        gatagcaaa agaaaagta ttgtgcagt gccgtccaa 
        aacattgta ttgtatggt gcgccagtt tgggagaaa 
        gcactagaa accaaaaag caatacaca gccatacac 
        aagctacaa agaaagatg ctattgcga gttgcctca 
        gccttcaaa accgtatct gggctggcg ctacaaact 
        gtcacgggc attctccct atagatatt atggtacaa 
        gaacggtca tatatgaat caacatagg ctagaggat 
        aaaaaacaa actgagatc tccgctaga gaaagatct 
        ctaaagcaa tgggaaaat aggtggatg agagaaacg 
        aataagaca caatggaca aagaggtta attccaaat 
        ttgaggatc tggcttaat tgcaaacat cggcagacg 
        gactactat cttacggaa gttcttaca gaacatggt 
        gtattgaga agctacgca aaaagattt gcgaaagac 
        accgtggaa gaatgcatt ttctgcagt agaaacgac 
        actgttgaa catactatt tttgaatgt gaaaaatgg 
        aataatatt agagagagc acttacaac caactggga 
        tgcacacta gaacctgga aatcttgtg cagaagatg 
        attgacaac aaaacaaat tgggataca atttacaac 
        atgatcaaa catataatg aaacgaaag gaaaaggct 
        gaaattaac agacaggac gaaatgaaa agtagatag 
        aagaaaaca gaagaacaa aa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY