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Gene information
General information
TC033801
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010019
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10129303..10130626
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC033801
      iBeetle screen iB_10497

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC033801 RA
        MPDTEDKPF GFGSAVKRF RPLGLHPEL ITVNTTNPL 
        GPGQYDPKP ACCKYKNNA ASWKVKLDS EEFSKYLGF 
        RNPQVLCDR MFERTLGGP GTYELFDYK EKGVSTLEN 
        VGFGSGERF HSAIRDETL PPGCHTLKT LTTKFTKTP 
        TFEFDGITS RFKNTEPPY RKAPNRYKL PTYCITNKV 
        VSKRGPYDT FTGPRDETT IKNHFAPPL FKSPDTFYA 
        IPSAVDHLL YHPSKKRNG KFLLEERFP EGLSVTATK 
        NGFSMFMRD PEYPSPAQY EVSQGIKAA KKSLHPFNS 
        SNVNARPPA NWKIHPGPD RYKVKYPTC HQKSVKGSW 
        MFLSTTERE LVKVESYSN FIY
      CDS sequence
      • >TC033801 RA
        atgcccgat acagaggac aaacccttt ggtttcggc 
        agcgctgtt aagcgcttt cgtcctttg gggcttcac 
        ccagaactg attaccgta aacacaaca aatcctctt 
        ggtcccgga caatacgat cctaaaccg gcttgttgt 
        aaatacaaa aataatgca gccagttgg aaggtaaaa 
        ttagacagt gaagaattt tccaagtat ttgggcttt 
        cgaaaccct caagtatta tgcgatcgc atgtttgaa 
        agaaccctt ggagggcca ggaacttac gaacttttt 
        gactataaa gaaaaagga gtttccact ttggaaaac 
        gttggtttt gggagtgga gaaagattt cactcggct 
        attagggat gaaacatta ccacctggt tgtcacact 
        ctcaaaact ttaacaaca aaatttaca aaaacacca 
        acttttgaa tttgatggt attacatcg aggttcaaa 
        aacactgaa cccccatat agaaaggcc ccaaatcgt 
        tacaaatta cccacttat tgcatcaca aataaagtt 
        gtttcaaaa agaggccct tatgacacg ttcaccgga 
        cctcgtgac gaaacaaca attaaaaac cactttgct 
        cctcctctt tttaaatca cctgacact ttttacgcc 
        atacctagt gcagttgac caccttctg taccacccg 
        agcaaaaaa aggaatggt aaattcttg ttggaagag 
        aggttcccg gaaggtttg tctgtaacc gcgaccaaa 
        aatgggttc agtatgttc atgagagat ccagaatat 
        ccaagtcct gcccaatat gaagtttca caagggatc 
        aaagcggcc aaaaaaagt ttgcatcct tttaacagc 
        tcaaacgtg aatgccaga cccccagca aactggaaa 
        attcatcca gggccggac cgctacaag gtaaaatat 
        cccacatgc caccagaag agcgtcaaa ggctcttgg 
        atgttcctg tcgacgacc gagcgcgag ttggtcaaa 
        gtggagagc tacagcaat tttatctac taa
      mRNA sequence
      • >TC033801 RA
        tgactcaaa tgaaaaatg atcaaaatg acataaaat 
        gtcacaata agtgtaaaa aatttaagc cgaaaaaat 
        gcccgatac agaggacaa accctttgg tttcggcag 
        cgctgttaa gcgctttcg tcctttggg gcttcaccc 
        agaactgat taccgtaaa cacaacaaa tcctcttgg 
        tcccggaca atacgatcc taaaccggc ttgttgtaa 
        atacaaaaa taatgcagc cagttggaa ggtaaaatt 
        agacagtga agaattttc caagtattt gggctttcg 
        aaaccctca agtattatg cgatcgcat gtttgaaag 
        aacccttgg agggccagg aacttacga actttttga 
        ctataaaga aaaaggagt ttccacttt ggaaaacgt 
        tggttttgg gagtggaga aagatttca ctcggctat 
        tagggatga aacattacc acctggttg tcacactct 
        caaaacttt aacaacaaa atttacaaa aacaccaac 
        ttttgaatt tgatggtat tacatcgag gttcaaaaa 
        cactgaacc cccatatag aaaggcccc aaatcgtta 
        caaattacc cacttattg catcacaaa taaagttgt 
        ttcaaaaag aggccctta tgacacgtt caccggacc 
        tcgtgacga aacaacaat taaaaacca ctttgctcc 
        tcctctttt taaatcacc tgacacttt ttacgccat 
        acctagtgc agttgacca ccttctgta ccacccgag 
        caaaaaaag gaatggtaa attcttgtt ggaagagag 
        gttcccgga aggtttgtc tgtaaccgc gaccaaaaa 
        tgggttcag tatgttcat gagagatcc agaatatcc 
        aagtcctgc ccaatatga agtttcaca agggatcaa 
        agcggccaa aaaaagttt gcatccttt taacagctc 
        aaacgtgaa tgccagacc cccagcaaa ctggaaaat 
        tcatccagg gccggaccg ctacaaggt aaaatatcc 
        cacatgcca ccagaagag cgtcaaagg ctcttggat 
        gttcctgtc gacgaccga gcgcgagtt ggtcaaagt 
        ggagagcta cagcaattt tatctacta aacacctct 
        gataaataa acgattagc acgttg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY