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Gene information
General information
TC012182 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3751421..3755461
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012182
      iBeetle screen iB_10498

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012182 RA
        MTYIDKLIS FHIVENENR KNFLSWTYP TITVDHEKY 
        ILSNCFARG VQNEYFCGR VQNNWIYIS QFVSSKTFA 
        IVIAAKEFE PNKYRQLCD ILGKKYAKS QNPVDLVTL 
        YLNLFTSGS CSLQENGAS FVCNFKNYE SVANLKEFI 
        ASFDLEIIL LYSAILLKR RILLFDNNM ELLLSELLA 
        ISNLVPLRK VADYLYPVI ESSNKLKGL SFYVAGTTN 
        SDLIRDEHL YDLFVNLRT REITVSPKA EEIMAMTKT 
        HKDIATFLV KLSASDLPE TQVKQQILQ KTKELLSLL 
        KSLASVTTD NHVKMVTMN DLRAKKYHP NLENFLFNL 
        AVSENMMIL
      CDS sequence
      • >TC012182 RA
        atgacatac attgataaa ttaatttcg tttcacatt 
        gttgaaaat gaaaatcgc aaaaacttt ctctcctgg 
        acctatccc acgataaca gtcgaccac gaaaaatac 
        atcctgtcg aattgtttc gcaagaggc gttcaaaac 
        gaatatttc tgtgggcgc gtccaaaat aactggatc 
        tacatcagc caattcgtg tcaagcaaa actttcgcg 
        attgtgata gcggcgaag gaatttgag ccaaacaag 
        taccgacag ttgtgtgat attttaggg aaaaaatac 
        gccaaaagc caaaatccg gtggatcta gttaccttg 
        tatttgaat ttgttcact tccggctcc tgctcgttg 
        caggaaaat ggtgcttcg tttgtttgc aatttcaaa 
        aattacgaa tccgttgcc aacttaaaa gagtttatc 
        gcatcgttt gatttggag ataattctg ctgtattcg 
        gcgatttta ttaaaaagg aggattttg ctgtttgat 
        aacaacatg gaactgctt ttaagtgaa ttgttggct 
        ataagcaat ttggttccg ctgagaaaa gtcgcagat 
        tatttatat ccagtaata gagagttca aataaatta 
        aaaggattg tcgttttat gtcgcaggg actacaaat 
        agtgactta atacgggat gaacacttg tatgacctc 
        ttcgttaat ctgcggaca cgggaaatt acagtatct 
        ccaaaagcc gaagagatc atggccatg accaaaacc 
        cacaaagac atagccact tttttggtg aaactgtcc 
        gcaagcgac ctacctgaa acccaagtc aaacaacaa 
        atcctccaa aagaccaaa gaattgctc agtttatta 
        aaaagcctg gcttcggta acaacagac aaccacgta 
        aagatggtc acgatgaat gacctaaga gctaagaaa 
        taccaccca aacttggaa aactttctc ttcaatttg 
        gctgtgtcc gaaaatatg atgattttg taa
      mRNA sequence
      • >TC012182 RA
        atgttcgaa attttacat tttcttgat cagaaacac 
        atgtagttg aagtggtcg taaagtata gtccatcat 
        aaattccaa ttacttaaa ttctgacaa atcaattta 
        atttttggt atcaggaac tcagaatca acacccgac 
        gtctcctta atgtttaca gcgatgtca gcacacttc 
        accatcatt catttaaat ggccttcaa ccaaattcc 
        tacttatct aaaccagca tttttatta gtcatgtgc 
        tcctagcct cacacaatg tttgtttac tccaaacga 
        tgacataca ttgataaat taatttcgt ttcacattg 
        ttgaaaatg aaaatcgca aaaactttc tctcctgga 
        cctatccca cgataacag tcgaccacg aaaaataca 
        tcctgtcga attgtttcg caagaggcg ttcaaaacg 
        aatatttct gtgggcgcg tccaaaata actggatct 
        acatcagcc aattcgtgt caagcaaaa ctttcgcga 
        ttgtgatag cggcgaagg aatttgagc caaacaagt 
        accgacagt tgtgtgata ttttaggga aaaaatacg 
        ccaaaagcc aaaatccgg tggatctag ttaccttgt 
        atttgaatt tgttcactt ccggctcct gctcgttgc 
        aggaaaatg gtgcttcgt ttgtttgca atttcaaaa 
        attacgaat ccgttgcca acttaaaag agtttatcg 
        catcgtttg atttggaga taattctgc tgtattcgg 
        cgattttat taaaaagga ggattttgc tgtttgata 
        acaacatgg aactgcttt taagtgaat tgttggcta 
        taagcaatt tggttccgc tgagaaaag tcgcagatt 
        atttatatc cagtaatag agagttcaa ataaattaa 
        aaggattgt cgttttatg tcgcaggga ctacaaata 
        gtgacttaa tacgggatg aacacttgt atgacctct 
        tcgttaatc tgcggacac gggaaatta cagtatctc 
        caaaagccg aagagatca tggccatga ccaaaaccc 
        acaaagaca tagccactt ttttggtga aactgtccg 
        caagcgacc tacctgaaa cccaagtca aacaacaaa 
        tcctccaaa agaccaaag aattgctca gtttattaa 
        aaagcctgg cttcggtaa caacagaca accacgtaa 
        agatggtca cgatgaatg acctaagag ctaagaaat 
        accacccaa acttggaaa actttctct tcaatttgg 
        ctgtgtccg aaaatatga tgattttgt aacttacgg 
        tgctttatt ctggtgcat caatttata aaataagta 
        taataaaaa tatacatca att
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY