Status
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Gene information
General information
TC034020
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005866
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6182589..6189620
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034020
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10456

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034020 RA
      MIGGLEPIE SNLHRHLIE HLNAEVVLQ TITGLEVAL 
      RWLTSTFLY IRAKKNPRH YGLPPSSDP AAVDRRLLE 
      MCQIELNKL IKAGMLTID QDVLLKATP VGAAMAKYY 
      LAFETMKLF TQINGGEIL QQILHLISK CSEFSEMYL 
      RVNDKKCLN LLNKCRNRQ TIRFPLNGK IKTLDMKIN 
      CIIQAVLGC LDICDHSIL SETLKIMRN GERIVKCLI 
      EYLESKEKC FQALLSTII LAKCFHVKL WENSPFVSK 
      QLPGIGNVM SSQLVNAGK TSFQLISEC EAREIELLI 
      NKKPPAGNK ILEQIQHLP KYEMELQVH SNTQIKLAI 
      SLMNAQDLE TKCTVNRNS LMYLLVGDS SNNILLYEK 
      YSHSYFIEN PDVVRFVDL HEKNLDVIE AHFISEDWV 
      GIDCTAKYE TAQVVRKPE PKTLKKNTE DPTYMQTFI 
      DMYMKCKKN IEPPNPAKK AKIETNEDR NANKIIIHS 
      DISLNNNNT SEEKIVNNS VFRPSPAPE DKIEKIPVL 
      VASRYDLSP SRDKNELNV NNDYQISLK PECEDAKSI 
      VKKFYYTPK KLSHKKEFS WNESEDKPK STTKITWRS 
      PLTYSPSVK FSPKIGYNR PNENECLST SRASSSSTN 
      QNIETMSNK TQKSNFANS RTPVKLNSS QQKVVFPLT 
      DKDCANLSM EENSRRNDI LEKVGDFAS ISLFGLVSP 
      TKRQEKFSQ IQTPNQYYN QLLSQSEKT ESTPKKNKI 
      FEKPKLVEN VVKEVCDRS RYFENDSNF CLTPRRNNG 
      ARNSENLEY TTTMTESGE KNNTENMNI FEDPFNDSL 
      ESALMKFDC GPPSELESD KSNSFDKEG LFENEAKEV 
      RHPFDCREP SPPEMLRKN TNQICRSRF FQPMAEPSH 
      YQNRSDDSF LTQKSHPYP YQSRAPAHM FPISNGRGF 
      ETPNDFYKM MCEERPINR HYVDLGQNP YLNISQDFP 
      DRREHYNSQ RHLFDGTPR CYPRGHMDD CDGYAYIRR 
      NMDRDYDPF DYRNNPQYY KPVPNIDFY ERSQRSFAR 
      NRENIPFQD FDLDRSNSY YDESHLPLS YPELPSSQM 
      SRREYYMPS NSFNDVYNN KQLIMQRFL NCVGSDQEK 
      RVNYFP
    CDS sequence
    • >TC034020 RA
      atgatcggt ggtttagaa cccatcgaa tcgaatttg 
      caccgacat ttaatcgag catttgaat gctgaggtg 
      gttttgcag accatcaca ggccttgaa gttgcacta 
      cgttggctt acttccact tttctctac attcgagcg 
      aagaaaaat ccgagacat tacggccta ccaccgagc 
      tcggatccg gctgcggta gaccggagg cttctagag 
      atgtgtcaa atcgagctg aataaatta attaaagcg 
      ggaatgtta acaatagat caagacgtt cttctcaag 
      gcgactccg gttggggcg gcaatggct aaatattac 
      ctcgccttc gaaactatg aaattgttt acacagata 
      aatggaggc gaaattttg caacaaatc ctgcatttg 
      atctccaag tgttctgag ttctccgaa atgtatttg 
      cgagtcaac gataaaaaa tgcctgaat ttgttaaac 
      aagtgccgc aatagacaa acaataaga tttccactg 
      aacggcaaa atcaagact ttggacatg aaaattaat 
      tgtattatt caagcagtt ttgggctgt ctggacatt 
      tgtgaccac tcgattctc tcagaaacg ttaaaaatc 
      atgcgaaat ggcgaaaga atcgtcaaa tgtttgatt 
      gaatacctg gaatcgaag gaaaaatgt ttccaggca 
      ttgctcagc acaattatt ttagcaaaa tgctttcat 
      gtaaaacta tgggaaaat tcgccattt gttagcaaa 
      caattgcct ggaattgga aatgtgatg tcatctcaa 
      ttggtcaat gccggcaaa acctccttt caactaata 
      tcagagtgc gaagcgcga gaaattgaa ttattaatt 
      aacaaaaaa ccaccagct ggtaacaaa attctggaa 
      caaatccag catttacca aaatacgaa atggaacta 
      caagtgcac agtaacacc caaataaaa ttggcgatt 
      tcgctaatg aatgcgcaa gatttggaa actaagtgc 
      accgtcaat cgaaattca ttgatgtat cttttggtt 
      ggtgattct tccaataat attttgctt tacgaaaag 
      tacagtcac tcctacttt attgaaaac ccggacgtt 
      gtccgattt gtggatttg cacgaaaaa aatctggac 
      gtaattgaa gctcacttc attagtgag gattgggtg 
      ggcatcgat tgtaccgcg aaatacgaa acggcccaa 
      gtcgttcgt aaacccgaa cctaaaact ctgaaaaag 
      aataccgaa gatccgact tacatgcaa acttttatc 
      gacatgtac atgaagtgc aaaaaaaat atagagcca 
      ccgaatccg gcaaaaaag gccaaaatt gagacgaat 
      gaagatcga aatgcgaac aagattata atccatagt 
      gacataagt ttaaacaat aataacaca agtgaggaa 
      aaaatcgta aacaattca gtttttcgt ccttcaccg 
      gcacctgaa gacaaaatt gaaaagatt cccgttttg 
      gttgcttca agatatgat ctttcgccg tcacgtgac 
      aaaaatgaa ttgaacgtc aacaatgac taccaaatt 
      agtttaaaa cctgaatgt gaagatgcc aaatctatc 
      gtgaaaaag ttttactac acccctaaa aagttgtct 
      cataaaaaa gaattttct tggaacgaa agtgaagat 
      aaacctaaa agtaccact aaaataacg tggaggtcg 
      cccttgaca tactctccc tcggttaaa ttctcgccg 
      aagatcggt tataaccgg ccgaatgag aatgaatgc 
      ttgtcaact agtcgagct tcatcgtca tctacaaac 
      caaaatatt gaaacgatg tcaaacaaa actcaaaag 
      tcgaatttt gcaaattct cgaactccg gttaagtta 
      aattcgtcg caacagaag gtcgtgttt ccattgact 
      gataaagac tgtgccaac ttatcaatg gaagagaac 
      agtagacga aatgatatt ttggagaaa gttggtgat 
      tttgcgtcc atttcgctc ttcggtctc gtatcacca 
      accaaacgg caagaaaaa tttagtcaa attcagacc 
      ccgaatcag tattacaat caactattg tcacaatcg 
      gaaaagact gaaagtact cccaagaaa aacaaaatt 
      tttgaaaaa cccaagttg gtggaaaat gtggttaaa 
      gaagtttgc gaccgaagc cgttatttt gaaaatgat 
      tctaacttt tgtctgaca cctcgtcgt aataatggg 
      gcacgtaat tcagaaaat ttggaatat acaacaaca 
      atgaccgaa agtggggaa aaaaacaac actgaaaat 
      atgaacata tttgaggat ccttttaac gattccctt 
      gagtcggct ttgatgaaa ttcgattgt ggtccacct 
      tcggagcta gaaagtgat aaatcaaac agttttgat 
      aaagaaggt ctttttgaa aatgaggcg aaggaagtg 
      agacatcct tttgattgt cgagaaccg tccccgcct 
      gaaatgcta aggaaaaat acaaaccag atttgtcga 
      agtcggttc tttcaacca atggctgag cccagtcat 
      tatcaaaac cgttccgat gatagtttt ctgacacaa 
      aagtcacac ccttacccg taccaaagc cgtgcacct 
      gcacatatg tttccaata agtaatggt cgaggtttt 
      gagacaccg aatgatttt tacaagatg atgtgtgaa 
      gagcgtcct attaatcgt cattatgtg gatttgggt 
      caaaatcct tatttaaac atttcacaa gattttccc 
      gacaggagg gaacactac aacagtcag agacacctc 
      tttgatggt actcctcga tgttatccc agggggcat 
      atggatgat tgtgatggt tatgcctat attagacga 
      aacatggat agggattat gaccccttt gactatagg 
      aataatcct cagtactat aaaccagta cctaatatc 
      gatttctat gagaggtca caaaggagt tttgcccga 
      aatcgggaa aatattccc tttcaggat tttgatctt 
      gatcgaagt aatagttat tatgatgaa agtcattta 
      cctttgtct tatcccgaa ttgcccagt tcccagatg 
      tcaagacga gagtattat atgccgtct aattcattt 
      aacgatgtg tataacaat aagcagttg ataatgcaa 
      agattctta aattgtgta ggttcagac caagaaaaa 
      agagtgaat tatttccct taa
    mRNA sequence
    • >TC034020 RA
      gaaccgata taaaaaacg aaaattaga ccaaatctc 
      tgccggatt ggcagcgga ccaccagtt tggacaaat 
      caggaaata aacaatccc gaatatttt tttaaacaa 
      aaaccgcct tgcgttaat gaattaccg ccaattata 
      ataataaag cgctgaata attgtaacg cgtgtataa 
      taaatgttg cattgtgtg tgctccccg agcgtaacg 
      tcgattttt tcaggagaa gtttgaaaa aatgatcgg 
      tggtttaga acccatcga atcgaattt gcaccgaca 
      tttaatcga gcatttgaa tgctgaggt ggttttgca 
      gaccatcac aggccttga agttgcact acgttggct 
      tacttccac ttttctcta cattcgagc gaagaaaaa 
      tccgagaca ttacggcct accaccgag ctcggatcc 
      ggctgcggt agaccggag gcttctaga gatgtgtca 
      aatcgagct gaataaatt aattaaagc gggaatgtt 
      aacaataga tcaagacgt tcttctcaa ggcgactcc 
      ggttggggc ggcaatggc taaatatta cctcgcctt 
      cgaaactat gaaattgtt tacacagat aaatggagg 
      cgaaatttt gcaacaaat cctgcattt gatctccaa 
      gtgttctga gttctccga aatgtattt gcgagtcaa 
      cgataaaaa atgcctgaa tttgttaaa caagtgccg 
      caatagaca aacaataag atttccact gaacggcaa 
      aatcaagac tttggacat gaaaattaa ttgtattat 
      tcaagcagt tttgggctg tctggacat ttgtgacca 
      ctcgattct ctcagaaac gttaaaaat catgcgaaa 
      tggcgaaag aatcgtcaa atgtttgat tgaatacct 
      ggaatcgaa ggaaaaatg tttccaggc attgctcag 
      cacaattat tttagcaaa atgctttca tgtaaaact 
      atgggaaaa ttcgccatt tgttagcaa acaattgcc 
      tggaattgg aaatgtgat gtcatctca attggtcaa 
      tgccggcaa aacctcctt tcaactaat atcagagtg 
      cgaagcgcg agaaattga attattaat taacaaaaa 
      accaccagc tggtaacaa aattctgga acaaatcca 
      gcatttacc aaaatacga aatggaact acaagtgca 
      cagtaacac ccaaataaa attggcgat ttcgctaat 
      gaatgcgca agatttgga aactaagtg caccgtcaa 
      tcgaaattc attgatgta tcttttggt tggtgattc 
      ttccaataa tattttgct ttacgaaaa gtacagtca 
      ctcctactt tattgaaaa cccggacgt tgtccgatt 
      tgtggattt gcacgaaaa aaatctgga cgtaattga 
      agctcactt cattagtga ggattgggt gggcatcga 
      ttgtaccgc gaaatacga aacggccca agtcgttcg 
      taaacccga acctaaaac tctgaaaaa gaataccga 
      agatccgac ttacatgca aacttttat cgacatgta 
      catgaagtg caaaaaaaa tatagagcc accgaatcc 
      ggcaaaaaa ggccaaaat tgagacgaa tgaagatcg 
      aaatgcgaa caagattat aatccatag tgacataag 
      tttaaacaa taataacac aagtgagga aaaaatcgt 
      aaacaattc agtttttcg tccttcacc ggcacctga 
      agacaaaat tgaaaagat tcccgtttt ggttgcttc 
      aagatatga tctttcgcc gtcacgtga caaaaatga 
      attgaacgt caacaatga ctaccaaat tagtttaaa 
      acctgaatg tgaagatgc caaatctat cgtgaaaaa 
      gttttacta cacccctaa aaagttgtc tcataaaaa 
      agaattttc ttggaacga aagtgaaga taaacctaa 
      aagtaccac taaaataac gtggaggtc gcccttgac 
      atactctcc ctcggttaa attctcgcc gaagatcgg 
      ttataaccg gccgaatga gaatgaatg cttgtcaac 
      tagtcgagc ttcatcgtc atctacaaa ccaaaatat 
      tgaaacgat gtcaaacaa aactcaaaa gtcgaattt 
      tgcaaattc tcgaactcc ggttaagtt aaattcgtc 
      gcaacagaa ggtcgtgtt tccattgac tgataaaga 
      ctgtgccaa cttatcaat ggaagagaa cagtagacg 
      aaatgatat tttggagaa agttggtga ttttgcgtc 
      catttcgct cttcggtct cgtatcacc aaccaaacg 
      gcaagaaaa atttagtca aattcagac cccgaatca 
      gtattacaa tcaactatt gtcacaatc ggaaaagac 
      tgaaagtac tcccaagaa aaacaaaat ttttgaaaa 
      acccaagtt ggtggaaaa tgtggttaa agaagtttg 
      cgaccgaag ccgttattt tgaaaatga ttctaactt 
      ttgtctgac acctcgtcg taataatgg ggcacgtaa 
      ttcagaaaa tttggaata tacaacaac aatgaccga 
      aagtgggga aaaaaacaa cactgaaaa tatgaacat 
      atttgagga tccttttaa cgattccct tgagtcggc 
      tttgatgaa attcgattg tggtccacc ttcggagct 
      agaaagtga taaatcaaa cagttttga taaagaagg 
      tctttttga aaatgaggc gaaggaagt gagacatcc 
      ttttgattg tcgagaacc gtccccgcc tgaaatgct 
      aaggaaaaa tacaaacca gatttgtcg aagtcggtt 
      ctttcaacc aatggctga gcccagtca ttatcaaaa 
      ccgttccga tgatagttt tctgacaca aaagtcaca 
      cccttaccc gtaccaaag ccgtgcacc tgcacatat 
      gtttccaat aagtaatgg tcgaggttt tgagacacc 
      gaatgattt ttacaagat gatgtgtga agagcgtcc 
      tattaatcg tcattatgt ggatttggg tcaaaatcc 
      ttatttaaa catttcaca agattttcc cgacaggag 
      ggaacacta caacagtca gagacacct ctttgatgg 
      tactcctcg atgttatcc cagggggca tatggatga 
      ttgtgatgg ttatgccta tattagacg aaacatgga 
      tagggatta tgacccctt tgactatag gaataatcc 
      tcagtacta taaaccagt acctaatat cgatttcta 
      tgagaggtc acaaaggag ttttgcccg aaatcggga 
      aaatattcc ctttcagga ttttgatct tgatcgaag 
      taatagtta ttatgatga aagtcattt acctttgtc 
      ttatcccga attgcccag ttcccagat gtcaagacg 
      agagtatta tatgccgtc taattcatt taacgatgt 
      gtataacaa taagcagtt gataatgca aagattctt 
      aaattgtgt aggttcaga ccaagaaaa aagagtgaa 
      ttatttccc ttaaaaaac agctttcga gttactttt 
      gttacggtt gtcgaataa acgacattt tgataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY