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Gene information
General information
TC008948 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12489053..12491031
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008948
    iBeetle screen iB_10504

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008948 RA
      MEGRVILGQ IIHCINLNT LSVLDNGFV LIGIDGKIK 
      ATGNASDLE SAKKQLNFT RLETITLKK KQILLPGFI 
      DTHIHAPQY PNAGLGYDK PLLEWLDSY TYKLEKKYK 
      DLELSKKVY DAVVRKTLD YGTTTACYF ASLYDDSSL 
      ILANSVTKF GQRAFVGKV NMTKLAPSD YVETAQESI 
      DNTLKFIRN VRAINNPLV QPIITPRFA LSVDMDVMK 
      KLSLIAKEY NLNIQTHIS ENKDEVKMV HETYNDLYA 
      SVYHTANLL TPRTVLAHG IHLSEDEMK LLHKTGTSI 
      SHCPESNVY LSSGICDVR KLWEHGINV ALGTDVSGG 
      ASPSIINAM RSAISASTN LSFTKSKYT KLTYVDVFY 
      MATLGGAKA LALDNEIGN FEVGKSFDA VIVDLDIEH 
      SSADLLSEC TPQELLQKL VFLGDDRNV VKVFVNGKI 
      VKD
    CDS sequence
    • >TC008948 RA
      atggaaggc agagtcatt ttgggccaa attatacat 
      tgcataaat ctcaacact ttatcagtg ctcgataat 
      ggttttgtt ctcatagga attgatggc aagattaaa 
      gcaactgga aacgcctcc gacttagag tcagccaaa 
      aaacaactc aattttaca agacttgaa acaattaca 
      ttgaaaaaa aagcaaata cttcttccc ggtttcatt 
      gatactcat atacacgca ccacaatat cctaatgca 
      ggattaggg tacgataaa ccccttctt gaatggctt 
      gattcctac acttacaaa ttagaaaaa aagtacaaa 
      gatttggaa ctcagcaaa aaagtttat gacgctgtt 
      gtgcgaaag actttagac tacggtacc accactgcg 
      tgttatttc gcatcatta tatgatgat agttcttta 
      attttggct aattctgtg accaaattt ggacagaga 
      gcctttgtg ggaaaagtc aatatgacc aaacttgca 
      ccttcggat tatgtggaa acggcacaa gaatcgatt 
      gataatact ttgaaattt ataagaaat gttcgtgct 
      attaataat cctttggtc caaccgatt attactcca 
      aggtttgcc ttaagtgtc gatatggat gtcatgaaa 
      aaattaagt ctaattgcg aaagaatac aatcttaac 
      atccaaacc cacatttcg gaaaataaa gacgaggta 
      aaaatggta catgaaacg tataatgat ttgtacgct 
      agtgtttac cacactgct aatctttta acaccgagg 
      acagtttta gcccacggt attcacttg agcgaggac 
      gaaatgaag ctacttcat aaaacgggg acttcaatt 
      tcgcattgt cctgaatca aatgtttac ttgagttcg 
      ggaatttgt gatgtgcga aaattatgg gaacatggg 
      attaacgtg gctttgggg actgacgtc tctggagga 
      gcatcgcct tcaattata aatgcgatg aggtctgct 
      attagtgcg tctacaaat ctttcgttt acgaaatca 
      aaatacaca aaactcact tacgttgat gttttttat 
      atggcaaca ttaggcgga gctaaagct ttggcttta 
      gataacgaa atcggtaat tttgaagtt ggaaaaagt 
      ttcgacgct gttatcgtc gatttagat atcgaacat 
      tcttcagcg gacttatta tcagagtgt acaccacaa 
      gaattacta caaaaattg gtgttttta ggcgacgac 
      aggaatgta gtaaaagtt tttgttaat gggaaaatt 
      gttaaagat tag
    mRNA sequence
    • >TC008948 RA
      tttgaagac cgcataacg acagtaatc atttcactt 
      acacacaag ataaaatat gtaaactac caaaaacaa 
      tacacaaac taaacatct gattctact gcttatctc 
      ccccctcaa acttatagt tgtttatcg cctgtagaa 
      ctttatccg ttttgtagt ttgtttttc tgtatattc 
      ataacaatg gaaggcaga gtcattttg ggccaaatt 
      atacattgc ataaatctc aacacttta tcagtgctc 
      gataatggt tttgttctc ataggaatt gatggcaag 
      attaaagca actggaaac gcctccgac ttagagtca 
      gccaaaaaa caactcaat tttacaaga cttgaaaca 
      attacattg aaaaaaaag caaatactt cttcccggt 
      ttcattgat actcatata cacgcacca caatatcct 
      aatgcagga ttagggtac gataaaccc cttcttgaa 
      tggcttgat tcctacact tacaaatta gaaaaaaag 
      tacaaagat ttggaactc agcaaaaaa gtttatgac 
      gctgttgtg cgaaagact ttagactac ggtaccacc 
      actgcgtgt tatttcgca tcattatat gatgatagt 
      tctttaatt ttggctaat tctgtgacc aaatttgga 
      cagagagcc tttgtggga aaagtcaat atgaccaaa 
      cttgcacct tcggattat gtggaaacg gcacaagaa 
      tcgattgat aatactttg aaatttata agaaatgtt 
      cgtgctatt aataatcct ttggtccaa ccgattatt 
      actccaagg tttgcctta agtgtcgat atggatgtc 
      atgaaaaaa ttaagtcta attgcgaaa gaatacaat 
      cttaacatc caaacccac atttcggaa aataaagac 
      gaggtaaaa atggtacat gaaacgtat aatgatttg 
      tacgctagt gtttaccac actgctaat cttttaaca 
      ccgaggaca gttttagcc cacggtatt cacttgagc 
      gaggacgaa atgaagcta cttcataaa acggggact 
      tcaatttcg cattgtcct gaatcaaat gtttacttg 
      agttcggga atttgtgat gtgcgaaaa ttatgggaa 
      catgggatt aacgtggct ttggggact gacgtctct 
      ggaggagca tcgccttca attataaat gcgatgagg 
      tctgctatt agtgcgtct acaaatctt tcgtttacg 
      aaatcaaaa tacacaaaa ctcacttac gttgatgtt 
      ttttatatg gcaacatta ggcggagct aaagctttg 
      gctttagat aacgaaatc ggtaatttt gaagttgga 
      aaaagtttc gacgctgtt atcgtcgat ttagatatc 
      gaacattct tcagcggac ttattatca gagtgtaca 
      ccacaagaa ttactacaa aaattggtg tttttaggc 
      gacgacagg aatgtagta aaagttttt gttaatggg 
      aaaattgtt aaagattag tgtctaata aaccctata 
      agagact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY