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Gene information
General information
TC010061 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9612117..9616955
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010061
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10511

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva partially posterioreverted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010061 RA
      MILQEKTRI DAEESHKSP NESEYDSAG SYSDEEEDK 
      ARSRNSREH TDSETEGSV GLERESGDG QESLPLRKV 
      DDDEDKKNP QYIPKRGTF YEHDDRTAD DEQTADNVE 
      LEKEKEQKK KVWQDKKER WSHDRFNDS EQAPKSRAE 
      LVAIYGYDI RNEEGPPRA RRRRRYGRG PNKYTRNWE 
      DEDAYNKPP PLIRKKKPL RKPNQGEDF PPLNRSSNQ 
      EDPQDSRDY EEKTDQYQA ETNGNTKNS VCESPQSPP 
      VPPQESNRM QQRVGSGRV VKPKREIKD SDYRGFTTK 
      TRQVRNMKT DQKVVPKSS KKDDFIQSQ NFTNKNNNI 
      QELEKDLSK LNVQDGVHK GGKHGGQRQ GSVPPRLQS 
      EQKGSKRYS SIRQRSLPE SATPPFSQH SNYYSNEFN 
      QAPNQVTIH QNAQIHPST TQVPQIAPP PLPPIPQVP 
      VTAAPILQA PQFAPPFAQ APPFLQPAP PPFIPQQTP 
      QIINYVPGQ PQFSQGYQG YQQQFNPVT QPELYQPQT 
      GITYYSTDQ QVTQRAAPL KRPKAAIPI VAPPPIDLK 
      GKVKEEDAL ATEGYVSTA NDGQTSDSG SAQESNLQD 
      CGYARRYSL Y
    • >TC010061 RB
      MILQEKTRI DAEESHKSP NESEYDSAG SYSDEEEDK 
      ARSRNSREH TDSETEGSV GLERESGDG QESLPLRKV 
      DDDEDKKNP QYIPKRGTF YEHDDRTAD DEQTADNVE 
      LEKEKEQKK KVWQDKKER WSHDRFNDS EQAPKSRAE 
      LVAIYGYDI RNEEGPPRA RRRRRYGRG PNKYTRNWE 
      DEDAYNKPP PLIRKKKPL RKPNQGEDF PPLNRSSNQ 
      EDPQDSRDY EEKTDQYQA ETNGNTKNS VCESPQSPP 
      VPPQESNRM QQRVGSGRV VKPKREIKD SDYRGFTTK 
      TRQVRNMKT DQKVVPKSS KKDDFIQSQ NFTNKNNNI 
      QELEKDLSK LNVQDGVHK GGKHGGQRQ GSVPPRLQS 
      EQKGSKRYS SIRQRSLPE SATPPFSQH SNYYSNEFN 
      QAPNQVTIH QNAQIHPST TQVPQIAPP PLPPIPQVP 
      VTAAPILQA PQFAPPFAQ APPFLQPAP PPFIPQQTP 
      QIINYVPGQ PQFSQGYQG YQQQFNPVT QPELYQPQT 
      GITYYSTDQ QVTQRAAPL KRPKAAIPI VAPPPIDLK 
      GKVKEEDAL ATEGYVSTA NDGQTSDSG SAQESNLQV 
      QQ
    CDS sequence
    • >TC010061 RA
      atgattttg caggaaaag acacgaatt gacgctgag 
      gagagccac aagtcccct aacgagtcg gagtatgac 
      agtgcaggg agttacagt gatgaagag gaggataaa 
      gcgaggtcg aggaattcc agggagcac actgattcg 
      gaaactgaa ggcagtgta gggctggag cgggaatct 
      ggcgacgga caggagagt ttgcctctc aggaaggtg 
      gacgatgat gaggacaag aaaaacccc cagtacata 
      cccaaaagg ggcactttt tacgaacac gatgaccgg 
      acagctgat gacgagcaa acagctgat aatgtagaa 
      ttggagaag gaaaaagaa cagaagaaa aaagtatgg 
      caggataag aaggaaagg tggagtcat gatcgtttt 
      aacgactct gagcaagcc cccaagtcc agggctgaa 
      cttgtggcc atctatggg tacgatatt aggaacgag 
      gaaggtccc cctcgagct cgaaggcgc agacgatac 
      ggacgtggc cctaacaag tatacaaga aactgggaa 
      gacgaagat gcctacaac aaaccgcca cctcttatt 
      cgtaaaaag aaacccctc agaaagccc aatcaaggc 
      gaagacttt ccgcctcta aaccgctca agtaatcaa 
      gaagacccg caggatagc agagattac gaagagaaa 
      acagaccag taccaagct gaaacaaac ggtaacacg 
      aaaaattca gtatgcgag tctccgcag tctccgcca 
      gtcccacca caagaatcc aacaggatg cagcaacgc 
      gtcggttct ggccgtgtg gttaaacca aaacgcgaa 
      atcaaggat tcagattat aggggtttt actacaaaa 
      acacgtcaa gttaggaat atgaaaact gatcaaaaa 
      gtcgtcccg aaatcctcg aaaaaagac gattttatc 
      cagtcgcaa aattttaca aataaaaat aacaacata 
      caagagcta gaaaaggat ttatcaaaa ttgaacgtt 
      caggatggg gtgcataaa gggggtaaa cacggggga 
      cagagacaa ggcagcgtc ccaccaaga ctccaatcg 
      gaacaaaag ggctccaaa cggtacagt tccatcaga 
      cagaggtcg cttccagag tcggcaacg ccgccgttc 
      tcgcagcac tcgaattac tactcaaat gaattcaac 
      caagcaccg aatcaagtg accattcat caaaacgcg 
      cagattcat ccgtccacg actcaagtc ccccaaatc 
      gcgccgccc cctctgccc cccatcccg caggttccc 
      gtcacggca gcccccatc ctgcaggcg ccccaattc 
      gcgcctccg ttcgcccaa gctcccccg tttttacaa 
      ccggcgccc cctccattc atccctcag caaaccccc 
      caaattatc aactacgtg ccgggtcaa ccgcagttc 
      tctcagggg tatcagggc taccagcag cagttcaat 
      cctgtgact cagcctgag ctgtaccag ccccagacg 
      gggatcacg tactactcg actgaccaa caggtgacg 
      cagagggcg gcaccgctc aagcgtccg aaagctgcg 
      atcccgatt gtggcgcct ccgccgatc gatctgaaa 
      ggtaaggtc aaggaggag gacgcgttg gcaaccgag 
      gggtatgtg tcgactgcc aacgatgga caaacaagc 
      gacagtggc agcgctcag gaatcaaat ttacaggat 
      tgtggttat gccagaaga tatagtctc tattaa
    • >TC010061 RB
      atgattttg caggaaaag acacgaatt gacgctgag 
      gagagccac aagtcccct aacgagtcg gagtatgac 
      agtgcaggg agttacagt gatgaagag gaggataaa 
      gcgaggtcg aggaattcc agggagcac actgattcg 
      gaaactgaa ggcagtgta gggctggag cgggaatct 
      ggcgacgga caggagagt ttgcctctc aggaaggtg 
      gacgatgat gaggacaag aaaaacccc cagtacata 
      cccaaaagg ggcactttt tacgaacac gatgaccgg 
      acagctgat gacgagcaa acagctgat aatgtagaa 
      ttggagaag gaaaaagaa cagaagaaa aaagtatgg 
      caggataag aaggaaagg tggagtcat gatcgtttt 
      aacgactct gagcaagcc cccaagtcc agggctgaa 
      cttgtggcc atctatggg tacgatatt aggaacgag 
      gaaggtccc cctcgagct cgaaggcgc agacgatac 
      ggacgtggc cctaacaag tatacaaga aactgggaa 
      gacgaagat gcctacaac aaaccgcca cctcttatt 
      cgtaaaaag aaacccctc agaaagccc aatcaaggc 
      gaagacttt ccgcctcta aaccgctca agtaatcaa 
      gaagacccg caggatagc agagattac gaagagaaa 
      acagaccag taccaagct gaaacaaac ggtaacacg 
      aaaaattca gtatgcgag tctccgcag tctccgcca 
      gtcccacca caagaatcc aacaggatg cagcaacgc 
      gtcggttct ggccgtgtg gttaaacca aaacgcgaa 
      atcaaggat tcagattat aggggtttt actacaaaa 
      acacgtcaa gttaggaat atgaaaact gatcaaaaa 
      gtcgtcccg aaatcctcg aaaaaagac gattttatc 
      cagtcgcaa aattttaca aataaaaat aacaacata 
      caagagcta gaaaaggat ttatcaaaa ttgaacgtt 
      caggatggg gtgcataaa gggggtaaa cacggggga 
      cagagacaa ggcagcgtc ccaccaaga ctccaatcg 
      gaacaaaag ggctccaaa cggtacagt tccatcaga 
      cagaggtcg cttccagag tcggcaacg ccgccgttc 
      tcgcagcac tcgaattac tactcaaat gaattcaac 
      caagcaccg aatcaagtg accattcat caaaacgcg 
      cagattcat ccgtccacg actcaagtc ccccaaatc 
      gcgccgccc cctctgccc cccatcccg caggttccc 
      gtcacggca gcccccatc ctgcaggcg ccccaattc 
      gcgcctccg ttcgcccaa gctcccccg tttttacaa 
      ccggcgccc cctccattc atccctcag caaaccccc 
      caaattatc aactacgtg ccgggtcaa ccgcagttc 
      tctcagggg tatcagggc taccagcag cagttcaat 
      cctgtgact cagcctgag ctgtaccag ccccagacg 
      gggatcacg tactactcg actgaccaa caggtgacg 
      cagagggcg gcaccgctc aagcgtccg aaagctgcg 
      atcccgatt gtggcgcct ccgccgatc gatctgaaa 
      ggtaaggtc aaggaggag gacgcgttg gcaaccgag 
      gggtatgtg tcgactgcc aacgatgga caaacaagc 
      gacagtggc agcgctcag gaatcaaat ttacaggtg 
      caacaatga
    mRNA sequence
    • >TC010061 RA
      ctagttggc accaacttg tttctctct gtttctttt 
      cctttcacg tcgttccat tgagcatga aaaggacca 
      gaaacgaga cgtaagtct tgaatttgt cactggcca 
      tcgaatctt gccaatccg gccaagata aagatattt 
      gtaaaacaa caaaaagat ggaagaaac cgttgctgt 
      gccagcgac agcccccca aacggtgga aaacatatc 
      acaggtgag cccaattgc tccttacgt tgctccttc 
      gttccggaa aagagaaag atttgagaa aaaattaac 
      aaaccaggt cacaagttc agaacgaag tggagcaca 
      accgaggcg atctcgcgt gaattcttc tcagtttaa 
      gaatgattt tgcaggaaa agacacgaa ttgacgctg 
      aggagagcc acaagtccc ctaacgagt cggagtatg 
      acagtgcag ggagttaca gtgatgaag aggaggata 
      aagcgaggt cgaggaatt ccagggagc acactgatt 
      cggaaactg aaggcagtg tagggctgg agcgggaat 
      ctggcgacg gacaggaga gtttgcctc tcaggaagg 
      tggacgatg atgaggaca agaaaaacc cccagtaca 
      tacccaaaa ggggcactt tttacgaac acgatgacc 
      ggacagctg atgacgagc aaacagctg ataatgtag 
      aattggaga aggaaaaag aacagaaga aaaaagtat 
      ggcaggata agaaggaaa ggtggagtc atgatcgtt 
      ttaacgact ctgagcaag cccccaagt ccagggctg 
      aacttgtgg ccatctatg ggtacgata ttaggaacg 
      aggaaggtc cccctcgag ctcgaaggc gcagacgat 
      acggacgtg gccctaaca agtatacaa gaaactggg 
      aagacgaag atgcctaca acaaaccgc cacctctta 
