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Gene information
General information
TC011191 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19286526..19295665
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011191
    iBeetle screen iB_10445

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    previtellogenic egg chamber sometimes not present - Number of animals showing the phenotype: 2

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011191 RA
      MYEPVQIDM SNYPSILTP PSSSEDSPQ TVFYNSPTA 
      CLPMLPQTS QAFDFDGVT HPPPPFLRI IEQPIDRFR 
      FRYKSEMTG THGSLCGQN SDRSRKQTY PTIELCNFY 
      KRAVVRCSI YQYNTKEDQ KPHPHRLIK KQGKGEVDD 
      PHDAYVSPE DGYVYTFHS MGIIHTAKK NLVPELVKK 
      KTQLKKEEV ARLEGKMRE LTQKEEIEI KGLAEAESK 
      FINLNVVVL RFDAFYENN GILHPICPP VYSHTINNL 
      KSALTGELK IVRMDHCTS PAKGGREVF ILVERVTKK 
      NIKIRLYEL DDDDNILWE DYGRFSDLD VHHQYAIVF 
      KTPPYRDPN IKSPVKVYI ELERPSDRS RSEAKEFTY 
      TPSSSVYKP GAKRARTSY DTSYDSSLP SDELPLPIN 
      NLCIDNTPG LLDDVQLEV TSDDLQQAM TNIDSEEFR 
      RLFNEFGQD YSLVVDAPS QYPKKNAPK GGNKNRSMK 
      SSKSNYQRA DNSEDIVMA KQAVMELQN FIKTKPSDE 
      KAISMLRHY LMTEDKTNA LHVSIKQNK QADVIFLLK 
      MVYVWKQYH LTNIYNDQC ETALHIATC MHLEEIVSN 
      LIVCKADAS IGDSDMNTA LHLAVITNA PTPIFEKLL 
      SGDHVKKFI DNENNEGNT ALNLAIENS NMRALKLLL 
      IHGADINKQ HKKNGYTPL RFAIEKQNL DLVRYILQH 
      EKLNPMIED FKDINPLQA AMKKDNSEL IDLITEFMR 
      KNNYVDVEI KNEIEEYSD EEIEEKYSV KLEQISTED 
      LHQMYAKVT NFTPECLEE ISRILDESQ TWQNLADLL 
      DLGHLTRSG LCDSKNSPT KLLLKVAIE TNGDSVLQI 
      RDFLENLDE TRAVEIIDN MVRTQFC
    CDS sequence
    • >TC011191 RA
      atgtacgag cctgtgcag attgatatg agcaattat 
      ccgagcatt ttgaccccg ccgtcgagt agcgaagat 
      tcgccccag acggttttt tacaactcg cccacggca 
      tgtttaccg atgttgccc cagacgtcg caagcgttc 
      gatttcgac ggggtcacg cacccaccg ccccctttc 
      ttgcggatt attgagcaa cctatcgat aggtttagg 
      tttaggtat aagtctgaa atgaccgga acacacggg 
      agtttgtgt gggcagaac tccgacagg agtcggaaa 
      caaacgtac cccactatt gaactgtgt aatttttat 
      aaacgagca gtggttcgg tgctccatt taccaatac 
      aacactaaa gaagaccaa aagccgcat ccacacaga 
      ctaattaaa aaacaaggg aaaggagaa gttgatgat 
      ccacacgat gcttacgtc tcacctgaa gatggttac 
      gtttatacg ttccatagt atgggtatt atacacaca 
      gctaaaaaa aatctagtg ccagaatta gtcaagaaa 
      aagacacaa ttgaagaag gaagaagtc gcaaggctt 
      gaagggaaa atgcgcgag ttgactcaa aaggaagaa 
      attgagatt aagggattg gctgaggct gaaagcaaa 
      ttcattaat ctgaatgtt gttgtcttg cgatttgac 
      gctttttat gaaaacaat gggatacta catcctatt 
      tgcccgccg gtttattca cacacgatc aataacttg 
      aaaagcgcc ctcacgggc gaattaaaa atcgtgcga 
      atggaccac tgcaccagc cctgcgaaa gggggccga 
      gaggtgttt attttagtc gagagagtc accaaaaag 
      aacattaaa atacgcttg tatgagttg gacgacgat 
      gacaacatc ttgtgggag gattatggc cgtttcagc 
      gacttggac gtccaccac cagtacgcc attgtcttc 
      aaaacgccc ccatacaga gaccccaac atcaaatcc 
      ccggttaag gtttatatt gagctcgaa cggccgtcc 
      gatcgctcc cgaagtgag gcgaaagag ttcacttac 
      acccccagt tcaagtgtt tataagccg ggtgccaaa 
      agagcaagg acgagttat gacactagt tacgattcg 
      tctttacct agtgatgaa ttaccgcta ccgatcaat 
      aatttatgt atcgataac acgccaggt ttgcttgat 
      gatgttcag ttagaagtt accagtgat gacctacaa 
      caagcgatg actaacatt gattcggag gagtttcgt 
      cgattgttc aatgagttt ggacaagac tattcattg 
      gtggtggat gcaccctcg cagtatccc aaaaaaaat 
      gcccccaag gggggtaac aagaataga tcgatgaaa 
      tcgtcaaaa tcaaactac caaagagcg gacaacagc 
      gaggacata gtaatggcc aaacaggca gtcatggag 
      ttacaaaac ttcattaaa acaaaacct agtgacgaa 
      aaagccatc agtatgttg cgccattac ttaatgact 
      gaagataaa accaatgcc ttgcatgtt tccatcaag 
      cagaataag caagccgac gtgatattt ttactcaaa 
      atggtgtac gtttggaaa cagtaccat ttaaccaac 
      atttacaac gaccagtgc gagaccgct cttcacatc 
      gccacttgt atgcatttg gaggaaatt gtttcgaac 
      ttaatcgtg tgtaaagcg gacgcttca atcggtgat 
      tccgacatg aacactgct ctacatctc gctgtcata 
      actaacgca cccacgccc atttttgag aagttacta 
      tcgggcgat cacgtcaaa aaattcata gataatgaa 
      aataatgag ggcaacact gcgcttaat ttggcgatc 
      gagaattcc aacatgcgg gccttaaaa ttactgttg 
      attcacggt gctgatatt aataagcaa cacaaaaag 
      aacggttat acacctctc aggtttgcc atcgagaaa 
      caaaatctt gatttagtt cgatatatt ttgcaacac 
      gagaagctc aatccgatg attgaggat ttcaaggac 
      attaacccg ttacaggcg gccatgaaa aaggataat 
      tcggaactg attgatcta attaccgaa tttatgcgc 
      aaaaataac tacgttgat gtggaaatt aagaacgaa 
      atcgaggaa tattccgac gaggaaatt gaggagaaa 
      tactcggtg aaattggag caaatttcc accgaagac 
      ttgcaccaa atgtacgct aaagtaaca aatttcacc 
      cccgagtgt ttggaggag atctcgcgc attttagat 
      gagtcccaa acgtggcag aatttggcc gatttgctc 
      gatttgggg catttgaca cgttcagga ctctgtgat 
      tcgaaaaac agccccacg aagttgtta ctaaaggtg 
      gcgattgag accaatggc gacagtgtc ttgcaaatt 
      agggacttc ctggagaat ttggacgag acgcgggcg 
      gttgaaatt attgacaat atggtcagg acgcagttt 
      tgttag
    mRNA sequence
    • >TC011191 RA
      ggggatttt ttggcgctc gtccttccc cgtacaccc 
      ccgcgcacg ccattatcc ggacgtggg gaataacct 
      atttctgtg gtaatgacg caatagttc gaggtgagc 
      ataaacatg gtttattat tgttcgaaa cgggcccca 
      ctttgcaca gagtcgtcg gtatcacac atcggcgaa 
      ctttcgaaa gtttgtgtc caaagtgta ggttaggat 
      gctaaccga aagacccaa aatgtacga gcctgtgca 
      gattgatat gagcaatta tccgagcat tttgacccc 
      gccgtcgag tagcgaaga ttcgcccca gacggtttt 
      ttacaactc gcccacggc atgtttacc gatgttgcc 
      ccagacgtc gcaagcgtt cgatttcga cggggtcac 
      gcacccacc gcccccttt cttgcggat tattgagca 
      acctatcga taggtttag gtttaggta taagtctga 
      aatgaccgg aacacacgg gagtttgtg tgggcagaa 
      ctccgacag gagtcggaa acaaacgta ccccactat 
      tgaactgtg taattttta taaacgagc agtggttcg 
      gtgctccat ttaccaata caacactaa agaagacca 
      aaagccgca tccacacag actaattaa aaaacaagg 
      gaaaggaga agttgatga tccacacga tgcttacgt 
      ctcacctga agatggtta cgtttatac gttccatag 
      tatgggtat tatacacac agctaaaaa aaatctagt 
      gccagaatt agtcaagaa aaagacaca attgaagaa 
      ggaagaagt cgcaaggct tgaagggaa aatgcgcga 
      gttgactca aaaggaaga aattgagat taagggatt 
      ggctgaggc tgaaagcaa attcattaa tctgaatgt 
      tgttgtctt gcgatttga cgcttttta tgaaaacaa 
      tgggatact acatcctat ttgcccgcc ggtttattc 
      acacacgat caataactt gaaaagcgc cctcacggg 
      cgaattaaa aatcgtgcg aatggacca ctgcaccag 
      ccctgcgaa agggggccg agaggtgtt tattttagt 
      cgagagagt caccaaaaa gaacattaa aatacgctt 
      gtatgagtt ggacgacga tgacaacat cttgtggga 
      ggattatgg ccgtttcag cgacttgga cgtccacca 
      ccagtacgc cattgtctt caaaacgcc cccatacag 
      agaccccaa catcaaatc cccggttaa ggtttatat 
      tgagctcga acggccgtc cgatcgctc ccgaagtga 
      ggcgaaaga gttcactta cacccccag ttcaagtgt 
      ttataagcc gggtgccaa aagagcaag gacgagtta 
      tgacactag ttacgattc gtctttacc tagtgatga 
      attaccgct accgatcaa taatttatg tatcgataa 
      cacgccagg tttgcttga tgatgttca gttagaagt 
      taccagtga tgacctaca acaagcgat gactaacat 
      tgattcgga ggagtttcg tcgattgtt caatgagtt 
      tggacaaga ctattcatt ggtggtgga tgcaccctc 
      gcagtatcc caaaaaaaa tgcccccaa ggggggtaa 
      caagaatag atcgatgaa atcgtcaaa atcaaacta 
      ccaaagagc ggacaacag cgaggacat agtaatggc 
      caaacaggc agtcatgga gttacaaaa cttcattaa 
      aacaaaacc tagtgacga aaaagccat cagtatgtt 
      gcgccatta cttaatgac tgaagataa aaccaatgc 
      cttgcatgt ttccatcaa gcagaataa gcaagccga 
      cgtgatatt tttactcaa aatggtgta cgtttggaa 
      acagtacca tttaaccaa catttacaa cgaccagtg 
      cgagaccgc tcttcacat cgccacttg tatgcattt 
      ggaggaaat tgtttcgaa cttaatcgt gtgtaaagc 
      ggacgcttc aatcggtga ttccgacat gaacactgc 
      tctacatct cgctgtcat aactaacgc acccacgcc 
      catttttga gaagttact atcgggcga tcacgtcaa 
      aaaattcat agataatga aaataatga gggcaacac 
      tgcgcttaa tttggcgat cgagaattc caacatgcg 
      ggccttaaa attactgtt gattcacgg tgctgatat 
      taataagca acacaaaaa gaacggtta tacacctct 
      caggtttgc catcgagaa acaaaatct tgatttagt 
      tcgatatat tttgcaaca cgagaagct caatccgat 
      gattgagga tttcaagga cattaaccc gttacaggc 
      ggccatgaa aaaggataa ttcggaact gattgatct 
      aattaccga atttatgcg caaaaataa ctacgttga 
      tgtggaaat taagaacga aatcgagga atattccga 
      cgaggaaat tgaggagaa atactcggt gaaattgga 
      gcaaatttc caccgaaga cttgcacca aatgtacgc 
      taaagtaac aaatttcac ccccgagtg tttggagga 
      gatctcgcg cattttaga tgagtccca aacgtggca 
      gaatttggc cgatttgct cgatttggg gcatttgac 
      acgttcagg actctgtga ttcgaaaaa cagccccac 
      gaagttgtt actaaaggt ggcgattga gaccaatgg 
      cgacagtgt cttgcaaat tagggactt cctggagaa 
      tttggacga gacgcgggc ggttgaaat tattgacaa 
      tatggtcag gacgcagtt ttgttagtt tctactatt 
      agtttagtt tgatcatgt ctttgtatt aatttcgtt 
      catttttat gttagagaa atttgtaat aaaggtatt 
      gttccaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY