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Gene information
General information
TC005716 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8547386..8551208
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005716
    iBeetle screen iB_10523

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005716 RA
      MGAPVLLYM YDLSEGWCR NLGPLCPVN 
      AVWHTSIVI YGKEYVFGS TGITFHDPG 
      KPDKVIELG ETDLPPFEF KVYVKELKY 
      TEWTGASYD PFKHNCNHF TDHIARYLG 
      MGPIPECVF TSVDRLRES PFYSKIVDW 
      VTKNGQNQQ K
    CDS sequence
    • >TC005716 RA
      atgggcgcc cccgtgtta ttgtacatg tacgacctg 
      tcagagggc tggtgcagg aatttgggg cccctgtgc 
      cctgtcaat gccgtttgg cacaccagt attgtaatc 
      tatggcaaa gaatacgtt tttgggagc accggaatt 
      acattccat gatcccgga aaacctgac aaagttatt 
      gaacttggg gagacggat ttacccccg tttgagttc 
      aaagtttac gttaaagaa ttgaaatat acggagtgg 
      acgggcgct tcgtatgac ccttttaag cacaactgc 
      aatcacttt actgaccac attgccagg tatttaggc 
      atggggcca attcccgaa tgtgtcttt acatcagtt 
      gataggtta cgggagtca cctttctac tcaaaaatc 
      gtggattgg gtcacgaaa aatggccaa aaccagcaa 
      aaataa
    mRNA sequence
    • >TC005716 RA
      acattaacg gaactatcg aggacggga acgttttat 
      ttacacgtc ctttcatat cctaacctt taggtatcc 
      gtaaataca aatacattt ttcagaatg agaatttac 
      aaaaattac tgaccccat ttattttaa taaattcat 
      aacagagaa atttggagc aaatgcgac aatttaaaa 
      aacaaagcc agaaaaata tcaacggaa tactgcaaa 
      tgcagctac ttaaaaaaa aaaacagaa aaaatggag 
      attatagag gcaaaacta aaaaaatta agctgaagc 
      aaagtgtga tctcataga aaaaaacgc gggatattg 
      agaacataa aaaattaag cttcttgaa ttgaaaata 
      aaaattaaa agtagcaaa gtaattgaa aattgtcaa 
      acattagca ttggcgttt tcttcaaaa aaatcatcg 
      ttctctaaa ttattatcc ttttcatta actttatag 
      tttatgttt tcttgcacg ttcatgttg atttgacaa 
      tttttgaga aaattaatt aaactcaaa taaagccaa 
      aaatttgtt tatgaaatc ggcccttag gctgagaca 
      tcgccaaga gttcgaggc agtagaagc acaatcgtg 
      tctatgtag taggttttg gagctttga aaacttgag 
      agacttgct gagctttag atttgaact aaaattttg 
      tttttctgt ggaagagct caacagcct ggtgattgt 
      aacattgtg cccttctgt ttttccacg cgccaattg 
      atttcacga ggttagtct aagagagtt agtttaatc 
      tgagtttgc gtgtcgatg agaatgtaa atttatgtg 
      tttcatgcg tccatttca tgtttggtt ctagtatcg 
      atcaccaaa gttgcgttt tgtaaataa ttgttgttg 
      tggaaagtc acttttact tgattggtt gaccctttc 
      gtgcacgcc attcggtta attggagct ttttcgtcc 
      cgggatcga cttttgtac agcctttgg ggtccagta 
      tttccccga acttatgat ttcttactg ttaaatagg 
      tataaaccg gtcgggagc gtgcaatgt gtaaaatag 
      actcactga aggtgaatt cctttgaaa tttgccata 
      atctagtaa ataagtctt agagaaatc gaaacgtat 
      cttaaccgt tgttgttac aatgtaaca attgtagag 
      atttcaaga ggtgcgttc gggtatacc gcaatttat 
      taatatccg atgataaac gtaatttac aaatttttc 
      ttacatgaa acttaggga gaatgttac gttgggccg 
      cgcgttatt cagttgtta catgaagca atgttttcg 
      gttcttgct tgtatttgt tggaaaatc gatgttaat 
      ttgtatttt tgaaaacta tttttctaa tggtctttc 
      gcgatacac ctgcggccg ttcaagcgg cgacgacac 
      gtgatattt tacgagaaa tttctgaaa tttgttcga 
      taagactcg actaagata ttaatgaaa tttgttttt 
      gtatggaaa ttcattagg tatgatgtg agtgatgat 
      tctattgtc aagtgacgt tgaggggtt atcgttgga 
      taaataaat gttacgttt cagctgtca aagctgtca 
      aaacaaaaa ttaaatatt gaatattta caattcagc 
      attgttaaa aatgtagat tttcaagca aacacgttg 
      aaaaatgca taaggcatg tgcctgcat tgtcttatg 
      ctgcaacac aaattaatt caaagtacg acaaaaata 
      gtaaggtta cgtagtatc tgtttattt atcttttag 
      aataagccc aaaatggat atatttgaa tgaaaggta 
      tcatcagag tccctaaaa atgggcgcc cccgtgtta 
      ttgtacatg tacgacctg tcagagggc tggtgcagg 
      aatttgggg cccctgtgc cctgtcaat gccgtttgg 
      cacaccagt attgtaatc tatggcaaa gaatacgtt 
      tttgggagc accggaatt acattccat gatcccgga 
      aaacctgac aaagttatt gaacttggg gagacggat 
      ttacccccg tttgagttc aaagtttac gttaaagaa 
      ttgaaatat acggagtgg acgggcgct tcgtatgac 
      ccttttaag cacaactgc aatcacttt actgaccac 
      attgccagg tatttaggc atggggcca attcccgaa 
      tgtgtcttt acatcagtt gataggtta cgggagtca 
      cctttctac tcaaaaatc gtggattgg gtcacgaaa 
      aatggccaa aaccagcaa aaataaaaa ttattaggt 
      acttccagc acggcttcc ttgattaat gaaggagcg 
      ccagctcgt atggtaact gtcctttaa cgtggcttc 
      ctctcattg gtcaaggaa gctgcgctt cataagcca 
      gaaatgttc aaggaatta taaaataaa tttttgcca 
      tttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY