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Gene information
General information
TC000682 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7621756..7624880
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000682
    iBeetle screen iB_10543

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000682 RA
      MRILGFNFD PWDTPWRKR LHFLAAGAW VFVFLFGGF 
      VVLLLCIYL IFTPLRWLV LFYFCWIYY DRNTKDRGG 
      RPIEWIKNF KWYKYTAEH FLITVKLAP GFQLDSNKN 
      YLFACFPHG VIPMGAYHA IACNHSRFR QLYKNFNFK 
      VAILLIQLL FPLTRELNL AQGGISASS ESLNYALSR 
      PQGGHIVLL FPGGALEAT YTKPGFYKF VVKKRKGFV 
      RVALQNGVP LVPVITFGE NDLYNIIGD NYYWRMFQN 
      ITRKLTRDF QLNSNQNYL FACFPHGVV PLGLYSTIV 
      NSHSKFRNL YPNFRVKLA ITPFLFVFP FTRELAMAL 
      DFISCSFKS LMRVLSKPE GGEIVVLFP GGALECSYN 
      QYQPQFYKC VLNRRKGFV RVALKSGAA LVPVLTFGE 
      NDLLTVENS FWQKFRFIT ERYQTFACG FVHGRGVFQ 
      STFGMVPRR KPTMTVVGT PIATAKTEN PSDAQVDAL 
      HKKFQEELE KLFEKYKHQ FFDNPQDKC LEFV
    CDS sequence
    • >TC000682 RA
      atgagaatt ttaggcttc aattttgat ccctgggac 
      accccttgg cggaaaagg ctccacttt ctagcagcc 
      ggtgcttgg gtgtttgtg ttcttgttt ggcggattt 
      gtcgtgcta cttttatgc atctactta atatttact 
      cctttacgg tggttagtt ttgttctac ttctgttgg 
      atttattac gacaggaat actaaagat aggggaggt 
      agacctatc gaatggata aaaaacttc aagtggtac 
      aaatacact gctgagcat ttcctcata accgtaaaa 
      ctcgcacct ggcttccaa cttgactca aataaaaat 
      tacttattt gcatgtttt ccgcatgga gttatacct 
      atgggggcc tatcatgca atcgcctgt aatcattca 
      cgatttcgg cagttgtat aaaaatttt aattttaaa 
      gtagcgatc cttttgatt cagctttta ttcccttta 
      acacgagaa ttaaacctg gcacaagga ggcatctct 
      gcatcgtct gaatcgctc aattacgct ttaagtaga 
      ccgcaaggt ggacacatt gtgcttctt tttcctgga 
      ggtgcttta gaagctact tacacaaaa ccaggattt 
      tacaaattc gttgtcaag aaaaggaaa ggttttgtc 
      agagtcgcg ctacaaaat ggggttcct ttggtaccg 
      gttataact ttcggagaa aatgatttg tataatata 
      attggagat aattactat tggcgaatg tttcaaaat 
      attaccagg aagctcact cgtgatttt caactaaac 
      tcaaaccag aattatctc ttcgcctgc tttcctcac 
      ggagttgtc ccgttgggg ctttattca actatcgtc 
      aactcgcat tcgaaattt cgaaattta tacccaaat 
      tttcgagtg aaactcgct ataactcca tttcttttt 
      gtcttcccc tttactcga gaacttgca atggcttta 
      gacttcatc tcatgttcg ttcaaatct cttatgagg 
      gtgttaagc aaaccagaa ggtggagaa attgtagtg 
      ttatttccc ggaggtgca ttggaatgt tcttataat 
      cagtaccag cctcaattt tataagtgc gtcctaaac 
      cgcagaaaa ggcttcgtg agagtggct ttaaaaagc 
      ggagctgcg ttggtcccg gttttaact ttcggcgag 
      aacgatcta ttgactgtt gagaacagt ttttggcaa 
      aaatttcgg ttcattaca gaaaggtat cagactttt 
      gcttgtggt tttgttcat gggagagga gttttccaa 
      agcactttt gggatggtt ccgagacga aaaccgaca 
      atgactgtt gttggtaca ccaattgca actgcgaaa 
      acagaaaat ccaagtgat gcacaagtc gacgccctc 
      cacaagaaa tttcaagaa gagttagag aaactgttt 
      gaaaaatac aaacaccaa ttttttgac aatcctcaa 
      gataaatgt ttagaattt gtgtaa
    mRNA sequence
    • >TC000682 RA
      atgagaatt ttaggcttc aattttgat ccctgggac 
      accccttgg cggaaaagg ctccacttt ctagcagcc 
      ggtgcttgg gtgtttgtg ttcttgttt ggcggattt 
      gtcgtgcta cttttatgc atctactta atatttact 
      cctttacgg tggttagtt ttgttctac ttctgttgg 
      atttattac gacaggaat actaaagat aggggaggt 
      agacctatc gaatggata aaaaacttc aagtggtac 
      aaatacact gctgagcat ttcctcata accgtaaaa 
      ctcgcacct ggcttccaa cttgactca aataaaaat 
      tacttattt gcatgtttt ccgcatgga gttatacct 
      atgggggcc tatcatgca atcgcctgt aatcattca 
      cgatttcgg cagttgtat aaaaatttt aattttaaa 
      gtagcgatc cttttgatt cagctttta ttcccttta 
      acacgagaa ttaaacctg gcacaagga ggcatctct 
      gcatcgtct gaatcgctc aattacgct ttaagtaga 
      ccgcaaggt ggacacatt gtgcttctt tttcctgga 
      ggtgcttta gaagctact tacacaaaa ccaggattt 
      tacaaattc gttgtcaag aaaaggaaa ggttttgtc 
      agagtcgcg ctacaaaat ggggttcct ttggtaccg 
      gttataact ttcggagaa aatgatttg tataatata 
      attggagat aattactat tggcgaatg tttcaaaat 
      attaccagg aagctcact cgtgatttt caactaaac 
      tcaaaccag aattatctc ttcgcctgc tttcctcac 
      ggagttgtc ccgttgggg ctttattca actatcgtc 
      aactcgcat tcgaaattt cgaaattta tacccaaat 
      tttcgagtg aaactcgct ataactcca tttcttttt 
      gtcttcccc tttactcga gaacttgca atggcttta 
      gacttcatc tcatgttcg ttcaaatct cttatgagg 
      gtgttaagc aaaccagaa ggtggagaa attgtagtg 
      ttatttccc ggaggtgca ttggaatgt tcttataat 
      cagtaccag cctcaattt tataagtgc gtcctaaac 
      cgcagaaaa ggcttcgtg agagtggct ttaaaaagc 
      ggagctgcg ttggtcccg gttttaact ttcggcgag 
      aacgatcta ttgactgtt gagaacagt ttttggcaa 
      aaatttcgg ttcattaca gaaaggtat cagactttt 
      gcttgtggt tttgttcat gggagagga gttttccaa 
      agcactttt gggatggtt ccgagacga aaaccgaca 
      atgactgtt gttggtaca ccaattgca actgcgaaa 
      acagaaaat ccaagtgat gcacaagtc gacgccctc 
      cacaagaaa tttcaagaa gagttagag aaactgttt 
      gaaaaatac aaacaccaa ttttttgac aatcctcaa 
      gataaatgt ttagaattt gtgtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY