Status
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Gene information
General information
TC011362 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 58306..67531
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011362
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_10505

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011362 RA
        MKYNTIQCG EKKSSFTIY ADLSSQDKM ASSEAEIAS 
        IRSELEIVQ KALKIQTKR CRQLVTEYT RKLQQKDLQ 
        YQNEKALRD NQLAKVLRA LIIFESRLK QEQKFISHQ 
        LKEKDYIIH KQSSEIKKL LSNQFCKNC NQFYNNTSS 
        SPNFESLES SSEYVDYQS SIDSSSDIY ATISEKSYE 
        PESKIFTKN VNNSRKMKK FQHRKSVGS YYEVLEAQN 
        DESSEEDKK LKISDSIPP FEGEETNNN WYASASDPD 
        DDEHRDIYR NNPVLECMN QILLQNIND SINSPPKTP 
        NTERKTLKK VKFEDEETK NNATLTKEN VEYYETPIQ 
        KVPNFYETP QSIYSNDYE QILSKCSES FVETKPKIR 
        KGTPPALPP KPANLTTLK YKVKKEPES VESEPDYCS 
        ISELNLPPK PVGRKINVV AEINPPLSQ DVIKKLDSG 
        GKSEPPPPE NLVKKCVEK INIQLAKQT PIKKPDPEI 
        PKLPQVSEI IIPEEEEKT DERISHDNY VKNNSQILK 
        GKKFEPNRR PIVMGTSVS SLISGFNNQ SLSEIRQKQ 
        RVEKAAFDL SQNFEEFKL DDCEIEEYS VSEEEVKSD 
        VDLDASSIR RSLEVQKTQ PTYEHFLEC TGLSSKSIL 
        TPSRLLSNH KSMLKPKDI KLRSKVKAN LFERHSTST 
        IKYWSEPYI
      CDS sequence
      • >TC011362 RA
        atgaagtac aatacgata caatgtggc gaaaagaag 
        tcctcgttt accatttat gcggattta tcgtcgcag 
        gataagatg gcgtccagc gaggccgaa atcgcctca 
        atccgctcc gagctcgaa atcgtccaa aaagccctc 
        aaaatccaa acgaaacgt tgccgccaa ttagtcacc 
        gaatacacg cgcaaactc caacaaaaa gacctccaa 
        taccaaaac gaaaaagca cttcgtgac aaccaactg 
        gctaaagtt ttgcgcgct ctcataatt ttcgaatca 
        cgcctcaaa caagaacaa aaattcatc agtcaccaa 
        cttaaagaa aaagactac attatccac aaacaaagc 
        agcgaaatc aaaaaactc ctctcgaac caattctgc 
        aaaaactgc aaccaattc tacaataac accagttca 
        agcccaaac tttgaaagc ttagagagc agttccgaa 
        tacgtcgac taccaaagc tcgattgac agcagcagc 
        gacatctat gcaacgatt tcggaaaaa agctacgag 
        cccgaaagt aaaattttc acgaaaaat gtcaacaac 
        tcccggaaa atgaaaaag tttcaacac cggaaaagt 
        gtagggagt tactatgaa gtgctggaa gcacaaaat 
        gatgaatcg agtgaagaa gacaagaaa ttgaagata 
        agtgactca atcccgcct tttgaaggt gaagaaact 
        aacaataac tggtacgca agtgcgagt gacccggac 
        gatgacgag cacagagac atctaccgg aataatccg 
        gtgctagag tgcatgaac caaatcctc ttgcaaaac 
        atcaatgac tcgatcaat tcaccgcca aaaacgcca 
        aacaccgaa cgcaaaact ttgaaaaaa gtcaaattc 
        gaagacgaa gaaacgaaa aataacgca acactgacg 
        aaagaaaat gtggaatat tacgaaaca ccgatccag 
        aaagtgcca aacttttac gaaacgcct cagagtatt 
        tacagtaat gactatgag cagattttg agtaaatgt 
        tcggagagt tttgttgaa actaagccg aaaattagg 
        aaagggact ccgccggct ttgccccca aagcctgcg 
        aatttaaca acgttgaag tataaagtg aagaaagag 
        ccagaaagt gttgagtct gaaccggat tattgctcg 
        atttctgag ctgaatctt ccgccaaag ccagtgggg 
        agaaagatt aatgttgtg gcggagatc aatccgcct 
        ttgtcgcag gatgttatc aaaaagtta gattctgga 
        gggaaaagt gaaccgcca ccacccgaa aacttggtg 
        aaaaaatgc gtggaaaag atcaacatc cagctcgcc 
        aaacaaacg ccgatcaaa aaaccggac ccggaaatt 
        cccaaactg cctcaagta tccgaaata ataatccca 
        gaagaagag gaaaaaaca gacgaaaga ataagtcac 
        gataactac gttaaaaac aattcccaa attctgaaa 
        ggcaaaaaa ttcgaaccc aacagacgt ccaattgtc 
        atggggacg tccgtttcg agcctaatt tccggtttc 
        aataaccaa agtttatcg gaaattcgg caaaaacag 
        cgcgttgaa aaagcagcg tttgacttg agccagaac 
        ttcgaggag ttcaaactg gacgattgt gagatagag 
        gagtacagt gtgagtgaa gaggaggtc aagtcggat 
        gtggatttg gacgcgagt tcaattagg aggtctctg 
        gaagtgcag aagacacaa ccgacctat gagcatttt 
        ttagagtgt actgggttg agttctaag tcgatttta 
        acgccttct aggctactt agtaaccat aaaagtatg 
        cttaagccc aaggacatt aaattgagg tccaaggtc 
        aaggcgaat ttgtttgag agacacagc acaagtact 
        attaaatac tggtcagag ccttacatt taa
      mRNA sequence
      • >TC011362 RA
        cgcttctta cctgtattt cctccgaag aggaacgtt 
        ttgcgcgcc accagcttg tggtcgcag gtgtggcgg 
        agcaaggga cgaatgtat aaccgttca caatcaact 
        caagtcgac gctgcttgg aattccatc caacgttta 
        tttcacaat tcgtcgcgg aatcgagca cacatcatg 
        atggtggaa gaaaaaagt gaagtgttg ttgaaaatt 
        ttttggtgg tgaagagcg caatcgaaa gagtgtgca 
        aaaatgaag tacaatacg atacaatgt ggcgaaaag 
        aagtcctcg tttaccatt tatgcggat ttatcgtcg 
        caggataag atggcgtcc agcgaggcc gaaatcgcc 
        tcaatccgc tccgagctc gaaatcgtc caaaaagcc 
        ctcaaaatc caaacgaaa cgttgccgc caattagtc 
        accgaatac acgcgcaaa ctccaacaa aaagacctc 
        caataccaa aacgaaaaa gcacttcgt gacaaccaa 
        ctggctaaa gttttgcgc gctctcata attttcgaa 
        tcacgcctc aaacaagaa caaaaattc atcagtcac 
        caacttaaa gaaaaagac tacattatc cacaaacaa 
        agcagcgaa atcaaaaaa ctcctctcg aaccaattc 
        tgcaaaaac tgcaaccaa ttctacaat aacaccagt 
        tcaagccca aactttgaa agcttagag agcagttcc 
        gaatacgtc gactaccaa agctcgatt gacagcagc 
        agcgacatc tatgcaacg atttcggaa aaaagctac 
        gagcccgaa agtaaaatt ttcacgaaa aatgtcaac 
        aactcccgg aaaatgaaa aagtttcaa caccggaaa 
        agtgtaggg agttactat gaagtgctg gaagcacaa 
        aatgatgaa tcgagtgaa gaagacaag aaattgaag 
        ataagtgac tcaatcccg ccttttgaa ggtgaagaa 
        actaacaat aactggtac gcaagtgcg agtgacccg 
        gacgatgac gagcacaga gacatctac cggaataat 
        ccggtgcta gagtgcatg aaccaaatc ctcttgcaa 
        aacatcaat gactcgatc aattcaccg ccaaaaacg 
        ccaaacacc gaacgcaaa actttgaaa aaagtcaaa 
        ttcgaagac gaagaaacg aaaaataac gcaacactg 
        acgaaagaa aatgtggaa tattacgaa acaccgatc 
        cagaaagtg ccaaacttt tacgaaacg cctcagagt 
        atttacagt aatgactat gagcagatt ttgagtaaa 
        tgttcggag agttttgtt gaaactaag ccgaaaatt 
        aggaaaggg actccgccg gctttgccc ccaaagcct 
        gcgaattta acaacgttg aagtataaa gtgaagaaa 
        gagccagaa agtgttgag tctgaaccg gattattgc 
        tcgatttct gagctgaat cttccgcca aagccagtg 
        gggagaaag attaatgtt gtggcggag atcaatccg 
        cctttgtcg caggatgtt atcaaaaag ttagattct 
        ggagggaaa agtgaaccg ccaccaccc gaaaacttg 
        gtgaaaaaa tgcgtggaa aagatcaac atccagctc 
        gccaaacaa acgccgatc aaaaaaccg gacccggaa 
        attcccaaa ctgcctcaa gtatccgaa ataataatc 
        ccagaagaa gaggaaaaa acagacgaa agaataagt 
        cacgataac tacgttaaa aacaattcc caaattctg 
        aaaggcaaa aaattcgaa cccaacaga cgtccaatt 
        gtcatgggg acgtccgtt tcgagccta atttccggt 
        ttcaataac caaagttta tcggaaatt cggcaaaaa 
        cagcgcgtt gaaaaagca gcgtttgac ttgagccag 
        aacttcgag gagttcaaa ctggacgat tgtgagata 
        gaggagtac agtgtgagt gaagaggag gtcaagtcg 
        gatgtggat ttggacgcg agttcaatt aggaggtct 
        ctggaagtg cagaagaca caaccgacc tatgagcat 
        tttttagag tgtactggg ttgagttct aagtcgatt 
        ttaacgcct tctaggcta cttagtaac cataaaagt 
        atgcttaag cccaaggac attaaattg aggtccaag 
        gtcaaggcg aatttgttt gagagacac agcacaagt 
        actattaaa tactggtca gagccttac atttaactg 
        tattaacgt tttaccaat atttatcag cttatctat 
        tgtacatac ggtattgtt atcaataaa tttatttat 
        ttcaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY