Status
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Gene information
General information
TC006259 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5917957..5922535
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006259
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10449

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006259 RA
      MEFMKIFDE KFHEIVKSG IRSILLENE PEDVENSIL 
      KDWLPEKPN SLACSYCRV EFDQVCQQR EHYKLDWHR 
      YNLRQSLLQ KPPITEEEF DEKTTHDDI SSISGSDSE 
      KDDNLDSFA TAQGKIFLQ NEQGTVFSI YACLLYRKK 
      EELQNQVLK TRLKECCVS NRKWAVLML GGGHFAGAV 
      FDNDQVVVH KTFHCYTVR AGQGGAQSS RDAKSGGSG 
      PKSAGASLR RYNEQALVQ HVRGIVDTW KSQLDECAL 
      ILYRASGPY NRGVLFGGG DPVFDKNDA RLRTIPFST 
      RRATFTEVK RVHSEMTTA QVYHSYDSA IQYFTRRKN 
      TKRVKTRSP CIDRAKSRE IIERPLPVT HETSSDEEV 
      LNLSTENNE ISFVDSLQE FEDTLTPEQ RRKRKPKKS 
      KTKQNEQRK HELIQTLQK GDIETLARV VNDFNEIVD 
      DDGNTLLHI AAMNEQNAT LSFLLEKGA NPCAKNHKQ 
      QTPYTCTQS KEIRESLKQ FARDFPDKY NYNKAQIPT 
      NVLTPEELA EKKKAQRKL KKEKEKIKK KENEIKRRE 
      EMEKERFLK LSDREKRAL AAERRILSQ SGTVITRCF 
      LCACDMTGK VPFEYLGNR FCSVECLKA HRLQNPQIL
    CDS sequence
    • >TC006259 RA
      atggagttt atgaaaatt tttgatgaa aagttccac 
      gaaattgtc aaatctgga attagaagc attttactg 
      gaaaacgag cctgaagat gtagaaaat tcgattttg 
      aaagactgg cttccggaa aaaccaaat tccctagct 
      tgctcctac tgtcgggta gaattcgat caggtgtgc 
      caacagcgc gagcattac aaactcgat tggcaccgt 
      tacaacctg cgccagtcc cttcttcaa aaaccgcca 
      ataaccgaa gaagaattc gacgagaaa accactcac 
      gacgatatt tccagcatt tcgggctca gactccgaa 
      aaagacgac aatttggac agttttgcc acagctcaa 
      ggcaagatt ttcctccaa aatgaacaa ggaactgtg 
      ttttcaatt tacgcgtgt cttctctat cggaaaaaa 
      gaggaatta caaaaccaa gtattaaaa acccggctg 
      aaagaatgt tgtgtgagt aacaggaag tgggctgtt 
      ttgatgctg ggagggggc cacttcgcg ggggctgtc 
      ttcgataac gaccaagtt gtggtgcac aaaaccttc 
      cattgttac actgtgagg gccggtcaa gggggggca 
      cagtcctca cgtgatgct aaaagtggg ggctcgggg 
      cccaaaagt gcaggggcg tctttgaga cgatacaac 
      gaacaagcg ttagtacaa cacgtgaga gggattgtg 
      gacacgtgg aagtcgcag ttggacgaa tgcgcgctt 
      attttatac agggcttcg gggccctac aaccggggg 
      gtgcttttc gggggtggc gaccccgtg tttgataaa 
      aatgatgct agattaagg actattccg ttttcgaca 
      agacgggcc acttttacg gaagttaag agagttcat 
      tcggagatg acaacggct caagtgtat cactcgtat 
      gactcggct attcaatat ttcacacgt cggaaaaac 
      actaaacgg gtcaaaacg agatcacca tgcattgac 
      agggccaaa tcaagagaa ataatcgag cgccctctg 
      cccgtaaca catgaaaca agttccgac gaagaagtc 
      ttaaaccta tcgactgaa aacaacgaa atttcattc 
      gtcgattcg ctccaggag ttcgaggat acgctaaca 
      cccgaacaa agacggaaa cgcaaaccg aaaaaaagt 
      aaaacaaaa caaaacgag caacgaaaa cacgaatta 
      atccaaacg ttgcaaaaa ggcgatatt gagactttg 
      gcccgcgtt gtgaacgac tttaatgaa attgtcgac 
      gatgacggg aatacttta ctccacatc gctgcaatg 
      aacgaacaa aacgcaact cttagtttc ctactggaa 
      aaaggggcc aatccttgc gccaaaaac cacaaacag 
      caaacgccc tacacttgc acacaaagt aaggaaatt 
      cgtgagagt ttgaaacaa ttcgctcgg gatttcccc 
      gataaatac aactacaac aaggcgcaa attccgacc 
      aatgtcctc acaccggag gaattagcc gagaagaag 
      aaagcgcaa cgaaaactc aaaaaggaa aaggagaaa 
      atcaaaaag aaggaaaat gagattaaa cgacgcgag 
      gaaatggaa aaggagagg tttttgaaa ttgagcgac 
      cgcgaaaaa cgggcattg gccgccgaa cgacggatt 
      ttaagccag tctgggact gtgataact cggtgtttc 
      ctatgcgct tgtgacatg accgggaaa gtgccgttc 
      gagtatttg ggaaatcgg ttttgttca gtggagtgt 
      ctcaaagcc caccggtta caaaatcca caaatcttg 
      tag
    mRNA sequence
    • >TC006259 RA
      gttatttta ctaataatt gtaatcgtg tcgtgatgg 
      tcgtgtctc tgatttagt ccacgatgg agtttatga 
      aaatttttg atgaaaagt tccacgaaa ttgtcaaat 
      ctggaatta gaagcattt tactggaaa acgagcctg 
      aagatgtag aaaattcga ttttgaaag actggcttc 
      cggaaaaac caaattccc tagcttgct cctactgtc 
      gggtagaat tcgatcagg tgtgccaac agcgcgagc 
      attacaaac tcgattggc accgttaca acctgcgcc 
      agtcccttc ttcaaaaac cgccaataa ccgaagaag 
      aattcgacg agaaaacca ctcacgacg atatttcca 
      gcatttcgg gctcagact ccgaaaaag acgacaatt 
      tggacagtt ttgccacag ctcaaggca agattttcc 
      tccaaaatg aacaaggaa ctgtgtttt caatttacg 
      cgtgtcttc tctatcgga aaaaagagg aattacaaa 
      accaagtat taaaaaccc ggctgaaag aatgttgtg 
      tgagtaaca ggaagtggg ctgttttga tgctgggag 
      ggggccact tcgcggggg ctgtcttcg ataacgacc 
      aagttgtgg tgcacaaaa ccttccatt gttacactg 
      tgagggccg gtcaagggg gggcacagt cctcacgtg 
      atgctaaaa gtgggggct cggggccca aaagtgcag 
      gggcgtctt tgagacgat acaacgaac aagcgttag 
      tacaacacg tgagaggga ttgtggaca cgtggaagt 
      cgcagttgg acgaatgcg cgcttattt tatacaggg 
      cttcggggc cctacaacc ggggggtgc ttttcgggg 
      gtggcgacc ccgtgtttg ataaaaatg atgctagat 
      taaggacta ttccgtttt cgacaagac gggccactt 
      ttacggaag ttaagagag ttcattcgg agatgacaa 
      cggctcaag tgtatcact cgtatgact cggctattc 
      aatatttca cacgtcgga aaaacacta aacgggtca 
      aaacgagat caccatgca ttgacaggg ccaaatcaa 
      gagaaataa tcgagcgcc ctctgcccg taacacatg 
      aaacaagtt ccgacgaag aagtcttaa acctatcga 
      ctgaaaaca acgaaattt cattcgtcg attcgctcc 
      aggagttcg aggatacgc taacacccg aacaaagac 
      ggaaacgca aaccgaaaa aaagtaaaa caaaacaaa 
      acgagcaac gaaaacacg aattaatcc aaacgttgc 
      aaaaaggcg atattgaga ctttggccc gcgttgtga 
      acgacttta atgaaattg tcgacgatg acgggaata 
      ctttactcc acatcgctg caatgaacg aacaaaacg 
      caactctta gtttcctac tggaaaaag gggccaatc 
      cttgcgcca aaaaccaca aacagcaaa cgccctaca 
      cttgcacac aaagtaagg aaattcgtg agagtttga 
      aacaattcg ctcgggatt tccccgata aatacaact 
      acaacaagg cgcaaattc cgaccaatg tcctcacac 
      cggaggaat tagccgaga agaagaaag cgcaacgaa 
      aactcaaaa aggaaaagg agaaaatca aaaagaagg 
      aaaatgaga ttaaacgac gcgaggaaa tggaaaagg 
      agaggtttt tgaaattga gcgaccgcg aaaaacggg 
      cattggccg ccgaacgac ggattttaa gccagtctg 
      ggactgtga taactcggt gtttcctat gcgcttgtg 
      acatgaccg ggaaagtgc cgttcgagt atttgggaa 
      atcggtttt gttcagtgg agtgtctca aagcccacc 
      ggttacaaa atccacaaa tcttgtagg agtttatta 
      ctggaaaaa taaccataa aatcaacta aataaatgc 
      atcactaga ttgtccatc aaggaagtc gtcaaacaa 
      tttctctaa aaatatcta acaataatt cacagattt 
      ggtcctttg gtagaaaat tacaaaatt gtatttctc 
      atactgaac gaaaataaa tacttgaaa aaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY