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Gene information
General information
TC001022 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9917390..9919474
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001022
    iBeetle screen iB_10527

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001022 RA
      MHIIYLFVG LYTVIGAPV EKSMYDAIQ EKCPSVSSI 
      PTFKSQPLF RICKDWKNI SEISQLTET NAAAIRCLL 
      YYQTFTEYC TQTQTINTS VSLKLEYDL DTLCDFVKS 
      MPHKNLSSI FATRDNCEK ICTDELELP HSVCKMSYY 
      FAHLDVTPA EKLEKPVPL KPISTDKNT DQSPKTEIK 
      PHPDILQPN PAPPTSINA APSANKVVQ ETTSQKQKA 
      KAPTAVQPP HIENLPNSE IKPDNTNPV VVPEKNAPQ 
      TDQNRQKAQ PEQTSVENQ NPAPQETKK VDPIEPKPP 
      QDDKKVELN PGKSDNQQD LPQEEKILD DEDDEEQPP 
      DTLENDLAE SSLNAGPGS KPSKLDESE PVNYSTEME 
      NDSYFFTYF MVLCVIFIA GYVCYHNKQ KILALILEG 
      RRGKRQYRG RRPNSANYH KLDSNLEEA ISSSCKKNT 
      SNVIY
    • >TC001022 RB
      MHIIYLFVG LYTVIGAPV EKSMYDAIQ EKCPSVSSI 
      PTFKSQPLF RICKDWKNI SEISQLTET NAAAIRCLL 
      YYQTFTEYC TQTQTINTS VSLKLEYDL DTLCDFVKS 
      MPHKNLSSI FATRDNCEK ICTDELELP HSVCKMSYY 
      FAHLDVTPA EKLEKPVPL KPISTDKNT DQSPKTEIK 
      PHPDILQPN PAPPTSINA APSANKVVQ ETTSQKQKA 
      KAPTAVQPP HIENLPNSE IKPDNTNPV VVPEKNAPQ 
      TDQNRQKAQ PEQTSVENQ NPAPQETKK VDPIEPKPP 
      QDDKKVELN PGKSDNQQD LPQEEKILD DEDDEEQPP 
      DTQSSLNAG PGSKPSKLD ESEPVNYST EMENDSYFF 
      TYFMVLCVI FIAGYVCYH NKQKILALI LEGRRGKRQ 
      YRGRRPNSA NYHKLDSNL EEAISSSCK KNTSNVIY
    CDS sequence
    • >TC001022 RA
      atgcacatt atttattta ttcgtgggg ctttacacc 
      gtcattggt gcacctgtg gagaagtca atgtatgac 
      gctattcaa gaaaagtgt ccgagtgtt tcctcaatt 
      cctacattt aagtcccag ccattattt agaatttgc 
      aaagactgg aagaatatt agcgaaata agtcaactg 
      acagaaacc aatgcagca gcaatccgt tgcttgctt 
      tattatcaa acatttacc gaatattgt actcagact 
      cagactatt aacacgagt gtgtctctc aaactggaa 
      tacgatctt gatactttg tgtgatttt gtaaaaagt 
      atgccacat aagaattta agctcgatt tttgcaact 
      cgggacaat tgtgaaaaa atctgcact gatgaactt 
      gagttgcct cactcagta tgtaagatg tcttattat 
      tttgcacat ctggacgtg acgcctgca gaaaaactc 
      gaaaaacct gtcccttta aaacccatt agcactgat 
      aaaaatact gatcaatct ccaaaaact gaaattaaa 
      cctcatcct gatatttta caacccaat cccgcacct 
      ccaacttct ataaatgct gcgccaagt gcaaataaa 
      gtagtgcaa gaaacaacg tcacaaaaa cagaaagcc 
      aaagctcca actgctgta caaccgcct catattgaa 
      aacctacca aacagtgag attaaaccc gataatacg 
      aatcctgta gtggtgcca gaaaaaaat gcacctcaa 
      acagatcag aaccggcaa aaagcccaa ccggagcaa 
      acttctgta gaaaatcaa aatcctgct cctcaggaa 
      actaaaaaa gttgaccca attgagcca aaaccgcct 
      caagatgat aaaaaagtt gagctaaat cctgggaaa 
      agtgacaat cagcaggat ttgccgcag gaggagaaa 
      attcttgat gatgaagac gacgaggag cagccacca 
      gatacacta gagaatgat cttgcagag tcgtccctg 
      aacgctggt cccggctcg aaaccctca aaactcgac 
      gagtccgaa cctgtgaat tactccacg gaaatggaa 
      aacgattct tatttcttt acgtatttt atggtctta 
      tgtgtcatt ttcatcgcc ggttatgtg tgttatcac 
      aataaacaa aagattctc gcattgatt ttagaagga 
      cgtaggggt aaaagacag tatagggga agacgaccg 
      aattctgcc aattatcac aaattggac agtaatttg 
      gaagaggcg atcagctcg tcgtgtaag aaaaacacg 
      agtaatgtg atttactga
    • >TC001022 RB
      atgcacatt atttattta ttcgtgggg ctttacacc 
      gtcattggt gcacctgtg gagaagtca atgtatgac 
      gctattcaa gaaaagtgt ccgagtgtt tcctcaatt 
      cctacattt aagtcccag ccattattt agaatttgc 
      aaagactgg aagaatatt agcgaaata agtcaactg 
      acagaaacc aatgcagca gcaatccgt tgcttgctt 
      tattatcaa acatttacc gaatattgt actcagact 
      cagactatt aacacgagt gtgtctctc aaactggaa 
      tacgatctt gatactttg tgtgatttt gtaaaaagt 
      atgccacat aagaattta agctcgatt tttgcaact 
      cgggacaat tgtgaaaaa atctgcact gatgaactt 
      gagttgcct cactcagta tgtaagatg tcttattat 
      tttgcacat ctggacgtg acgcctgca gaaaaactc 
      gaaaaacct gtcccttta aaacccatt agcactgat 
      aaaaatact gatcaatct ccaaaaact gaaattaaa 
      cctcatcct gatatttta caacccaat cccgcacct 
      ccaacttct ataaatgct gcgccaagt gcaaataaa 
      gtagtgcaa gaaacaacg tcacaaaaa cagaaagcc 
      aaagctcca actgctgta caaccgcct catattgaa 
      aacctacca aacagtgag attaaaccc gataatacg 
      aatcctgta gtggtgcca gaaaaaaat gcacctcaa 
      acagatcag aaccggcaa aaagcccaa ccggagcaa 
      acttctgta gaaaatcaa aatcctgct cctcaggaa 
      actaaaaaa gttgaccca attgagcca aaaccgcct 
      caagatgat aaaaaagtt gagctaaat cctgggaaa 
      agtgacaat cagcaggat ttgccgcag gaggagaaa 
      attcttgat gatgaagac gacgaggag cagccacca 
      gatacacag tcgtccctg aacgctggt cccggctcg 
      aaaccctca aaactcgac gagtccgaa cctgtgaat 
      tactccacg gaaatggaa aacgattct tatttcttt 
      acgtatttt atggtctta tgtgtcatt ttcatcgcc 
      ggttatgtg tgttatcac aataaacaa aagattctc 
      gcattgatt ttagaagga cgtaggggt aaaagacag 
      tatagggga agacgaccg aattctgcc aattatcac 
      aaattggac agtaatttg gaagaggcg atcagctcg 
      tcgtgtaag aaaaacacg agtaatgtg atttactga
    mRNA sequence
    • >TC001022 RA
      cggttggtg gcacggatc caaaggaac aagataaaa 
      cttggtata attcaagta aattgtgaa accttacat 
      tgatttaat tgtggatta ggcaagttt gtctgaaag 
      acaatttca tagccccgt atttacgca aaaactgca 
      tttcaacac atcgagttg gggtttttg ccgggtttg 
      gttggcatt gaaagtatc aatgtcaaa caaaatagt 
      aaagttgtg agtgttttc gtgattttt cataataat 
      aacggtgtg aatgagcac aaaaacgtg aatttgggc 
      ttattttag tgtttgtag ttgtgggtt agttaatct 
      ataaaaatg aacaagatg cacattatt tatttattc 
      gtggggctt tacaccgtc attggtgca cctgtggag 
      aagtcaatg tatgacgct attcaagaa aagtgtccg 
      agtgtttcc tcaattcct acatttaag tcccagcca 
      ttatttaga atttgcaaa gactggaag aatattagc 
      gaaataagt caactgaca gaaaccaat gcagcagca 
      atccgttgc ttgctttat tatcaaaca tttaccgaa 
      tattgtact cagactcag actattaac acgagtgtg 
      tctctcaaa ctggaatac gatcttgat actttgtgt 
      gattttgta aaaagtatg ccacataag aatttaagc 
      tcgattttt gcaactcgg gacaattgt gaaaaaatc 
      tgcactgat gaacttgag ttgcctcac tcagtatgt 
      aagatgtct tattatttt gcacatctg gacgtgacg 
      cctgcagaa aaactcgaa aaacctgtc cctttaaaa 
      cccattagc actgataaa aatactgat caatctcca 
      aaaactgaa attaaacct catcctgat attttacaa 
      cccaatccc gcacctcca acttctata aatgctgcg 
      ccaagtgca aataaagta gtgcaagaa acaacgtca 
      caaaaacag aaagccaaa gctccaact gctgtacaa 
      ccgcctcat attgaaaac ctaccaaac agtgagatt 
      aaacccgat aatacgaat cctgtagtg gtgccagaa 
      aaaaatgca cctcaaaca gatcagaac cggcaaaaa 
      gcccaaccg gagcaaact tctgtagaa aatcaaaat 
      cctgctcct caggaaact aaaaaagtt gacccaatt 
      gagccaaaa ccgcctcaa gatgataaa aaagttgag 
      ctaaatcct gggaaaagt gacaatcag caggatttg 
      ccgcaggag gagaaaatt cttgatgat gaagacgac 
      gaggagcag ccaccagat acactagag aatgatctt 
      gcagagtcg tccctgaac gctggtccc ggctcgaaa 
      ccctcaaaa ctcgacgag tccgaacct gtgaattac 
      tccacggaa atggaaaac gattcttat ttctttacg 
      tattttatg gtcttatgt gtcattttc atcgccggt 
      tatgtgtgt tatcacaat aaacaaaag attctcgca 
      ttgatttta gaaggacgt aggggtaaa agacagtat 
      aggggaaga cgaccgaat tctgccaat tatcacaaa 
      ttggacagt aatttggaa gaggcgatc agctcgtcg 
      tgtaagaaa aacacgagt aatgtgatt tactgagtt 
      tgggaattt ttgtaaaat atgtacata aaatagacg 
      catcaaatt ttctaactt tatacggtg tggatagga 
      tttgtgttg agtgttttg tgtttgagg ccatgctag 
      atatgtttc c
    • >TC001022 RB
      cggttggtg gcacggatc caaaggaac aagataaaa 
      cttggtata attcaagta aattgtgaa accttacat 
      tgatttaat tgtggatta ggcaagttt gtctgaaag 
      acaatttca tagccccgt atttacgca aaaactgca 
      tttcaacac atcgagttg gggtttttg ccgggtttg 
      gttggcatt gaaagtatc aatgtcaaa caaaatagt 
      aaagttgtg agtgttttc gtgattttt cataataat 
      aacggtgtg aatgagcac aaaaacgtg aatttgggc 
      ttattttag tgtttgtag ttgtgggtt agttaatct 
      ataaaaatg aacaagatg cacattatt tatttattc 
      gtggggctt tacaccgtc attggtgca cctgtggag 
      aagtcaatg tatgacgct attcaagaa aagtgtccg 
      agtgtttcc tcaattcct acatttaag tcccagcca 
      ttatttaga atttgcaaa gactggaag aatattagc 
      gaaataagt caactgaca gaaaccaat gcagcagca 
      atccgttgc ttgctttat tatcaaaca tttaccgaa 
      tattgtact cagactcag actattaac acgagtgtg 
      tctctcaaa ctggaatac gatcttgat actttgtgt 
      gattttgta aaaagtatg ccacataag aatttaagc 
      tcgattttt gcaactcgg gacaattgt gaaaaaatc 
      tgcactgat gaacttgag ttgcctcac tcagtatgt 
      aagatgtct tattatttt gcacatctg gacgtgacg 
      cctgcagaa aaactcgaa aaacctgtc cctttaaaa 
      cccattagc actgataaa aatactgat caatctcca 
      aaaactgaa attaaacct catcctgat attttacaa 
      cccaatccc gcacctcca acttctata aatgctgcg 
      ccaagtgca aataaagta gtgcaagaa acaacgtca 
      caaaaacag aaagccaaa gctccaact gctgtacaa 
      ccgcctcat attgaaaac ctaccaaac agtgagatt 
      aaacccgat aatacgaat cctgtagtg gtgccagaa 
      aaaaatgca cctcaaaca gatcagaac cggcaaaaa 
      gcccaaccg gagcaaact tctgtagaa aatcaaaat 
      cctgctcct caggaaact aaaaaagtt gacccaatt 
      gagccaaaa ccgcctcaa gatgataaa aaagttgag 
      ctaaatcct gggaaaagt gacaatcag caggatttg 
      ccgcaggag gagaaaatt cttgatgat gaagacgac 
      gaggagcag ccaccagat acacagtcg tccctgaac 
      gctggtccc ggctcgaaa ccctcaaaa ctcgacgag 
      tccgaacct gtgaattac tccacggaa atggaaaac 
      gattcttat ttctttacg tattttatg gtcttatgt 
      gtcattttc atcgccggt tatgtgtgt tatcacaat 
      aaacaaaag attctcgca ttgatttta gaaggacgt 
      aggggtaaa agacagtat aggggaaga cgaccgaat 
      tctgccaat tatcacaaa ttggacagt aatttggaa 
      gaggcgatc agctcgtcg tgtaagaaa aacacgagt 
      aatgtgatt tactgagtt tgggaattt ttgtaaaat 
      atgtacata aaatagacg catcaaatt ttctaactt 
      tatacggtg tggatagga tttgtgttg agtgttttg 
      tgtttgagg ccatgctag atatgtttc c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY