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Gene information
General information
TC004808 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2097931..2099625
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004808
      iBeetle screen iB_10476

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004808 RA
        MEHIPSLLR SVCPDSAIV KDIKCSRTK ATAITNECM 
        ALESLECLQ KHLQESGNT FSLIIDETT DVSVKKSLA 
        IVIRYCFNN LPRDRVFGI VKVEESNAE SLFNIIIKL 
        LKDNKIDLK DMLGFAADN ASVMMGHIS GIKARFHEL 
        NPNIFVLGC TCHSLHLAA SAACSKIPK DLEEFVRNL 
        YNYFSNSPK RINQLEEFQ KFLDLKPFK MIRPCQTRW 
        LSLQAVMDR IILLWDSLQ LHFTNFLFE SLRNALDCE 
        TKLLEGLRN PVYKMYLLF LSYILGIVN QIILQFQSE 
        GTQIHLLYN KFTALYNTV LRNYIKKSV IDKTELPKI 
        ALANPDYFV ELDNLYMGA NVDLFIQQI NIDATEDQK 
        FRTNILSFY IELANQIRK RFNFEDKFL MFLSTFEPK 
        VAMSGEIPS IVKSNLYFG NNIITNLEQ LNNEWRLLP 
        DIACLKRYT NFNFIEFWI KVFNLKNEI NMPMFPNLT 
        KLVKAIMCL PHSSAAADR IFSKLNLIK SDSRNRLSV 
        ETCNSIILS TELIEGTKK CFNWEPQKS LLEKNGTYG 
        KQRH
      CDS sequence
      • >TC004808 RA
        atggaacat ataccaagt cttctgcga tcagtgtgt 
        cctgattct gctattgta aaagacatt aaatgcagt 
        agaacaaaa gccactgct ataactaat gaatgcatg 
        gcgttagaa agtctggaa tgcttacaa aagcacctt 
        caagaatct ggaaatact ttctccctc atcattgat 
        gaaacgact gatgtgtct gtgaagaag tctttggct 
        attgtaata agatattgc tttaataat ttgccaaga 
        gatagggtt tttggaata gtgaaagtt gaagaatca 
        aatgcagaa tctctgttt aatattatt attaaatta 
        ttgaaagat aataagatc gatttaaaa gacatgctc 
        ggatttgca gcagataat gctagcgta atgatgggc 
        catatttcc ggcattaaa gctagattt catgaactc 
        aacccaaac atctttgta ctgggttgc acgtgtcat 
        tcattgcat ctagcagca tctgctgct tgttcaaaa 
        ataccaaaa gacctagaa gaatttgta agaaattta 
        tataactat ttttctaat agtcctaaa cgtattaat 
        cagttagaa gaatttcaa aaatttttg gatctaaaa 
        ccgtttaaa atgattcga ccatgccaa actagatgg 
        ctgtcctta caggcagta atggacaga ataatttta 
        ttgtgggat tctctgcaa ctacatttt acgaatttt 
        ttgtttgaa agtttgaga aatgcttta gactgcgaa 
        actaaattg ttagaaggt ttaagaaac cctgtatat 
        aagatgtat cttctcttt ttgtcttac attttagga 
        attgttaac caaataatt ttgcagttt caaagtgaa 
        ggtactcaa attcatctt ttgtacaac aaatttact 
        gctctttac aatactgtt ttaagaaat tatattaaa 
        aagtctgtt attgataag actgagtta ccaaaaatt 
        gcacttgct aatcctgat tattttgtg gagctagat 
        aatctttat atgggtgca aatgtagat ctatttata 
        caacaaatt aatattgac gcgacagaa gatcaaaag 
        tttagaact aatatatta tctttttat attgaactc 
        gctaatcaa ataaggaaa cgttttaat tttgaagac 
        aagtttcta atgtttctt tcaactttc gaacctaaa 
        gttgcaatg agtggcgaa attccttct atagtgaaa 
        agtaattta tattttggc aacaacatc ataactaat 
        ttagaacaa ttaaacaac gaatggaga cttttacca 
        gacattgcc tgtttaaaa cggtataca aattttaat 
        tttattgaa ttttggata aaagttttt aacttgaaa 
        aatgaaatt aatatgcca atgtttcct aacttgact 
        aaacttgtg aaagccata atgtgtcta cctcattca 
        tcagccgcc gccgatcgt atcttttct aaattaaat 
        cttattaag agtgattcc agaaatcgt ttaagtgtt 
        gaaacatgc aactcaatt atattaagt actgaactg 
        atagaaggt acaaagaaa tgttttaac tgggaacca 
        caaaaatca ttgttagag aaaaacgga acatatgga 
        aaacaaaga cattaa
      mRNA sequence
      • >TC004808 RA
        atggaacat ataccaagt cttctgcga tcagtgtgt 
        cctgattct gctattgta aaagacatt aaatgcagt 
        agaacaaaa gccactgct ataactaat gaatgcatg 
        gcgttagaa agtctggaa tgcttacaa aagcacctt 
        caagaatct ggaaatact ttctccctc atcattgat 
        gaaacgact gatgtgtct gtgaagaag tctttggct 
        attgtaata agatattgc tttaataat ttgccaaga 
        gatagggtt tttggaata gtgaaagtt gaagaatca 
        aatgcagaa tctctgttt aatattatt attaaatta 
        ttgaaagat aataagatc gatttaaaa gacatgctc 
        ggatttgca gcagataat gctagcgta atgatgggc 
        catatttcc ggcattaaa gctagattt catgaactc 
        aacccaaac atctttgta ctgggttgc acgtgtcat 
        tcattgcat ctagcagca tctgctgct tgttcaaaa 
        ataccaaaa gacctagaa gaatttgta agaaattta 
        tataactat ttttctaat agtcctaaa cgtattaat 
        cagttagaa gaatttcaa aaatttttg gatctaaaa 
        ccgtttaaa atgattcga ccatgccaa actagatgg 
        ctgtcctta caggcagta atggacaga ataatttta 
        ttgtgggat tctctgcaa ctacatttt acgaatttt 
        ttgtttgaa agtttgaga aatgcttta gactgcgaa 
        actaaattg ttagaaggt ttaagaaac cctgtatat 
        aagatgtat cttctcttt ttgtcttac attttagga 
        attgttaac caaataatt ttgcagttt caaagtgaa 
        ggtactcaa attcatctt ttgtacaac aaatttact 
        gctctttac aatactgtt ttaagaaat tatattaaa 
        aagtctgtt attgataag actgagtta ccaaaaatt 
        gcacttgct aatcctgat tattttgtg gagctagat 
        aatctttat atgggtgca aatgtagat ctatttata 
        caacaaatt aatattgac gcgacagaa gatcaaaag 
        tttagaact aatatatta tctttttat attgaactc 
        gctaatcaa ataaggaaa cgttttaat tttgaagac 
        aagtttcta atgtttctt tcaactttc gaacctaaa 
        gttgcaatg agtggcgaa attccttct atagtgaaa 
        agtaattta tattttggc aacaacatc ataactaat 
        ttagaacaa ttaaacaac gaatggaga cttttacca 
        gacattgcc tgtttaaaa cggtataca aattttaat 
        tttattgaa ttttggata aaagttttt aacttgaaa 
        aatgaaatt aatatgcca atgtttcct aacttgact 
        aaacttgtg aaagccata atgtgtcta cctcattca 
        tcagccgcc gccgatcgt atcttttct aaattaaat 
        cttattaag agtgattcc agaaatcgt ttaagtgtt 
        gaaacatgc aactcaatt atattaagt actgaactg 
        atagaaggt acaaagaaa tgttttaac tgggaacca 
        caaaaatca ttgttagag aaaaacgga acatatgga 
        aaacaaaga cattaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY