Status
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Gene information
General information
TC015927 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4237718..4252302
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC015927
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_10474

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015927 RA
        MSPQQTKVN TANKSTPTP SGRNQEVAT LVVMIVVLT 
        IIILFCCFA APGVRDLCK KYVFRTCVM DDPDIDNAS 
        AAGESATPT IILLPYGRM LVVDRAVFA QLQADHTGI 
        DFMELSANL IRAQRYQAG STPSILDSE TTSKGLSPT 
        SLGFSPPAY EDIFGDKSD LPPSYSEVS LMFRTQRRL 
        QEPESIEMC EVEEGGLSD ADVNNENNF NKELDEESV 
        RIAVEECDA STSETGEDG EVKESRI
      • >TC015927 RB
        MSPQQTKVN TANKSTPTP SGRNQEVAT LVVMIVVLT 
        IIILFCCFA APGVRDLCK KYVFRTCVM DDPDIDNVY 
        TRRCRRRDE FKVISETNK NKGQVETSA AGESATPTI 
        ILLPYGRML VVDRAVFAQ LQADHTGID FMELSANLI 
        RAQRYQAGS TPSILDSET TSKGLSPTS LGFSPPAYE 
        DIFGDKSDL PPSYSEVSL MFRTQRRLQ EPESIEMCE 
        VEEGGLSDA DVNNENNFN KELDEESVR IAVEECDAS 
        TSETGEDGE VKESRI
      CDS sequence
      • >TC015927 RA
        atgagtccg caacagaca aaagtaaac acggccaac 
        aaatccact ccaacgcca agcggaaga aaccaggaa 
        gtggcgact ctggttgtt atgatagta gtgctgaca 
        attatcata ctgttctgc tgttttgct gcgcctggt 
        gtcagggat ttgtgcaag aagtacgtg ttccgcacc 
        tgtgtcatg gacgatcct gatatagac aatgctagc 
        gccgcagga gaatcagcg acgccaaca ataatcctg 
        ctgccgtac ggcaggatg ctggtggtg gaccgcgcc 
        gtgttcgcc cagctccag gccgaccac accggcatc 
        gattttatg gaactgagt gcgaacctg atccgagct 
        caacgctac caagccgga agcaccccc agcatcctg 
        gactcggaa acgaccagc aaggggctg tcgcccacc 
        tctctgggc ttctcgccg ccggcttac gaggacatc 
        ttcggggac aaatccgat cttccgccg tcgtacagt 
        gaagttagt ttaatgttt aggacgcaa aggagactg 
        caggagccg gaaagcatc gaaatgtgc gaagtggaa 
        gaggggggc ttgagtgat gcggatgtg aacaatgag 
        aacaatttt aataaggag ttggacgag gagagtgtg 
        aggattgcc gtggaggag tgcgatgcc tccacttcc 
        gagacgggg gaggatggc gaggttaag gagagtagg 
        atttaa
      • >TC015927 RB
        atgagtccg caacagaca aaagtaaac acggccaac 
        aaatccact ccaacgcca agcggaaga aaccaggaa 
        gtggcgact ctggttgtt atgatagta gtgctgaca 
        attatcata ctgttctgc tgttttgct gcgcctggt 
        gtcagggat ttgtgcaag aagtacgtg ttccgcacc 
        tgtgtcatg gacgatcct gatatagac aatgtatat 
        acgcgcaga tgtagacgc agggacgag tttaaagtt 
        atttcagaa acgaacaaa aacaaggga caggtcgaa 
        actagcgcc gcaggagaa tcagcgacg ccaacaata 
        atcctgctg ccgtacggc aggatgctg gtggtggac 
        cgcgccgtg ttcgcccag ctccaggcc gaccacacc 
        ggcatcgat tttatggaa ctgagtgcg aacctgatc 
        cgagctcaa cgctaccaa gccggaagc acccccagc 
        atcctggac tcggaaacg accagcaag gggctgtcg 
        cccacctct ctgggcttc tcgccgccg gcttacgag 
        gacatcttc ggggacaaa tccgatctt ccgccgtcg 
        tacagtgaa gttagttta atgtttagg acgcaaagg 
        agactgcag gagccggaa agcatcgaa atgtgcgaa 
        gtggaagag gggggcttg agtgatgcg gatgtgaac 
        aatgagaac aattttaat aaggagttg gacgaggag 
        agtgtgagg attgccgtg gaggagtgc gatgcctcc 
        acttccgag acgggggag gatggcgag gttaaggag 
        agtaggatt taa
      mRNA sequence
      • >TC015927 RA
        tcgctcgga aaacccgcc cgagttcgg ctctggact 
        tggccatca atgaaaaac tggacacga gtcgttaaa 
        atgcgagtt aggtgacaa ctttcttaa ggaaagtcc 
        cgacgtgat tggattttt gatgatgaa ggcagagga 
        agcaaagag tgcgcgatt ttactagcc agttggggg 
        caggatgca caagaacac gtgaaaaac gacaatgag 
        tccgcaaca gacaaaagt aaacacggc caacaaatc 
        cactccaac gccaagcgg aagaaacca ggaagtggc 
        gactctggt tgttatgat agtagtgct gacaattat 
        catactgtt ctgctgttt tgctgcgcc tggtgtcag 
        ggatttgtg caagaagta cgtgttccg cacctgtgt 
        catggacga tcctgatat agacaatgc tagcgccgc 
        aggagaatc agcgacgcc aacaataat cctgctgcc 
        gtacggcag gatgctggt ggtggaccg cgccgtgtt 
        cgcccagct ccaggccga ccacaccgg catcgattt 
        tatggaact gagtgcgaa cctgatccg agctcaacg 
        ctaccaagc cggaagcac ccccagcat cctggactc 
        ggaaacgac cagcaaggg gctgtcgcc cacctctct 
        gggcttctc gccgccggc ttacgagga catcttcgg 
        ggacaaatc cgatcttcc gccgtcgta cagtgaagt 
        tagtttaat gtttaggac gcaaaggag actgcagga 
        gccggaaag catcgaaat gtgcgaagt ggaagaggg 
        gggcttgag tgatgcgga tgtgaacaa tgagaacaa 
        ttttaataa ggagttgga cgaggagag tgtgaggat 
        tgccgtgga ggagtgcga tgcctccac ttccgagac 
        gggggagga tggcgaggt taaggagag taggattta 
        agacaccct tgttattgt tacactgtg ctgttattg 
        gggcagtcg ggcactaac gcattctaa atcaaacga 
        ttttgaacg aaaaattac actttacaa cgtttttaa 
        agataaaat tcggaactg ccaacaaaa tagtcactg 
        tctggattc ttaatttcg gagcagtga cgtggtttt 
        gaaattaag ttggtaaca cccaaaaaa aattatcga 
        acatctgta gaattacct acttttgtc cagcaaatt 
        tttttttaa ataaataga aaaatcacc aaattaaat 
        aagtaaaaa aatgtatcg tgtgttttt ttactaaat 
        acgtacatt tttcgttat ttttgcgtt tttcgtgtg 
        ttttagcac gattttagt gaaaatttt taattctcg 
        tgagtttga aagaccacc aaattatcg aaagtcatg 
        ttgttttac ttgattatt tgctcaatt tggcttatt 
        tgtgaacaa aattttgtc aaaaatgaa tccaaaatt 
        ttccaaaaa atgtgtaat tgttcattt ttatgagat 
        ttcattaaa tacgcttaa agtattgtg aaagtaaag 
        tgacaaatt gtgcttgaa agattacca gattctcaa 
        aaagggtgt gatgttttg aactagaaa tagatttac 
        ttcacaaac cttgccaaa caaaaaact aaaaaatgt 
        ttgtttgtg aacgaaaag ttgtcaaaa atatatcaa 
        aaaattatc catgtcgtt aaagaaatt gttatatgt 
        tctttttat gagatttta ctaaacacc gttgaaaaa 
        ctgccaaat accattgac aacaacttt atttttgat 
        ttttcgtga attttatta cgtttttag cgtccgaaa 
        ttttttttt attttgtta aaatagttt gaaaagacc 
        accaaatta tctaaactg atgcaatat ttgtttatt 
        tgaaccagt gactcatgt ctttacaaa gagttgcca 
        aagcacaca taaaaaata aaaagtaaa taaaaatcg 
        atgttgttt tgattgttt tactggatt ttgcttgtc 
        tgtgagtga aatttcgta aacaaaatt atcgaaaaa 
        tcaccaagg tagttatca caggcgaag tgttgttga 
        aatagatcc acaagggct ttatttttg tgttttttg 
        tgtattttt gtgcaaaaa caattttct ttaatttcg 
        ctaaaactg ttttaaaaa tcaccaaat tatcggaac 
        tgtaatttt ctcaatact ttgcaaaac aagcttaaa 
        aagcagaaa gtaaatcaa aactgatgt tatttccct 
        tctttcact catttttgc ctcgatttt tgctgtttg 
        tgtgcgaaa t
      • >TC015927 RB
        tcgctcgga aaacccgcc cgagttcgg ctctggact 
        tggccatca atgaaaaac tggacacga gtcgttaaa 
        atgcgagtt aggtgacaa ctttcttaa ggaaagtcc 
        cgacgtgat tggattttt gatgatgaa ggcagagga 
        agcaaagag tgcgcgatt ttactagcc agttggggg 
        caggatgca caagaacac gtgaaaaac gacaatgag 
        tccgcaaca gacaaaagt aaacacggc caacaaatc 
        cactccaac gccaagcgg aagaaacca ggaagtggc 
        gactctggt tgttatgat agtagtgct gacaattat 
        catactgtt ctgctgttt tgctgcgcc tggtgtcag 
        ggatttgtg caagaagta cgtgttccg cacctgtgt 
        catggacga tcctgatat agacaatgt atatacgcg 
        cagatgtag acgcaggga cgagtttaa agttatttc 
        agaaacgaa caaaaacaa gggacaggt cgaaactag 
        cgccgcagg agaatcagc gacgccaac aataatcct 
        gctgccgta cggcaggat gctggtggt ggaccgcgc 
        cgtgttcgc ccagctcca ggccgacca caccggcat 
        cgattttat ggaactgag tgcgaacct gatccgagc 
        tcaacgcta ccaagccgg aagcacccc cagcatcct 
        ggactcgga aacgaccag caaggggct gtcgcccac 
        ctctctggg cttctcgcc gccggctta cgaggacat 
        cttcgggga caaatccga tcttccgcc gtcgtacag 
        tgaagttag tttaatgtt taggacgca aaggagact 
        gcaggagcc ggaaagcat cgaaatgtg cgaagtgga 
        agagggggg cttgagtga tgcggatgt gaacaatga 
        gaacaattt taataagga gttggacga ggagagtgt 
        gaggattgc cgtggagga gtgcgatgc ctccacttc 
        cgagacggg ggaggatgg cgaggttaa ggagagtag 
        gatttaaga cacccttgt tattgttac actgtgctg 
        ttattgggg cagtcgggc actaacgca ttctaaatc 
        aaacgattt tgaacgaaa aattacact ttacaacgt 
        ttttaaaga taaaattcg gaactgcca acaaaatag 
        tcactgtct ggattctta atttcggag cagtgacgt 
        ggttttgaa attaagttg gtaacaccc aaaaaaaat 
        tatcgaaca tctgtagaa ttacctact tttgtccag 
        caaattttt ttttaaata aatagaaaa atcaccaaa 
        ttaaataag taaaaaaat gtatcgtgt gttttttta 
        ctaaatacg tacattttt cgttatttt tgcgttttt 
        cgtgtgttt tagcacgat tttagtgaa aatttttaa 
        ttctcgtga gtttgaaag accaccaaa ttatcgaaa 
        gtcatgttg ttttacttg attatttgc tcaatttgg 
        cttatttgt gaacaaaat tttgtcaaa aatgaatcc 
        aaaattttc caaaaaatg tgtaattgt tcattttta 
        tgagatttc attaaatac gcttaaagt attgtgaaa 
        gtaaagtga caaattgtg cttgaaaga ttaccagat 
        tctcaaaaa gggtgtgat gttttgaac tagaaatag 
        atttacttc acaaacctt gccaaacaa aaaactaaa 
        aaatgtttg tttgtgaac gaaaagttg tcaaaaata 
        tatcaaaaa attatccat gtcgttaaa gaaattgtt 
        atatgttct ttttatgag attttacta aacaccgtt 
        gaaaaactg ccaaatacc attgacaac aactttatt 
        tttgatttt tcgtgaatt ttattacgt ttttagcgt 
        ccgaaattt ttttttatt ttgttaaaa tagtttgaa 
        aagaccacc aaattatct aaactgatg caatatttg 
        tttatttga accagtgac tcatgtctt tacaaagag 
        ttgccaaag cacacataa aaaataaaa agtaaataa 
        aaatcgatg ttgttttga ttgttttac tggattttg 
        cttgtctgt gagtgaaat ttcgtaaac aaaattatc 
        gaaaaatca ccaaggtag ttatcacag gcgaagtgt 
        tgttgaaat agatccaca agggcttta tttttgtgt 
        tttttgtgt atttttgtg caaaaacaa ttttcttta 
        atttcgcta aaactgttt taaaaatca ccaaattat 
        cggaactgt aattttctc aatactttg caaaacaag 
        cttaaaaag cagaaagta aatcaaaac tgatgttat 
        ttcccttct ttcactcat ttttgcctc gatttttgc 
        tgtttgtgt gcgaaat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY