General information | |||
TC009147 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3810151..3812275 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC009147 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04598 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva segment central not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
ovary without detectable defects - Number of animals showing the phenotype: 4
fertility after larval injection decreased (no living offspring) -
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_10481 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC009147 RA
MALTVEQCD ILLDILGET KVRTHTLES LSNELHKKF DSCDYFKVG TCLVMMLQQ DLLKEPEQK LVAITLLYE LYRGEALAN TPFANVFNH LLHPPEHQS SVGSPKQEY PGQLPKLTP PEKQFLTQL LSDVPKDAI LKKTATQII NSDVGNTQT NFDFSVVQL LLAEKQSEL PHTCKTGIP VVLSLPEKT NNYTTERND YAALVKTIA TGDNAPINK VYKPEFVTL APPLLNTDD EMVWLNPTN IKEHTVAYD VTMCVPDTA GQEARQLMN KAYKSALSL QQQQQLLGE LENDSKLVY HIGLTPSKL PELVENNPL IAIEVLLKL MQSKQITEY FSVLVNMEM SLHSMEVVN RLTTTVDLP TEFVHLYIS NCISTCETI KDRYMQNRL VRLVCVFLQ SLIRNKIIN VQELFIEVQ AFCIEFSRI REAAALFRL LKQLESGEQ IGLVSSPTI MKAKLEG
- >TC009147 RA
atggccctc acggtggaa caatgtgat atcttactc gatattttg ggtgagaca aaggtgaga acccacact ttagaaagc ctttcgaac gagttgcac aaaaaattc gactcttgt gactatttt aaagtcgga acttgtctg gtaatgatg cttcagcaa gatctgttg aaagagcct gagcaaaaa cttgttgct ataacgctt ctgtatgag ttatatcgg ggcgaagct ctggcaaac acccctttt gccaacgtt ttcaaccac cttttgcac cccccggaa caccagagt agtgtcggg tcccccaaa caggaatat ccaggccaa ctacccaaa ctgacacca ccagaaaaa caattctta acgcaactg ttatcagat gtgcccaaa gatgctatt ttgaaaaaa acagccacg caaataatc aattcagac gttgggaat acgcaaaca aatttcgat ttttccgtt gtacaatta ctactagcc gaaaaacag tctgaattg ccacacact tgcaaaaca ggaattcct gtagtttta tcactgcct gaaaaaact aacaattat acgacagaa cggaacgat tatgcagcc cttgtgaaa actatagca accggtgat aacgccccc attaataaa gtgtacaaa ccggaattt gtgacacta gcaccgcct ttgctcaac acagatgat gaaatggtg tggctaaat ccgacaaac atcaaagaa cacactgta gcatatgac gtgacgatg tgtgtgcct gacacagca ggccaggag gcgagacaa ttgatgaac aaggcgtac aaatcagcg ctgtctttg caacagcag caacaactg ctaggcgag ctggaaaac gactcaaaa cttgtgtac cacattggt ttgactccg tccaaattg cccgaactg gtggaaaat aatccactt atcgcgatt gaagttttg ctcaagctt atgcaatcg aaacaaata acggaatat ttcagcgtt cttgtcaac atggaaatg tcgctgcat tccatggaa gtggtaaat cggctcaca acgaccgtg gatttgccc acagagttt gtccacttg tacatttcg aattgcata tcgacttgt gagactatc aaggaccgt tatatgcaa aatcggctg gttcgactg gtttgtgtg tttctccag tcgctcatt cgcaacaaa ataatcaac gtccaggag ttgtttatc gaagttcag gcgttttgt atcgagttt agtcgcatt cgcgaagct gccgctttg ttcaggcta ttgaaacag ttggagtcg ggggaacag atcggactt gtctcctcg ccaacgata atgaaagca aagctcgag ggttga
- >TC009147 RA
tagagtata taaaataaa tttcgtaaa aataggtgt tttattgca aaacgatgg ccctcacgg tggaacaat gtgatatct tactcgata ttttgggtg agacaaagg tgagaaccc acactttag aaagccttt cgaacgagt tgcacaaaa aattcgact cttgtgact attttaaag tcggaactt gtctggtaa tgatgcttc agcaagatc tgttgaaag agcctgagc aaaaacttg ttgctataa cgcttctgt atgagttat atcggggcg aagctctgg caaacaccc cttttgcca acgttttca accaccttt tgcaccccc cggaacacc agagtagtg tcgggtccc ccaaacagg aatatccag gccaactac ccaaactga caccaccag aaaaacaat tcttaacgc aactgttat cagatgtgc ccaaagatg ctattttga aaaaaacag ccacgcaaa taatcaatt cagacgttg ggaatacgc aaacaaatt tcgattttt ccgttgtac aattactac tagccgaaa aacagtctg aattgccac acacttgca aaacaggaa ttcctgtag ttttatcac tgcctgaaa aaactaaca attatacga cagaacgga acgattatg cagcccttg tgaaaacta tagcaaccg gtgataacg cccccatta ataaagtgt acaaaccgg aatttgtga cactagcac cgcctttgc tcaacacag atgatgaaa tggtgtggc taaatccga caaacatca aagaacaca ctgtagcat atgacgtga cgatgtgtg tgcctgaca cagcaggcc aggaggcga gacaattga tgaacaagg cgtacaaat cagcgctgt ctttgcaac agcagcaac aactgctag gcgagctgg aaaacgact caaaacttg tgtaccaca ttggtttga ctccgtcca aattgcccg aactggtgg aaaataatc cacttatcg cgattgaag ttttgctca agcttatgc aatcgaaac aaataacgg aatatttca gcgttcttg tcaacatgg aaatgtcgc tgcattcca tggaagtgg taaatcggc tcacaacga ccgtggatt tgcccacag agtttgtcc acttgtaca tttcgaatt gcatatcga cttgtgaga ctatcaagg accgttata tgcaaaatc ggctggttc gactggttt gtgtgtttc tccagtcgc tcattcgca acaaaataa tcaacgtcc aggagttgt ttatcgaag ttcaggcgt tttgtatcg agtttagtc gcattcgcg aagctgccg ctttgttca ggctattga aacagttgg agtcggggg aacagatcg gacttgtct cctcgccaa cgataatga aagcaaagc tcgagggtt gattattta atggaactg tatcgtaca atgtggcag gccgtgctg taatttatc gacgatttt ttccctctc tttgacgta cggcagatc ggccagaat cagctcttt taagttatt caacgcgtt gaggtgact cagccccgg ctctgttat attcaaaca ctcggtgat ttgtaaata aagtaatga gttttttaa aaccgacag ccctttcaa cgcctcatt ctccagata gtagcactt gtggaaaat gattttccg gatgtgttc gcaccccac gaaatggtc aaacccgta gtgcattat gctggcttc agacgcgtt cacttccac gatgtgtaa cctaatc
