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Gene information
General information
TC008020 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3999166..4001357
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008020
    iBeetle screen iB_10520

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content not present - Number of animals showing the phenotype: 6 (13 dpi)
    Days post injection



    abdominal odoriferous glands partially content darker - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    abdominal odoriferous glands content necrotic - Number of animals showing the phenotype: 6 (13 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008020 RA
      MISSGDINI QNFQSLIEN YEAALKEVT GDLDDVQAE 
      QKALKRALH ENSQLRDEL GRVYVDITK CEVEHTYKE 
      SDLVENLKK QLHTVIEEK EQAVKLWQN AASTVELLE 
      SELRIFHEN TEGFIPKNQ LTQLKNSYE AKLNELQDT 
      VVRTKVKLE ETINLKSKE LQIKDKEII GSLQSQDNT 
      FKIIKNLED EILNLQQRL NDSNGNKEK LEKDVRLKN 
      EEIDALKLK VKECKAKVS EALQVVEAA LNEKDAALL 
      REAEAKEET ARFSQTLTE FIEETDLKI RNIQSDHET 
      RVEQMRQAL EASQELVRV KEMEIETYV KKCAILENQ 
      IEDFRKNRT YVDDGGTSK ILVLEKNLE NTFQKLVSA 
      FFAFLTPIF SYFQRSRIF SSSLKWRT
    • >TC008020 RB
      MISSGDINI QNFQSLIEN YEAALKEVT GDLDDVQAE 
      QKALKRALH ENSQLRDEL GRVYVDITK CEVEHTYKE 
      SDLVENLKK QLHTVIEEK EQAVKLWQN AASTVELLE 
      SELRIFHEN TEGFIPKNQ LTQLKNSYE AKLNELQDT 
      VVRTKVKLE ETINLKSKE LQIKDKEII GSLQSQDNT 
      FKIIKNLED EILNLQQRL NDSNGNKEK LEKDVRLKN 
      EEIDALKLK VKECKAKVS EALQVVEAA LNEKDAALL 
      REAEAKEET ARFSQTLTE FIEETDLKI RNIQSDHET 
      RVEQMRQAL EASQELVRV KEMEIETYV KKCAILENQ 
      IEDFRKNRT YVDDGGTSK ILVLEKNLE NTFQKLLLS 
      EKQNIQLKS EMEDVKNDL DQMSHYYER DINAREAEK 
      TALKNEIKR LKAELEQSF LRVSESTAT INDLNEKIS 
      SMEINFKEQ IKETKNSFQ QTCQERQEM ETFHQQLVQ 
      ELQEQLISQ TEINRKWRT ETKNITQQL EKIITELKS 
      ANNKMKLEN KVLNERLKD AH
    CDS sequence
    • >TC008020 RA
      atgataagt tcaggggat attaatatt caaaatttc 
      cagtcactt attgaaaat tatgaggct gctctcaag 
      gaggtgacc ggggatttg gacgacgtc caagccgaa 
      caaaaagca ctcaagaga gccctccac gaaaatagt 
      caattgaga gatgaatta gggcgagtt tacgtcgat 
      ataactaaa tgtgaagtt gaacatacg tacaaagag 
      agcgatttg gttgaaaat ttgaagaaa caactgcac 
      actgtaatt gaagaaaaa gaacaagcg gtcaaatta 
      tggcaaaat gccgcatct actgttgaa ctccttgag 
      tcagagctg cgaattttt cacgaaaac accgaagga 
      tttattccg aaaaaccaa ctcacacaa ttgaaaaat 
      tcgtacgaa gcgaaatta aatgaattg caagacaca 
      gtcgttcgg acgaaagtc aaattagag gaaacaatt 
      aatttaaag tcgaaggaa ttgcaaatt aaagacaag 
      gaaattatt ggaagtctc caaagtcag gacaacaca 
      tttaaaata ataaaaaat ctcgaagac gaaattctg 
      aacctccaa caaagactc aacgactcg aacggaaat 
      aaggagaag ttggaaaaa gacgtccgt ctcaaaaat 
      gaagaaatc gacgctttg aaactcaaa gtcaaagaa 
      tgtaaagcc aaagtttcg gaagctctt caagttgtt 
      gaagcggcg cttaacgaa aaagacgcg gcgcttttg 
      cgcgaagct gaagccaaa gaagaaaca gcgcgattt 
      tcccaaact ttgaccgaa tttatcgaa gaaaccgac 
      ttgaaaatc agaaatata caaagcgac cacgagaca 
      agagtggaa cagatgaga caagcactg gaggcgagt 
      caggagttg gtgagagtg aaggagatg gaaattgaa 
      acttatgtg aaaaaatgc gctatattg gagaaccaa 
      attgaggat tttcgcaaa aatcggact tacgtcgac 
      gatggaggg acttctaaa atcctagtt cttgagaaa 
      aatctcgaa aacacgttt cagaaattg gtgagcgct 
      ttctttgca tttctaaca ccgattttt agttacttt 
      cagagaagc agaatattc agctcaagt ctgaaatgg 
      aggacgtaa
    • >TC008020 RB
      atgataagt tcaggggat attaatatt caaaatttc 
      cagtcactt attgaaaat tatgaggct gctctcaag 
      gaggtgacc ggggatttg gacgacgtc caagccgaa 
      caaaaagca ctcaagaga gccctccac gaaaatagt 
      caattgaga gatgaatta gggcgagtt tacgtcgat 
      ataactaaa tgtgaagtt gaacatacg tacaaagag 
      agcgatttg gttgaaaat ttgaagaaa caactgcac 
      actgtaatt gaagaaaaa gaacaagcg gtcaaatta 
      tggcaaaat gccgcatct actgttgaa ctccttgag 
      tcagagctg cgaattttt cacgaaaac accgaagga 
      tttattccg aaaaaccaa ctcacacaa ttgaaaaat 
      tcgtacgaa gcgaaatta aatgaattg caagacaca 
      gtcgttcgg acgaaagtc aaattagag gaaacaatt 
      aatttaaag tcgaaggaa ttgcaaatt aaagacaag 
      gaaattatt ggaagtctc caaagtcag gacaacaca 
      tttaaaata ataaaaaat ctcgaagac gaaattctg 
      aacctccaa caaagactc aacgactcg aacggaaat 
      aaggagaag ttggaaaaa gacgtccgt ctcaaaaat 
      gaagaaatc gacgctttg aaactcaaa gtcaaagaa 
      tgtaaagcc aaagtttcg gaagctctt caagttgtt 
      gaagcggcg cttaacgaa aaagacgcg gcgcttttg 
      cgcgaagct gaagccaaa gaagaaaca gcgcgattt 
      tcccaaact ttgaccgaa tttatcgaa gaaaccgac 
      ttgaaaatc agaaatata caaagcgac cacgagaca 
      agagtggaa cagatgaga caagcactg gaggcgagt 
      caggagttg gtgagagtg aaggagatg gaaattgaa 
      acttatgtg aaaaaatgc gctatattg gagaaccaa 
      attgaggat tttcgcaaa aatcggact tacgtcgac 
      gatggaggg acttctaaa atcctagtt cttgagaaa 
      aatctcgaa aacacgttt cagaaattg ttactttca 
      gagaagcag aatattcag ctcaagtct gaaatggag 
      gacgtaaaa aacgacttg gaccaaatg tcccactat 
      tacgagcgg gacattaac gcgagagaa gccgagaaa 
      acggcccta aaaaacgaa ataaaacga ctcaaagct 
      gaactcgag caaagcttt ctgagggtg tccgaaagc 
      acggccacc ataaacgat ttgaatgaa aaaatctcg 
      tccatggag ataaacttc aaagaacag attaaagaa 
      acgaagaat tccttccag cagacgtgt caagagcgc 
      caagaaatg gagactttt caccaacaa ttagtccaa 
      gaattgcaa gaacagttg atttcgcag acggaaatc 
      aacaggaaa tggcggacc gagacgaaa aacatcaca 
      caacaattg gaaaaaatc attacggaa ttaaaatca 
      gcgaacaac aaaatgaaa ctcgagaat aaagtcctt 
      aacgagcgt ttgaaggac gcacat
    mRNA sequence
    • >TC008020 RA
      cggcgcttc attcgaaat tagcgccga cacttatcg 
      atcgttcca cctgtaaaa ttagaccga ctccccgta 
      aacacaaac aaatcgcca cattattta cacccacat 
      ttggtcaaa attcttcgt tatttgcaa ataatcgca 
      caattattg aaataaacg tgaataatc ctcatttga 
      ctgttttaa tttgtgctc gtttggtgt gaccttcgg 
      cagatgtct ctgtcacat tttcaaaat ttgaaatcc 
      ttcaaaaaa tgtcatcgt aatggtttt ttggagtta 
      ttattatga taagttcag gggatatta atattcaaa 
      atttccagt cacttattg aaaattatg aggctgctc 
      tcaaggagg tgaccgggg atttggacg acgtccaag 
      ccgaacaaa aagcactca agagagccc tccacgaaa 
      atagtcaat tgagagatg aattagggc gagtttacg 
      tcgatataa ctaaatgtg aagttgaac atacgtaca 
      aagagagcg atttggttg aaaatttga agaaacaac 
      tgcacactg taattgaag aaaaagaac aagcggtca 
      aattatggc aaaatgccg catctactg ttgaactcc 
      ttgagtcag agctgcgaa tttttcacg aaaacaccg 
      aaggattta ttccgaaaa accaactca cacaattga 
      aaaattcgt acgaagcga aattaaatg aattgcaag 
      acacagtcg ttcggacga aagtcaaat tagaggaaa 
      caattaatt taaagtcga aggaattgc aaattaaag 
      acaaggaaa ttattggaa gtctccaaa gtcaggaca 
      acacattta aaataataa aaaatctcg aagacgaaa 
      ttctgaacc tccaacaaa gactcaacg actcgaacg 
      gaaataagg agaagttgg aaaaagacg tccgtctca 
      aaaatgaag aaatcgacg ctttgaaac tcaaagtca 
      aagaatgta aagccaaag tttcggaag ctcttcaag 
      ttgttgaag cggcgctta acgaaaaag acgcggcgc 
      ttttgcgcg aagctgaag ccaaagaag aaacagcgc 
      gattttccc aaactttga ccgaattta tcgaagaaa 
      ccgacttga aaatcagaa atatacaaa gcgaccacg 
      agacaagag tggaacaga tgagacaag cactggagg 
      cgagtcagg agttggtga gagtgaagg agatggaaa 
      ttgaaactt atgtgaaaa aatgcgcta tattggaga 
      accaaattg aggattttc gcaaaaatc ggacttacg 
      tcgacgatg gagggactt ctaaaatcc tagttcttg 
      agaaaaatc tcgaaaaca cgtttcaga aattggtga 
      gcgctttct ttgcatttc taacaccga tttttagtt 
      actttcaga gaagcagaa tattcagct caagtctga 
      aatggagga cgtaaaaaa cgacttgga ccaaatgtc 
      ccactatta cgagcggga cattaacgc gagagaagc 
      cgagaaaac ggccctaaa aaacgaaat aaaacgact 
      caaagctga actcgagca aagctttct gagggtgtc 
      cgaaagcac ggccaccat aaacgattt gaatgaaaa 
      aatctcgtc catggagat aaacttcaa agaacagat 
      taaagaaac gaagaattc cttccagca gacgtgtca 
      agagcgcca agaaatgga gacttttca ccaacaatt 
      agtccaaga attgcaaga acagttgat ttcgcagac 
      ggaaatcaa caggaaatg gcggaccga gacgaaaaa 
      catcacaca acaattgga aaaaatcat tacggaatt 
      aaaatcagc gaacaacaa aatgaaact cgagaataa 
      agtccttaa cgagcgttt gaaggacgc acatgtgaa 
      aatgttcga gtatcgcaa attccttga aatgatttc 
      gcacgacgt cacgaaaat tacgacagt gactttgcc 
      cgaaataaa agagtgtgc taggt
    • >TC008020 RB
      cggcgcttc attcgaaat tagcgccga cacttatcg 
      atcgttcca cctgtaaaa ttagaccga ctccccgta 
      aacacaaac aaatcgcca cattattta cacccacat 
      ttggtcaaa attcttcgt tatttgcaa ataatcgca 
      caattattg aaataaacg tgaataatc ctcatttga 
      ctgttttaa tttgtgctc gtttggtgt gaccttcgg 
      cagatgtct ctgtcacat tttcaaaat ttgaaatcc 
      ttcaaaaaa tgtcatcgt aatggtttt ttggagtta 
      ttattatga taagttcag gggatatta atattcaaa 
      atttccagt cacttattg aaaattatg aggctgctc 
      tcaaggagg tgaccgggg atttggacg acgtccaag 
      ccgaacaaa aagcactca agagagccc tccacgaaa 
      atagtcaat tgagagatg aattagggc gagtttacg 
      tcgatataa ctaaatgtg aagttgaac atacgtaca 
      aagagagcg atttggttg aaaatttga agaaacaac 
      tgcacactg taattgaag aaaaagaac aagcggtca 
      aattatggc aaaatgccg catctactg ttgaactcc 
      ttgagtcag agctgcgaa tttttcacg aaaacaccg 
      aaggattta ttccgaaaa accaactca cacaattga 
      aaaattcgt acgaagcga aattaaatg aattgcaag 
      acacagtcg ttcggacga aagtcaaat tagaggaaa 
      caattaatt taaagtcga aggaattgc aaattaaag 
      acaaggaaa ttattggaa gtctccaaa gtcaggaca 
      acacattta aaataataa aaaatctcg aagacgaaa 
      ttctgaacc tccaacaaa gactcaacg actcgaacg 
      gaaataagg agaagttgg aaaaagacg tccgtctca 
      aaaatgaag aaatcgacg ctttgaaac tcaaagtca 
      aagaatgta aagccaaag tttcggaag ctcttcaag 
      ttgttgaag cggcgctta acgaaaaag acgcggcgc 
      ttttgcgcg aagctgaag ccaaagaag aaacagcgc 
      gattttccc aaactttga ccgaattta tcgaagaaa 
      ccgacttga aaatcagaa atatacaaa gcgaccacg 
      agacaagag tggaacaga tgagacaag cactggagg 
      cgagtcagg agttggtga gagtgaagg agatggaaa 
      ttgaaactt atgtgaaaa aatgcgcta tattggaga 
      accaaattg aggattttc gcaaaaatc ggacttacg 
      tcgacgatg gagggactt ctaaaatcc tagttcttg 
      agaaaaatc tcgaaaaca cgtttcaga aattgttac 
      tttcagaga agcagaata ttcagctca agtctgaaa 
      tggaggacg taaaaaacg acttggacc aaatgtccc 
      actattacg agcgggaca ttaacgcga gagaagccg 
      agaaaacgg ccctaaaaa acgaaataa aacgactca 
      aagctgaac tcgagcaaa gctttctga gggtgtccg 
      aaagcacgg ccaccataa acgatttga atgaaaaaa 
      tctcgtcca tggagataa acttcaaag aacagatta 
      aagaaacga agaattcct tccagcaga cgtgtcaag 
      agcgccaag aaatggaga cttttcacc aacaattag 
      tccaagaat tgcaagaac agttgattt cgcagacgg 
      aaatcaaca ggaaatggc ggaccgaga cgaaaaaca 
      tcacacaac aattggaaa aaatcatta cggaattaa 
      aatcagcga acaacaaaa tgaaactcg agaataaag 
      tccttaacg agcgtttga aggacgcac attgacttt 
      gcccgaaat aaaagagtg tgctaggt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY