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Gene information
General information
TC005831 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7105275..7106719
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005831
    iBeetle screen iB_10459

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005831 RA
      MLRNIGLVF RGFEVKFST NASRVSVPH EDIATKVLK 
      VAVIGVPNV GKSTFINNL MDRKVCPAS SKVHTTRAK 
      SQAIFTEGD SQIVFLDTP GLVSFQEYK KFSLEKSFL 
      KDSKSSLRE ADIIGVIHD ASNIWTREK LDIKVIRLL 
      EDNKTKPSF LVLNKVDII KSKRKLLDV TRQLTENCI 
      EGAPIPGGK PLRDKETRG WPHFSDVFM VSALTGSGL 
      DEVKNYLIR NAKPGEWPY PGEIWSDKT AEEIIVDSV 
      KAKLLDFLP QEIPYTLKP EMEFFNIND KGVISAVVL 
      VHCPSKRIA QLVAGESEG KLRQINESL QVDLQRTFH 
      NYVRIKVVL SDK
    CDS sequence
    • >TC005831 RA
      atgttgcga aacatcggc ttagttttt cgcggattt 
      gaggtaaaa ttcagcaca aatgcctcg cgagtgtca 
      gtcccacac gaagacatt gcgaccaaa gtgttgaag 
      gtggcagtg ataggtgtc ccaaatgtg ggcaagagc 
      actttcatc aacaactta atggaccga aaggtttgc 
      ccagcgtct agtaaagtc cacacgaca cgtgccaaa 
      tcccaggcg attttcaca gaaggggat tcccaaatc 
      gtctttctg gacactcct ggccttgtc agtttccag 
      gaatacaaa aaattcagt ttggagaaa tcattttta 
      aaggacagc aaatcgtcg ttacgcgag gccgacata 
      attggggtc atccacgat gctagtaat atctggacg 
      cgtgaaaaa ctcgacatt aaagtgatt cgtttactc 
      gaagacaac aaaaccaag cccagtttt ctagtttta 
      aataaagtc gacattatc aaatccaag cgaaagctg 
      cttgatgtc actcgccaa ttgacggaa aattgcatc 
      gagggggcc cccattccg gggggcaaa ccactaagg 
      gacaaagaa acgcggggg tggccccac tttagcgac 
      gtttttatg gtctcagcc ctcacagga agcggttta 
      gacgaagtt aaaaactat ttaattcgc aatgcaaaa 
      ccaggcgag tggccttat ccgggggaa atctggtcg 
      gataagacc gccgaagag atcattgtt gacagcgtc 
      aaggcgaaa ttactggat tttttgcca caggaaatc 
      ccctacact ctcaaaccc gaaatggag tttttcaat 
      attaacgac aaaggggtg atttcggcc gttgtcttg 
      gtgcactgc cccagcaaa cgcattgcg cagcttgtg 
      gcaggggaa agcgaggga aaattgcgc cagatcaat 
      gaaagtctc caagttgat ttacaaaga acgtttcat 
      aattatgtg agaattaag gtcgttctg agcgataaa 
      tag
    mRNA sequence
    • >TC005831 RA
      tagctaaac atggtttat tttatgagt tgttgcaaa 
      cataacctc actcttcct aacgaaatg ttgcgaaac 
      atcggctta gtttttcgc ggatttgag gtaaaattc 
      agcacaaat gcctcgcga gtgtcagtc ccacacgaa 
      gacattgcg accaaagtg ttgaaggtg gcagtgata 
      ggtgtccca aatgtgggc aagagcact ttcatcaac 
      aacttaatg gaccgaaag gtttgccca gcgtctagt 
      aaagtccac acgacacgt gccaaatcc caggcgatt 
      ttcacagaa ggggattcc caaatcgtc tttctggac 
      actcctggc cttgtcagt ttccaggaa tacaaaaaa 
      ttcagtttg gagaaatca tttttaaag gacagcaaa 
      tcgtcgtta cgcgaggcc gacataatt ggggtcatc 
      cacgatgct agtaatatc tggacgcgt gaaaaactc 
      gacattaaa gtgattcgt ttactcgaa gacaacaaa 
      accaagccc agttttcta gttttaaat aaagtcgac 
      attatcaaa tccaagcga aagctgctt gatgtcact 
      cgccaattg acggaaaat tgcatcgag ggggccccc 
      attccgggg ggcaaacca ctaagggac aaagaaacg 
      cgggggtgg ccccacttt agcgacgtt tttatggtc 
      tcagccctc acaggaagc ggtttagac gaagttaaa 
      aactattta attcgcaat gcaaaacca ggcgagtgg 
      ccttatccg ggggaaatc tggtcggat aagaccgcc 
      gaagagatc attgttgac agcgtcaag gcgaaatta 
      ctggatttt ttgccacag gaaatcccc tacactctc 
      aaacccgaa atggagttt ttcaatatt aacgacaaa 
      ggggtgatt tcggccgtt gtcttggtg cactgcccc 
      agcaaacgc attgcgcag cttgtggca ggggaaagc 
      gagggaaaa ttgcgccag atcaatgaa agtctccaa 
      gttgattta caaagaacg tttcataat tatgtgaga 
      attaaggtc gttctgagc gataaatag aaaaacatt 
      gaaaaatac agttgctct ttcttagag gtaaggacc 
      ccagcctat gtacatttg gcacaaatt atcgatatt 
      cttcgcttc atttattaa actattcgt ttgtccgtc 
      gactgataa aggtattga caatgagcg gtccttcgt 
      tttcacgat aattccttg caacctcag ttggatgtt 
      tttgacatg gtctagggc ctgaaaaag aaaaaacat 
      tgatgata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY