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Gene information
General information
TC004591 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7069189..7081930
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004591
    iBeetle screen iB_10450

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004591 RA
      MMFLQKHCT EFFLLSLFT ATVQCTKLQ EEQVSRWAK 
      KIGDELWKL GEVTTKFKE IQGNYVYLN AQVETKDLE 
      NLISEVGKD IERMMDRKS DAIKCIIEE AENLSQYWV 
      YNNSEYQYY SSKYSNVVN ETKINLAQL SEALKRNEN 
      MYLEMVLND DTHFYNLAV DTSRSSVHV PTNIFDRHE 
      EAAYAIQWS EKLDEIFVR NYNSDPALS WQYFGSTSG 
      IMRHYPAKK WPNIEKDEF DCRVRTWYI EAATCTKDV 
      IILVDNSGS MDGMGRHIA SLTVNTILD TFSNNDYIN 
      ILYYSNQTT NYTIPCFRN LLVQATPEN IVLFKEAIR 
      HLGPSGKTD FPQALQMAF DILENYREI RGCNNEEID 
      EEGKSKACN QAIMLITDG ISRNFSDIV MRNNQLDGG 
      KTIPVRIFT YLIGKEVTN VEEIRWMAC ANRGFYTQV 
      QTLEQVTSA VLQYINVIA RPLVLQGED HPISWTHAY 
      IDMTYDDNK DDQINEPYR LLTSAAVPA FDKKVNQNN 
      ETRVANLLG VAGTDVPIA DIEKLTLPY KLGVNAYAF 
      IVSNNGYIL MHQDFRPMF QKHLRKNYN SIDLVEVEE 
      FDTELPPRH FHPDLKTLR NSIVNGSQG SIHGIPVKY 
      HYDNMRRVT RDKYDYYYV PLNQTPFSL AFAVPDGYG 
      HFSLHVEDE IKNDRHKPG VKITDYFNG SNWKIHPKW 
      VYCKYHYLE GHEYETPEK EILHFLGKV YENDFKWRK 
      QYEAGAGEE LTKVECGRR SHGNDDYYC DKDLVQRLV 
      FDAKNTFGA FQGTSWKHD TDRLFRRFN ATLRFVATM 
      SGLTRWEYI SEEVENNTE REFGDLHAE AIDETWYKS 
      AVIHHRYDP ESFVYSVPF DSGLQEDLL VTASYAVFP 
      TDAGKMAPG CVVGFQFAH KNFMEIVQE ITANVSIEC 
      NKDCGKCGV DLKCYLIDN DGYIIVSEN VQDTGKFFG 
      EIEGDIMGS MLEKKFFEK VIIYDYQAV CNTKDDETS 
      DATVLLTPI NYVRFLANW LIMKISWLA FEINIYNFW 
      APFHVFAQD EKEDGVLIE TKEDDDENE EEEEEEDTY 
      YDCEQERDL FFMLHNNKS YDFPGDISG QGFRHYYIK 
      KVPYSNLIF VAINATMPR TNQQVYSTS PRKIVYSDN 
      ADYPCQKLD LNNLPRRRL AGCYNEHPL ESEIKVCGK 
      TATIKNQFL VTLVMCLFL LQNT
    CDS sequence
    • >TC004591 RA
      atgatgttt ttgcaaaaa cactgtacc gagtttttt 
      ttattatcc ctattcacc gcgactgtt caatgcact 
      aaattacaa gaagaacaa gtctcaaga tgggccaaa 
      aaaatcgga gacgaactg tggaagttg ggcgaagtt 
      acgacaaaa tttaaagaa attcaagga aattatgtt 
      taccttaat gcgcaagtt gaaacgaaa gatttggaa 
      aatttaatt tccgaggtc gggaaagat atcgaacgc 
      atgatggac aggaaaagc gacgctatc aagtgtata 
      atcgaagaa gccgagaat ttatcccaa tattgggtt 
      tacaacaac tcggaatac caatactac agctccaaa 
      tattcaaac gttgtgaat gagactaaa attaacttg 
      gcgcaactg tcggaagcg ctcaaacgg aatgaaaat 
      atgtacctg gagatggta ctcaacgac gatacacac 
      ttttacaat ttggcggtt gacaccagc cgcagttcg 
      gttcacgta ccgacaaat attttcgac agacacgaa 
      gaggctgcg tatgcaatc caatggtcc gaaaaactg 
      gacgaaatt ttcgtacga aactacaac tcagaccct 
      gctctgtcg tggcaatac ttcgggagc acctccggc 
      ataatgagg cattaccca gccaaaaag tggccaaac 
      atcgagaag gacgaattc gactgtcgc gtcaggact 
      tggtacatt gaagcggcc acttgtacc aaagacgtg 
      ataatcctc gtggacaac tctggttca atggatggg 
      atggggcgc cacatcgcc tctctgacc gtgaacaca 
      atccttgac acgttttcg aacaacgac tacatcaac 
      attctttac tacagcaac caaacaacg aactacaca 
      atcccttgc ttccgcaac ttgttggtc caggcgact 
      cctgagaac atcgttctg ttcaaagag gcgatcaga 
      cacttgggg ccttcgggc aaaaccgac tttccgcaa 
      gccctccaa atggcgttc gatattttg gagaactac 
      cgagaaatt cgcggctgt aacaacgaa gaaatcgac 
      gaagagggc aaaagcaag gcttgcaac caagccatt 
      atgttgata actgacgga atttcgcgc aattttagc 
      gacatagtg atgcgaaac aatcagctt gatgggggc 
      aagacaata ccggtgcga attttcact tatttaatc 
      gggaaagag gtcacgaat gttgaggaa attcgatgg 
      atggcgtgc gcaaaccga ggattttac acacaagtc 
      cagactttg gaacaggtg acttcggcc gttttgcaa 
      tatattaac gttattgcc aggcctttg gtgctccag 
      ggtgaagac caccccatt tcgtggact cacgcttat 
      attgacatg acgtatgac gacaacaaa gacgaccaa 
      atcaacgaa ccctatcgc ttattgacc tccgctgcg 
      gtccccgct tttgataaa aaagtcaac caaaacaac 
      gagacacga gttgcaaat ttgttgggg gttgctggc 
      actgatgtg ccaatcgct gatatcgaa aaactgact 
      ttgccgtac aaattgggg gtcaacgct tatgccttt 
      attgtcagt aataatggc tacatttta atgcatcaa 
      gactttcgc cctatgttt caaaagcat ctccgcaaa 
      aactacaac agcattgac ttggtcgaa gtcgaggaa 
      ttcgacact gaactaccc cctcgacat tttcacccc 
      gacttgaaa actttgaga aacagtatt gtgaacggc 
      tcgcaaggg agtatccac ggcattccc gtcaaatat 
      cactatgat aacatgagg agggtcact agagacaaa 
      tacgattat tactacgtg cccttgaac caaacaccc 
      ttttccttg gcgtttgca gtgcctgat gggtacggt 
      catttctcc ctgcatgtg gaggacgaa attaaaaat 
      gaccggcac aagcccgga gtcaaaata accgattat 
      ttcaacgga tccaactgg aaaattcac cccaaatgg 
      gtttactgc aagtaccac tatttggaa ggacacgag 
      tatgaaact ccagagaag gaaattttg cattttttg 
      ggtaaagtg tacgaaaat gacttcaag tggaggaaa 
      cagtacgaa gcgggagcc ggcgaagag ctcaccaaa 
      gtggagtgt ggacgtcgt tcgcacggc aacgacgac 
      tactactgt gacaaagac ctggtgcag aggctggtt 
      tttgacgcg aagaataca ttcggtgcc ttccagggc 
      acctcttgg aagcatgac acggaccgt ttgtttcgc 
      cgtttcaac gccactctc cgatttgtg gccacgatg 
      agtggtctg actcgttgg gagtacatt tctgaggag 
      gtggagaac aacacagag agggagttt ggggacttg 
      cacgcagag gcgattgac gaaacgtgg tacaaaagt 
      gccgtaatc caccatcga tacgacccc gagtcgttt 
      gtatattcc gtgcctttt gattcgggc cttcaggaa 
      gatttgcta gtcactgcc agttatgcg gtttttccc 
      acagatgcg gggaaaatg gccccaggg tgcgtggtg 
      gggttccaa tttgctcac aaaaatttc atggagata 
      gtccaagaa atcaccgcc aatgtttca atcgagtgc 
      aataaggat tgtggaaag tgtggtgtt gatttaaaa 
      tgttaccta atcgacaat gatggctat atcattgtt 
      tccgaaaat gtccaagac acggggaaa ttttttggg 
      gagattgaa ggtgatatc atgggatct atgttggag 
      aaaaaattc tttgaaaaa gttataatt tacgactac 
      caagctgtc tgtaacact aaagatgac gaaaccagt 
      gacgccact gttttacta accccaatt aactacgtc 
      cgttttctg gcaaattgg ctcataatg aaaatatca 
      tggttggct ttcgaaata aacatttat aacttttgg 
      gcacctttt cacgtcttc gcccaggac gaaaaagaa 
      gacggcgtc cttattgaa acgaaagaa gacgatgat 
      gaaaacgaa gaagaagaa gaagaagaa gacacttac 
      tatgactgt gagcaagaa cgtgattta ttttttatg 
      ctacacaac aataaaagt tacgatttt ccgggagat 
      atatctggc caaggtttt aggcattac tacatcaag 
      aaagttccg tacagtaat ctcattttt gtggcaatt 
      aatgcgaca atgccacga accaatcaa caagtttat 
      tccacgtcg cctcgaaaa attgtctac agtgataat 
      gcggattat ccttgccag aaactagac ctcaacaac 
      cttcctcgc agaagatta gccggatgt tataatgaa 
      cacccactg gaaagtgag atcaaagtt tgtgggaaa 
      acggccacg ataaaaaat cagttctta gtgactcta 
      gtgatgtgt ttattttta cttcagaat acatga
    mRNA sequence
    • >TC004591 RA
      atctaaaaa taaagcagt gtattttcg agacccacc 
      gcaataaag taggtctca ttgagtagc aatgaataa 
      aaataaatg atgtttttg caaaaacac tgtaccgag 
      tttttttta ttatcccta ttcaccgcg actgttcaa 
      tgcactaaa ttacaagaa gaacaagtc tcaagatgg 
      gccaaaaaa atcggagac gaactgtgg aagttgggc 
      gaagttacg acaaaattt aaagaaatt caaggaaat 
      tatgtttac cttaatgcg caagttgaa acgaaagat 
      ttggaaaat ttaatttcc gaggtcggg aaagatatc 
      gaacgcatg atggacagg aaaagcgac gctatcaag 
      tgtataatc gaagaagcc gagaattta tcccaatat 
      tgggtttac aacaactcg gaataccaa tactacagc 
      tccaaatat tcaaacgtt gtgaatgag actaaaatt 
      aacttggcg caactgtcg gaagcgctc aaacggaat 
      gaaaatatg tacctggag atggtactc aacgacgat 
      acacacttt tacaatttg gcggttgac accagccgc 
      agttcggtt cacgtaccg acaaatatt ttcgacaga 
      cacgaagag gctgcgtat gcaatccaa tggtccgaa 
      aaactggac gaaattttc gtacgaaac tacaactca 
      gaccctgct ctgtcgtgg caatacttc gggagcacc 
      tccggcata atgaggcat tacccagcc aaaaagtgg 
      ccaaacatc gagaaggac gaattcgac tgtcgcgtc 
      aggacttgg tacattgaa gcggccact tgtaccaaa 
      gacgtgata atcctcgtg gacaactct ggttcaatg 
      gatgggatg gggcgccac atcgcctct ctgaccgtg 
      aacacaatc cttgacacg ttttcgaac aacgactac 
      atcaacatt ctttactac agcaaccaa acaacgaac 
      tacacaatc ccttgcttc cgcaacttg ttggtccag 
      gcgactcct gagaacatc gttctgttc aaagaggcg 
      atcagacac ttggggcct tcgggcaaa accgacttt 
      ccgcaagcc ctccaaatg gcgttcgat attttggag 
      aactaccga gaaattcgc ggctgtaac aacgaagaa 
      atcgacgaa gagggcaaa agcaaggct tgcaaccaa 
      gccattatg ttgataact gacggaatt tcgcgcaat 
      tttagcgac atagtgatg cgaaacaat cagcttgat 
      gggggcaag acaataccg gtgcgaatt ttcacttat 
      ttaatcggg aaagaggtc acgaatgtt gaggaaatt 
      cgatggatg gcgtgcgca aaccgagga ttttacaca 
      caagtccag actttggaa caggtgact tcggccgtt 
      ttgcaatat attaacgtt attgccagg cctttggtg 
      ctccagggt gaagaccac cccatttcg tggactcac 
      gcttatatt gacatgacg tatgacgac aacaaagac 
      gaccaaatc aacgaaccc tatcgctta ttgacctcc 
      gctgcggtc cccgctttt gataaaaaa gtcaaccaa 
      aacaacgag acacgagtt gcaaatttg ttgggggtt 
      gctggcact gatgtgcca atcgctgat atcgaaaaa 
      ctgactttg ccgtacaaa ttgggggtc aacgcttat 
      gcctttatt gtcagtaat aatggctac attttaatg 
      catcaagac tttcgccct atgtttcaa aagcatctc 
      cgcaaaaac tacaacagc attgacttg gtcgaagtc 
      gaggaattc gacactgaa ctaccccct cgacatttt 
      caccccgac ttgaaaact ttgagaaac agtattgtg 
      aacggctcg caagggagt atccacggc attcccgtc 
      aaatatcac tatgataac atgaggagg gtcactaga 
      gacaaatac gattattac tacgtgccc ttgaaccaa 
      acacccttt tccttggcg tttgcagtg cctgatggg 
      tacggtcat ttctccctg catgtggag gacgaaatt 
      aaaaatgac cggcacaag cccggagtc aaaataacc 
      gattatttc aacggatcc aactggaaa attcacccc 
      aaatgggtt tactgcaag taccactat ttggaagga 
      cacgagtat gaaactcca gagaaggaa attttgcat 
      tttttgggt aaagtgtac gaaaatgac ttcaagtgg 
      aggaaacag tacgaagcg ggagccggc gaagagctc 
      accaaagtg gagtgtgga cgtcgttcg cacggcaac 
      gacgactac tactgtgac aaagacctg gtgcagagg 
      ctggttttt gacgcgaag aatacattc ggtgccttc 
      cagggcacc tcttggaag catgacacg gaccgtttg 
      tttcgccgt ttcaacgcc actctccga tttgtggcc 
      acgatgagt ggtctgact cgttgggag tacatttct 
      gaggaggtg gagaacaac acagagagg gagtttggg 
      gacttgcac gcagaggcg attgacgaa acgtggtac 
      aaaagtgcc gtaatccac catcgatac gaccccgag 
      tcgtttgta tattccgtg ccttttgat tcgggcctt 
      caggaagat ttgctagtc actgccagt tatgcggtt 
      tttcccaca gatgcgggg aaaatggcc ccagggtgc 
      gtggtgggg ttccaattt gctcacaaa aatttcatg 
      gagatagtc caagaaatc accgccaat gtttcaatc 
      gagtgcaat aaggattgt ggaaagtgt ggtgttgat 
      ttaaaatgt tacctaatc gacaatgat ggctatatc 
      attgtttcc gaaaatgtc caagacacg gggaaattt 
      tttggggag attgaaggt gatatcatg ggatctatg 
      ttggagaaa aaattcttt gaaaaagtt ataatttac 
      gactaccaa gctgtctgt aacactaaa gatgacgaa 
      accagtgac gccactgtt ttactaacc ccaattaac 
      tacgtccgt tttctggca aattggctc ataatgaaa 
      atatcatgg ttggctttc gaaataaac atttataac 
      ttttgggca ccttttcac gtcttcgcc caggacgaa 
      aaagaagac ggcgtcctt attgaaacg aaagaagac 
      gatgatgaa aacgaagaa gaagaagaa gaagaagac 
      acttactat gactgtgag caagaacgt gatttattt 
      tttatgcta cacaacaat aaaagttac gattttccg 
      ggagatata tctggccaa ggttttagg cattactac 
      atcaagaaa gttccgtac agtaatctc atttttgtg 
      gcaattaat gcgacaatg ccacgaacc aatcaacaa 
      gtttattcc acgtcgcct cgaaaaatt gtctacagt 
      gataatgcg gattatcct tgccagaaa ctagacctc 
      aacaacctt cctcgcaga agattagcc ggatgttat 
      aatgaacac ccactggaa agtgagatc aaagtttgt 
      gggaaaacg gccacgata aaaaatcag ttcttagtg 
      actctagtg atgtgttta tttttactt cagaataca 
      tgattgtgt atataataa atgtctatt ttctgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY