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Gene information
General information
TC014996 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4874374..4876848
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014996
    iBeetle screen iB_10488

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Adult morphology
    odoriferous glands darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    odoriferous glands necrotic - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection


    abdominal odoriferous glands colour darker - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    abdominal odoriferous glands content necrotic - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    abdominal odoriferous glands content not present - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014996 RA
      MARRPPPSH RIRVENEMI RRREKEFCH DRLWNGQRR 
      YYEDFERKN TKYDEWTSP RYYENQQKL MEKTKKERE 
      HEENLERRR AKLKKLLYD EEKLQEAEL QIRKVRNMT 
      SVRSPRPNE IPTEILKEL NNGLKLEEE ERRRHQAEL 
      KLYHQWRSS NPFLNHYER MQASRDLKL SWLDQQIEN 
      RMKKEKEEE ECRKLLKER EKIMKEEQE KFDQEQKRL 
      QLKKEELKA SLEQQIDEM RLKTQISED LKRKEDEER 
      RKKAELDEI ALKRIEEEK KRLERECAL DNIKQHKLK 
      LKKKAEDVV KTLDEERYL IMKLKELEI AERLEDEMK 
      KIEVKAALD QFLALNEEQ KKLEKIRQK NLDFLFDSE 
      AKTMYEQQE KFWKEEEAS RAKLLKDVI DTIQGQIQS 
      NIEKNRRRQ LEVIQEREE MLKKIEEHN QEMARLKEE 
      EETKKRKMM SMLKNDLEM KNLKKKAKE NAELRRIDE 
      ELERVRKEE ERLKQEILN IQRRQGPIK STRSKSFFC
    CDS sequence
    • >TC014996 RA
      atggcacga aggccaccc cctagccac cgaatccgc 
      gtggaaaat gagatgatc agacggcgg gaaaaagag 
      ttttgtcac gaccgtcta tggaacggt caaagacgt 
      tactacgaa gattttgaa aggaagaat acaaaatat 
      gatgaatgg acgtcaccc cgatattac gaaaatcag 
      caaaaactt atggaaaaa acgaagaaa gaacgcgaa 
      catgaggaa aatcttgag cggaggaga gcaaaattg 
      aagaaattg ctttatgac gaagaaaag ttacaagaa 
      gctgaattg cagattcgc aaggtcaga aatatgacc 
      tctgttagg tcgccacga cctaacgag ataccaact 
      gaaattttg aaggagctc aataatggt ttgaaattg 
      gaggaagaa gaaagaaga agacaccaa gcggaactc 
      aagttgtat caccaatgg aggagtagt aaccccttc 
      ttaaatcat tacgaaagg atgcaagcc agtcgggat 
      ttaaaattg tcttggttg gaccaacaa atcgaaaat 
      cggatgaag aaggagaag gaagaggaa gaatgtcgc 
      aagttgcta aaagaacgc gagaaaatc atgaaagaa 
      gaacaggag aagtttgat caagaacag aaaaggtta 
      caattgaag aaggaagaa ttaaaagcg agtctggaa 
      caacaaatt gacgagatg cgtctaaag acgcaaatt 
      agtgaagat ctgaaacga aaagaagat gaagaaagg 
      aggaagaaa gccgaattg gatgaaatc gcactgaag 
      cggattgag gaagagaag aagcgactt gaaagagag 
      tgcgctctt gataacatc aagcaacac aaactgaaa 
      ttaaagaag aaagccgaa gatgtggtg aagactttg 
      gatgaagag cgttacttg attatgaaa ttaaaagaa 
      ttggaaatc gccgagaga cttgaagat gaaatgaag 
      aagattgaa gttaaagcc gcactagac cagtttttg 
      gccttgaat gaagaacag aagaaactt gagaagatt 
      cgccaaaag aatttggat tttttgttc gattcagag 
      gccaagaca atgtacgag caacaggag aaattctgg 
      aaggaagag gaagcttcc agggcgaag ttattgaaa 
      gatgttatt gatacgatt caaggacag attcaaagt 
      aacattgag aagaatagg agaagacag ttggaggtg 
      attcaagaa cgggaagaa atgctcaag aagattgag 
      gagcacaac caggaaatg gcgcgtttg aaggaagaa 
      gaggaaacg aagaaacgg aaaatgatg tcgatgctt 
      aaaaatgat ctcgaaatg aagaatttg aagaagaag 
      gcgaaggaa aatgccgaa ttgagacga atagatgaa 
      gaattggaa cgagttcgc aaggaggaa gaacgtctc 
      aaacaagaa atactaaac atacaacgg aggcaagga 
      cctataaag tccacaaga agtaagtct tttttctgc 
      taa
    mRNA sequence
    • >TC014996 RA
      atcaaaaaa ataacaaga caaaaaatt tgtttgtgt 
      ttggtggac tttttctat tgaagatga atttgttca 
      agttatggc acgaaggcc accccctag ccaccgaat 
      ccgcgtgga aaatgagat gatcagacg gcgggaaaa 
      agagttttg tcacgaccg tctatggaa cggtcaaag 
      acgttacta cgaagattt tgaaaggaa gaatacaaa 
      atatgatga atggacgtc accccgata ttacgaaaa 
      tcagcaaaa acttatgga aaaaacgaa gaaagaacg 
      cgaacatga ggaaaatct tgagcggag gagagcaaa 
      attgaagaa attgcttta tgacgaaga aaagttaca 
      agaagctga attgcagat tcgcaaggt cagaaatat 
      gacctctgt taggtcgcc acgacctaa cgagatacc 
      aactgaaat tttgaagga gctcaataa tggtttgaa 
      attggagga agaagaaag aagaagaca ccaagcgga 
      actcaagtt gtatcacca atggaggag tagtaaccc 
      cttcttaaa tcattacga aaggatgca agccagtcg 
      ggatttaaa attgtcttg gttggacca acaaatcga 
      aaatcggat gaagaagga gaaggaaga ggaagaatg 
      tcgcaagtt gctaaaaga acgcgagaa aatcatgaa 
      agaagaaca ggagaagtt tgatcaaga acagaaaag 
      gttacaatt gaagaagga agaattaaa agcgagtct 
      ggaacaaca aattgacga gatgcgtct aaagacgca 
      aattagtga agatctgaa acgaaaaga agatgaaga 
      aaggaggaa gaaagccga attggatga aatcgcact 
      gaagcggat tgaggaaga gaagaagcg acttgaaag 
      agagtgcgc tcttgataa catcaagca acacaaact 
      gaaattaaa gaagaaagc cgaagatgt ggtgaagac 
      tttggatga agagcgtta cttgattat gaaattaaa 
      agaattgga aatcgccga gagacttga agatgaaat 
      gaagaagat tgaagttaa agccgcact agaccagtt 
      tttggcctt gaatgaaga acagaagaa acttgagaa 
      gattcgcca aaagaattt ggatttttt gttcgattc 
      agaggccaa gacaatgta cgagcaaca ggagaaatt 
      ctggaagga agaggaagc ttccagggc gaagttatt 
      gaaagatgt tattgatac gattcaagg acagattca 
      aagtaacat tgagaagaa taggagaag acagttgga 
      ggtgattca agaacggga agaaatgct caagaagat 
      tgaggagca caaccagga aatggcgcg tttgaagga 
      agaagagga aacgaagaa acggaaaat gatgtcgat 
      gcttaaaaa tgatctcga aatgaagaa tttgaagaa 
      gaaggcgaa ggaaaatgc cgaattgag acgaataga 
      tgaagaatt ggaacgagt tcgcaagga ggaagaacg 
      tctcaaaca agaaatact aaacataca acggaggca 
      aggacctat aaagtccac aagaagtaa gtctttttt 
      ctgctaaac taattacaa ataataaca aggttgctg 
      aactgtgaa aaaccgttt tacatttgc cgaaaaaat 
      gctcggaat gttggtatt tcctccgtc attgttttt 
      aataattgt aggtttcaa tatttggtt gcataattt 
      ttgtaatat tagttggtt ctattttga gccaaggtt 
      ctttctttt gtttttttt tttttgttt ttatggttg 
      aaatgctaa aatgtgatt ttactttaa tacaaatac 
      tcgacgaaa ccatattgt ggttttatt tcacacatt 
      tttcaacac ttttcttaa aatcccaaa ttttgacgt 
      gacgaagaa cagaagaaa ctggtgacg aattgttag 
      aagaaaagg aagattttt ttgaagatt aaaataata 
      tctacaaga ttaagaagt aattcaaga agaggaaaa 
      aaatttttt tgtctttga gttggttct attttcact 
      caatgtttt tttttgttt tttattctt attgctgaa 
      aggatttat cgcaaattt ttgctttgc ttgaattca 
      aaaataaat ttaaccatt ttgtctctt taattcttt 
      gactatctt ttactattt tcttaaaaa tccaaattt 
      taacttgac gaagaccag aagaaactg gtgacgaat 
      tcctaaaag aaaatgaag agttttttg gaagattga 
      aaataatat cgccaagat taagaagta attcaagaa 
      aaggaataa cattttttg gtatttgtt tagttggtt 
      tttgatttg tctttgact tgacaatgt tttttttat 
      tttttttat cctaatatt tttttattg ccgaaagaa 
      attatctta aatact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY