Status
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Gene information
General information
TC033977
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005992
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 4114093..4116076
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033977
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10436

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033977 RA
      MDPTNRCEV CEKPALHKC GKCHNAHYC DKKHQREHW 
      KQHKSVCQP FKICEDAVL GRHLVATRA LKPGEVVIQ 
      EAPLIWGPS YNSIPVCLG CGKAVDETC SRPCQKCGW 
      PVCSDLCEK SPSHIPECR YTSQRGEKI TVKIFGVPH 
      PIYKCITVL RCLYQKQFL PKNWKKLET LQNHLEEHK 
      STTNYESDR VTIAEFIRR FFKLSATFS EEDIMKVHG 
      STLVNGHEV PLTEPFHVA IYSSASMLE HSCGPNCTK 
      SFTKQGHIV ISAAKSIQE GDHLSICYS DPLWGTPSR 
      RYFLHETKF FWCHCERCE DPSEFGTNF SAIKCSTKS 
      CGGYLLPAT FTELQKLPD WVCDKCDKT MSFYSVHDI 
      LERIGRDLF DLDKSSIED CKDFLNTHQ DLLHENHYY 
      CTEVKYTLS QLIGQRLIL VISDEDLSL KLQYCNSLE 
      KLLKIIAPA EKRALGLIL FEKYVALTE IARRAGPQK 
      ITDLEEAKK ILLETIELL KNEPDVLPE GKICKQAVQ 
      KLKDIDTAL QTLKSKEDV K
    CDS sequence
    • >TC033977 RA
      atggatccg accaatcga tgcgaagtg tgcgaaaag 
      ccggccttg cacaagtgc gggaaatgc cacaatgcc 
      cattactgc gacaaaaag catcagagg gagcattgg 
      aagcaacac aaaagtgtt tgccaaccg ttcaagata 
      tgcgaggat gcggtgttg gggcgccac ttggtggcc 
      actcgcgcc cttaaaccg ggggaggtg gtcatccag 
      gaagcgccc ctcatttgg ggcccgtcg tacaactcg 
      atccccgtg tgtctgggg tgcgggaaa gcggtcgac 
      gagacgtgc agcaggccg tgccagaag tgtggatgg 
      ccggtttgt agcgacctt tgcgaaaaa tcgccctcg 
      cacatacca gagtgtcgc tacacgagc caaaggggc 
      gagaaaatc accgtgaaa atattcggg gttccgcac 
      cccatttac aaatgtata acagtactt aggtgcttg 
      taccagaag cagttcttg cccaaaaac tggaaaaag 
      ttggaaact ttacaaaac cacttggag gaacacaaa 
      tcgacaacg aactacgaa agcgatcga gtgaccatt 
      gccgaattt attcgccga tttttcaag ttatcggca 
      actttctcc gaagaagac atcatgaaa gtgcatggt 
      agtaccttg gtcaatgga cacgaagta cctctaact 
      gagcccttc cacgtggcc atatattca agtgcttcg 
      atgctggaa cacagctgt ggtcccaac tgcaccaag 
      agcttcaca aagcaagga cacatcgta atttccgct 
      gccaaaagc atacaagaa ggggaccat ttgagtatt 
      tgttacagc gaccccttg tggggtact ccaagtcgg 
      aggtacttc ctccatgag acaaagttc ttctggtgc 
      cactgcgaa cggtgtgag gacccttct gagttcggt 
      actaacttt agtgcaatc aagtgttca accaagtct 
      tgtggtggg tacctcctg ccagccacg ttcacagag 
      ttgcaaaaa cttccagat tgggtttgt gataaatgt 
      gataagacc atgtcgttt tattcggtt catgatatt 
      ctggaacgg atcggtcgg gatctgttt gatttagac 
      aagagtagc attgaagat tgtaaggat tttttaaac 
      acccatcaa gatctttta cacgaaaat cactactac 
      tgtactgaa gttaaatac accctgtcc caactgatc 
      gggcagaga ctgattctt gtaatttcc gatgaggac 
      ttgtctttg aaattgcaa tactgcaac agtttagaa 
      aagttgctg aaaattatt gcaccggct gagaagaga 
      gctttgggg ttaattttg tttgagaag tatgtagct 
      ttaacggaa attgcgaga cgagcaggg ccacaaaaa 
      atcaccgac ttggaggag gcgaaaaaa attcttttg 
      gaaacgatt gaattgctc aaaaatgag cctgatgtt 
      cttcctgag ggtaaaata tgtaaacaa gccgttcag 
      aaattgaaa gatattgat acagcttta caaacgctt 
      aaaagtaaa gaagacgta aagtga
    mRNA sequence
    • >TC033977 RA
      gtagtagtt tatttatag tagcggcaa gtcggttga 
      attgcagtt ctgtgtgtt tcaattgca aagcgtggg 
      cttgtgagt tgcgtctaa ttttttatt attgtttgt 
      ttggattgc attcaaaaa tccgcaatg gatccgacc 
      aatcgatgc gaagtgtgc gaaaagccg gccttgcac 
      aagtgcggg aaatgccac aatgcccat tactgcgac 
      aaaaagcat cagagggag cattggaag caacacaaa 
      agtgtttgc caaccgttc aagatatgc gaggatgcg 
      gtgttgggg cgccacttg gtggccact cgcgccctt 
      aaaccgggg gaggtggtc atccaggaa gcgcccctc 
      atttggggc ccgtcgtac aactcgatc cccgtgtgt 
      ctggggtgc gggaaagcg gtcgacgag acgtgcagc 
      aggccgtgc cagaagtgt ggatggccg gtttgtagc 
      gacctttgc gaaaaatcg ccctcgcac ataccagag 
      tgtcgctac acgagccaa aggggcgag aaaatcacc 
      gtgaaaata ttcggggtt ccgcacccc atttacaaa 
      tgtataaca gtacttagg tgcttgtac cagaagcag 
      ttcttgccc aaaaactgg aaaaagttg gaaacttta 
      caaaaccac ttggaggaa cacaaatcg acaacgaac 
      tacgaaagc gatcgagtg accattgcc gaatttatt 
      cgccgattt ttcaagtta tcggcaact ttctccgaa 
      gaagacatc atgaaagtg catggtagt accttggtc 
      aatggacac gaagtacct ctaactgag cccttccac 
      gtggccata tattcaagt gcttcgatg ctggaacac 
      agctgtggt cccaactgc accaagagc ttcacaaag 
      caaggacac atcgtaatt tccgctgcc aaaagcata 
      caagaaggg gaccatttg agtatttgt tacagcgac 
      cccttgtgg ggtactcca agtcggagg tacttcctc 
      catgagaca aagttcttc tggtgccac tgcgaacgg 
      tgtgaggac ccttctgag ttcggtact aactttagt 
      gcaatcaag tgttcaacc aagtcttgt ggtgggtac 
      ctcctgcca gccacgttc acagagttg caaaaactt 
      ccagattgg gtttgtgat aaatgtgat aagaccatg 
      tcgttttat tcggttcat gatattctg gaacggatc 
      ggtcgggat ctgtttgat ttagacaag agtagcatt 
      gaagattgt aaggatttt ttaaacacc catcaagat 
      cttttacac gaaaatcac tactactgt actgaagtt 
      aaatacacc ctgtcccaa ctgatcggg cagagactg 
      attcttgta atttccgat gaggacttg tctttgaaa 
      ttgcaatac tgcaacagt ttagaaaag ttgctgaaa 
      attattgca ccggctgag aagagagct ttggggtta 
      attttgttt gagaagtat gtagcttta acggaaatt 
      gcgagacga gcagggcca caaaaaatc accgacttg 
      gaggaggcg aaaaaaatt cttttggaa acgattgaa 
      ttgctcaaa aatgagcct gatgttctt cctgagggt 
      aaaatatgt aaacaagcc gttcagaaa ttgaaagat 
      attgataca gctttacaa acgcttaaa agtaaagaa 
      gacgtaaag tgaaccaaa ttatttgtt taaagtttg 
      ttatgttta gttgtttat aaaaattta taaaaatgt 
      aaaacaaag gcgaaataa agaattaag aaaatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY