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Gene information
General information
TC006621 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12062901..12066136
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006621
    iBeetle screen iB_10492

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006621 RA
      MKLYCLSND PNKPCHILT FKEITLMLD CGLSMQSVL 
      NFLPLSFVA SSKLSNLPN WMPNDVSDS DLEGELKEN 
      GDRIFVDSP PEFCPPLNK LIDFAQVDV ILISNYLCM 
      MALPFITEG TGFQGRVYA TEPTLQIGR LLLEELVNY 
      IDQCPKASF AAQWKNLIH KLPYPLSDS FKPKSWKQL 
      YNMNDVNAS LSRIQMVGY NEKIDVYGA LKVMPSSSG 
      FCLGSSNWI ITSDHEKIV YLSGSSTLT THPRPMDHH 
      SLKNADVLI MTDLTQTPI SNPDSMLGV LCVVVGLTL 
      RGGGNVLIP CYPTGVVYD LFECLSVKM QDLGVSNCP 
      MFFVSPVAD TSLAYSNIL AEWLSSVKQ NKVYVTEEP 
      FPHGLLVKN NKLKHFKHV YSDGFSTDF QEPCVVFCG 
      HPSLRFGDV VRFIELWGN NPRNCIVFT EPDFDYTEA 
      LAPYQPLQM KVAHCAIDT SLNFTQANK LIRDLKPNT 
      LVVPECYTQ PPVSAPNLT DYVIENNHD RTLIPYKWG 
      EVINLPLKR KHSQIFIET GVAQKIVPV EVKPGISLS 
      SITGTLSIK DNVHSIQDL SSSVNKASK HVKYEWGSL 
      NVTDFLQKL SQEGISEPK VESHGNVVV IHLQEEDAL 
      IQLEDNSTH VVCTGDETL RTKLRSIVM QCLKKF
    CDS sequence
    • >TC006621 RA
      atgaaatta tactgtttg agtaacgac ccgaacaag 
      ccatgtcac atccttacc ttcaaggaa ataacttta 
      atgttggac tgcggtctt tccatgcaa tcagtatta 
      aatttcctc cctttatca tttgttgcc tcgagtaaa 
      ttatcaaac ttaccaaac tggatgcca aacgacgtc 
      tctgattca gatttggaa ggagaattg aaagagaat 
      ggggaccgc atttttgtc gactcaccg ccggaattt 
      tgcccccct ttaaacaaa ctcatagat tttgcccaa 
      gtcgacgtt atcttaatt tcaaattat ttgtgcatg 
      atggcactg ccatttatc acagaaggg acagggttc 
      caaggtagg gtgtacgcc actgagccc actctccaa 
      atcggacga ctactactc gaggaactc gttaattac 
      attgaccag tgccccaaa gccagtttt gcagcccag 
      tggaaaaat ctcattcat aaattaccc tatcctttg 
      agcgattcc ttcaagccc aaatcctgg aaacaactc 
      tacaacatg aacgacgtc aatgcgagt ttatcgcgc 
      atacagatg gtggggtac aacgagaaa attgacgtt 
      tacggggct ttgaaagtg atgccttca agctccggc 
      ttttgcctc ggctcctcc aactggatc atcacctcc 
      gaccacgaa aagatcgtc tatttgagc ggctcaagc 
      accctcacc acccacccc cgccccatg gaccaccac 
      tccctcaaa aacgccgat gtcttgatc atgacagac 
      ctcacacaa acccccatc agtaacccg gactcgatg 
      ttgggcgtt ttgtgcgtg gtggtgggg ctcacgcta 
      cgtggggga ggcaatgtt ttaatcccg tgctatccc 
      acgggagtt gtctacgat ttatttgag tgcttgtcg 
      gtgaaaatg caagatttg ggcgtttcg aactgtccc 
      atgtttttc gtgtctcca gtggctgat actagccta 
      gcctactct aatatttta gccgagtgg ctttcgtcg 
      gttaaacag aacaaggtg tatgtgacc gaagagccg 
      ttcccgcac ggacttttg gtcaaaaac aacaaactt 
      aagcatttt aaacatgtt tactcggac gggttcagc 
      acggatttc caggagccc tgtgttgtt ttttgtggg 
      cacccctcc ttaagattt ggcgatgtt gtacgtttt 
      atcgagttg tggggcaat aacccaagg aactgcatt 
      gtttttaca gagcctgat ttcgactac accgaagcc 
      ttagccccg taccagccc ctgcaaatg aaagtggct 
      cactgtgcc atcgacact tcgctcaat ttcacccaa 
      gccaacaaa ctcatcagg gacttaaaa ccgaacaca 
      cttgtggtc cctgaatgc tacactcag cccccagtt 
      tcggcccca aacctgacc gactatgtt attgaaaat 
      aatcacgac cgtacatta attccgtac aaatggggc 
      gaagtgatt aacttgcct ttgaagcgg aaacatagt 
      cagattttt attgaaacc ggagttgct caaaaaatt 
      gtgccggtg gaagtgaag cctgggatt agtttatcg 
      agtattacg ggcactttg agtattaaa gacaacgtg 
      cacagcata caggatttg tcgtccagt gtgaataag 
      gcgagtaaa cacgtcaag tatgagtgg gggagtttg 
      aatgtgact gattttttg caaaagttg agtcaggag 
      ggaattagt gagccgaag gtggagtcg catgggaat 
      gtcgttgtt atacatttg caagaggaa gatgcgttg 
      attcagttg gaagacaat agtacgcac gtggtgtgc 
      actggtgac gaaactttg aggacaaag ctgcgcagt 
      atcgttatg cagtgtttg aagaagttt tag
    mRNA sequence
    • >TC006621 RA
      gattgataa aaagattaa aaagtaatt attaattaa 
      ttaagagac caaagtgaa gttttttgt ccccaatcg 
      tttgtggaa tgtgggttg catatcaat tatttttta 
      tgacagttg tggttatgt tcgtttttt gtcggcgtt 
      ttaggttat aaacagttt taaaatgaa attatactg 
      tttgagtaa cgacccgaa caagccatg tcacatcct 
      taccttcaa ggaaataac tttaatgtt ggactgcgg 
      tctttccat gcaatcagt attaaattt cctcccttt 
      atcatttgt tgcctcgag taaattatc aaacttacc 
      aaactggat gccaaacga cgtctctga ttcagattt 
      ggaaggaga attgaaaga gaatgggga ccgcatttt 
      tgtcgactc accgccgga attttgccc ccctttaaa 
      caaactcat agattttgc ccaagtcga cgttatctt 
      aatttcaaa ttatttgtg catgatggc actgccatt 
      tatcacaga agggacagg gttccaagg tagggtgta 
      cgccactga gcccactct ccaaatcgg acgactact 
      actcgagga actcgttaa ttacattga ccagtgccc 
      caaagccag ttttgcagc ccagtggaa aaatctcat 
      tcataaatt accctatcc tttgagcga ttccttcaa 
      gcccaaatc ctggaaaca actctacaa catgaacga 
      cgtcaatgc gagtttatc gcgcataca gatggtggg 
      gtacaacga gaaaattga cgtttacgg ggctttgaa 
      agtgatgcc ttcaagctc cggcttttg cctcggctc 
      ctccaactg gatcatcac ctccgacca cgaaaagat 
      cgtctattt gagcggctc aagcaccct caccaccca 
      cccccgccc catggacca ccactccct caaaaacgc 
      cgatgtctt gatcatgac agacctcac acaaacccc 
      catcagtaa cccggactc gatgttggg cgttttgtg 
      cgtggtggt ggggctcac gctacgtgg gggaggcaa 
      tgttttaat cccgtgcta tcccacggg agttgtcta 
      cgatttatt tgagtgctt gtcggtgaa aatgcaaga 
      tttgggcgt ttcgaactg tcccatgtt tttcgtgtc 
      tccagtggc tgatactag cctagccta ctctaatat 
      tttagccga gtggctttc gtcggttaa acagaacaa 
      ggtgtatgt gaccgaaga gccgttccc gcacggact 
      tttggtcaa aaacaacaa acttaagca ttttaaaca 
      tgtttactc ggacgggtt cagcacgga tttccagga 
      gccctgtgt tgttttttg tgggcaccc ctccttaag 
      atttggcga tgttgtacg ttttatcga gttgtgggg 
      caataaccc aaggaactg cattgtttt tacagagcc 
      tgatttcga ctacaccga agccttagc cccgtacca 
      gcccctgca aatgaaagt ggctcactg tgccatcga 
      cacttcgct caatttcac ccaagccaa caaactcat 
      cagggactt aaaaccgaa cacacttgt ggtccctga 
      atgctacac tcagccccc agtttcggc cccaaacct 
      gaccgacta tgttattga aaataatca cgaccgtac 
      attaattcc gtacaaatg gggcgaagt gattaactt 
      gcctttgaa gcggaaaca tagtcagat ttttattga 
      aaccggagt tgctcaaaa aattgtgcc ggtggaagt 
      gaagcctgg gattagttt atcgagtat tacgggcac 
      tttgagtat taaagacaa cgtgcacag catacagga 
      tttgtcgtc cagtgtgaa taaggcgag taaacacgt 
      caagtatga gtgggggag tttgaatgt gactgattt 
      tttgcaaaa gttgagtca ggagggaat tagtgagcc 
      gaaggtgga gtcgcatgg gaatgtcgt tgttataca 
      tttgcaaga ggaagatgc gttgattca gttggaaga 
      caatagtac gcacgtggt gtgcactgg tgacgaaac 
      tttgaggac aaagctgcg cagtatcgt tatgcagtg 
      tttgaagaa gttttagtc aggcgttta aacaattta 
      ttacattga atatcgtat ttcacatgt atataatat 
      gtatgcaga agtagacaa agatgaaaa tacttgtat 
      ttaatactt gcacgtgtt tttccaaac accctgcac 
      ttatcaaac acaaagtta caattgtaa attgtacaa 
      cagcttacg agataatat tacagtaaa aagtaataa 
      atatagtat attttacag acatgtata tatggtgga 
      agaaacaaa cgcacactt tacctcttg attgtctta 
      aaaaaaagt aattacaaa tatatcaaa aactacaca 
      cgagagtaa cttatgaga aatttacac agtgtatat 
      attaaataa tacaggcac gttttttga tatttctgg 
      ataaagtaa aacacatgg aatgcttaa taaacttat 
      tcaaataat tcacagctt tcttcataa agtatgtac 
      aaaattgac tgataaaag acagccaaa tcacatttc 
      ttgaacatt gtaaggtct gtctaacgt atttaagca 
      aaatcaccc tgcctggtt tcgtgtcat acagtgaga 
      aggtttaaa tgttcagtg gtttagtac agtaggtaa 
      cagtaaagg gcaggcagc gccaccgcg ttatatttt 
      ttttatttc aataaataa attaaatat taatttatt 
      gcgacagac tatcagtgt ccactagat tataaaaaa 
      aagactaag aaagtttca acaatattg aggctttcc 
      agattttta tgaatcaaa tgtaattct caacaaccg 
      tttttaaaa aataaataa ttttttatt aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY