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Gene information
General information
TC012824 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15970551..15975191
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012824
    iBeetle screen iB_10509

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012824 RA
      MSSKGPPIT KLIVETSLE SADYDGVLL VSSPGQEIK 
      ATKLAGVLR DALLLDPTL ESEIAILPV PGLAAKRLV 
      HSPTGKIDP DYDDVRVLK NAALKGMKR ALKAGISKP 
      LLVLEETTR FENGDLVTL LGALEAVYV PIQVREHDP 
      TKPPIKHLG VFSTNPDKT KGLVHLATI LESGRRVAC 
      DIGGADPER MSPPNVEKY ILDVFSGSN IKVQVVKDP 
      EQFTREYPL FEAVNRAAS GVTRHQGRI VFLEYSPPD 
      PAQVKETVY LVGKGVTYD TGGIDVKIN GAMVGMSRD 
      KCGAAAVVG FLQVVRLLQ PKNVKVVAG VSLVRNSIG 
      SNGYVADEV ITARSGARV RVGNTDAEG RMVMADVLC 
      KMKELAVDA VNPHLFTVA TLTGHAFLT VGEGYSIVM 
      DNGPALKSG NSIRLQTAG DLIGDIFEI SRIRSEDFA 
      QHRGEAFGD DIKQANNQP SSRTPRGHQ GPAAFLILA 
      AGLETHGSG SGKPLKYSH LDIAASAGE FPKPATGAP 
      VLALAKAFL LN
    CDS sequence
    • >TC012824 RA
      atgtcctcc aaaggacct ccaatcacg aaactcatc 
      gtggaaact tcgctcgaa tcggccgat tacgacgga 
      gttcttttg gtttcatct cctggtcag gaaatcaaa 
      gcgacgaaa cttgctgga gtcctccgg gatgccctc 
      ctcctggat ccgaccctg gaatcggaa attgcaatt 
      ctgccggtt ccgggtcta gcggcgaag cgattggtg 
      cattcgccc accggaaaa atcgatccc gattacgat 
      gacgtcaga gtgttgaaa aatgcagct ttgaaaggc 
      atgaaaagg gcgctcaaa gcgggaatt tcgaaaccg 
      ttgcttgtt ttggaggag acgaccaga ttcgaaaac 
      ggggatttg gtgaccttg ttgggagcc ttggaagcg 
      gtatacgtg ccaattcaa gtgcgtgag cacgacccg 
      accaaacca ccaataaaa cacctgggg gtcttctcc 
      acaaacccg gacaaaacc aaaggtttg gtgcacctg 
      gccaccatc cttgaaagt ggccggcgg gttgcgtgc 
      gatattggg ggcgcagac ccggaacgg atgtcaccc 
      cccaacgtc gaaaagtac atcctcgac gtcttttcc 
      ggaagcaat attaaagtc caggtggtt aaagacccg 
      gaacagttc actcgggag tatccgctc tttgaggca 
      gtcaaccgg gccgcttcc ggagtcaca cgccaccaa 
      ggacgtatc gttttcctg gagtattcg cctccggat 
      ccggcccaa gtcaaggaa actgtctac cttgtcggg 
      aagggtgtc acttatgat acggggggt attgatgtt 
      aagattaac ggtgccatg gtggggatg tcaagggat 
      aaatgtggc gccgccgcc gtcgtggga ttcctccag 
      gtggtcaga ttactacaa ccgaaaaat gtcaaggtt 
      gtggctggg gtcagtcta gtccggaat agcataggg 
      tcaaatggt tacgtcgca gatgaggtc ataacggcc 
      aggtccgga gctcgagtc cgggtggga aatacggac 
      gcggaagga agaatggtc atggccgat gttttgtgc 
      aagatgaag gagttggct gtggatgcc gtcaatccg 
      catcttttc accgtcgcg acgctcaca ggacacgct 
      tttctaaca gtcggcgag ggatattcg attgtgatg 
      gacaacggt ccggctcta aaatccgga aattcgatc 
      cggttgcaa accgccggc gacttgatc ggcgacatt 
      ttcgaaatc tcccggatc cgatccgaa gactttgca 
      cagcatcgt ggcgaagct ttcggtgac gatatcaag 
      caggccaac aatcagcca tcatcaagg acaccgaga 
      ggacaccaa ggacctgcc gcattcctg atcctcgca 
      gctggattg gagacgcac ggatcagga agtgggaag 
      cccctgaaa tacagccat cttgatatc gccgcttcg 
      gcgggggaa ttccccaaa ccggccaca ggggctccg 
      gttttggca ctggctaag gcatttctg ttgaattaa
    mRNA sequence
    • >TC012824 RA
      ataaaaact caaacctcc cactcttca atttctcaa 
      aaaatcctc aataaaaac actcaacag cagtaaaac 
      cgtacgttt ctgagcgcc aacatgtcc tccaaagga 
      cctccaatc acgaaactc atcgtggaa acttcgctc 
      gaatcggcc gattacgac ggagttctt ttggtttca 
      tctcctggt caggaaatc aaagcgacg aaacttgct 
      ggagtcctc cgggatgcc ctcctcctg gatccgacc 
      ctggaatcg gaaattgca attctgccg gttccgggt 
      ctagcggcg aagcgattg gtgcattcg cccaccgga 
      aaaatcgat cccgattac gatgacgtc agagtgttg 
      aaaaatgca gctttgaaa ggcatgaaa agggcgctc 
      aaagcggga atttcgaaa ccgttgctt gttttggag 
      gagacgacc agattcgaa aacggggat ttggtgacc 
      ttgttggga gccttggaa gcggtatac gtgccaatt 
      caagtgcgt gagcacgac ccgaccaaa ccaccaata 
      aaacacctg ggggtcttc tccacaaac ccggacaaa 
      accaaaggt ttggtgcac ctggccacc atccttgaa 
      agtggccgg cgggttgcg tgcgatatt gggggcgca 
      gacccggaa cggatgtca ccccccaac gtcgaaaag 
      tacatcctc gacgtcttt tccggaagc aatattaaa 
      gtccaggtg gttaaagac ccggaacag ttcactcgg 
      gagtatccg ctctttgag gcagtcaac cgggccgct 
      tccggagtc acacgccac caaggacgt atcgttttc 
      ctggagtat tcgcctccg gatccggcc caagtcaag 
      gaaactgtc taccttgtc gggaagggt gtcacttat 
      gatacgggg ggtattgat gttaagatt aacggtgcc 
      atggtgggg atgtcaagg gataaatgt ggcgccgcc 
      gccgtcgtg ggattcctc caggtggtc agattacta 
      caaccgaaa aatgtcaag gttgtggct ggggtcagt 
      ctagtccgg aatagcata gggtcaaat ggttacgtc 
      gcagatgag gtcataacg gccaggtcc ggagctcga 
      gtccgggtg ggaaatacg gacgcggaa ggaagaatg 
      gtcatggcc gatgttttg tgcaagatg aaggagttg 
      gctgtggat gccgtcaat ccgcatctt ttcaccgtc 
      gcgacgctc acaggacac gcttttcta acagtcggc 
      gagggatat tcgattgtg atggacaac ggtccggct 
      ctaaaatcc ggaaattcg atccggttg caaaccgcc 
      ggcgacttg atcggcgac attttcgaa atctcccgg 
      atccgatcc gaagacttt gcacagcat cgtggcgaa 
      gctttcggt gacgatatc aagcaggcc aacaatcag 
      ccatcatca aggacaccg agaggacac caaggacct 
      gccgcattc ctgatcctc gcagctgga ttggagacg 
      cacggatca ggaagtggg aagcccctg aaatacagc 
      catcttgat atcgccgct tcggcgggg gaattcccc 
      aaaccggcc acaggggct ccggttttg gcactggct 
      aaggcattt ctgttgaat taattattc gtacttaat 
      aaagctcat tttcatgca aaagctcac gtacacttt 
      ggattcttg acttttggg tgcggtggc actgtgtgc 
      tatttttag gtcgaacta ttttaatta atattaaac 
      gtttttttt cttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY