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Gene information
General information
TC034009
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030663
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5828308..5832265
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034009
    iBeetle screen iB_10448

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034009 RA
      MSLEPTGDI LDQYHTISS KLKKRFLRK PNVTEACES 
      FSSLGKQCE ASELPAYAG MCWISSARC EGSLGNTPG 
      EASCLTRAA RQFLSAETK DHELGCPSP CQENLEAAV 
      GCFAYAASR YPEDCPIPV GLNLELVDF LKKVDRECD 
      VEKHLQNTV ELSKDNLDT KIFCLELLA SHYIESRDY 
      TGALNTFND IASIVETLP LNGARAETM LKCEVSRVL 
      LLLILRPAP QKLSPNLAK LLEKYTWGD QSDKALKAC 
      GMSQRMFIL LQSLVAACQ SLDTSNLDH LESELWKMV 
      GKEERELLR ILLFVMDFQ TRETHSSRL WYYFWQFLY 
      LILIAGILH YVQMVFWSR DLRYSPQTY LDVVDMPQI 
      HCKKLLYEV SHLHKEIIE MKEQLEELR SWKVNYDEN 
      SKTNLKKAL DNFYSDTIG IFDYASESA GGSVISTPE 
      TTPLPSPFG LRFFGTIDW VGQSDPGVL LQPNTLPGR 
      CYAFYGNKG RIRVKLGRP IMVTHVSLE HIRIGESTT 
      RAPKNFEIW GFGERGEEN LGQFVYDLD DSIIQTFGV 
      IAKNRTFRA VELRILSNH GNAESTCVY RFRVHSLD
    CDS sequence
    • >TC034009 RA
      atgagtctt gaaccaact ggcgatatt ttggaccag 
      tatcacacg atttcgagc aaactgaaa aagcgcttc 
      ctgcgtaag ccaaacgtt acagaagcc tgcgagtcg 
      ttcagttcg ttggggaaa caatgcgaa gcctcagaa 
      ctgccagct tatgccgga atgtgctgg atttcgtca 
      gcaaggtgc gaggggtct ttggggaac accccaggt 
      gaggcgtct tgtttgacg cgagccgcg cgccagttt 
      ttatcagct gagacgaaa gaccacgag ttggggtgc 
      cccagccct tgtcaggag aacctggag gctgctgtg 
      ggttgtttt gcttatgcg gcgagtcgc tacccggaa 
      gactgccct attcccgtg ggactcaac ctcgaattg 
      gttgatttt ttgaaaaaa gttgacagg gagtgcgat 
      gttgaaaag catttacaa aatacagtt gaattatca 
      aaggataat ttagacact aaaattttt tgtttggaa 
      ttgctagcg tcccattat atagaaagt cgcgactac 
      acaggagcg ctaaacacc tttaatgat attgcttcg 
      attgttgaa acgttgcct ctgaatggg gcacgagct 
      gagaccatg ttgaaatgt gaagttagt cgagtcttg 
      ctcttgttg attttgagg cctgcgccc caaaaactg 
      tcgccgaat ttggccaag ttactcgaa aagtacaca 
      tggggggac caaagtgat aaagcttta aaagcttgt 
      gggatgtct caaaggatg tttatttta ctgcagtcg 
      cttgttgcc gcttgtcag tcgttagat acttcgaat 
      cttgatcat ttagaaagc gagttgtgg aaaatggtt 
      gggaaagaa gaacgggaa ttgcttaga attttgtta 
      tttgtaatg gattttcag acacgcgaa acgcattcc 
      tcacgtttg tggtactat ttctggcaa tttttgtac 
      ttaattctg attgctggc attcttcac tacgtccaa 
      atggtattt tggagtcgc gatttgcgg tacagcccc 
      caaacttac ctggacgtc gtcgacatg ccccaaatc 
      cactgcaaa aaattactt tacgaagtg agccatctt 
      cacaaggaa ataattgaa atgaaagaa cagttggaa 
      gaacttcgt tcatggaaa gtcaactac gacgaaaat 
      tccaaaacc aacttgaaa aaagcgttg gataatttc 
      tactcagac acaatcgga atttttgac tatgcctcc 
      gagtccgcc gggggctcc gtcatctcc acccctgaa 
      accaccccc ctgccgtcg cccttcggc ctccgcttt 
      ttcggcacc atcgactgg gtgggccag tccgaccct 
      ggagttttg ctccagccg aacacgctt ccagggcgg 
      tgttacgcc ttttacggg aacaagggt cgtattcgc 
      gtaaaattg gggcgcccg attatggta acccacgtg 
      agtttggag cacatccgc attggggag agcacaacg 
      agggccccc aaaaatttc gaaatttgg ggtttcggg 
      gagaggggt gaggagaat ttaggtcag ttcgtgtac 
      gatttggac gattcgatt attcaaact tttggcgta 
      attgcgaaa aatcggacg tttcgagcc gtggaattg 
      cgcatcctc agtaatcac ggcaacgct gaatcgacg 
      tgtgtgtac cgattcagg gttcattct ttggattag
    mRNA sequence
    • >TC034009 RA
      gtcgcactt ttgaccttc tcgattttc tcagatatt 
      tttctgagc gaattaagc tccggaaac tcaaaattt 
      ggttcactc tctttccgt tgatttccc tttttccct 
      acattttcg cgtttagcg tcagtcaca agtttggtt 
      ttgggcacc ggcagctcg gtattccgc acaagagtc 
      accttggtt tcgtttttc ttaaattta ctcatgatt 
      gtttacccc taattcaaa tttgtgagg ttatgtctg 
      cccaaaagt gaggttata caagatgag tcttgaacc 
      aactggcga tattttgga ccagtatca cacgatttc 
      gagcaaact gaaaaagcg cttcctgcg taagccaaa 
      cgttacaga agcctgcga gtcgttcag ttcgttggg 
      gaaacaatg cgaagcctc agaactgcc agcttatgc 
      cggaatgtg ctggatttc gtcagcaag gtgcgaggg 
      gtctttggg gaacacccc aggtgaggc gtcttgttt 
      gacgcgagc cgcgcgcca gtttttatc agctgagac 
      gaaagacca cgagttggg gtgccccag cccttgtca 
      ggagaacct ggaggctgc tgtgggttg ttttgctta 
      tgcggcgag tcgctaccc ggaagactg ccctattcc 
      cgtgggact caacctcga attggttga ttttttgaa 
      aaaagttga cagggagtg cgatgttga aaagcattt 
      acaaaatac agttgaatt atcaaagga taatttaga 
      cactaaaat tttttgttt ggaattgct agcgtccca 
      ttatataga aagtcgcga ctacacagg agcgctaaa 
      cacctttaa tgatattgc ttcgattgt tgaaacgtt 
      gcctctgaa tggggcacg agctgagac catgttgaa 
      atgtgaagt tagtcgagt cttgctctt gttgatttt 
      gaggcctgc gccccaaaa actgtcgcc gaatttggc 
      caagttact cgaaaagta cacatgggg ggaccaaag 
      tgataaagc tttaaaagc ttgtgggat gtctcaaag 
      gatgtttat tttactgca gtcgcttgt tgccgcttg 
      tcagtcgtt agatacttc gaatcttga tcatttaga 
      aagcgagtt gtggaaaat ggttgggaa agaagaacg 
      ggaattgct tagaatttt gttatttgt aatggattt 
      tcagacacg cgaaacgca ttcctcacg tttgtggta 
      ctatttctg gcaattttt gtacttaat tctgattgc 
      tggcattct tcactacgt ccaaatggt attttggag 
      tcgcgattt gcggtacag cccccaaac ttacctgga 
      cgtcgtcga catgcccca aatccactg caaaaaatt 
      actttacga agtgagcca tcttcacaa ggaaataat 
      tgaaatgaa agaacagtt ggaagaact tcgttcatg 
      gaaagtcaa ctacgacga aaattccaa aaccaactt 
      gaaaaaagc gttggataa tttctactc agacacaat 
      cggaatttt tgactatgc ctccgagtc cgccggggg 
      ctccgtcat ctccacccc tgaaaccac ccccctgcc 
      gtcgccctt cggcctccg ctttttcgg caccatcga 
      ctgggtggg ccagtccga ccctggagt tttgctcca 
      gccgaacac gcttccagg gcggtgtta cgcctttta 
      cgggaacaa gggtcgtat tcgcgtaaa attggggcg 
      cccgattat ggtaaccca cgtgagttt ggagcacat 
      ccgcattgg ggagagcac aacgagggc ccccaaaaa 
      tttcgaaat ttggggttt cggggagag gggtgagga 
      gaatttagg tcagttcgt gtacgattt ggacgattc 
      gattattca aacttttgg cgtaattgc gaaaaatcg 
      gacgtttcg agccgtgga attgcgcat cctcagtaa 
      tcacggcaa cgctgaatc gacgtgtgt gtaccgatt 
      cagggttca ttctttgga ttagttggc attgaacat 
      tcacttgtt acgtcatcc gccattatt atctctggt 
      gtgaagtta gccgcggag agttcgacc ttaagtctg 
      gtttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY