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Gene information
General information
TC004496 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3752373..3758780
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004496
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03770

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: mostly legs
    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_10501

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 1

    vitellogenic egg chamber degenerated - Number of animals showing the phenotype: 2
    vitellarium size decreased - Number of animals showing the phenotype: 2

    mature egg not fused - Number of animals showing the phenotype: 3
    lateral oviduct degenerated - Number of animals showing the phenotype: 3
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004496 RA
      MTSIEEKIR KLGQNRDIE GLQDYVSNL SLEELVNVI 
RTRLTNSNF TYTWNYILE GLAESPNSH NKRYGVVLS 
VIEELDRSE VSSFHSNAI TSRICTELA KFQTEHLLK 
LCDFCVESL QSGRVSQMS WKEILPQLL NVLSKKEHV 
DVEGEEISG IEYKHQVIN NICTLQWSP NIVVNLTQM 
FIDLSLSKE EHLQVVNKL AKQLEKMTP QEVPPFVYQ 
LLKFCKNSS ASVIFIKLQ HYFALRYHD KFLDQTASS 
SESSEMDLI EDASSQDIL EAEATVLYH IHSLAFVGH 
NCIKDHLNF LKNMLRTPE FILHPFQLS VLLTLSTIP 
RYEERVFEI IRSCVSRTF HEEQKKISS ASFRNLVPG 
KTRLEIIFG HVVTASAQD RDLVLQGLI NFAFVLLGV 
GSALGRDPI AEKQWQLGS IILIKIIKR KRHTASIIV 
ETLCNKIVT EQSVTQYIE CLYLMTRKL ALLMLENKN 
TIISLMGGL IEIPLTSAL LVLDAIIPL TKVSPTIRD 
HFILLLHKA LHSRVTETR QVAVTGYLK LLANLKISD 
LAILSQGSY SSGYSLYTQ ISITQTSQC TPSAFSNEA 
LSLEVLNNL EACFMQPAE VRIQLYTGL YDAVCLNPD 
LGIAVLETI WPHFCDFYN TDEETAVPL KFDKITLTR 
DINVSLQEP LGKLIYTIG LIVTKVAKD NNETPTVAR 
ILKVLESIC QRMIKCELV HFELDDGTD LGDVMPESQ 
HKLFVLKEA MSVYEALIG YKFHSWELD SENNGKQIK 
GLFEGYLRL SNCLKTVNK PKKSDKNKT ENDTTLKKE 
QKSSNRLKL PETVLDFQV LSKIMSFLH ETMVSWTTC 
VQANVVKSK REFHQHAMQ ATLNLVTSL KHKKNIETQ 
TAKVLYDHL CDIAMVIYE RIIKRMDEF ASFDCTTAV 
LAAECFLLI LNIVNNHHG SNLKAFLSR VGGRDTSED 
LVSHLSEFI QTYQKLFET DEDEISEDP EVKKMSLIV 
INTLSFFAH QIPPNSNTL ALQMMEWTK NYALNNDLK 
GRQVVNGFI TLLFECHVN FKVSLTFFE QVSDHIGDV 
TGVITEEEH TSEEMKVLN EQTVNTVFT CLCNNIKSV 
LEDIDWVIA RLKAEFSLM TYPNDEGLE RRRENLQSR 
ERGVCCQLC FIVTILTNL SNCTIPPGG LSEALLKNL 
FLLYCTLSS LTKHFVLRS SKINPVFQN AKFERLVKL 
AGKQLAPAV YKLITYIEE TQKEEPESP SKKSKKKVE 
STTLKSKVL KETRLIPKV VYEIEQFGK LVAQLSNKT 
KIDLAKFMG QGTVRGFRF LNWEQVVEK ANDGAPQTQ 
DSEENNEES DEETSPPPS KQRKT
    CDS sequence
    • >TC004496 RA
      atgacttcc attgaggag aaaatacgt aaattgggc 
caaaatcgc gacattgag ggcctgcaa gactacgtt 
agcaattta agtttggag gaacttgta aatgtgatt 
cggacacga ttaactaac tcgaatttt acgtatact 
tggaattac attctggaa ggtttggcg gaaagtccc 
aactctcat aacaagcgc tatggggtt gttttgagt 
gtcattgaa gaattggac aggagtgag gtttcaagc 
tttcacagt aatgctatt acgagccga atttgtacg 
gaacttgct aagttccaa acggaacat ttgttaaaa 
ttgtgtgat ttttgtgtt gagtcttta cagtcgggc 
agagtttca caaatgagt tggaaagag atattgccc 
caattattg aacgtttta agtaagaaa gaacatgtt 
gatgtggag ggtgaagaa atatcaggc attgaatat 
aagcatcaa gtcattaac aacatttgc actttgcag 
tggtctcct aatatcgtt gttaattta actcagatg 
tttatcgac ctttctctt tctaaagaa gaacacttg 
caagtcgtt aacaagttg gcaaaacag ttggaaaaa 
atgacgccg caagaggtc ccacctttt gtttaccag 
ttgcttaaa ttttgtaaa aatagcagt gctagcgtc 
attttcata aaattgcag cactatttc gccctgcga 
tatcatgat aaattcttg gaccaaacc gcctcaagt 
tcagagagt tcagaaatg gatttaatc gaggatgct 
tcaagtcaa gacattcta gaagcagaa gcaacagtt 
ctgtaccac atccacagt ttagctttc gtaggccac 
aactgcatt aaagaccat ttaaatttc ctaaaaaac 
atgttgagg acacccgaa tttattctc caccccttc 
caattgagt gtcctactg accctatcc acaatacca 
cgctacgag gaacgagtt tttgaaatc atcaggtcg 
tgtgtctct cgaacattt cacgaagaa cagaagaaa 
atttcctcg gcttcgttc aggaacttg gtgcctggc 
aaaaccaga cttgaaata attttcgga catgttgtg 
accgcaagt gcccaagac cgtgacttg gttcttcag 
ggacttatc aattttgcc tttgtgcta cttggagtg 
ggctctgct ttgggtcga gacccaatc gctgaaaaa 
cagtggcaa ttagggagt attatctta attaagata 
attaaacgg aaacggcac actgcgtcc atcatagtg 
gaaactctg tgtaataaa atagtgacc gaacagtca 
gtgactcaa tatattgaa tgtctttat ttgatgact 
cgaaaattg gcgcttcta atgctagaa aacaaaaat 
acgattatt tcgctaatg ggaggcttg attgagata 
ccattaacc agtgcttta ctagtacta gacgcgatt 
attccactc actaaagtc tcacctacc atccgagac 
catttcatt ctcttgttg cacaaagca ctccattca 
agagtgacc gaaacgcgc caagttgca gtcactggt 
tacttaaaa ttattagca aatttaaaa atctcagat 
ttagctatt ttgagtcaa ggttcttac tcatcaggt 
tattcgtta tatactcaa atttccatt actcaaaca 
tcacaatgc acaccaagt gccttcagt aacgaagca 
ctctcttta gaagttttg aacaacctg gaggcgtgc 
ttcatgcaa ccagccgaa gtcagaatt cagctatac 
acaggtctc tacgacgct gtttgtttg aatcccgat 
ttggggatc gcagttttg gaaacgatt tggccacat 
ttttgcgat ttttacaat actgacgag gaaacggcc 
gttccgttg aagtttgat aaaattaca ctcactcga 
gacatcaat gtttcgtta caagagcct ttgggtaaa 
ttaatttac acaatcggg cttattgtc acaaaagtg 
gcgaaagat aacaacgaa actcccaca gttgcccga 
attctaaag gtcctagaa tcaatttgt caaagaatg 
attaagtgt gaattggtt cattttgaa ttggatgac 
ggtaccgat ttgggcgat gtaatgccc gaatctcag 
cacaaactt ttcgttctg aaggaagca atgagtgtt 
tatgaggct ttgattggg tacaagttt cactcgtgg 
gaactagac tcggaaaat aacggcaaa caaatcaag 
ggtttgttt gaaggttac ttgcgactg tccaattgt 
ttaaaaacc gttaacaaa ccaaaaaaa tcggacaag 
aacaaaaca gaaaacgac acaacgttg aaaaaagaa 
caaaagtca tcaaacaga ttaaaatta ccggagacg 
gtgctcgat ttccaagtt ttgtcaaaa attatgagt 
tttctacac gagacaatg gtttcgtgg accacatgc 
gtccaagcc aatgttgtt aaatcaaag agagaattt 
caccaacac gccatgcaa gcaactctc aatttagtt 
acctcacta aaacacaaa aaaaatatt gaaacgcag 
actgctaaa gtactttat gaccacttg tgcgatatt 
gcgatggtc atttatgaa agaataatc aaacgaatg 
gacgaattt gcaagtttt gattgcaca acagctgtt 
ttagcagct gagtgtttt ctgttaatt ttgaacatt 
gttaacaac caccatggg agtaatttg aaagcattt 
ttgagtcga gttggtggt agagacaca agcgaagac 
cttgtcagc catttgagc gagtttatc caaacttat 
cagaaatta tttgaaacg gatgaagac gaaatttcc 
gaggatcca gaagttaaa aaaatgtcg ctcattgtg 
atcaatact ctgagcttc ttcgcacat caaattccc 
ccaaatagc aatacctta gcgttacaa atgatggaa 
tggacgaaa aattacgct cttaataac gatttaaaa 
ggcagacaa gtcgtcaac gggtttatc acgttattg 
tttgaatgc cacgttaac ttcaaagtt agtttaact 
ttttttgaa caggtgtct gaccacatt ggtgatgtc 
acaggtgtg atcactgag gaggaacac accagtgaa 
gaaatgaaa gttttgaat gaacaaacc gtaaatacc 
gtttttacg tgtttgtgt aacaatatc aaatcagtt 
cttgaagat atcgattgg gttatcgca cgtttgaaa 
gctgaattt tctctaatg acttatccc aatgatgaa 
ggtttggag agaagacgt gaaaactta caatcacgc 
gaacgtgga gtttgttgc cagttatgt ttcatagtc 
acaatttta acaaatttg agcaattgt actatccct 
cccgggggg ctgtctgag gccctttta aaaaatttg 
tttttgttg tattgcacg ttgagttcg ctaacaaaa 
catttcgtt ttgcggtcg tccaaaatc aacccggtc 
ttccaaaat gccaaattt gagcgatta gttaaatta 
gctggcaaa caattggct ccagctgtt tacaagctg 
attacttat atcgaggag actcagaag gaagagcca 
gaatcgccg tcgaaaaag tcaaagaaa aaagttgaa 
tcgaccact ttgaaaagc aaagtgctg aaggagaca 
cgacttata ccgaaagta gtttatgaa attgaacag 
tttggtaaa ttagttgcg cagttgtct aacaagact 
aaaattgat ttggccaag ttcatgggt caggggaca 
gttcgaggt tttaggttt ttgaattgg gaacaagtt 
gtggagaag gctaatgat ggggcacca caaacacaa 
gatagtgaa gaaaataat gaagagagt gacgaggaa 
acgtcacca cctccgtca aaacaacgc aaaacttaa
    mRNA sequence
    • >TC004496 RA
      agtcaactg aaataaaca attccacat tcaaattct 
acaatttct gtgcatttt ccaataatt cggaatgaa 
ttcgccaat ttcgcgaat tattagacg aagcccctt 
gcttctgag acaaggaag tgtttcaaa aaatttcgt 
acgcacgga gctatgcgg tggagtcct tggagagaa 
tatgccgtt caagtttca caacaggtt cactttcac 
cctaaaatg ttggctatt ttttgtgcc cgcattttg 
ggcccctca aatcgtcgt ggcttacca acttctcaa 
atagttatc aatacaaat tgtagtttt tctaataaa 
tgattgctt tttaaattt agcgcgaaa tttcaatag 
tttacaaac aactgccca cgacataac ctcatattt 
tcaaaaagt ttcgctcca aaatgactt ccattgagg 
agaaaatac gtaaattgg gccaaaatc gcgacattg 
agggcctgc aagactacg ttagcaatt taagtttgg 
aggaacttg taaatgtga ttcggacac gattaacta 
actcgaatt ttacgtata cttggaatt acattctgg 
aaggtttgg cggaaagtc ccaactctc ataacaagc 
gctatgggg ttgttttga gtgtcattg aagaattgg 
acaggagtg aggtttcaa gctttcaca gtaatgcta 
ttacgagcc gaatttgta cggaacttg ctaagttcc 
aaacggaac atttgttaa aattgtgtg atttttgtg 
ttgagtctt tacagtcgg gcagagttt cacaaatga 
gttggaaag agatattgc cccaattat tgaacgttt 
taagtaaga aagaacatg ttgatgtgg agggtgaag 
aaatatcag gcattgaat ataagcatc aagtcatta 
acaacattt gcactttgc agtggtctc ctaatatcg 
ttgttaatt taactcaga tgtttatcg acctttctc 
tttctaaag aagaacact tgcaagtcg ttaacaagt 
tggcaaaac agttggaaa aaatgacgc cgcaagagg 
tcccacctt ttgtttacc agttgctta aattttgta 
aaaatagca gtgctagcg tcattttca taaaattgc 
agcactatt tcgccctgc gatatcatg ataaattct 
tggaccaaa ccgcctcaa gttcagaga gttcagaaa 
tggatttaa tcgaggatg cttcaagtc aagacattc 
tagaagcag aagcaacag ttctgtacc acatccaca 
gtttagctt tcgtaggcc acaactgca ttaaagacc 
atttaaatt tcctaaaaa acatgttga ggacacccg 
aatttattc tccacccct tccaattga gtgtcctac 
tgaccctat ccacaatac cacgctacg aggaacgag 
tttttgaaa tcatcaggt cgtgtgtct ctcgaacat 
ttcacgaag aacagaaga aaatttcct cggcttcgt 
tcaggaact tggtgcctg gcaaaacca gacttgaaa 
taattttcg gacatgttg tgaccgcaa gtgcccaag 
accgtgact tggttcttc agggactta tcaattttg 
cctttgtgc tacttggag tgggctctg ctttgggtc 
gagacccaa tcgctgaaa aacagtggc aattaggga 
gtattatct taattaaga taattaaac ggaaacggc 
acactgcgt ccatcatag tggaaactc tgtgtaata 
aaatagtga ccgaacagt cagtgactc aatatattg 
aatgtcttt atttgatga ctcgaaaat tggcgcttc 
taatgctag aaaacaaaa atacgatta tttcgctaa 
tgggaggct tgattgaga taccattaa ccagtgctt 
tactagtac tagacgcga ttattccac tcactaaag 
tctcaccta ccatccgag accatttca ttctcttgt 
tgcacaaag cactccatt caagagtga ccgaaacgc 
gccaagttg cagtcactg gttacttaa aattattag 
caaatttaa aaatctcag atttagcta ttttgagtc 
aaggttctt actcatcag gttattcgt tatatactc 
aaatttcca ttactcaaa catcacaat gcacaccaa 
gtgccttca gtaacgaag cactctctt tagaagttt 
tgaacaacc tggaggcgt gcttcatgc aaccagccg 
aagtcagaa ttcagctat acacaggtc tctacgacg 
ctgtttgtt tgaatcccg atttgggga tcgcagttt 
tggaaacga tttggccac atttttgcg atttttaca 
atactgacg aggaaacgg ccgttccgt tgaagtttg 
ataaaatta cactcactc gagacatca atgtttcgt 
tacaagagc ctttgggta aattaattt acacaatcg 
ggcttattg tcacaaaag tggcgaaag ataacaacg 
aaactccca cagttgccc gaattctaa aggtcctag 
aatcaattt gtcaaagaa tgattaagt gtgaattgg 
ttcattttg aattggatg acggtaccg atttgggcg 
atgtaatgc ccgaatctc agcacaaac ttttcgttc 
tgaaggaag caatgagtg tttatgagg ctttgattg 
ggtacaagt ttcactcgt gggaactag actcggaaa 
ataacggca aacaaatca agggtttgt ttgaaggtt 
acttgcgac tgtccaatt gtttaaaaa ccgttaaca 
aaccaaaaa aatcggaca agaacaaaa cagaaaacg 
acacaacgt tgaaaaaag aacaaaagt catcaaaca 
gattaaaat taccggaga cggtgctcg atttccaag 
ttttgtcaa aaattatga gttttctac acgagacaa 
tggtttcgt ggaccacat gcgtccaag ccaatgttg 
ttaaatcaa agagagaat ttcaccaac acgccatgc 
aagcaactc tcaatttag ttacctcac taaaacaca 
aaaaaaata ttgaaacgc agactgcta aagtacttt 
atgaccact tgtgcgata ttgcgatgg tcatttatg 
aaagaataa tcaaacgaa tggacgaat ttgcaagtt 
ttgattgca caacagctg ttttagcag ctgagtgtt 
ttctgttaa ttttgaaca ttgttaaca accaccatg 
ggagtaatt tgaaagcat ttttgagtc gagttggtg 
gtagagaca caagcgaag accttgtca gccatttga 
gcgagttta tccaaactt atcagaaat tatttgaaa 
cggatgaag acgaaattt ccgaggatc cagaagtta 
aaaaaatgt cgctcattg tgatcaata ctctgagct 
tcttcgcac atcaaattc ccccaaata gcaatacct 
tagcgttac aaatgatgg aatggacga aaaattacg 
ctcttaata acgatttaa aaggcagac aagtcgtca 
acgggttta tcacgttat tgtttgaat gccacgtta 
acttcaaag ttagtttaa ctttttttg aacaggtgt 
ctgaccaca ttggtgatg tcacaggtg tgatcactg 
aggaggaac acaccagtg aagaaatga aagttttga 
atgaacaaa ccgtaaata ccgttttta cgtgtttgt 
gtaacaata tcaaatcag ttcttgaag atatcgatt 
gggttatcg cacgtttga aagctgaat tttctctaa 
tgacttatc ccaatgatg aaggtttgg agagaagac 
gtgaaaact tacaatcac gcgaacgtg gagtttgtt 
gccagttat gtttcatag tcacaattt taacaaatt 
tgagcaatt gtactatcc ctcccgggg ggctgtctg 
aggcccttt taaaaaatt tgtttttgt tgtattgca 
cgttgagtt cgctaacaa aacatttcg ttttgcggt 
cgtccaaaa tcaacccgg tcttccaaa atgccaaat 
ttgagcgat tagttaaat tagctggca aacaattgg 
ctccagctg tttacaagc tgattactt atatcgagg 
agactcaga aggaagagc cagaatcgc cgtcgaaaa 
agtcaaaga aaaaagttg aatcgacca ctttgaaaa 
gcaaagtgc tgaaggaga cacgactta taccgaaag 
tagtttatg aaattgaac agtttggta aattagttg 
cgcagttgt ctaacaaga ctaaaattg atttggcca 
agttcatgg gtcagggga cagttcgag gttttaggt 
ttttgaatt gggaacaag ttgtggaga aggctaatg 
atggggcac cacaaacac aagatagtg aagaaaata 
atgaagaga gtgacgagg aaacgtcac cacctccgt 
caaaacaac gcaaaactt aattgttac gtttattaa 
tacaaaagt tattacaaa taaaattga aacttaatt 
acgatgaac aacccgtaa agttcttcc cttagaaac 
aacacttca aaactttgt atacaacat aacaccatg 
aggtatagt ctttctcct gatgattga ggtaaataa 
acctttatg ccacaaagt ccaaggggc ggttgaagc 
cccctttgc ggttcatgt actgtctgt atttgcgtt 
tcaagataa catgaaccc cacccacgt cattacctt 
caactttct tccctttgg tggtatcaa aactacaaa 
aacccatca acttcatca tttcctgtt catcggcac 
ttttaccct ccaagtctg cggcagtga caaccggtc 
tttccggct gcagcggtt ttgattttt tctcgacgg 
atctggacc tggacctcg tcaaggacc gggagtacc 
tccgtcggg gttccctct cgtgccgtc tttccctat 
ccctggacc tctccttcg accttttcc gtctgtggc 
gttctctct ctcgctccc tatccctgt cccgttccc 
tatcccgcc gccgagacc cgtagggcg atttcgagc 
gactccgac gtcccatca ctcacaaaa actcaaaaa 
aaaacaaat tttttccac aaaaatttt gacaactgt 
cacgatcga ttgagaaaa acgtcgatt ttagttatg 
attttttac acgaaagcg ctagtgttt caaaat
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