      ttcgtaaaa agaaacccc tcagaaagc ccaatcaag 
      gcgaagact ttccgcctc taaaccgct caagtaatc 
      aagaagacc cgcaggata gcagagatt acgaagaga 
      aaacagacc agtaccaag ctgaaacaa acggtaaca 
      cgaaaaatt cagtatgcg agtctccgc agtctccgc 
      cagtcccac cacaagaat ccaacagga tgcagcaac 
      gcgtcggtt ctggccgtg tggttaaac caaaacgcg 
      aaatcaagg attcagatt ataggggtt ttactacaa 
      aaacacgtc aagttagga atatgaaaa ctgatcaaa 
      aagtcgtcc cgaaatcct cgaaaaaag acgatttta 
      tccagtcgc aaaatttta caaataaaa ataacaaca 
      tacaagagc tagaaaagg atttatcaa aattgaacg 
      ttcaggatg gggtgcata aagggggta aacacgggg 
      gacagagac aaggcagcg tcccaccaa gactccaat 
      cggaacaaa agggctcca aacggtaca gttccatca 
      gacagaggt cgcttccag agtcggcaa cgccgccgt 
      tctcgcagc actcgaatt actactcaa atgaattca 
      accaagcac cgaatcaag tgaccattc atcaaaacg 
      cgcagattc atccgtcca cgactcaag tcccccaaa 
      tcgcgccgc cccctctgc cccccatcc cgcaggttc 
      ccgtcacgg cagccccca tcctgcagg cgccccaat 
      tcgcgcctc cgttcgccc aagctcccc cgtttttac 
      aaccggcgc cccctccat tcatccctc agcaaaccc 
      cccaaatta tcaactacg tgccgggtc aaccgcagt 
      tctctcagg ggtatcagg gctaccagc agcagttca 
      atcctgtga ctcagcctg agctgtacc agccccaga 
      cggggatca cgtactact cgactgacc aacaggtga 
      cgcagaggg cggcaccgc tcaagcgtc cgaaagctg 
      cgatcccga ttgtggcgc ctccgccga tcgatctga 
      aaggtaagg tcaaggagg aggacgcgt tggcaaccg 
      aggggtatg tgtcgactg ccaacgatg gacaaacaa 
      gcgacagtg gcagcgctc aggaatcaa atttacagg 
      attgtggtt atgccagaa gatatagtc tctattaat 
      ttacatatt ttagcttcc tttattgtt tatttgata 
      caaaagtta tggaaaatc aataaacgc cactaaaaa 
      agcgtcact tactaagtt tagctgata gatcattac 
      aagtgttct catttattt atgacagac attttactt 
      ttcattgtg taaagtttt tgtaatact acatgtatt 
      aaatattta aaaaatgt
    • >TC010061 RB
      ctagttggc accaacttg tttctctct gtttctttt 
      cctttcacg tcgttccat tgagcatga aaaggacca 
      gaaacgaga cgtaagtct tgaatttgt cactggcca 
      tcgaatctt gccaatccg gccaagata aagatattt 
      gtaaaacaa caaaaagat ggaagaaac cgttgctgt 
      gccagcgac agcccccca aacggtgga aaacatatc 
      acaggtgag cccaattgc tccttacgt tgctccttc 
      gttccggaa aagagaaag atttgagaa aaaattaac 
      aaaccaggt cacaagttc agaacgaag tggagcaca 
      accgaggcg atctcgcgt gaattcttc tcagtttaa 
      gaatgattt tgcaggaaa agacacgaa ttgacgctg 
      aggagagcc acaagtccc ctaacgagt cggagtatg 
      acagtgcag ggagttaca gtgatgaag aggaggata 
      aagcgaggt cgaggaatt ccagggagc acactgatt 
      cggaaactg aaggcagtg tagggctgg agcgggaat 
      ctggcgacg gacaggaga gtttgcctc tcaggaagg 
      tggacgatg atgaggaca agaaaaacc cccagtaca 
      tacccaaaa ggggcactt tttacgaac acgatgacc 
      ggacagctg atgacgagc aaacagctg ataatgtag 
      aattggaga aggaaaaag aacagaaga aaaaagtat 
      ggcaggata agaaggaaa ggtggagtc atgatcgtt 
      ttaacgact ctgagcaag cccccaagt ccagggctg 
      aacttgtgg ccatctatg ggtacgata ttaggaacg 
      aggaaggtc cccctcgag ctcgaaggc gcagacgat 
      acggacgtg gccctaaca agtatacaa gaaactggg 
      aagacgaag atgcctaca acaaaccgc cacctctta 
      ttcgtaaaa agaaacccc tcagaaagc ccaatcaag 
      gcgaagact ttccgcctc taaaccgct caagtaatc 
      aagaagacc cgcaggata gcagagatt acgaagaga 
      aaacagacc agtaccaag ctgaaacaa acggtaaca 
      cgaaaaatt cagtatgcg agtctccgc agtctccgc 
      cagtcccac cacaagaat ccaacagga tgcagcaac 
      gcgtcggtt ctggccgtg tggttaaac caaaacgcg 
      aaatcaagg attcagatt ataggggtt ttactacaa 
      aaacacgtc aagttagga atatgaaaa ctgatcaaa 
      aagtcgtcc cgaaatcct cgaaaaaag acgatttta 
      tccagtcgc aaaatttta caaataaaa ataacaaca 
      tacaagagc tagaaaagg atttatcaa aattgaacg 
      ttcaggatg gggtgcata aagggggta aacacgggg 
      gacagagac aaggcagcg tcccaccaa gactccaat 
      cggaacaaa agggctcca aacggtaca gttccatca 
      gacagaggt cgcttccag agtcggcaa cgccgccgt 
      tctcgcagc actcgaatt actactcaa atgaattca 
      accaagcac cgaatcaag tgaccattc atcaaaacg 
      cgcagattc atccgtcca cgactcaag tcccccaaa 
      tcgcgccgc cccctctgc cccccatcc cgcaggttc 
      ccgtcacgg cagccccca tcctgcagg cgccccaat 
      tcgcgcctc cgttcgccc aagctcccc cgtttttac 
      aaccggcgc cccctccat tcatccctc agcaaaccc 
      cccaaatta tcaactacg tgccgggtc aaccgcagt 
      tctctcagg ggtatcagg gctaccagc agcagttca 
      atcctgtga ctcagcctg agctgtacc agccccaga 
      cggggatca cgtactact cgactgacc aacaggtga 
      cgcagaggg cggcaccgc tcaagcgtc cgaaagctg 
      cgatcccga ttgtggcgc ctccgccga tcgatctga 
      aaggtaagg tcaaggagg aggacgcgt tggcaaccg 
      aggggtatg tgtcgactg ccaacgatg gacaaacaa 
      gcgacagtg gcagcgctc aggaatcaa atttacagg 
      tgcaacaat gatgtttgc agttttttg tgtaattct 
      tccgcgtac ggtatagct ggcttttta tatttaatg 
      aaatatcat atttatact tatattttg gaaaatcag 
      tttattaat acttaggaa caatggatt ttaaaatat 
      taattattt atgaaagta gcttagaca tgttttgtt 
      attgaaatg acgatattg aaggttatc atggttgaa 
      aattttatt gttgggtaa tttttactt tttttcagg 
      attgtggtt atgccagaa gatatagtc tctattaat 
      ttacatatt ttagcttcc tttattgtt tatttgata 
      caaaagtta tggaaaatc aataaacgc cactaaaaa 
      agcgtcact tactaagtt tagctgata gatcattac 
      aagtgttct catttattt atgacagac attttactt 
      ttcattgtg taaagtttt tgtaatact acatgtatt 
      aaatattta aaaaatgt
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