Status
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Gene information
General information
TC034013 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5952225..5980143
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034013
    iBeetle screen iB_10452

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034013 RA
      MSGSEGGVV RSGYLKKLK TSRKKFFVL RAETADASA 
      RLEYYDSER KFNNGLPPK RSIPLKNCF NINKRLDTK 
      HKHVIALYT KDDCFCVVL DNDDDLDSW LKALLKLQH 
      GEEVVDGET PKPTFEHVW QVTVLNRGL GNAGLTGNY 
      RLCLTDKTL SLIKRDCEK PTIELNLSN IRSCGNLRD 
      FFFLEIGRS STLGAGELW MQTEDNNIA QNVHSTVFH 
      AMSTNSSSK DELGPKSRN RSSSATESS KPNMTRKIH 
      IAPKCHIFL PPDVFHTSD TQVIPAGSS TQHPLVSGV 
      ASFYQHLIQ RGAKASERR HSISAGSLT HCGVISHQR 
      TQSLPLANP APLSDHSHS RKRPKCMSR ERCDSMPSR 
      ARTTSEGNH RHLNPYRPA SLYGRDISH SPPLGSPVS 
      PPCSTDSAG SSYSLTDET DVCTEMEPS LGRYGHSLT 
      PDEAIAEED CPDSPPYNN YISMTLHSS DDGYVDMSP 
      RGRHHNSPT ASMSSVTSG TPSTDMRFA EYPLEKVTS 
      YLADDDGRP ARAYSVGSK PERYKKYTE MGGNSDNLR 
      ARALSVGSK KPPARVLPP HGYHHPGTK SSSAPILTN 
      SRGQGSYNS IGPMDDLME MDFSHNSGY MDMKPVNSG 
      YVEMKPGRK PETSPYVDM SSGSSPAKP SYISPAATT 
      TQTEVYMEM DPRKNHSEY LSMSFTKKL PSPGSPGTP 
      EGYVEMSLG RGHNPSQLT EDYANMSLN NTKKRPNRK 
      KDKNRSEPI NIQGGPNPR SLLLARKHS TGTPPTMYL 
      PLVEATPHD SKDSSSSSI NTPSSSSTI FPISLNSPS 
      KATPDYIPM DFDKSDYVN YDPKPGVGE FRPINESND 
      DEGRAYEVL RPSSSESCS TIGGSRPAS VSSAHMHYA 
      SLDLDEGGN RSPRTVKGS GGEQGEVTL TYAAIDFVK 
      SEGLKHNAV AATNAKVKH
    CDS sequence
    • >TC034013 RA
      atgtcgggc agtgagggt ggagtggtc cgcagtggc 
      tacctcaaa aaactcaaa acctcccgc aagaagttc 
      ttcgtgctg cgagccgag acggcggac gcgtccgcc 
      aggctggag tactacgac tcggagagg aagttcaac 
      aatgggctg cccccgaag cggagcatc ccgctgaag 
      aattgcttc aatatcaac aaacggctg gacaccaaa 
      cataaacac gtcattgcg ttgtacacg aaggacgac 
      tgcttctgc gtcgtcttg gacaacgac gacgacttg 
      gacagctgg ttgaaggcg cttttgaag ctccaacac 
      ggagaagaa gtcgtcgac ggggagaca cccaagccc 
      acgttcgag cacgtgtgg caagtgacc gtacttaat 
      cgaggcctc ggaaatgcc ggccttacc ggaaattac 
      cgcctctgt cttaccgac aagactctg agtctgatc 
      aaaagagac tgcgaaaaa ccaacaatc gaactgaac 
      ctttcgaat attcgaagt tgcggaaat ctccgcgat 
      tttttcttc ttggagatt ggtagatca agtaccttg 
      ggagccggc gaattatgg atgcaaacg gaagacaac 
      aacattgcc caaaatgtg cattcgacc gtttttcat 
      gccatgtct accaacagc agcagcaag gacgaattg 
      ggcccgaaa agccgtaat cgttcctct tcggccacc 
      gaatcctcc aaacctaac atgacgcga aaaatccac 
      atcgccccc aaatgtcat atatttcta ccaccagat 
      gtgttccac actagtgac actcaggtc attcccgcg 
      ggcagctcc acgcagcac cctctcgtg agcggtgtt 
      gccagtttt tatcaacat ctaatccag agaggagcc 
      aaagcttca gaacgtcga cattcaata tcagcaggt 
      agtctaacc cattgtggc gtcatcagc catcagcgc 
      actcaatca ctgccttta gcgaatccc gcacctctt 
      tccgatcac tcgcactca cgaaaacgc cccaaatgt 
      atgagccgg gaacgttgc gattcgatg ccatcccgg 
      gcacgaacc acatccgag gggaatcac cgacacttg 
      aatccttat cggccggca tcgctgtac ggtcgtgac 
      atttcgcac agtccgcca ttaggcagt ccggtgagt 
      cccccgtgt tccaccgat tcggcagga agttcgtac 
      agtttgacc gacgaaacg gacgtttgt acggaaatg 
      gaaccaagt ttgggacgc tatggacac tcattgacc 
      ccagacgag gcgattgcc gaggaggac tgtcctgat 
      agccccccc tacaataat tacatttcg atgacgttg 
      cacagctcg gacgatgga tatgtggat atgtcgccc 
      aggggacgg catcataat tcgccgacg gcgagtatg 
      agtagcgtc acttcgggg actccctca acggacatg 
      cgcttcgcg gagtatccc cttgagaag gtgacgtcg 
      tacttggcc gacgatgac ggacggcca gctcgggca 
      tattcggtc ggttccaaa ccggaacgg tataaaaaa 
      tacacggaa atgggcgga aacagcgat aatttacgg 
      gcacgtgcg ttaagtgtt gggtcgaaa aaaccacca 
      gctagggtt ttaccaccg catgggtac caccatcct 
      ggtaccaag tcgtcaagt gcgcccatc ttaaccaat 
      tcccgcggt cagggctcg tacaattcc atcggccca 
      atggacgat ctcatggaa atggatttc agtcataac 
      tccggttac atggacatg aagccggtc aactccggt 
      tacgtggaa atgaaaccg ggccgtaaa ccggaaacc 
      tcaccttac gtcgacatg tcgtccggt tcttccccg 
      gccaaaccg tcgtacatt tctcccgcc gccaccacg 
      acccaaacc gaagtctac atggagatg gaccctcga 
      aaaaatcac agcgagtat ttatcgatg agttttact 
      aaaaaactc ccaagtccg gggagtccc gggaccccc 
      gaaggctac gtcgagatg tcgctcggg cgtggccac 
      aaccccagc caactaact gaagactac gccaacatg 
      tcgctgaac aacacgaag aagcgaccc aatcgcaag 
      aaagacaag aaccgatcg gagccaatc aacatccag 
      ggcgggccc aacccgcgc tcgctcctc ctcgcgcgc 
      aaacactcg accgggact ccgcccacg atgtatctg 
      ccgctcgtc gaggccacg cctcacgac tcgaaggac 
      agttcgagt tcgagtatc aacacccct tccagttcc 
      tcgaccatt tttccgatt agtctgaac agtccgtcg 
      aaggccacg cccgactat ataccgatg gatttcgac 
      aaatccgat tacgtgaat tacgacccg aagccggga 
      gtgggggag tttcggcca atcaatgag agtaacgac 
      gacgagggg cgggcttac gaggttttg aggccgagt 
      tcgagcgag tcgtgttcg acgatcggg ggttctcgg 
      ccggcgtcg gttagttcc gcgcatatg cattacgcg 
      agcttggat ttggatgag ggggggaat cggagtccg 
      aggacggtg aaagggagt gggggggag caaggggaa 
      gtgacgttg acgtatgcc gccattgat tttgtcaaa 
      agtgaaggg ttgaagcac aatgcggtg gcggcgacc 
      aatgcgaaa gttaaacat tga
    mRNA sequence
    • >TC034013 RA
      tccggttta ggagaatca aatggaaca atgaaaagg 
      aagttttgc acggcccta atggagcgc aatatgatt 
      accacatcg aaaataatt aaaactttc caaaaaaat 
      aaacataat tttttcacc ttgtcagtt ttgccaccc 
      cgaaattta aggttggca gtaaataac gaacggcat 
      tttgtcaaa aactaccaa aaaaaacgt taaaatttg 
      tgcaattcc acttgaggt gtgtgcatt ggcgtaatt 
      ttcgtaagt tggttataa aattcctcg ggtcggtgt 
      aaatcgtcg ccaaagtgg caacgtcct cggggtcgt 
      tcctcgtta tcgaatcct ccggagcga cgcatgcgc 
      ttgtcttgt ttacaagga aaagccgct ccgcattgc 
      gtctgcgac atacgtcgt acgatcgtc tgggcgcct 
      cattgccag ccccgacaa agacagtgc cagtggtgg 
      cgttccgtg tccagacag tgccgcttc agacgtcta 
      caacacatg accgacctc gtcaagatg gacgcgagt 
      cagtgttag tgtcggagt gtcgtaaaa tgtgcgttg 
      atgatgtcg ggcagtgag ggtggagtg gtccgcagt 
      ggctacctc aaaaaactc aaaacctcc cgcaagaag 
      ttcttcgtg ctgcgagcc gagacggcg gacgcgtcc 
      gccaggctg gagtactac gactcggag aggaagttc 
      aacaatggg ctgcccccg aagcggagc atcccgctg 
      aagaattgc ttcaatatc aacaaacgg ctggacacc 
      aaacataaa cacgtcatt gcgttgtac acgaaggac 
      gactgcttc tgcgtcgtc ttggacaac gacgacgac 
      ttggacagc tggttgaag gcgcttttg aagctccaa 
      cacggagaa gaagtcgtc gacggggag acacccaag 
      cccacgttc gagcacgtg tggcaagtg accgtactt 
      aatcgaggc ctcggaaat gccggcctt accggaaat 
      taccgcctc tgtcttacc gacaagact ctgagtctg 
      atcaaaaga gactgcgaa aaaccaaca atcgaactg 
      aacctttcg aatattcga agttgcgga aatctccgc 
      gattttttc ttcttggag attggtaga tcaagtacc 
      ttgggagcc ggcgaatta tggatgcaa acggaagac 
      aacaacatt gcccaaaat gtgcattcg accgttttt 
      catgccatg tctaccaac agcagcagc aaggacgaa 
      ttgggcccg aaaagccgt aatcgttcc tcttcggcc 
      accgaatcc tccaaacct aacatgacg cgaaaaatc 
      cacatcgcc cccaaatgt catatattt ctaccacca 
      gatgtgttc cacactagt gacactcag gtcattccc 
      gcgggcagc tccacgcag caccctctc gtgagcggt 
      gttgccagt ttttatcaa catctaatc cagagagga 
      gccaaagct tcagaacgt cgacattca atatcagca 
      ggtagtcta acccattgt ggcgtcatc agccatcag 
      cgcactcaa tcactgcct ttagcgaat cccgcacct 
      ctttccgat cactcgcac tcacgaaaa cgccccaaa 
      tgtatgagc cgggaacgt tgcgattcg atgccatcc 
      cgggcacga accacatcc gaggggaat caccgacac 
      ttgaatcct tatcggccg gcatcgctg tacggtcgt 
      gacatttcg cacagtccg ccattaggc agtccggtg 
      agtcccccg tgttccacc gattcggca ggaagttcg 
      tacagtttg accgacgaa acggacgtt tgtacggaa 
      atggaacca agtttggga cgctatgga cactcattg 
      accccagac gaggcgatt gccgaggag gactgtcct 
      gatagcccc ccctacaat aattacatt tcgatgacg 
      ttgcacagc tcggacgat ggatatgtg gatatgtcg 
      cccagggga cggcatcat aattcgccg acggcgagt 
      atgagtagc gtcacttcg gggactccc tcaacggac 
      atgcgcttc gcggagtat ccccttgag aaggtgacg 
      tcgtacttg gccgacgat gacggacgg ccagctcgg 
      gcatattcg gtcggttcc aaaccggaa cggtataaa 
      aaatacacg gaaatgggc ggaaacagc gataattta 
      cgggcacgt gcgttaagt gttgggtcg aaaaaacca 
      ccagctagg gttttacca ccgcatggg taccaccat 
      cctggtacc aagtcgtca agtgcgccc atcttaacc 
      aattcccgc ggtcagggc tcgtacaat tccatcggc 
      ccaatggac gatctcatg gaaatggat ttcagtcat 
      aactccggt tacatggac atgaagccg gtcaactcc 
      ggttacgtg gaaatgaaa ccgggccgt aaaccggaa 
      acctcacct tacgtcgac atgtcgtcc ggttcttcc 
      ccggccaaa ccgtcgtac atttctccc gccgccacc 
      acgacccaa accgaagtc tacatggag atggaccct 
      cgaaaaaat cacagcgag tatttatcg atgagtttt 
      actaaaaaa ctcccaagt ccggggagt cccgggacc 
      cccgaaggc tacgtcgag atgtcgctc gggcgtggc 
      cacaacccc agccaacta actgaagac tacgccaac 
      atgtcgctg aacaacacg aagaagcga cccaatcgc 
      aagaaagac aagaaccga tcggagcca atcaacatc 
      cagggcggg cccaacccg cgctcgctc ctcctcgcg 
      cgcaaacac tcgaccggg actccgccc acgatgtat 
      ctgccgctc gtcgaggcc acgcctcac gactcgaag 
      gacagttcg agttcgagt atcaacacc ccttccagt 
      tcctcgacc atttttccg attagtctg aacagtccg 
      tcgaaggcc acgcccgac tatataccg atggatttc 
      gacaaatcc gattacgtg aattacgac ccgaagccg 
      ggagtgggg gagtttcgg ccaatcaat gagagtaac 
      gacgacgag gggcgggct tacgaggtt ttgaggccg 
      agttcgagc gagtcgtgt tcgacgatc gggggttct 
      cggccggcg tcggttagt tccgcgcat atgcattac 
      gcgagcttg gatttggat gaggggggg aatcggagt 
      ccgaggacg gtgaaaggg agtgggggg gagcaaggg 
      gaagtgacg ttgacgtat gccgccatt gattttgtc 
      aaaagtgaa gggttgaag cacaatgcg gtggcggcg 
      accaatgcg aaagttaaa cattgaaaa agagactga 
      ttttaggta attgtaggg ttaccatgg acttgtcat 
      atccggttg acttgctta tattattat gtatatgta 
      taagaatta aacaattgt tggatgttt catgtagac 
      gagatagtg aaattttgt tttggtttt gtttgtttg 
      aaacggaaa cgtgtcatg tttggattt tttgcaaat 
      tataggata ttagagtaa tttgtaaac atatcacct 
      ccccttaac gagctagga aatcagagg aggcgattg 
      tggcgacct ctctgtttg tctattcaa tgtatacac 
      aataaatgt tactagatc tctgaagat tcaagaagt 
      gtaaatgaa gatcagcat tccagatca tggggtaca 
      atcattagt gaattattt ccccatccg cgcgactag 
      tgaaaattt ttaaaacta gtatcatca ctaaaaaga 
      tattttata tactactac aatgatggt aaaccaagt 
      tagtgtaat tatatatat ttctatttc acattctta 
      aagcaatgt tatgtaatt gtactatat tatctaaac 
      ctgtttgta attaaaaca agtagttag agaagtgag 
      ttgtaaaca ttattctta ttgaggctc aatttataa 
      ttcctatgt agtcatgaa atgacataa tttctttgt 
      tttttaatt tatataagt tgtatattc taagtgtta 
      atttatttg tatttatat gctttgatt tatcctgaa 
      ataccaaaa catcagaat tgttccact gtaaaactg 
      ccataacgt gaaattagt gaaatcttt atatgcaac 
      aggtgctaa gaatataca cgaacgaac ttttattta 
      tttcgaatg acagtgcct ttacacagt tttttattt 
      gaatttttt aaaattagc attttatct ttgttgctt 
      ttagccata gaacattct gcacttttc gattgattt 
      tatttgttt tttactact tttaaatga ttcaagagc 
      cttgacttt tatcataaa taaaaaaaa ttagaaaat 
      atattaaga aaatgctat ataatcttg aaatagctt 
      atgcaattt atgtagtga tgcatgtaa aaattatta 
      atgtgaaaa ataggaaat ttgctatat gtatagaaa 
      atttggaca ttttcaaat actctgtaa ccaccacca 
      gttcagaat atttctctc attcataaa aaaaggtaa 
      taaacgaat attctggat ggtgactgt ttgaaatca 
      tgtaaagaa atttttagt cctataatt tgcgaaatt 
      tatgtaata ataacgtca acctgtttc gagcactaa 
      gactagtgt ctgtacaca atattggct gcctatatt 
      tttttgtaa atcgacaaa taaattatt tcatttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY