Status
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Gene information
General information
TC002994 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5143040..5146035
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002994
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10484

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002994 RA
      MSLIKLFNL QSVGLLSKK VLNLSVHTR KFNDVPFED 
      IPEVSSTLI KRVLNKTKI SFNEGFTCF IIACPFCTK 
      RAKSNIHKL YINKTTGMF MCSSCKFTG QWNFLDHHF 
      NSKKEKSKK VQEICNVMD HQEKLAESL KQVRESSDL 
      LVNSDKFEK ILNKFKLPK ISSESIKNL DVRIDKPLQ 
      NLFFPLENV ECETVGYRK INYQNGDDL IIPHIMYGG 
      LIVGKALKN REAAILVPN ISDFLILLD SKITLNVVC 
      LPNGVNSLS QYVLPTLER FQKLILWFG NDLKSWDSA 
      RHFAKKLGE RRCFFVRPN EKQLLPHLV EGQDFKSIV 
      NSAQPIWHK SITNFANLR KDVFSDLQN IDKIEGVKW 
      KRFPALNKI LRGHRRGEL TVITGPTGC GKTTFISEY 
      SLDLAMQGV NTLWGSFEI RNVRLARTM LQQFAGFPL 
      DENLSQFDS LADKFELLP IYFMTFHGQ QTIKVVMDA 
      VEHATYVHD IAHVIIDNV QFMMGITED SRHMDRFWT 
      QDVIIAAFR SFATRKNCH VTLVIHPRK ERDEENLTT 
      NSIFGGAKA SQEADNVFI IQDKRLTST RGKKYLQIC 
      KNRYSGDLG VMPLDFDKA GLSYASKKK VKSTDNIEE 
      TTN
    CDS sequence
    • >TC002994 RA
      atgtctctt atcaagtta tttaattta caaagtgtt 
      ggtcttttg agcaaaaaa gtgttgaac ttaagtgtg 
      cataccagg aaattcaat gacgttccg tttgaagac 
      attccagaa gtgtcctca accttgata aaaagggtt 
      ctcaataaa acaaaaata agtttcaat gagggtttt 
      acgtgcttt ataattgcg tgtccgttt tgtacgaaa 
      cgagcaaaa agcaatatt cacaagtta tacattaat 
      aaaaccaca ggaatgttt atgtgttca tcgtgtaag 
      ttcactgga cagtggaat ttcctagac catcatttt 
      aattcaaaa aaggagaaa agtaaaaag gttcaagaa 
      atttgtaac gtgatggat catcaagag aaattagca 
      gaatcttta aaacaagtc agggaatcg agtgattta 
      ctggttaat agcgataag tttgaaaag atccttaat 
      aagtttaag ctcccaaaa atttcaagt gaatctatt 
      aaaaaccta gatgtaagg atagataag ccactacag 
      aatctcttt tttccgtta gaaaacgtt gagtgtgaa 
      acagtgggg tacaggaaa attaattac caaaacggg 
      gatgatctg ataattcct cacataatg tatgggggt 
      ttaattgtg gggaaagca ttaaaaaat agggaagct 
      gccatatta gtaccaaat atatccgat tttttaatt 
      cttttggat tccaaaata actctaaac gtcgtttgt 
      ttgccaaat ggcgtaaac agtttatct cagtatgtt 
      ttaccaact ttggaaaga ttccaaaaa ctcattctt 
      tggtttggt aacgattta aaatcatgg gactctgca 
      aggcatttt gcaaaaaaa ttgggcgaa aggaggtgc 
      ttttttgtc agaccaaac gaaaaacaa ctattgcca 
      caccttgtt gaaggccag gacttcaaa tcaattgtt 
      aattcagct cagcctata tggcacaag tctataaca 
      aactttgcc aatttgagg aaagatgtt ttctctgat 
      ttgcaaaac attgataag attgaggga gtaaagtgg 
      aagcggttt cccgctttg aataaaata ctgcgggga 
      caccgcagg ggcgaactg actgtgata accggacct 
      acaggttgt ggtaaaacg actttcata agcgaatat 
      tccctagat ttggcaatg caaggtgtg aatacgttg 
      tggggcagc tttgagata cgcaatgtt cgtttagct 
      aggacgatg ttgcagcag tttgctggg tttcctctt 
      gatgaaaat ttgtcccaa tttgactct ttggccgac 
      aaatttgag cttttgccc atatatttt atgaccttt 
      catggccaa caaacgata aaagttgtg atggacgcg 
      gtggaacat gctacctat gtgcatgat atcgcccat 
      gttattatt gataatgtc cagttcatg atgggaatc 
      actgaagat tcaagacat atggaccgg ttttggacg 
      caggatgtt ataattgcg gcttttcgc agcttcgct 
      accagaaaa aattgccac gttacgttg gtaatccat 
      cccaggaaa gaaagggat gaggagaat ctcaccaca 
      aattccatt tttggaggc gccaaagcg tcacaagaa 
      gccgataat gtgtttatc atccaagat aaaagactg 
      acgtccaca agaggcaaa aaatactta caaatttgt 
      aaaaacaga tacagtggt gatttaggt gtgatgcct 
      ttggatttc gataaggca ggtttaagc tacgcatca 
      aagaaaaaa gtcaaaagt actgacaat atcgaagaa 
      actacaaat tga
    mRNA sequence
    • >TC002994 RA
      cctggcttt ttgacattt gacagaaat ggcgcacat 
      gttttctgt tttgtatac aaatgataa ttttttagt 
      atttgattt aaaaaatgt ctcttatca agttattta 
      atttacaaa gtgttggtc ttttgagca aaaaagtgt 
      tgaacttaa gtgtgcata ccaggaaat tcaatgacg 
      ttccgtttg aagacattc cagaagtgt cctcaacct 
      tgataaaaa gggttctca ataaaacaa aaataagtt 
      tcaatgagg gttttacgt gctttataa ttgcgtgtc 
      cgttttgta cgaaacgag caaaaagca atattcaca 
      agttataca ttaataaaa ccacaggaa tgtttatgt 
      gttcatcgt gtaagttca ctggacagt ggaatttcc 
      tagaccatc attttaatt caaaaaagg agaaaagta 
      aaaaggttc aagaaattt gtaacgtga tggatcatc 
      aagagaaat tagcagaat ctttaaaac aagtcaggg 
      aatcgagtg atttactgg ttaatagcg ataagtttg 
      aaaagatcc ttaataagt ttaagctcc caaaaattt 
      caagtgaat ctattaaaa acctagatg taaggatag 
      ataagccac tacagaatc tcttttttc cgttagaaa 
      acgttgagt gtgaaacag tggggtaca ggaaaatta 
      attaccaaa acggggatg atctgataa ttcctcaca 
      taatgtatg ggggtttaa ttgtgggga aagcattaa 
      aaaataggg aagctgcca tattagtac caaatatat 
      ccgattttt taattcttt tggattcca aaataactc 
      taaacgtcg tttgtttgc caaatggcg taaacagtt 
      tatctcagt atgttttac caactttgg aaagattcc 
      aaaaactca ttctttggt ttggtaacg atttaaaat 
      catgggact ctgcaaggc attttgcaa aaaaattgg 
      gcgaaagga ggtgctttt ttgtcagac caaacgaaa 
      aacaactat tgccacacc ttgttgaag gccaggact 
      tcaaatcaa ttgttaatt cagctcagc ctatatggc 
      acaagtcta taacaaact ttgccaatt tgaggaaag 
      atgttttct ctgatttgc aaaacattg ataagattg 
      agggagtaa agtggaagc ggtttcccg ctttgaata 
      aaatactgc ggggacacc gcaggggcg aactgactg 
      tgataaccg gacctacag gttgtggta aaacgactt 
      tcataagcg aatattccc tagatttgg caatgcaag 
      gtgtgaata cgttgtggg gcagctttg agatacgca 
      atgttcgtt tagctagga cgatgttgc agcagtttg 
      ctgggtttc ctcttgatg aaaatttgt cccaatttg 
      actctttgg ccgacaaat ttgagcttt tgcccatat 
      attttatga cctttcatg gccaacaaa cgataaaag 
      ttgtgatgg acgcggtgg aacatgcta cctatgtgc 
      atgatatcg cccatgtta ttattgata atgtccagt 
      tcatgatgg gaatcactg aagattcaa gacatatgg 
      accggtttt ggacgcagg atgttataa ttgcggctt 
      ttcgcagct tcgctacca gaaaaaatt gccacgtta 
      cgttggtaa tccatccca ggaaagaaa gggatgagg 
      agaatctca ccacaaatt ccatttttg gaggcgcca 
      aagcgtcac aagaagccg ataatgtgt ttatcatcc 
      aagataaaa gactgacgt ccacaagag gcaaaaaat 
      acttacaaa tttgtaaaa acagataca gtggtgatt 
      taggtgtga tgcctttgg atttcgata aggcaggtt 
      taagctacg catcaaaga aaaaagtca aaagtactg 
      acaatatcg aagaaacta caaattgat ttttgtaat 
      aaatcattt cgtaaaga
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