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Gene information
General information
TC006298 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6683342..6690926
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006298
    iBeetle screen iB_10457

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006298 RA
      MILSRVKPA ISASSDPGT SVRDNSTNV PKFEDYLKK 
      RDYTGAMTL LEFMQIQEP DVNIDLWMG YCAFHQGNY 
      KKALTIYEN LLKSKPDLD NLAVNLACC YFYLGMYEE 
      SKEILQKSG PSGLKTRLN FHLSHKLRD EAALMEHHE 
      QLQDVLEDQ LSLAAIHYL RAHYQEAID IYKRLLLQN 
      RNNLALNVY VALCYYKLD YYDVSQEVL TLYLNQYPD 
      SVIATNLKA CNHFRLYKG SVAENELRS IVEQSANVG 
      FGYDLVKHN LVVFRDGEG AMQVFPSLV DVIPEARLN 
      LVIHYLRND DIKEAYDLL QDLQPAVPQ EYILKGVVN 
      AALGQELNS PDHIKTAEE CFQLVGSSA SECDTIDGR 
      QCMAAFFFL AGQFEEVLV YLSSIKSFL HSDDTFNFN 
      FAQAKTACS QFKEAEETF LLIQDPKIK ESPIYISNL 
      ARCYVMNGR PELAWELYL KMESSPESF NLLLLIAND 
      CYKMGQFWY AAKAFDMLD RLDPNPEFW EGKRGAIAG 
      VFQGVVAQK FPVDMLGDV LQLLRSSTS TQADQMAKV 
      IRQWVKEKR VTVS
    CDS sequence
    • >TC006298 RA
      atgatttta agcagagtt aagccggct ataagtgcc 
      tcaagtgac cccggcact tcagttcgt gacaattcc 
      accaacgtc ccgaaattc gaggattac ctcaaaaag 
      cgggactac accggcgct atgacctta ctagaattt 
      atgcaaata caagagccc gacgtgaac atagacctt 
      tggatgggt tactgcgct ttccaccaa ggaaattac 
      aaaaaagcg ctaactatt tatgaaaat ttgctgaaa 
      tcgaagcca gatctggac aaccttgcg gtgaatttg 
      gcttgttgt tacttctac ttggggatg tacgaagaa 
      tcgaaagag attttgcaa aaatcgggg cccagtggg 
      ctaaaaacg cgcctaaac tttcatttg tctcacaaa 
      ttgcgggac gaggcggct ttgatggag caccacgag 
      cagttgcag gacgtcctc gaggaccag ttaagtctg 
      gcggctata cactacttg agggctcat taccaagaa 
      gccatcgat atttacaaa cggttattg ctgcaaaat 
      cgtaataat ctagccctg aatgtttac gtggcgctc 
      tgttactac aagcttgat tattacgac gtgtctcag 
      gaagttctg actttgtat ctcaaccag tacccggac 
      tcggtcatc gcaacaaac ttaaaagcc tgtaaccat 
      ttccggctt tacaaaggc tcagttgcc gaaaacgaa 
      ttgagatca atcgtcgaa caaagcgca aatgttggc 
      ttcgggtac gatctagtc aaacacaat ctagttgtg 
      ttccgagat ggcgaaggc gctatgcaa gtgtttcca 
      tcgctggtt gacgtaatc ccagaagcg aggctgaat 
      cttgtcata cattattta agaaatgac gatattaag 
      gaagcttat gatctgttg caagattta caaccggcc 
      gtgccccag gagtacatc ctcaaaggg gttgtcaat 
      gcggcctta ggacaggag ttgaactct cctgatcat 
      attaaaaca gcagaggag tgtttccag ttagttggg 
      agttcggcg agtgaatgt gatacgatc gatgggcgg 
      caatgtatg gccgcattt ttctttcta gcgggacag 
      tttgaggaa gtcctcgtt tatttgtcc tcaatcaaa 
      agctttctc cactcagac gatacattt aatttcaac 
      ttcgcccaa gccaagacc gcttgcagc cagttcaag 
      gaggccgag gagacgttt cttctgatt caggacccg 
      aagataaag gagagtccg atttatatt tccaacttg 
      gctcggtgt tacgtcatg aatggacgg cccgaactg 
      gcatgggag ctttatttg aagatggaa agtagtccc 
      gagagcttc aatttgttg cttttgatt gccaatgat 
      tgctacaag atggggcag ttctggtac gccgccaag 
      gccttcgat atgttggac agactcgac cccaatccg 
      gagttttgg gagggaaaa aggggcgca attgcgggg 
      gttttccaa ggggtggtg gcccagaag ttcccagtg 
      gacatgctg ggtgatgtg ttgcagcta ctgaggagc 
      agcacctcg acccaggct gaccaaatg gccaaagta 
      ataagacag tgggtgaag gagaaaagg gtcacggtt 
      tcataa
    mRNA sequence
    • >TC006298 RA
      gtttctttc ccaacaatg attttaagc agagttaag 
      ccggctata agtgcctca agtgacccc ggcacttca 
      gttcgtgac aattccacc aacgtcccg aaattcgag 
      gattacctc aaaaagcgg gactacacc ggcgctatg 
      accttacta gaatttatg caaatacaa gagcccgac 
      gtgaacata gacctttgg atgggttac tgcgctttc 
      caccaagga aattacaaa aaagcgcta actatttat 
      gaaaatttg ctgaaatcg aagccagat ctggacaac 
      cttgcggtg aatttggct tgttgttac ttctacttg 
      gggatgtac gaagaatcg aaagagatt ttgcaaaaa 
      tcggggccc agtgggcta aaaacgcgc ctaaacttt 
      catttgtct cacaaattg cgggacgag gcggctttg 
      atggagcac cacgagcag ttgcaggac gtcctcgag 
      gaccagtta agtctggcg gctatacac tacttgagg 
      gctcattac caagaagcc atcgatatt tacaaacgg 
      ttattgctg caaaatcgt aataatcta gccctgaat 
      gtttacgtg gcgctctgt tactacaag cttgattat 
      tacgacgtg tctcaggaa gttctgact ttgtatctc 
      aaccagtac ccggactcg gtcatcgca acaaactta 
      aaagcctgt aaccatttc cggctttac aaaggctca 
      gttgccgaa aacgaattg agatcaatc gtcgaacaa 
      agcgcaaat gttggcttc gggtacgat ctagtcaaa 
      cacaatcta gttgtgttc cgagatggc gaaggcgct 
      atgcaagtg tttccatcg ctggttgac gtaatccca 
      gaagcgagg ctgaatctt gtcatacat tatttaaga 
      aatgacgat attaaggaa gcttatgat ctgttgcaa 
      gatttacaa ccggccgtg ccccaggag tacatcctc 
      aaaggggtt gtcaatgcg gccttagga caggagttg 
      aactctcct gatcatatt aaaacagca gaggagtgt 
      ttccagtta gttgggagt tcggcgagt gaatgtgat 
      acgatcgat gggcggcaa tgtatggcc gcatttttc 
      tttctagcg ggacagttt gaggaagtc ctcgtttat 
      ttgtcctca atcaaaagc tttctccac tcagacgat 
      acatttaat ttcaacttc gcccaagcc aagaccgct 
      tgcagccag ttcaaggag gccgaggag acgtttctt 
      ctgattcag gacccgaag ataaaggag agtccgatt 
      tatatttcc aacttggct cggtgttac gtcatgaat 
      ggacggccc gaactggca tgggagctt tatttgaag 
      atggaaagt agtcccgag agcttcaat ttgttgctt 
      ttgattgcc aatgattgc tacaagatg gggcagttc 
      tggtacgcc gccaaggcc ttcgatatg ttggacaga 
      ctcgacccc aatccggag ttttgggag ggaaaaagg 
      ggcgcaatt gcgggggtt ttccaaggg gtggtggcc 
      cagaagttc ccagtggac atgctgggt gatgtgttg 
      cagctactg aggagcagc acctcgacc caggctgac 
      caaatggcc aaagtaata agacagtgg gtgaaggag 
      aaaagggtc acggtttca taataaata agccccctt 
      ttagtcgct gtattttct ctaatttaa ttctagcaa 
      taaattgtg ttttttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY