Status
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Gene information
General information
TC014437 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10420497..10422619
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014437
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10538

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014437 RA
      MPPKVTTNL LKQLRGLMQ NPQYVSETI NAYIVPSND 
      AHNSEYLAD CDMFRGFIT GFTGSAGTA IITEKEALL 
      WTDGRYFLQ ASQQLDSNW TLMKEGIPS TPTQGDWLC 
      KNLPSGSRV GVDPNLYTH HIWMPLQSR LAQAGHKLV 
      PVNKNLVEV LWTDRPARP TNPVRPLGL EFAGKSVGD 
      KLSKVRADM EREKVDFLV LTALDEIAW LLNLRGSDI 
      EYNPVFFSY VVVHKDKFT VFLDPKQTT DEVKQHLTK 
      EAGNNYEIK PYTEIVNYL KQNCSKIDG FAWFSEDAS 
      YALTSLIPS KSLLTEVTP VPLMKAVKN PTEIKGMRN 
      AHLKDGAAL CCYFSWLEK NVANGGITE VSGAKKLDE 
      FRALQADFV GPSFATISS VGPHGAIIH YHPEPETDV 
      PITTDTLYL CDSGGQYKD GTTDVTRTF HFGTPTEYE 
      KECFTRVLK GQIKLATSI FPSKIKGNY LDSFAREFL 
      WEVGLDYAH GTGHGIGSY LNVHEGPMG ISWRLIADD 
      PGLESGMFL SNEPGYYED GKFGIRLED IVQIVPANP 
      PHNFNDRGF LTFETITFC PKQTKLILV DLLTDKELA 
      YLNAYHKQC RDLLGPILE KQGQVEAKE WLWRETEPL 
      KRN
    CDS sequence
    • >TC014437 RA
      atgcctcca aaagtgacc accaatctg ctgaagcaa 
      ctgcgaggg ttgatgcaa aacccccag tatgtcagt 
      gagacaatc aacgcgtat atcgtcccc tcaaacgac 
      gctcacaac agcgaatac ttggccgat tgtgacatg 
      tttaggggc ttcattacc ggcttcacc ggttcagct 
      ggaactgcc ataataaca gaaaaagag gcccttttg 
      tggaccgac ggccgctat ttcctccaa gcgtcgcaa 
      caattggat tccaattgg actctaatg aaagagggg 
      attccgtca accccgact caaggtgac tggttgtgc 
      aagaattta ccctcagga tcacgtgta ggggtggac 
      cccaacctc tacacacac cacatatgg atgcctctg 
      cagtcccga ttagcccaa gccgggcat aagcttgtc 
      ccagttaac aaaaaccta gtcgaggtt ttatggact 
      gacaggccc gcaagaccc acaaatccg gtccgtcct 
      ttaggccta gagttcgcc gggaaaagt gttggggac 
      aaattgtcc aaagtcagg gccgatatg gagcgagaa 
      aaggtcgat tttttagtt ctgacggcg cttgacgaa 
      atcgcgtgg ttgctcaat ttgcggggc tcagatatc 
      gaatacaac ccagttttt ttctcttat gtggtcgtc 
      cacaaagac aagtttacg gtattttta gaccccaag 
      caaacaaca gatgaagtt aagcaacat ttgacgaaa 
      gaagcggga aataattac gaaatcaag ccgtacacc 
      gaaatcgtg aattatttg aaacaaaac tgttcgaaa 
      atcgacggc tttgcgtgg ttttccgag gacgcgagc 
      tacgccctt acgagtctt atcccctct aagtcgctc 
      ctgactgaa gtaaccccg gtcccgtta atgaaagcc 
      gtgaaaaac cccaccgaa atcaagggt atgcgtaac 
      gcacatttg aaagacggg gcagccctt tgttgctat 
      ttctcctgg ttggagaaa aacgtggca aatgggggg 
      atcaccgaa gtgtcgggg gcgaaaaag ttggacgaa 
      ttccgggcg ctacaggcg gattttgtc ggcccgagc 
      ttcgcgact ataagttcg gtgggtccc catggggct 
      atcattcac taccacccg gagcccgaa actgacgtt 
      cccataacc accgatacg ttgtatttg tgcgactct 
      gggggccag tataaggat ggcaccact gatgtgact 
      aggactttt cactttggg actcccacc gagtacgag 
      aaggagtgt tttaccagg gttctgaag gggcaaatt 
      aagttggcg acttcgatt ttcccgtcg aagatcaag 
      gggaattac ctggattcg ttcgcgagg gagtttctg 
      tgggaggtg ggactggat tatgcccac gggacggga 
      catgggatc ggttcgtat ctcaacgtc catgaagga 
      ccaatgggg atttcctgg cgattgatc gccgatgat 
      cccggattg gagtcgggg atgttcttg tcaaacgaa 
      ccggggtac tacgaggat gggaaattc gggattagg 
      ttggaggat atcgtccag atcgttcct gcaaacccc 
      ccacataat ttcaatgat cgggggttc ttgaccttt 
      gagaccatt acgttctgt ccgaaacaa acaaaattg 
      attttggtt gatttgttg actgataag gagttggcg 
      tatttgaat gcgtatcac aagcagtgt agggacttg 
      ttggggccc atcttggag aagcaggga caggtggag 
      gcgaaggag tggctgtgg agggagacg gagccgttg 
      aagaggaac taa
    mRNA sequence
    • >TC014437 RA
      ttgttaaaa accgacctg caccttgca ctagtgtca 
      ctagtttga cagttgtcc caattaaca cgtcagata 
      attcggaca ataaagacc ggcacttat cttatcaat 
      tgctcctaa tgtaaaaca cagagcccc agaatgcct 
      ccaaaagtg accaccaat ctgctgaag caactgcga 
      gggttgatg caaaacccc cagtatgtc agtgagaca 
      atcaacgcg tatatcgtc ccctcaaac gacgctcac 
      aacagcgaa tacttggcc gattgtgac atgtttagg 
      ggcttcatt accggcttc accggttca gctggaact 
      gccataata acagaaaaa gaggccctt ttgtggacc 
      gacggccgc tatttcctc caagcgtcg caacaattg 
      gattccaat tggactcta atgaaagag gggattccg 
      tcaaccccg actcaaggt gactggttg tgcaagaat 
      ttaccctca ggatcacgt gtaggggtg gaccccaac 
      ctctacaca caccacata tggatgcct ctgcagtcc 
      cgattagcc caagccggg cataagctt gtcccagtt 
      aacaaaaac ctagtcgag gttttatgg actgacagg 
      cccgcaaga cccacaaat ccggtccgt cctttaggc 
      ctagagttc gccgggaaa agtgttggg gacaaattg 
      tccaaagtc agggccgat atggagcga gaaaaggtc 
      gatttttta gttctgacg gcgcttgac gaaatcgcg 
      tggttgctc aatttgcgg ggctcagat atcgaatac 
      aacccagtt tttttctct tatgtggtc gtccacaaa 
      gacaagttt acggtattt ttagacccc aagcaaaca 
      acagatgaa gttaagcaa catttgacg aaagaagcg 
      ggaaataat tacgaaatc aagccgtac accgaaatc 
      gtgaattat ttgaaacaa aactgttcg aaaatcgac 
      ggctttgcg tggttttcc gaggacgcg agctacgcc 
      cttacgagt cttatcccc tctaagtcg ctcctgact 
      gaagtaacc ccggtcccg ttaatgaaa gccgtgaaa 
      aaccccacc gaaatcaag ggtatgcgt aacgcacat 
      ttgaaagac ggggcagcc ctttgttgc tatttctcc 
      tggttggag aaaaacgtg gcaaatggg gggatcacc 
      gaagtgtcg ggggcgaaa aagttggac gaattccgg 
      gcgctacag gcggatttt gtcggcccg agcttcgcg 
      actataagt tcggtgggt ccccatggg gctatcatt 
      cactaccac ccggagccc gaaactgac gttcccata 
      accaccgat acgttgtat ttgtgcgac tctgggggc 
      cagtataag gatggcacc actgatgtg actaggact 
      tttcacttt gggactccc accgagtac gagaaggag 
      tgttttacc agggttctg aaggggcaa attaagttg 
      gcgacttcg attttcccg tcgaagatc aaggggaat 
      tacctggat tcgttcgcg agggagttt ctgtgggag 
      gtgggactg gattatgcc cacgggacg ggacatggg 
      atcggttcg tatctcaac gtccatgaa ggaccaatg 
      gggatttcc tggcgattg atcgccgat gatcccgga 
      ttggagtcg gggatgttc ttgtcaaac gaaccgggg 
      tactacgag gatgggaaa ttcgggatt aggttggag 
      gatatcgtc cagatcgtt cctgcaaac cccccacat 
      aatttcaat gatcggggg ttcttgacc tttgagacc 
      attacgttc tgtccgaaa caaacaaaa ttgattttg 
      gttgatttg ttgactgat aaggagttg gcgtatttg 
      aatgcgtat cacaagcag tgtagggac ttgttgggg 
      cccatcttg gagaagcag ggacaggtg gaggcgaag 
      gagtggctg tggagggag acggagccg ttgaagagg 
      aactaagac caaacagta tcgtgcttg ccatacttt 
      atgatttat tattggaaa atgtaacaa tgttcacaa 
      taaaatgtt tttaaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY