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Gene information
General information
TC009509 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1684113..1686478
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC009509
      iBeetle screen iB_10503

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009509 RA
        MPRSGLVTF LISVAFLIR CFQGQKTET STSAESLMI 
        DTTTESTKE EGAYIDMEI ISIEVLPKI GNAVVLSWG 
        KPLREYDLD WTYGVYYGE NMQEALKEP KLTTKDLSA 
        EVANLTFCT QYIFAVGVV DPASKRPHP ARNVRTIGT 
        LINELSPPE DLQVEFIPE KEPCLLIKW SASCPNIAE 
        PLGYLVTVH EKNSPRMML ITHPFTTSV ELAQTLRVV 
        YGKSYDIKV ATDVVGSQP TETVSFKVP HFLQPYKVR 
        LTSRPEGAF VMYWREPFV PYFVDDFFY EVYVYPGRD 
        LQGKAQIYA TERPVFQFQ GNQSEYTFM VGLRSGDGE 
        FKSYLTDPV AINLLGENV TLSVS
      CDS sequence
      • >TC009509 RA
        atgccccgt tctggttta gtaactttt ttaatttcg 
        gttgcgttt ctaattcgc tgtttccag ggccagaaa 
        actgaaact tcgacttct gctgaatcc ttaatgatt 
        gacacgacg acggaaagc accaaagaa gagggtgct 
        tatattgac atggaaatt atcagcatt gaagtgcta 
        ccgaaaatt ggcaatgct gtggtcctg tcctggggg 
        aaaccccta cgagagtat gacctggat tggacttac 
        ggcgtctac tacggcgaa aatatgcag gaagctctg 
        aaagagcca aaattgacc accaaagac ctttccgcc 
        gaagtggca aacctgacc ttttgcacg cagtacatc 
        ttcgccgtg ggggtggtg gaccccgcc tccaaacgc 
        ccacacccg gcccgcaac gtgcgaaca atcggcact 
        ctcatcaac gagctttct ccaccagag gacctccag 
        gtggaattt atccccgag aaggaacct tgtctttta 
        atcaaatgg tcggcttcg tgccccaac atcgccgag 
        cctctcgga tacctggtg accgtccac gagaaaaac 
        tccccgagg atgatgctc ataacacac cctttcacc 
        acctcggtg gagttggcc caaactttg cgcgtggtt 
        tacggcaag tcgtacgac attaaagtc gccactgac 
        gtggtgggg tcgcaaccc acggagacg gtgtcgttc 
        aaagtgccc cattttttg caaccgtac aaagtgcga 
        ttgacttca cgtcccgaa ggcgcgttc gtgatgtac 
        tggcgcgag ccgttcgtg ccctacttc gtggacgac 
        ttcttctac gaggtgtac gtgtacccg gggcgggat 
        ctgcaaggc aaggcgcag atctacgcc acggagcgg 
        cccgtgttc cagtttcag gggaatcag agcgagtac 
        accttcatg gtggggctt aggtcagga gatggggag 
        ttcaagtcg tatctgacg gacccggtg gcgataaac 
        ttgctcggg gagaatgtg acgctcagt gttagctga
      mRNA sequence
      • >TC009509 RA
        tttcatcaa taatgtggt gcccacaca gtgtgacaa 
        aatgtagtc atttagaac attagcaaa caatgtgct 
        aaatgaaaa ataattgcg gccaaattt ggttcccgc 
        tttatttta ttccgcaat tcaagctgt ttggaaaac 
        ccccccttg aacgatttt tataagtcc atcagctgc 
        tgtattatt tatttttat ttgacattg ggaatcgtc 
        gtcatccgt tatgtgtgt ttcgttcct aacagtgta 
        tttgcagtt tgacagcat gccccgttc tggtttagt 
        aactttttt aatttcggt tgcgtttct aattcgctg 
        tttccaggg ccagaaaac tgaaacttc gacttctgc 
        tgaatcctt aatgattga cacgacgac ggaaagcac 
        caaagaaga gggtgctta tattgacat ggaaattat 
        cagcattga agtgctacc gaaaattgg caatgctgt 
        ggtcctgtc ctgggggaa acccctacg agagtatga 
        cctggattg gacttacgg cgtctacta cggcgaaaa 
        tatgcagga agctctgaa agagccaaa attgaccac 
        caaagacct ttccgccga agtggcaaa cctgacctt 
        ttgcacgca gtacatctt cgccgtggg ggtggtgga 
        ccccgcctc caaacgccc acacccggc ccgcaacgt 
        gcgaacaat cggcactct catcaacga gctttctcc 
        accagagga cctccaggt ggaatttat ccccgagaa 
        ggaaccttg tcttttaat caaatggtc ggcttcgtg 
        ccccaacat cgccgagcc tctcggata cctggtgac 
        cgtccacga gaaaaactc cccgaggat gatgctcat 
        aacacaccc tttcaccac ctcggtgga gttggccca 
        aactttgcg cgtggttta cggcaagtc gtacgacat 
        taaagtcgc cactgacgt ggtggggtc gcaacccac 
        ggagacggt gtcgttcaa agtgcccca ttttttgca 
        accgtacaa agtgcgatt gacttcacg tcccgaagg 
        cgcgttcgt gatgtactg gcgcgagcc gttcgtgcc 
        ctacttcgt ggacgactt cttctacga ggtgtacgt 
        gtacccggg gcgggatct gcaaggcaa ggcgcagat 
        ctacgccac ggagcggcc cgtgttcca gtttcaggg 
        gaatcagag cgagtacac cttcatggt ggggcttag 
        gtcaggaga tggggagtt caagtcgta tctgacgga 
        cccggtggc gataaactt gctcgggga gaatgtgac 
        gctcagtgt tagctgaag cgagtgtcg gtttcaaag 
        tgttgtttg agtgaactt tcaaattgg attcggggt 
        ccatttttt gcagttttg tcatttcat cgtttattg 
        taatggaaa taaaacttt aaacttttg gggaaaact 
        ttgatactc aaataaagg tagttgcaa gttactgac 
        tctatttgg acgtcagtt cttgtttgc gggaggctg 
        caggcgaaa ctgactgtg gttatagcc agagttgca 
        tgtttccaa tgaagtcgt acctcagga gctttgcag 
        acgggaagt gtttttaaa ctgaaaact atcagcttt 
        ggagtgcaa atagcggaa aaacgtata agaaaaatt 
        tttctagat tttttaagc gctctcaaa gaaaaacat 
        tttttttgt gtgccgagt aagaaaaaa aatttttat 
        at
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY