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Gene information
General information
TC009147 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3810151..3812275
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009147
    iBeetle screen iB_04598

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Fertility
    egg chamber mostlynot present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva segment central not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    iBeetle screen iB_10481

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009147 RA
      MALTVEQCD ILLDILGET KVRTHTLES LSNELHKKF 
DSCDYFKVG TCLVMMLQQ DLLKEPEQK LVAITLLYE 
LYRGEALAN TPFANVFNH LLHPPEHQS SVGSPKQEY 
PGQLPKLTP PEKQFLTQL LSDVPKDAI LKKTATQII 
NSDVGNTQT NFDFSVVQL LLAEKQSEL PHTCKTGIP 
VVLSLPEKT NNYTTERND YAALVKTIA TGDNAPINK 
VYKPEFVTL APPLLNTDD EMVWLNPTN IKEHTVAYD 
VTMCVPDTA GQEARQLMN KAYKSALSL QQQQQLLGE 
LENDSKLVY HIGLTPSKL PELVENNPL IAIEVLLKL 
MQSKQITEY FSVLVNMEM SLHSMEVVN RLTTTVDLP 
TEFVHLYIS NCISTCETI KDRYMQNRL VRLVCVFLQ 
SLIRNKIIN VQELFIEVQ AFCIEFSRI REAAALFRL 
LKQLESGEQ IGLVSSPTI MKAKLEG
    CDS sequence
    • >TC009147 RA
      atggccctc acggtggaa caatgtgat atcttactc 
gatattttg ggtgagaca aaggtgaga acccacact 
ttagaaagc ctttcgaac gagttgcac aaaaaattc 
gactcttgt gactatttt aaagtcgga acttgtctg 
gtaatgatg cttcagcaa gatctgttg aaagagcct 
gagcaaaaa cttgttgct ataacgctt ctgtatgag 
ttatatcgg ggcgaagct ctggcaaac acccctttt 
gccaacgtt ttcaaccac cttttgcac cccccggaa 
caccagagt agtgtcggg tcccccaaa caggaatat 
ccaggccaa ctacccaaa ctgacacca ccagaaaaa 
caattctta acgcaactg ttatcagat gtgcccaaa 
gatgctatt ttgaaaaaa acagccacg caaataatc 
aattcagac gttgggaat acgcaaaca aatttcgat 
ttttccgtt gtacaatta ctactagcc gaaaaacag 
tctgaattg ccacacact tgcaaaaca ggaattcct 
gtagtttta tcactgcct gaaaaaact aacaattat 
acgacagaa cggaacgat tatgcagcc cttgtgaaa 
actatagca accggtgat aacgccccc attaataaa 
gtgtacaaa ccggaattt gtgacacta gcaccgcct 
ttgctcaac acagatgat gaaatggtg tggctaaat 
ccgacaaac atcaaagaa cacactgta gcatatgac 
gtgacgatg tgtgtgcct gacacagca ggccaggag 
gcgagacaa ttgatgaac aaggcgtac aaatcagcg 
ctgtctttg caacagcag caacaactg ctaggcgag 
ctggaaaac gactcaaaa cttgtgtac cacattggt 
ttgactccg tccaaattg cccgaactg gtggaaaat 
aatccactt atcgcgatt gaagttttg ctcaagctt 
atgcaatcg aaacaaata acggaatat ttcagcgtt 
cttgtcaac atggaaatg tcgctgcat tccatggaa 
gtggtaaat cggctcaca acgaccgtg gatttgccc 
acagagttt gtccacttg tacatttcg aattgcata 
tcgacttgt gagactatc aaggaccgt tatatgcaa 
aatcggctg gttcgactg gtttgtgtg tttctccag 
tcgctcatt cgcaacaaa ataatcaac gtccaggag 
ttgtttatc gaagttcag gcgttttgt atcgagttt 
agtcgcatt cgcgaagct gccgctttg ttcaggcta 
ttgaaacag ttggagtcg ggggaacag atcggactt 
gtctcctcg ccaacgata atgaaagca aagctcgag 
ggttga
    mRNA sequence
    • >TC009147 RA
      tagagtata taaaataaa tttcgtaaa aataggtgt 
tttattgca aaacgatgg ccctcacgg tggaacaat 
gtgatatct tactcgata ttttgggtg agacaaagg 
tgagaaccc acactttag aaagccttt cgaacgagt 
tgcacaaaa aattcgact cttgtgact attttaaag 
tcggaactt gtctggtaa tgatgcttc agcaagatc 
tgttgaaag agcctgagc aaaaacttg ttgctataa 
cgcttctgt atgagttat atcggggcg aagctctgg 
caaacaccc cttttgcca acgttttca accaccttt 
tgcaccccc cggaacacc agagtagtg tcgggtccc 
ccaaacagg aatatccag gccaactac ccaaactga 
caccaccag aaaaacaat tcttaacgc aactgttat 
cagatgtgc ccaaagatg ctattttga aaaaaacag 
ccacgcaaa taatcaatt cagacgttg ggaatacgc 
aaacaaatt tcgattttt ccgttgtac aattactac 
tagccgaaa aacagtctg aattgccac acacttgca 
aaacaggaa ttcctgtag ttttatcac tgcctgaaa 
aaactaaca attatacga cagaacgga acgattatg 
cagcccttg tgaaaacta tagcaaccg gtgataacg 
cccccatta ataaagtgt acaaaccgg aatttgtga 
cactagcac cgcctttgc tcaacacag atgatgaaa 
tggtgtggc taaatccga caaacatca aagaacaca 
ctgtagcat atgacgtga cgatgtgtg tgcctgaca 
cagcaggcc aggaggcga gacaattga tgaacaagg 
cgtacaaat cagcgctgt ctttgcaac agcagcaac 
aactgctag gcgagctgg aaaacgact caaaacttg 
tgtaccaca ttggtttga ctccgtcca aattgcccg 
aactggtgg aaaataatc cacttatcg cgattgaag 
ttttgctca agcttatgc aatcgaaac aaataacgg 
aatatttca gcgttcttg tcaacatgg aaatgtcgc 
tgcattcca tggaagtgg taaatcggc tcacaacga 
ccgtggatt tgcccacag agtttgtcc acttgtaca 
tttcgaatt gcatatcga cttgtgaga ctatcaagg 
accgttata tgcaaaatc ggctggttc gactggttt 
gtgtgtttc tccagtcgc tcattcgca acaaaataa 
tcaacgtcc aggagttgt ttatcgaag ttcaggcgt 
tttgtatcg agtttagtc gcattcgcg aagctgccg 
ctttgttca ggctattga aacagttgg agtcggggg 
aacagatcg gacttgtct cctcgccaa cgataatga 
aagcaaagc tcgagggtt gattattta atggaactg 
tatcgtaca atgtggcag gccgtgctg taatttatc 
gacgatttt ttccctctc tttgacgta cggcagatc 
ggccagaat cagctcttt taagttatt caacgcgtt 
gaggtgact cagccccgg ctctgttat attcaaaca 
ctcggtgat ttgtaaata aagtaatga gttttttaa 
aaccgacag ccctttcaa cgcctcatt ctccagata 
gtagcactt gtggaaaat gattttccg gatgtgttc 
gcaccccac gaaatggtc aaacccgta gtgcattat 
gctggcttc agacgcgtt cacttccac gatgtgtaa 
cctaatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY