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Gene information
General information
TC003288 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 798353..802724
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003288
    iBeetle screen iB_10533

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003288 RA
      MKIATSFNS KVPEGKPLC QYLAAFTGT LTIVTSGMH 
      YGWPSPSLP ILERLENST LTMNHSEGS WMAVMPLLG 
      ALIGSLLAA TVVDILGRK RAILLTCFP FFAAWIMIA 
      FSQSLTVLY IARFIAGIA DGWAFTAVP MYIGEIADP 
      KIRGLLGSG VSSSWIFGI LLINAIGSY LSITITALV 
      SSIVPVLTL LTFVWMPES PYYLVMRGH KEEAKCNLQ 
      RLRGLEDVD SELTRVSLA VKAQTQNSG KFLDLFVTK 
      SNRKAVYII MALRGAQQL SGTTAITFY TQLIFEEAG 
      DDISSELAT VIYFSVQFL LTILCSSIV DKAGRRPLL 
      VLSLTGSAC ALFLEGTYF FIKTQTAID VSSFTCIPV 
      ISLIGFVIF FSSGMQSIP ILMLGELFP ANVKAFALC 
      LADIYFCLM ATVVSKFFQ IVKDSFGIY VPFYVFTGS 
      CLLGLVFIV LFVPETKGK SLEEIQQYL GGKTGKEGE 
      EKSESEVV
    CDS sequence
    • >TC003288 RA
      atgaagatt gccactagt ttcaactcc aaagtgccc 
      gagggcaag cccttgtgc cagtatttg gccgccttc 
      accggaacc ttgaccatc gtcaccagc ggcatgcac 
      tacggctgg ccctccccc tcgctccca atcctggaa 
      cgcctggag aactccacc ttgaccatg aaccactct 
      gaaggctcc tggatggcc gtcatgccc ctcctcggc 
      gccctcatc ggctcgctc ctcgccgcc accgtcgtc 
      gacatcctc ggccgcaaa cgcgccatc ctcctcacc 
      tgcttcccc ttcttcgcc gcctggata atgatcgcc 
      ttctcccaa tccctcact gtcttatac atcgcacgt 
      ttcatcgcc ggcatcgcc gacggctgg gccttcact 
      gccgtcccc atgtacatc ggcgagatc gccgacccc 
      aaaatccgc ggcctacta ggatcaggc gtctccagc 
      tcctggatc ttcggaatt ctcctaatc aacgcaatc 
      ggttcatac ttgagcatc accataacg gcacttgtc 
      tcgtcaatt gtgccagtg ctgacgctc ttaacgttt 
      gtctggatg ccggaaagt ccgtactat ttggtgatg 
      cggggccac aaggaggag gccaagtgt aacttgcag 
      cgtcttcga ggcttggag gacgtggac agtgagttg 
      acgcgggtt agtttggct gtgaaggca caaacgcaa 
      aacagcggg aaattcctc gatttattc gtgacgaaa 
      agtaacagg aaagctgtt tacattata atggcacta 
      cgtggggca caacaactg agcggcacc acggccatc 
      accttctac acccaactc atcttcgaa gaagctgga 
      gatgacatt tcctccgaa ctcgccaca gttatctat 
      ttctccgtc cagttcctt ctcactatt ctgtgttca 
      agtattgtg gacaaggcc ggccgaagg ccgctcctg 
      gtcctttcg ttaaccggc tcggcttgt gccttgttc 
      ctcgaaggc acttatttc ttcattaaa acacaaact 
      gccatagat gtgtcgtcc ttcacctgt attcccgtc 
      atttccctg atcgggttt gtgattttc ttcagttct 
      ggaatgcag tccatcccg attttgatg ctaggggag 
      ttgttccca gccaatgtc aaagcgttc gccctgtgt 
      ttggccgat atttatttt tgtttgatg gccacggtc 
      gtgtcaaag ttcttccag atagtgaaa gattccttc 
      gggatctat gtcccgttt tacgttttc acgggcagt 
      tgtttgctg gggttggtt tttatcgtg ttgtttgtg 
      ccggagact aagggcaaa agtctggag gagatacag 
      caatatttg ggggggaaa acggggaag gagggcgag 
      gagaaaagc gaaagtgaa gtcgtgtga
    mRNA sequence
    • >TC003288 RA
      ccggtgttt cactcccat tcattcgta cagtaaacc 
      ctcactcgc gactatcat gttgaaaaa aaactcaaa 
      agggaccaa atcttaaca caatgaaga ttgccacta 
      gtttcaact ccaaagtgc ccgagggca agcccttgt 
      gccagtatt tggccgcct tcaccggaa ccttgacca 
      tcgtcacca gcggcatgc actacggct ggccctccc 
      cctcgctcc caatcctgg aacgcctgg agaactcca 
      ccttgacca tgaaccact ctgaaggct cctggatgg 
      ccgtcatgc ccctcctcg gcgccctca tcggctcgc 
      tcctcgccg ccaccgtcg tcgacatcc tcggccgca 
      aacgcgcca tcctcctca cctgcttcc ccttcttcg 
      ccgcctgga taatgatcg ccttctccc aatccctca 
      ctgtcttat acatcgcac gtttcatcg ccggcatcg 
      ccgacggct gggccttca ctgccgtcc ccatgtaca 
      tcggcgaga tcgccgacc ccaaaatcc gcggcctac 
      taggatcag gcgtctcca gctcctgga tcttcggaa 
      ttctcctaa tcaacgcaa tcggttcat acttgagca 
      tcaccataa cggcacttg tctcgtcaa ttgtgccag 
      tgctgacgc tcttaacgt ttgtctgga tgccggaaa 
      gtccgtact atttggtga tgcggggcc acaaggagg 
      aggccaagt gtaacttgc agcgtcttc gaggcttgg 
      aggacgtgg acagtgagt tgacgcggg ttagtttgg 
      ctgtgaagg cacaaacgc aaaacagcg ggaaattcc 
      tcgatttat tcgtgacga aaagtaaca ggaaagctg 
      tttacatta taatggcac tacgtgggg cacaacaac 
      tgagcggca ccacggcca tcaccttct acacccaac 
      tcatcttcg aagaagctg gagatgaca tttcctccg 
      aactcgcca cagttatct atttctccg tccagttcc 
      ttctcacta ttctgtgtt caagtattg tggacaagg 
      ccggccgaa ggccgctcc tggtccttt cgttaaccg 
      gctcggctt gtgccttgt tcctcgaag gcacttatt 
      tcttcatta aaacacaaa ctgccatag atgtgtcgt 
      ccttcacct gtattcccg tcatttccc tgatcgggt 
      ttgtgattt tcttcagtt ctggaatgc agtccatcc 
      cgattttga tgctagggg agttgttcc cagccaatg 
      tcaaagcgt tcgccctgt gtttggccg atatttatt 
      tttgtttga tggccacgg tcgtgtcaa agttcttcc 
      agatagtga aagattcct tcgggatct atgtcccgt 
      tttacgttt tcacgggca gttgtttgc tggggttgg 
      tttttatcg tgttgtttg tgccggaga ctaagggca 
      aaagtctgg aggagatac agcaatatt tggggggga 
      aaacgggga aggagggcg aggagaaaa gcgaaagtg 
      aagtcgtgt gataagact gattgttgc gttcgttat 
      tgttttata ttttgtact gtgagctag gctaagtga 
      tattttttc tatgaaaaa atattttat taaattaag 
      tttgatata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY