Status
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Gene information
General information
TC013186 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12614327..12618395
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013186
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10485

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber degenerated - Number of animals showing the phenotype: 4

    lateral oviduct necrotic - Number of animals showing the phenotype: 3

    vitellarium size decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013186 RA
      MAKLKKSDN GDGADQSTH SEVKMKKEL ALIDGVAII 
      VGVIIGSGI FVSPKGVLR NSGSVGQAL IVWILSGIL 
      SMIGALCYA ELGTMIPKS GGDYAYISE AFGPLPAFL 
      YLWVALFIL VPTGNAITA ITFAQYILQ PLWGACNPP 
      YEAVRLLAA AAVCLLTLI NCYNVKWVT RVQDIFTAT 
      KIFALCIIV AAGAYYLCI GHVEHFRDP MEGTNWQPG 
      YIALAFYSG LFSYSGWNY LNYVTEELK DPYKNLPRA 
      ICISMPVVT VIYVVTNLA YFVVLSRDD ILASDAVAV 
      TFGDKLLGA FSFFIPFFV ACSTFGSLN GAIFASSRL 
      FFVGARAGH LPRAIALID VKRLTPVPS LIFMCIITL 
      ALVMIEDVY VLINYVSFV EALFITISV TGLLYMRWK 
      RPDMHRPIK ISFILPVIF LLICGFLVI FPCYVSPLE 
      VGVGLGFIF CGIPVYLVT IAWKNKPDW LNKIFNEFN 
      NGCAKLFLC VTEYEDKES
    CDS sequence
    • >TC013186 RA
      atggcaaag ttaaagaaa agtgataac ggggacggg 
      gcggaccaa agcacacat tctgaggtc aaaatgaaa 
      aaggaattg gcgttaatt gacggcgtt gcgattatt 
      gttggggtg attatcggc tcggggatt tttgtctcc 
      cccaagggg gtgctccgg aactccggc tcggtgggg 
      caggctctc atcgtatgg atcctctca ggaatttta 
      tccatgatc ggggctcta tgttacgct gaattaggc 
      acaatgata ccgaaatcg gggggcgac tacgcctac 
      atcagcgag gctttcggg ccgttgccc gccttcctc 
      tacctctgg gtggccctt ttcatcctg gtcccgacc 
      ggaaacgcc atcacggcc ataaccttc gcccagtac 
      atcctccaa ccgctttgg ggcgcctgt aaccctccg 
      tacgaagcc gtgcgatta ctggcagcc gctgccgtt 
      tgtctacta actctgatt aattgctac aacgtcaag 
      tgggtgaca cgggtccaa gacatcttc accgccacc 
      aaaatcttc gccctgtgc attatcgtg gcagctgga 
      gcgtactac ttatgcatt ggtcacgtc gagcatttc 
      agggacccc atggaaggg accaactgg cagcccggg 
      tacattgcc ttggccttt tattccggc ctgttctcc 
      tactcgggc tggaattac ctcaactat gttaccgaa 
      gaactgaaa gatccctac aagaacctt cctcgcgca 
      atttgtata tcgatgcca gtggtcacc gtcatttac 
      gttgtgaca aaccttgcg tactttgtt gtcttgagc 
      agggatgat attctggcg tcagatgca gttgctgtt 
      acttttggt gataagtta ctgggcgcg ttttcgttt 
      ttcattccg tttttcgtg gcttgttcc acgtttggt 
      tcgttaaac ggtgctatt tttgcatcg tcacgcctt 
      ttcttcgtg ggggctcgt gctggacac ttgccacga 
      gctatcgca ctcattgat gtgaaaaga ctgacccct 
      gtaccatct ctaattttt atgtgcatc attacgcta 
      gcccttgta atgattgaa gatgtgtac gttcttatt 
      aactacgtt tcgttcgtt gaggccctt tttatcacg 
      ataagtgtg acggggctg ctgtatatg aggtggaag 
      aggcccgat atgcaccgg ccgattaaa atcagtttt 
      attcttcct gtaatattt ttgttaatt tgtggcttc 
      cttgtcatt tttccctgt tatgttagt cccctggag 
      gtaggggta ggtttaggt ttcattttt tgcggaatt 
      cctgtttat ttagttacg atcgcttgg aagaataaa 
      ccagattgg ttgaataaa attttcaac gagtttaac 
      aacggctgc gccaaactc ttcctgtgt gttacggaa 
      tacgaagac aaggagtcc taa
    mRNA sequence
    • >TC013186 RA
      gcacacgct ccattcagc ttgcattac ctcaagttc 
      tcttaaccc taatggaaa tgtaaatat catacaaag 
      tcgcttaca aggaggatg tacgacacg taccgcgag 
      ttcgagacg gcgcacaac gaaagtgcg gaacggtgg 
      ccaatgttt tagttttaa aatacaggt taaaaacac 
      gtggtgcgt gcagcggaa tgcttgttt cgagttttt 
      aaaaaaatt acaagttct tgcaaaatg gcaaagtta 
      aagaaaagt gataacggg gacggggcg gaccaaagc 
      acacattct gaggtcaaa atgaaaaag gaattggcg 
      ttaattgac ggcgttgcg attattgtt ggggtgatt 
      atcggctcg gggattttt gtctccccc aagggggtg 
      ctccggaac tccggctcg gtggggcag gctctcatc 
      gtatggatc ctctcagga attttatcc atgatcggg 
      gctctatgt tacgctgaa ttaggcaca atgataccg 
      aaatcgggg ggcgactac gcctacatc agcgaggct 
      ttcgggccg ttgcccgcc ttcctctac ctctgggtg 
      gcccttttc atcctggtc ccgaccgga aacgccatc 
      acggccata accttcgcc cagtacatc ctccaaccg 
      ctttggggc gcctgtaac cctccgtac gaagccgtg 
      cgattactg gcagccgct gccgtttgt ctactaact 
      ctgattaat tgctacaac gtcaagtgg gtgacacgg 
      gtccaagac atcttcacc gccaccaaa atcttcgcc 
      ctgtgcatt atcgtggca gctggagcg tactactta 
      tgcattggt cacgtcgag catttcagg gaccccatg 
      gaagggacc aactggcag cccgggtac attgccttg 
      gccttttat tccggcctg ttctcctac tcgggctgg 
      aattacctc aactatgtt accgaagaa ctgaaagat 
      ccctacaag aaccttcct cgcgcaatt tgtatatcg 
      atgccagtg gtcaccgtc atttacgtt gtgacaaac 
      cttgcgtac tttgttgtc ttgagcagg gatgatatt 
      ctggcgtca gatgcagtt gctgttact tttggtgat 
      aagttactg ggcgcgttt tcgtttttc attccgttt 
      ttcgtggct tgttccacg tttggttcg ttaaacggt 
      gctattttt gcatcgtca cgccttttc ttcgtgggg 
      gctcgtgct ggacacttg ccacgagct atcgcactc 
      attgatgtg aaaagactg acccctgta ccatctcta 
      atttttatg tgcatcatt acgctagcc cttgtaatg 
      attgaagat gtgtacgtt cttattaac tacgtttcg 
      ttcgttgag gcccttttt atcacgata agtgtgacg 
      gggctgctg tatatgagg tggaagagg cccgatatg 
      caccggccg attaaaatc agttttatt cttcctgta 
      atatttttg ttaatttgt ggcttcctt gtcattttt 
      ccctgttat gttagtccc ctggaggta ggggtaggt 
      ttaggtttc attttttgc ggaattcct gtttattta 
      gttacgatc gcttggaag aataaacca gattggttg 
      aataaaatt ttcaacgag tttaacaac ggctgcgcc 
      aaactcttc ctgtgtgtt acggaatac gaagacaag 
      gagtcctaa agttagaca tatgatatg taaaaatga 
      tctaaatag tattaattg ttttcgaat gctttgaca 
      caagaaata tttatttgt atattacta ttcattaaa 
      catgttttg taaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY