Status
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Gene information
General information
TC009386 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1385345..1388594
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009386
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10513

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009386 RA
      MDPQKLQAK NVIISQWNL VNQLKTTLS KFTNEKTVE 
      NALNQEFTK FLQRIQPTI NGIDLTKYF NALSLPIFK 
      NSEQISETT VAKNSVKEM KKVVPKWKE NSVTTKESV 
      ASRTHQILD LLASAKSHQ TILNRTEAL NAHLNKYPE 
      AKHFAVKSN AIPLLLKIK TGNANNDTI SGAVNETLA 
      LLGYAGPVS GKGVRILSI DGGGVRGIL VIEMLKKLE 
      ELTGKRISE MFDLICGVS TGAIIASLV GVKRYTLDE 
      ISEIYKNLS TQIFTQSAL KGTSSLVWS HSYYDTARW 
      EKLLQEQIG NQTLISTAR SSHCPRLCV LSAVVNQSR 
      LSAYVFRNY SLPCRVQSQ YLGSHKHLI WEAVRASAA 
      APTYFEEFK LENMLHQDG GILFNNPTA VAIHEARLL 
      WPEAPIQCV LSFGTGRTI PLPVDPNTQ KAVRNSSWK 
      SKFYAIIES ATDTEGVHT MLSDLLPPS IYYRFNPYL 
      TEMLDIAEI DPKKVEQLL RDAVMYLRR NEDKFHEAA 
      RVLLQEKSL SQKAIDWLS LQQEIRGFR RVPQTLLTA
    CDS sequence
    • >TC009386 RA
      atggacccc caaaaactg caagcaaaa aacgtgatc 
      atttcccag tggaattta gtcaatcaa ctcaaaacc 
      acactatca aaattcaca aacgagaag actgtggaa 
      aatgcctta aatcaagaa tttacaaag tttttgcaa 
      cgaatccaa ccaacaatc aatggaatc gacctgaca 
      aaatacttt aatgcttta tcattgcca attttcaaa 
      aactctgaa caaataagt gagaccaca gttgctaaa 
      aacagtgtg aaagaaatg aaaaaagtt gtgccaaaa 
      tggaaagaa aatagcgtt accacaaag gaatcggta 
      gcctctcga acacatcaa attttagat ctgttggca 
      agtgccaag tcacaccaa acgattttg aatcgaacg 
      gaagcatta aatgcgcac ctgaataaa taccccgaa 
      gccaaacac tttgcagtt aagagcaac gcaattccc 
      ttattatta aaaataaaa accggaaat gcgaataac 
      gacacaatt agtggggcc gtcaacgaa acactggcc 
      ctactgggg tacgcaggc cctgtctct ggaaaaggg 
      gtccgcatt ttatcaatc gacgggggc ggagtccga 
      ggaatcctc gtaatcgaa atgttgaaa aaactggaa 
      gagttaacc ggaaaacgg atttccgag atgtttgac 
      ctcatttgt ggcgtcagc actggggct ataattgcc 
      agtttagtc ggtgttaaa cgctacact ttggatgaa 
      atttccgaa atttacaag aatttgagt actcaaatt 
      tttactcag tctgcgctc aagggcaca agtagcctg 
      gtgtggagc cactcttat tacgacact gcgcggtgg 
      gagaagctc cttcaggag caaattggc aatcagacg 
      cttatttca acggccagg agctcgcac tgccccagg 
      ctttgcgtg ttatcagct gttgtaaat caatcaaga 
      ctatcagct tacgttttt cgcaactat tctttacca 
      tgtcgtgtg cagtcccaa tatttggga agccataaa 
      cacttaatc tgggaagcg gtgagggca tcggccgca 
      gcgccaact tacttcgag gaattcaaa ttggagaac 
      atgctgcac caggacggc ggtatttta ttcaacaac 
      ccaacggcg gtggcaatc cacgaagcc agactcctc 
      tggccggaa gcccccatc cagtgcgtg ctgtcgttt 
      ggcaccggc cgtaccatc cccttgccc gtcgacccc 
      aacacccag aaagcggtc cggaattcg tcgtggaaa 
      agcaagttc tatgccatt attgagagc gcaacggac 
      acagaaggg gtgcacaca atgctctca gatctgctg 
      cccccgagt atttactac cggttcaat ccgtatctg 
      accgaaatg ttggatatt gcggaaatt gatccgaaa 
      aaagtggag cagttatta agggatgct gtcatgtat 
      ttgaggcgg aacgaggat aagtttcac gaagctgca 
      agggttttg ctccaggag aaatcgcta agtcagaaa 
      gccattgat tggttgagt ctgcagcag gagataagg 
      ggcttcaga cgggtgccg caaacgtta ctcacggcg 
      tag
    mRNA sequence
    • >TC009386 RA
      tatgaacac agtcaaaaa ttgttttga tcgcgttga 
      tgtcgacgt acttgacaa taaattcaa accccttcc 
      atcgccctg tttatcaac atttgctta gtatttatc 
      gaacagcga cgaggaacg aattatcaa tctgtggat 
      ttttctagt acagtgcga gtgcggaaa atacttgac 
      gacattaaa caacccacg caattgtcc gcgaccagt 
      gtcacagct aatttaaaa cactattcg agaacacgg 
      acaggaatt gtattttta aattcggga gtgaatttg 
      ttgaaaaat gtcgatttc gaagaacct aagtcgcta 
      taaaaattg tgttgggaa aattcccgc aaggcagct 
      aaactgtga tagttccaa cgcaatgga cccccaaaa 
      actgcaagc aaaaaacgt gatcatttc ccagtggaa 
      tttagtcaa tcaactcaa aaccacact atcaaaatt 
      cacaaacga gaagactgt ggaaaatgc cttaaatca 
      agaatttac aaagttttt gcaacgaat ccaaccaac 
      aatcaatgg aatcgacct gacaaaata ctttaatgc 
      tttatcatt gccaatttt caaaaactc tgaacaaat 
      aagtgagac cacagttgc taaaaacag tgtgaaaga 
      aatgaaaaa agttgtgcc aaaatggaa agaaaatag 
      cgttaccac aaaggaatc ggtagcctc tcgaacaca 
      tcaaatttt agatctgtt ggcaagtgc caagtcaca 
      ccaaacgat tttgaatcg aacggaagc attaaatgc 
      gcacctgaa taaataccc cgaagccaa acactttgc 
      agttaagag caacgcaat tcccttatt attaaaaat 
      aaaaaccgg aaatgcgaa taacgacac aattagtgg 
      ggccgtcaa cgaaacact ggccctact ggggtacgc 
      aggccctgt ctctggaaa aggggtccg cattttatc 
      aatcgacgg gggcggagt ccgaggaat cctcgtaat 
      cgaaatgtt gaaaaaact ggaagagtt aaccggaaa 
      acggatttc cgagatgtt tgacctcat ttgtggcgt 
      cagcactgg ggctataat tgccagttt agtcggtgt 
      taaacgcta cactttgga tgaaatttc cgaaattta 
      caagaattt gagtactca aatttttac tcagtctgc 
      gctcaaggg cacaagtag cctggtgtg gagccactc 
      ttattacga cactgcgcg gtgggagaa gctccttca 
      ggagcaaat tggcaatca gacgcttat ttcaacggc 
      caggagctc gcactgccc caggctttg cgtgttatc 
      agctgttgt aaatcaatc aagactatc agcttacgt 
      ttttcgcaa ctattcttt accatgtcg tgtgcagtc 
      ccaatattt gggaagcca taaacactt aatctggga 
      agcggtgag ggcatcggc cgcagcgcc aacttactt 
      cgaggaatt caaattgga gaacatgct gcaccagga 
      cggcggtat tttattcaa caacccaac ggcggtggc 
      aatccacga agccagact cctctggcc ggaagcccc 
      catccagtg cgtgctgtc gtttggcac cggccgtac 
      catcccctt gcccgtcga ccccaacac ccagaaagc 
      ggtccggaa ttcgtcgtg gaaaagcaa gttctatgc 
      cattattga gagcgcaac ggacacaga aggggtgca 
      cacaatgct ctcagatct gctgccccc gagtattta 
      ctaccggtt caatccgta tctgaccga aatgttgga 
      tattgcgga aattgatcc gaaaaaagt ggagcagtt 
      attaaggga tgctgtcat gtatttgag gcggaacga 
      ggataagtt tcacgaagc tgcaagggt tttgctcca 
      ggagaaatc gctaagtca gaaagccat tgattggtt 
      gagtctgca gcaggagat aaggggctt cagacgggt 
      gccgcaaac gttactcac ggcgtagca caataagag 
      tattatcaa ttattttag attagaaaa ttaggtttt 
      gtattcgat ttgtcttaa tagatttac aacaattcg 
      gttgcaatt tgcgacttc taccattag tgatagaaa 
      tggttgtga taattatta agttaagga gaactgttt 
      ttattttag acaatttga aataaattt ttttagtaa 
      ca
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