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Gene information
General information
TC014748 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14486723..14488476
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014748
    iBeetle screen iB_10470

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014748 RA
      MKKILYLFF AIFVTAIFR VSNAEETFS CSIEKQKGN 
      YLRIDEPVY DTDDEIIVP QSEEYYMRE VQQAFIFDY 
      FYSKQQYDD NGNKMDLGT NILQIPLRR IDEKREISK 
      AGNFYYYNF TITLHCNND DTVDTHTFV PIIDTNNHG 
      PFFGKPKYT YNLISSYAE DFKRQQLLN PIIATDYDV 
      TNTAVKFNI EENNHFDIM YNGIVPNSL NKQHYALLT 
      PKIPGKPIT DKIEVIVTV TDMGFPSRS NVTKVEINP 
      PSPPMPIFK SPFYVGHYY KNHTFVMHD NLELEEGCT 
      FYSTTVEIL VEDMRCQIF YATIVKDEI IINKSSKEY 
      VNNFYEENY TVYKLKAKY TNPITNLFT VGTATFILE 
      HHHDL
    • >TC014748 RB
      MESCKLSRN VTSTWPLCC SIEKQKGNY LRIDEPVYD 
      TDDEIIVPQ SEEYYMREV QQAFIFDYF YSKQQYDDN 
      GNKMDLGTN ILQIPLRRI DEKREISKA GNFYYYNFT 
      ITLHCNNDD TVDTHTFVP IIDTNNHGP FFGKPKYTY 
      NLISSYAED FKRQQLLNP IIATDYDVT NTAVKFNIE 
      ENNHFDIMY NGIVPNSLN KQHYALLTP KIPGKPITD 
      KIEVIVTVT DMGFPSRSN VTKVEINPP SPPMPIFKS 
      PFYVGHYYK NHTFVMHDN LELEEGCTF YSTTVEILV 
      EDMRCQIFY ATIVKDEII INKSSKEYV NNFYEENYT 
      VYKLKAKYT NPITNLFTV GTATFILEH HHDL
    CDS sequence
    • >TC014748 RA
      atgaaaaaa attttgtat ctgtttttc gctatcttt 
      gtgacagca atatttcga gtatcaaat gctgaggaa 
      acttttagt tgttcaatc gaaaaacag aaaggaaat 
      tacttgaga attgatgaa ccagtttac gatactgat 
      gatgaaatt attgtccct cagagcgaa gaatattat 
      atgagagag gtccaacaa gcttttatt tttgattat 
      ttttattct aaacaacaa tatgatgat aatggaaat 
      aagatggat ctcggaaca aatatcttg caaattcct 
      ttgagacga atagacgaa aagagagaa attagtaaa 
      gcaggcaat ttttattac tacaatttt acaataacg 
      ttacattgt aataatgat gatacggtt gacacgcac 
      acatttgtg cctattatt gacaccaac aaccacgga 
      ccatttttt ggcaaacca aaatacacc tacaacctc 
      atttcctca tacgctgaa gatttcaaa cgccaacaa 
      ctcctaaac cccattatc gcaaccgat tatgacgta 
      acaaacaca gcagtgaaa ttcaacatt gaagaaaac 
      aaccatttc gatataatg tacaatggc attgttcct 
      aattccctc aacaagcaa cactacgct ttactaacg 
      ccaaaaatc cctggcaaa cccattacc gataaaatt 
      gaagttatc gtgacagta actgatatg ggttttcct 
      tcgaggagt aacgttaca aaagttgag ataaatcca 
      ccatcgcca ccaatgcca atttttaaa tctcctttt 
      tacgttggt cattattac aaaaatcat acgtttgtc 
      atgcatgat aatctagag ttggaagaa ggatgcaca 
      ttttattcg acaactgtt gaaatttta gtcgaggat 
      atgagatgc caaatcttt tacgctaca atagtcaaa 
      gatgaaatt attataaac aaaagtagt aaagagtat 
      gtgaataat ttttacgaa gaaaactac acagtgtac 
      aaactgaaa gcaaaatat accaatccg atcaccaac 
      ctatttact gtcggaaca gcaactttc attctagaa 
      catcatcac gatttataa
    • >TC014748 RB
      atggagagt tgtaaactc agcagaaat gttacttca 
      acttggcct ttatgttgt tcaatcgaa aaacagaaa 
      ggaaattac ttgagaatt gatgaacca gtttacgat 
      actgatgat gaaattatt gtccctcag agcgaagaa 
      tattatatg agagaggtc caacaagct tttattttt 
      gattatttt tattctaaa caacaatat gatgataat 
      ggaaataag atggatctc ggaacaaat atcttgcaa 
      attcctttg agacgaata gacgaaaag agagaaatt 
      agtaaagca ggcaatttt tattactac aattttaca 
      ataacgtta cattgtaat aatgatgat acggttgac 
      acgcacaca tttgtgcct attattgac accaacaac 
      cacggacca ttttttggc aaaccaaaa tacacctac 
      aacctcatt tcctcatac gctgaagat ttcaaacgc 
      caacaactc ctaaacccc attatcgca accgattat 
      gacgtaaca aacacagca gtgaaattc aacattgaa 
      gaaaacaac catttcgat ataatgtac aatggcatt 
      gttcctaat tccctcaac aagcaacac tacgcttta 
      ctaacgcca aaaatccct ggcaaaccc attaccgat 
      aaaattgaa gttatcgtg acagtaact gatatgggt 
      tttccttcg aggagtaac gttacaaaa gttgagata 
      aatccacca tcgccacca atgccaatt tttaaatct 
      cctttttac gttggtcat tattacaaa aatcatacg 
      tttgtcatg catgataat ctagagttg gaagaagga 
      tgcacattt tattcgaca actgttgaa attttagtc 
      gaggatatg agatgccaa atcttttac gctacaata 
      gtcaaagat gaaattatt ataaacaaa agtagtaaa 
      gagtatgtg aataatttt tacgaagaa aactacaca 
      gtgtacaaa ctgaaagca aaatatacc aatccgatc 
      accaaccta tttactgtc ggaacagca actttcatt 
      ctagaacat catcacgat ttataa
    mRNA sequence
    • >TC014748 RA
      ccacgtaga aaatatttg actgtttgt taaaattgc 
      gaagtacat actcggacg ttactaaaa atgaaaaaa 
      attttgtat ctgtttttc gctatcttt gtgacagca 
      atatttcga gtatcaaat gctgaggaa acttttagt 
      tgttcaatc gaaaaacag aaaggaaat tacttgaga 
      attgatgaa ccagtttac gatactgat gatgaaatt 
      attgtccct cagagcgaa gaatattat atgagagag 
      gtccaacaa gcttttatt tttgattat ttttattct 
      aaacaacaa tatgatgat aatggaaat aagatggat 
      ctcggaaca aatatcttg caaattcct ttgagacga 
      atagacgaa aagagagaa attagtaaa gcaggcaat 
      ttttattac tacaatttt acaataacg ttacattgt 
      aataatgat gatacggtt gacacgcac acatttgtg 
      cctattatt gacaccaac aaccacgga ccatttttt 
      ggcaaacca aaatacacc tacaacctc atttcctca 
      tacgctgaa gatttcaaa cgccaacaa ctcctaaac 
      cccattatc gcaaccgat tatgacgta acaaacaca 
      gcagtgaaa ttcaacatt gaagaaaac aaccatttc 
      gatataatg tacaatggc attgttcct aattccctc 
      aacaagcaa cactacgct ttactaacg ccaaaaatc 
      cctggcaaa cccattacc gataaaatt gaagttatc 
      gtgacagta actgatatg ggttttcct tcgaggagt 
      aacgttaca aaagttgag ataaatcca ccatcgcca 
      ccaatgcca atttttaaa tctcctttt tacgttggt 
      cattattac aaaaatcat acgtttgtc atgcatgat 
      aatctagag ttggaagaa ggatgcaca ttttattcg 
      acaactgtt gaaatttta gtcgaggat atgagatgc 
      caaatcttt tacgctaca atagtcaaa gatgaaatt 
      attataaac aaaagtagt aaagagtat gtgaataat 
      ttttacgaa gaaaactac acagtgtac aaactgaaa 
      gcaaaatat accaatccg atcaccaac ctatttact 
      gtcggaaca gcaactttc attctagaa catcatcac 
      gatttataa gcacataaa taaatctaa taaataaaa 
      atcttgttt tatt
    • >TC014748 RB
      ctgtgaatg cagataaac tcgcggggg ttcacagaa 
      ccgaataga gataatgga gagttgtaa actcagcag 
      aaatgttac ttcaacttg gcctttatg ttgttcaat 
      cgaaaaaca gaaaggaaa ttacttgag aattgatga 
      accagttta cgatactga tgatgaaat tattgtccc 
      tcagagcga agaatatta tatgagaga ggtccaaca 
      agcttttat ttttgatta tttttattc taaacaaca 
      atatgatga taatggaaa taagatgga tctcggaac 
      aaatatctt gcaaattcc tttgagacg aatagacga 
      aaagagaga aattagtaa agcaggcaa tttttatta 
      ctacaattt tacaataac gttacattg taataatga 
      tgatacggt tgacacgca cacatttgt gcctattat 
      tgacaccaa caaccacgg accattttt tggcaaacc 
      aaaatacac ctacaacct catttcctc atacgctga 
      agatttcaa acgccaaca actcctaaa ccccattat 
      cgcaaccga ttatgacgt aacaaacac agcagtgaa 
      attcaacat tgaagaaaa caaccattt cgatataat 
      gtacaatgg cattgttcc taattccct caacaagca 
      acactacgc tttactaac gccaaaaat ccctggcaa 
      acccattac cgataaaat tgaagttat cgtgacagt 
      aactgatat gggttttcc ttcgaggag taacgttac 
      aaaagttga gataaatcc accatcgcc accaatgcc 
      aatttttaa atctccttt ttacgttgg tcattatta 
      caaaaatca tacgtttgt catgcatga taatctaga 
      gttggaaga aggatgcac attttattc gacaactgt 
      tgaaatttt agtcgagga tatgagatg ccaaatctt 
      ttacgctac aatagtcaa agatgaaat tattataaa 
      caaaagtag taaagagta tgtgaataa tttttacga 
      agaaaacta cacagtgta caaactgaa agcaaaata 
      taccaatcc gatcaccaa cctatttac tgtcggaac 
      agcaacttt cattctaga acatcatca cgatttata 
      agcacataa ataaatcta ataaataaa aatcttgtt 
      ttatt
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