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Gene information
General information
TC009883 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12716829..12722671
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009883
    iBeetle screen iB_10494

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009883 RA
      MVLIWTKFS VLILAGLAN GVIRQGLNG QWKVTGGPN 
      KTLTIDAYV PGGIFSALM ESGTIGDIF YGTGDSNYS 
      WVGLTNWTY STTFTVSEE LLNSRVVLV VFEGLDTFA 
      TVSVNGHEV GTSENMFVR YNFNIRDQL QVGANEISV 
      QFASAVATA EALAKDPSN EYVIPPECP VPEYKGICY 
      VNRIRKQQA SFAWDWGPA FASVGIWGL TEIIGFDDA 
      FIEYLVATP LKNPDDDFW TVPLSVYLN AGEIAAISG 
      TIDVSLETE KGVRTSSFK FNETVDEFE VKVTTTLLV 
      PSSEVSTWW PNGYGTQST YKLLVTFVS EHERDQSTV 
      HIGFRTVEL VQEKLGTGQ GLSFYYKVN QVPIFAKGA 
      NAIPINILP ERSQSLNLA PLFETMKSA HMNMLRVWG 
      GGVYNVDYF YSLADDAGI MIWQDFMFA CALYPTDDA 
      YLKNVVKEV KHQVRRLAS HPSVVIWAG NNENEAVLA 
      QDWYDTMDN FTIYKNDYV KLYVDVIRP EVLKIVPNA 
      TYLTSSPTN GLESDQEGY VAQNPGDSL YGDVHFYDY 
      TSDSWAPET FPIPRFASE YGYQSMPSI ESWLTATAS 
      LDDLRPDSA FMDYRQHHP GGNDQNVDL IELQMQLPD 
      PESENYVKA FIYFSQILQ AQAMKIQTE HYRSFKGRV 
      NEKGEGNTM GALYWQLND VWVAPTWSG IDFTGRWKM 
      LQYFAKDFF NPMIITGRL TDDNLEVYA ICDGLELPN 
      VTVKGLVQV YKWESLEPL IAVESPISL DLYKSVPVT 
      TIHAPTALQ TAGCGSDYA EAKKKCFFH LRLLDQGNN 
      ILAPFNYIF PEKLKNCAL AKPAVKIES VEIVDLYKK 
      IFSVTVSTD AVALFVWLD THEVKGVFS ENGFLQVLP 
      SKTVNFTAD SRVTLEELQ AAVTVTHLS DPAYL
    CDS sequence
    • >TC009883 RA
      atggtgtta atttggaca aagttttct gtgttaata 
      ttggccggt ttggccaat ggggtgatt cggcaaggc 
      ctcaacggc caatggaaa gtgacaggg gggcccaac 
      aaaacttta actattgat gcctatgtt cccggcggc 
      atattttcc gccctcatg gaaagtggc acaattggg 
      gatattttc tacggtact ggggactca aattacagt 
      tgggtgggc ctaaccaac tggacttac agcacaaca 
      ttcacagtc agcgaagaa ttgctaaac agtcgagtt 
      gtcttagtg gtttttgag ggtttggac acatttgcg 
      actgtttcg gtcaatgga cacgaagtg gggacgagc 
      gagaacatg tttgttcgc tacaatttc aacattaga 
      gaccagctt caagtgggt gcgaatgaa atttcggtt 
      cagttcgcc tctgcggtc gccacggcc gaagccttg 
      gccaaggac ccaagcaac gaatacgta atcccgccc 
      gaatgccct gttcccgag tacaaaggc atttgctac 
      gttaatcgt atcagaaaa cagcaagct tcgttcgcg 
      tgggattgg ggccccgct tttgcctct gtgggaata 
      tggggtttg actgaaata atcggtttc gatgacgct 
      tttattgaa tatttggtg gcaacaccg ttgaaaaat 
      ccagacgat gatttctgg actgttccc ctttctgtg 
      tacctaaat gcaggcgaa atcgctgcg atttcaggg 
      acaatagat gtttctttg gaaacagag aagggtgta 
      aggacctcc agttttaaa tttaacgaa actgtggac 
      gagtttgaa gtcaaagtg acgactacc ctactggtg 
      ccatccagt gaggtgagc acgtggtgg cctaacggc 
      tacggcacg cagtccacg tacaaattg ttggtaaca 
      tttgttagc gagcacgag cgcgaccaa agcactgtg 
      cacataggg ttcagaacg gtggagcta gtccaggaa 
      aaattaggt acaggccaa ggcctcagc ttttattac 
      aaagtcaac caagtcccg attttcgca aagggggcc 
      aacgcaatc cccattaac attcttccg gagcggagc 
      caaagcctc aacctagct ccgcttttt gaaaccatg 
      aaaagtgcc cacatgaac atgttgcgc gtgtggggc 
      ggaggcgtt tacaacgtg gattatttt tacagcctt 
      gccgatgat gctgggata atgatttgg caggatttc 
      atgtttgcg tgcgctttg tatcccact gacgatgct 
      tatttgaag aacgtggtt aaggaagtt aaacatcaa 
      gtgagacgc cttgccagc caccctagt gtggtgatt 
      tgggccgga aacaacgaa aatgaggcc gttttagcg 
      caagattgg tacgacacc atggacaat ttcacgatt 
      tataaaaat gattacgtt aagttgtac gttgatgtt 
      attcgacct gaagtcctt aaaatcgta cccaacgca 
      acttactta acttcaagc cctactaac gggctagaa 
      agcgatcag gagggttac gtggcccag aatccgggg 
      gattccctc tacggagac gttcacttt tacgattac 
      acaagcgac agttgggcc cctgagact ttcccaatc 
      ccgcggttt gcctcagaa tacggctac caatcaatg 
      cccagcatc gagtcctgg cttactgcc accgccagt 
      ttggatgat ttaagacct gatagtgcc ttcatggac 
      tatcggcaa caccaccct ggcggtaac gaccaaaac 
      gtcgattta attgaatta cagatgcaa ctgcctgat 
      cctgagagc gaaaactac gttaaagct ttcatttat 
      ttctctcaa attctccaa gcacaggcg atgaaaatc 
      caaactgag cactatcgc agtttcaag gggcgagtc 
      aatgagaag ggggagggt aacaccatg ggggccctc 
      tactggcag ctaaacgac gtctgggtg gcacccaca 
      tggtctggg atagatttt accgggcgg tggaaaatg 
      ttacagtac ttcgccaaa gatttcttc aaccccatg 
      attatcaca gggagactc acggacgat aatttggag 
      gtttatgcc atttgtgac ggtctagaa ctaccaaat 
      gtaacagtt aaagggtta gttcaggtt tataagtgg 
      gagtctcta gagccgcta attgcagta gaatcgcca 
      atttctctg gatttgtac aaatcggtc cctgtcaca 
      accatccat gcgcccacc gctttacag accgccggt 
      tgtggcagc gattatgcc gaagcgaag aaaaaatgt 
      ttcttccat cttcggttg ctcgaccag ggtaataat 
      attttggcc ccgtttaat tatatcttc ccggagaaa 
      ctcaaaaac tgtgccctc gccaagcct gcagtgaag 
      attgagtcc gttgagatt gtcgatttg tacaagaaa 
      attttcagc gtgactgtg tccacagat gcggtcgct 
      ttgttcgtg tggttggac actcatgaa gtcaaagga 
      gtgttttcg gaaaacggc tttttgcaa gtgcttccc 
      agcaaaact gttaatttt acagccgac agtcgcgtc 
      acgctggaa gagttgcaa gccgctgtt acagttaca 
      catttaagc gatcccgcg tatctgtaa
    mRNA sequence
    • >TC009883 RA
      cacagtaac ttactgcgt caccatggt gttaatttg 
      gacaaagtt ttctgtgtt aatattggc cggtttggc 
      caatggggt gattcggca aggcctcaa cggccaatg 
      gaaagtgac aggggggcc caacaaaac tttaactat 
      tgatgccta tgttcccgg cggcatatt ttccgccct 
      catggaaag tggcacaat tggggatat tttctacgg 
      tactgggga ctcaaatta cagttgggt gggcctaac 
      caactggac ttacagcac aacattcac agtcagcga 
      agaattgct aaacagtcg agttgtctt agtggtttt 
      tgagggttt ggacacatt tgcgactgt ttcggtcaa 
      tggacacga agtggggac gagcgagaa catgtttgt 
      tcgctacaa tttcaacat tagagacca gcttcaagt 
      gggtgcgaa tgaaatttc ggttcagtt cgcctctgc 
      ggtcgccac ggccgaagc cttggccaa ggacccaag 
      caacgaata cgtaatccc gcccgaatg ccctgttcc 
      cgagtacaa aggcatttg ctacgttaa tcgtatcag 
      aaaacagca agcttcgtt cgcgtggga ttggggccc 
      cgcttttgc ctctgtggg aatatgggg tttgactga 
      aataatcgg tttcgatga cgcttttat tgaatattt 
      ggtggcaac accgttgaa aaatccaga cgatgattt 
      ctggactgt tcccctttc tgtgtacct aaatgcagg 
      cgaaatcgc tgcgatttc agggacaat agatgtttc 
      tttggaaac agagaaggg tgtaaggac ctccagttt 
      taaatttaa cgaaactgt ggacgagtt tgaagtcaa 
      agtgacgac taccctact ggtgccatc cagtgaggt 
      gagcacgtg gtggcctaa cggctacgg cacgcagtc 
      cacgtacaa attgttggt aacatttgt tagcgagca 
      cgagcgcga ccaaagcac tgtgcacat agggttcag 
      aacggtgga gctagtcca ggaaaaatt aggtacagg 
      ccaaggcct cagctttta ttacaaagt caaccaagt 
      cccgatttt cgcaaaggg ggccaacgc aatccccat 
      taacattct tccggagcg gagccaaag cctcaacct 
      agctccgct ttttgaaac catgaaaag tgcccacat 
      gaacatgtt gcgcgtgtg gggcggagg cgtttacaa 
      cgtggatta tttttacag ccttgccga tgatgctgg 
      gataatgat ttggcagga tttcatgtt tgcgtgcgc 
      tttgtatcc cactgacga tgcttattt gaagaacgt 
      ggttaagga agttaaaca tcaagtgag acgccttgc 
      cagccaccc tagtgtggt gatttgggc cggaaacaa 
      cgaaaatga ggccgtttt agcgcaaga ttggtacga 
      caccatgga caatttcac gatttataa aaatgatta 
      cgttaagtt gtacgttga tgttattcg acctgaagt 
      ccttaaaat cgtacccaa cgcaactta cttaacttc 
      aagccctac taacgggct agaaagcga tcaggaggg 
      ttacgtggc ccagaatcc gggggattc cctctacgg 
      agacgttca cttttacga ttacacaag cgacagttg 
      ggcccctga gactttccc aatcccgcg gtttgcctc 
      agaatacgg ctaccaatc aatgcccag catcgagtc 
      ctggcttac tgccaccgc cagtttgga tgatttaag 
      acctgatag tgccttcat ggactatcg gcaacacca 
      ccctggcgg taacgacca aaacgtcga tttaattga 
      attacagat gcaactgcc tgatcctga gagcgaaaa 
      ctacgttaa agctttcat ttatttctc tcaaattct 
      ccaagcaca ggcgatgaa aatccaaac tgagcacta 
      tcgcagttt caaggggcg agtcaatga gaaggggga 
      gggtaacac catgggggc cctctactg gcagctaaa 
      cgacgtctg ggtggcacc cacatggtc tgggataga 
      ttttaccgg gcggtggaa aatgttaca gtacttcgc 
      caaagattt cttcaaccc catgattat cacagggag 
      actcacgga cgataattt ggaggttta tgccatttg 
      tgacggtct agaactacc aaatgtaac agttaaagg 
      gttagttca ggtttataa gtgggagtc tctagagcc 
      gctaattgc agtagaatc gccaatttc tctggattt 
      gtacaaatc ggtccctgt cacaaccat ccatgcgcc 
      caccgcttt acagaccgc cggttgtgg cagcgatta 
      tgccgaagc gaagaaaaa atgtttctt ccatcttcg 
      gttgctcga ccagggtaa taatatttt ggccccgtt 
      taattatat cttcccgga gaaactcaa aaactgtgc 
      cctcgccaa gcctgcagt gaagattga gtccgttga 
      gattgtcga tttgtacaa gaaaatttt cagcgtgac 
      tgtgtccac agatgcggt cgctttgtt cgtgtggtt 
      ggacactca tgaagtcaa aggagtgtt ttcggaaaa 
      cggcttttt gcaagtgct tcccagcaa aactgttaa 
      ttttacagc cgacagtcg cgtcacgct ggaagagtt 
      gcaagccgc tgttacagt tacacattt aagcgatcc 
      cgcgtatct gtaaatgtg aatgaaata aaggtttaa 
      acaagagat acgaatata tttatttta tattgatac 
      aaatagtat acaccaaaa catccaact aatctacga 
      actgttcaa attaccgtt aaataaatt tgtacttga 
      taaaataac agctcttac aaggattta acaataact 
      tgcaaaaat aatttgcaa gagtatccc aaactttat 
      tcactttac aaaatgagc ttaccaata ttcagactt 
      ttagtcagt taatttgta aatatgtat gtacattag 
      tcgtttatt tcttgtatc taaccttaa taaaatcat 
      tgcgtacaa ctaaaacct taaagtaat ttcactgaa 
      acttgcata aaaaagctc ggacttttt taagcgaaa 
      cggaaagga agagatcaa tgtattaac aataagtca 
      acgtaaaac tagagtttg tgatgcttg ttattaagg 
      caagcgttc tcgtttacg tttttcact attatcaaa 
      aataaattc accgaaagc tgcgttaaa gaaattagg 
      tcaagcaga gtgaaacta aaaaaaact agcagaatc 
      agtataaac tgttatatt tttcagatt gttccttta 
      aaaaactac aagctatca tggtgtaaa agctaggca 
      caggtaata ggctggggg taaaaaccg cctcgtact 
      atcagtgca tcgaactgt acctccaaa tgcacgatc 
      accggtgtg aatcgaaaa caactatag ctggttcaa 
      gcgtaggta ggtacaaaa atacattaa ttcgggctt 
      cgataatta aaaggtact ctacaatag tccacaaat 
      aattaagac ccacccgct atcatcatc ctcgacgat 
      ctgcaccca ctattcaca ttgtacaac ttataaaaa 
      aatataaca tttcgtaag aactatcga gggtgtcgt 
      tgcgataat tatttttgg actaccctc acattaatt 
      acacacaaa catcgaatt ttcaccaat ttcacgatt 
      tccgaacaa aaacaaagg aaaagaaaa caacatgat 
      ttctaaaat cataaaaca atcaattag cgccactat 
      attccaaaa gaataatat aaatttatc acagacaat 
      cttgtataa aattttcct cgtccatcc attaaacat 
      cagctattg tttgaaaac aaccgaaaa caaaaacaa 
      taaaaagcc taaaattag aaagatttg cggaactac 
      aatcgacac caacaccag ttccacgga cccaataca 
      cttaaagcc gaaaagaaa acaatcaac atatattgc 
      acattttta agatacgta tccaaaatg ttacacggc 
      ttatgccac atcgaaaca gcaattata tacccttgt 
      taatatact tagtacaca acggcaggt cagaatgtg 
      caaaattac ccaacaact taaagaaaa aacgaacgg 
      aacgctctg aaagagcca ttttaggtt tctttctct 
      aagagaaca cacaattat ttcagccac aaacaactt 
      ttataaaaa taaagtgac tgtgttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY