Status
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Gene information
General information
TC011044 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6179477..6183672
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011044
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10495

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011044 RA
      MTGRCKSKT RKPLETALG KAPKDSERA ADLAHLRKP 
      IETSVFGLK QVLSLLQTA TSEVREYIT YECDVLYEC 
      RTCRSIFRS LANFILHKR EYCREKFSL DGHHNQGGG 
      FVVAEDSQT LPPPPPPPP NENSSRCAT PEEKDPKKT 
      ILPIIDKLL KRQKIRLLA KDTLSEDLS KDENSDDDV 
      IFVSEENNV ILQKIDTSE AAVFQTVVK EGVKTEENE 
      GLIKSEVME IHGILENNE AVMDSTGKV CTFETSKPK 
      QELVCTECN FTFSTEKTL THHIKYKHN QTCIVYPCP 
      DCSETLSNA WSVYRHLYN VHRRTSAQI RRMRDLIHS 
      SRVRKSQKP PPKTPPEEE KQQRSESEE NQWMNNFER 
      DKDLQMCGG CGKRFERKA ALHSHSQMC TKRIALCNS 
      LKKGKAGPS DKRAEKGGA RGVQGAAKR KPALVSTYK 
      RTSEAKAPV GEVLTISDD SSEVPETLN KKRLKWDFD 
      PKLADISDE YKTYLNDET DHCFLAKAQ IYMDKAGLK 
      CIPCGSSFV SVYLLLRHM SVHFSWFRF QCSKCSFMS 
      FHKYDCTTH ASNQHSVPN AMMESTVLP IPKWKVLCS 
      SHDFEQVVE EMPPQLEKC EKSEAIVLE DDEMPVLLL 
      DDGKKGEEE EEKAGPSKG LENSRPVRN RTKSVKTVQ 
      DDFIYDLNN VLKLNDHSI KNRRMKKNL NK
    CDS sequence
    • >TC011044 RA
      atgacagga aggtgtaag agcaaaacg aggaagccg 
      ctggagaca gctctgggg aaggccccc aaagacagc 
      gagagagcg gccgacttg gcgcacttg cgcaagccg 
      atcgagaca agtgtgttt gggttgaag caagtcctg 
      tctttgctt caaaccgcc acgtctgaa gtcagggaa 
      tatataacg tacgagtgc gacgtcctg tacgaatgt 
      cgaacgtgt cggagtatt tttcggagt ttggctaat 
      tttattctg cacaagagg gaatactgt cgcgaaaaa 
      ttctcacta gatggtcac cacaaccag ggtggcggt 
      tttgttgtt gctgaagac agtcaaacg ttgccgccg 
      ccgccgccg ccaccgcca aatgaaaat tccagtaga 
      tgtgctaca cccgaagag aaagacccg aaaaagact 
      attttacca attattgat aaacttcta aagagacaa 
      aaaatcagg cttttggct aaagataca ctcagtgaa 
      gacttgtca aaagatgag aattcagat gatgacgtt 
      attttcgtt tcggaggaa aataacgtc attttgcag 
      aaaatcgat acttcagaa gctgccgtt ttccagact 
      gttgttaaa gaaggggtg aagacggag gaaaacgag 
      ggattgatt aaatcagag gtgatggaa atccacggc 
      attttggag aacaacgaa gccgtaatg gacagcacc 
      ggcaaagtg tgcaccttc gaaacctcc aaacccaag 
      caggagctc gtctgcacc gaatgcaac ttcaccttc 
      tccaccgag aaaaccctc acccaccac atcaagtac 
      aagcacaac caaacctgc atcgtctac ccttgtccc 
      gactgcagc gagacgctc tcgaacgcc tggagcgtc 
      taccgccac ttgtacaac gtccaccgg cgcacatcg 
      gcccagatc cggcgcatg cgcgacttg atccactcc 
      agccgcgtc cgcaagtcg caaaaaccc ccaccgaag 
      accccgccc gaagaagag aaacaacag cgcagcgaa 
      agcgaggaa aaccaatgg atgaacaat ttcgagcgc 
      gacaaggac ttgcaaatg tgcggcggc tgtggaaag 
      cggttcgaa cgcaaagcc gccttgcat tcgcactcg 
      caaatgtgc accaagaga atcgcgctg tgcaacagc 
      cttaagaaa ggcaaggcg gggccaagt gacaagagg 
      gctgagaag ggcggagct aggggtgta caaggggcg 
      gcgaagcgc aaaccggcg ctggtctcg acctacaag 
      cgcacaagc gaagctaaa gcgccagta ggcgaggtt 
      ttgaccatt tcggacgat tcgagcgaa gttccagaa 
      actttgaac aagaagcgc ctcaagtgg gattttgac 
      ccgaaactg gccgacatt tcggacgaa tacaagacg 
      tatctgaac gacgaaacc gaccattgt ttcctggcc 
      aaggcgcaa atttacatg gataaagcc gggcttaag 
      tgcatccct tgcgggagc agttttgtg tcagtctat 
      ctcttgctg aggcacatg tctgtgcat ttctcctgg 
      tttaggttc caatgtagc aagtgttcg ttcatgtcg 
      tttcataaa tacgactgt actacgcat gctagcaac 
      cagcacagt gttcccaat gcgatgatg gagagtaca 
      gtgttgcca attcccaag tggaaggtt ttgtgttcg 
      tcgcatgat ttcgagcaa gttgtggag gaaatgcct 
      ccacagttg gagaagtgt gagaagagt gaggcgatt 
      gttttggag gatgatgag atgccggtg cttttattg 
      gacgatggg aagaagggg gaggaggag gaggagaag 
      gcggggcct tcgaaaggt ttggaaaat agtcggccg 
      gtccgaaat cggacgaaa agtgtgaag acggtgcaa 
      gacgatttt atttatgat ttgaacaat gttttgaaa 
      cttaacgat cattccatt aagaataga agaatgaaa 
      aagaatcta aataagtaa
    mRNA sequence
    • >TC011044 RA
      aagatctgg caaaaatcg cccgagaat acgcaaaaa 
      cagccccat ttctcacaa tgacaggaa ggtgtaaga 
      gcaaaacga ggaagccgc tggagacag ctctgggga 
      aggccccca aagacagcg agagagcgg ccgacttgg 
      cgcacttgc gcaagccga tcgagacaa gtgtgtttg 
      ggttgaagc aagtcctgt ctttgcttc aaaccgcca 
      cgtctgaag tcagggaat atataacgt acgagtgcg 
      acgtcctgt acgaatgtc gaacgtgtc ggagtattt 
      ttcggagtt tggctaatt ttattctgc acaagaggg 
      aatactgtc gcgaaaaat tctcactag atggtcacc 
      acaaccagg gtggcggtt ttgttgttg ctgaagaca 
      gtcaaacgt tgccgccgc cgccgccgc caccgccaa 
      atgaaaatt ccagtagat gtgctacac ccgaagaga 
      aagacccga aaaagacta ttttaccaa ttattgata 
      aacttctaa agagacaaa aaatcaggc ttttggcta 
      aagatacac tcagtgaag acttgtcaa aagatgaga 
      attcagatg atgacgtta ttttcgttt cggaggaaa 
      ataacgtca ttttgcaga aaatcgata cttcagaag 
      ctgccgttt tccagactg ttgttaaag aaggggtga 
      agacggagg aaaacgagg gattgatta aatcagagg 
      tgatggaaa tccacggca ttttggaga acaacgaag 
      ccgtaatgg acagcaccg gcaaagtgt gcaccttcg 
      aaacctcca aacccaagc aggagctcg tctgcaccg 
      aatgcaact tcaccttct ccaccgaga aaaccctca 
      cccaccaca tcaagtaca agcacaacc aaacctgca 
      tcgtctacc cttgtcccg actgcagcg agacgctct 
      cgaacgcct ggagcgtct accgccact tgtacaacg 
      tccaccggc gcacatcgg cccagatcc ggcgcatgc 
      gcgacttga tccactcca gccgcgtcc gcaagtcgc 
      aaaaacccc caccgaaga ccccgcccg aagaagaga 
      aacaacagc gcagcgaaa gcgaggaaa accaatgga 
      tgaacaatt tcgagcgcg acaaggact tgcaaatgt 
      gcggcggct gtggaaagc ggttcgaac gcaaagccg 
      ccttgcatt cgcactcgc aaatgtgca ccaagagaa 
      tcgcgctgt gcaacagcc ttaagaaag gcaaggcgg 
      ggccaagtg acaagaggg ctgagaagg gcggagcta 
      ggggtgtac aaggggcgg cgaagcgca aaccggcgc 
      tggtctcga cctacaagc gcacaagcg aagctaaag 
      cgccagtag gcgaggttt tgaccattt cggacgatt 
      cgagcgaag ttccagaaa ctttgaaca agaagcgcc 
      tcaagtggg attttgacc cgaaactgg ccgacattt 
      cggacgaat acaagacgt atctgaacg acgaaaccg 
      accattgtt tcctggcca aggcgcaaa tttacatgg 
      ataaagccg ggcttaagt gcatccctt gcgggagca 
      gttttgtgt cagtctatc tcttgctga ggcacatgt 
      ctgtgcatt tctcctggt ttaggttcc aatgtagca 
      agtgttcgt tcatgtcgt ttcataaat acgactgta 
      ctacgcatg ctagcaacc agcacagtg ttcccaatg 
      cgatgatgg agagtacag tgttgccaa ttcccaagt 
      ggaaggttt tgtgttcgt cgcatgatt tcgagcaag 
      ttgtggagg aaatgcctc cacagttgg agaagtgtg 
      agaagagtg aggcgattg ttttggagg atgatgaga 
      tgccggtgc ttttattgg acgatggga agaaggggg 
      aggaggagg aggagaagg cggggcctt cgaaaggtt 
      tggaaaata gtcggccgg tccgaaatc ggacgaaaa 
      gtgtgaaga cggtgcaag acgatttta tttatgatt 
      tgaacaatg ttttgaaac ttaacgatc attccatta 
      agaatagaa gaatgaaaa agaatctaa ataagtaag 
      ttgcatgtg tatacctaa aggaaaata aagtgttat 
      ttacaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY