Status
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Gene information
General information
TC034074
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005710,TC005711
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8580230..8583908
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034074
    iBeetle screen iB_10524

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034074 RA
      MVKLKAPPY RVWIAVMVF MSTFTNYML RANMSVSII 
      SMVDKAKKN QTAVCAAPP DNSTKPPPK PKSGSKKFK 
      WSEIDEGWI LAAYFYGYF FTVLPGGII SNYIGPWHT 
      IVWSSLGSA VLTGLTPIC ATSGGVGGV CANRFFIGF 
      LGGPVYPAV NDLIAKWAP PKEKGKFLA AMMGNTLGT 
      VVTWNLVAA ASSALGWEW GFHVLAIFM LVYCAVFGV 
      IVRDRPDIH PWVSEDEKK YIAESQEGR LTTKKGFPP 
      YHKMMCFNF PFWALVIAQ LGNLWGLNL VLTYAPKFM 
      AGTLGFNIK ASAGLAALP YIARLIGSN IFGIIGDYM 
      RKKEIMSVT KIRKFFIIF SHFIPGALL IVMRAFGCI 
      KEGVIAMIV LNQGFNGAC VVSHLVNPQ DLSPNFAGA 
      VYGIMNFFG MTTGIFVPM ITGFLKTNY KGFTGPTII 
      FMIGGIVFI GTGVVFVLF GSGEVQPWN KKEGEEEEQ 
      APAAAK
    CDS sequence
    • >TC034074 RA
      atggttaaa ttaaaagcg cctccatat cgggtatgg 
      atcgctgta atggttttt atgtcaact tttacaaac 
      tacatgctg agagcaaac atgtctgtg agtataatc 
      agtatggtt gacaaggcg aaaaaaaat cagacagca 
      gtttgcgca gctcctcct gacaattca accaaacct 
      cccccgaaa cccaaatct ggaagtaaa aaattcaaa 
      tggagtgag atcgacgaa ggctggatc ctcgctgcc 
      tatttctac ggctacttc ttcacagtg ctgcctggg 
      gggattata tcaaattat atcggccct tggcacact 
      atagtgtgg tcgagcctt ggcagtgcc gtcttaact 
      ggcttgacc cccatttgc gccacatct ggaggtgtg 
      ggtggggtt tgcgctaac cgatttttc atcggcttt 
      cttgggggg ccagtgtac cccgccgtc aacgattta 
      atcgcaaag tgggcgccg cccaaggaa aagggcaag 
      tttttggcg gctatgatg ggcaacacc ttggggacg 
      gtggtgact tggaatctg gtggcggcg gcctcctcg 
      gcattgggc tgggaatgg ggatttcac gttctggcc 
      atcttcatg ttggtttat tgcgctgtc tttggagta 
      attgtgagg gatagaccc gacatacat ccttgggtc 
      tccgaggat gaaaagaaa tatattgcg gaatctcaa 
      gaagggcgt cttaccacg aaaaagggc tttcccccg 
      taccacaaa atgatgtgt ttcaacttc ccgttctgg 
      gctttagtg atagcccag ttgggcaat ttgtggggc 
      ttgaacttg gtcctcact tacgccccc aaattcatg 
      gctggaacc ttaggcttc aatataaaa gcctcagcc 
      ggccttgct gcacttcca tacattgcg cgtcttatc 
      ggcagtaac atcttcgga ataatcggc gattacatg 
      cgtaaaaag gaaataatg tctgtgacc aaaattcga 
      aaattcttc ataattttc tcccatttt attccgggc 
      gccttgctg atagtaatg cgcgcgttc ggatgcatc 
      aaagagggc gtcattgcc atgatagtg ctaaaccaa 
      ggcttcaac ggcgcatgc gttgtcagc catttggtg 
      aacccgcaa gacctgtcc ccgaatttc gcaggagcc 
      gtttacgga atcatgaac ttcttcggc atgactacg 
      ggaatattc gtgcccatg attactgga ttcttgaaa 
      acgaattat aaaggattc acaggaccc acgataata 
      ttcatgatt ggaggaatt gtttttata ggcaccggg 
      gtggtgttt gtccttttt ggatccggg gaggtgcag 
      ccttggaac aagaaggaa ggcgaagag gaggagcaa 
      gcgccagca gcagcaaaa taa
    mRNA sequence
    • >TC034074 RA
      ctaaagatg ttttttatt ttatttaca aattaatca 
      acagtcctt gcttgcaac aagtcaaaa tttgtcttt 
      tttggcagt tgtgatgcc ctttgttca caatggtta 
      aattaaaag cgcctccat atcgggtat ggatcgctg 
      taatggttt ttatgtcaa cttttacaa actacatgc 
      tgagagcaa acatgtctg tgagtataa tcagtatgg 
      ttgacaagg cgaaaaaaa atcagacag cagtttgcg 
      cagctcctc ctgacaatt caaccaaac ctcccccga 
      aacccaaat ctggaagta aaaaattca aatggagtg 
      agatcgacg aaggctgga tcctcgctg cctatttct 
      acggctact tcttcacag tgctgcctg gggggatta 
      tatcaaatt atatcggcc cttggcaca ctatagtgt 
      ggtcgagcc ttggcagtg ccgtcttaa ctggcttga 
      cccccattt gcgccacat ctggaggtg tgggtgggg 
      tttgcgcta accgatttt tcatcggct ttcttgggg 
      ggccagtgt accccgccg tcaacgatt taatcgcaa 
      agtgggcgc cgcccaagg aaaagggca agtttttgg 
      cggctatga tgggcaaca ccttgggga cggtggtga 
      cttggaatc tggtggcgg cggcctcct cggcattgg 
      gctgggaat ggggatttc acgttctgg ccatcttca 
      tgttggttt attgcgctg tctttggag taattgtga 
      gggatagac ccgacatac atccttggg tctccgagg 
      atgaaaaga aatatattg cggaatctc aagaagggc 
      gtcttacca cgaaaaagg gctttcccc cgtaccaca 
      aaatgatgt gtttcaact tcccgttct gggctttag 
      tgatagccc agttgggca atttgtggg gcttgaact 
      tggtcctca cttacgccc ccaaattca tggctggaa 
      ccttaggct tcaatataa aagcctcag ccggccttg 
      ctgcacttc catacattg cgcgtctta tcggcagta 
      acatcttcg gaataatcg gcgattaca tgcgtaaaa 
      aggaaataa tgtctgtga ccaaaattc gaaaattct 
      tcataattt tctcccatt ttattccgg gcgccttgc 
      tgatagtaa tgcgcgcgt tcggatgca tcaaagagg 
      gcgtcattg ccatgatag tgctaaacc aaggcttca 
      acggcgcat gcgttgtca gccatttgg tgaacccgc 
      aagacctgt ccccgaatt tcgcaggag ccgtttacg 
      gaatcatga acttcttcg gcatgacta cgggaatat 
      tcgtgccca tgattactg gattcttga aaacgaatt 
      ataaaggat tcacaggac ccacgataa tattcatga 
      ttggaggaa ttgttttta taggcaccg gggtggtgt 
      ttgtccttt ttggatccg gggaggtgc agccttgga 
      acaagaagg aaggcgaag aggaggagc aagcgccag 
      cagcagcaa aataatgtt tatggcgtc atacttttg 
      gttatgtag tattttcca aatatcaga aaagtaact 
      cgaaataca cagtcctga ttatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY