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Gene information
General information
TC006038 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2569443..2571397
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006038
    iBeetle screen iB_10431

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    Comment: only undefineable yolk remaining in oviduct
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006038 RA
      MGKSWAELG VKLHKEVQN AVKTLNFPT MTPIQAAAI 
      PQLLNKKDV VAEAVTGSG KTLAFLIPL LQILKQRDK 
      WKKAEIGAL IISPTRELA TQINQVLNQ LLESISGIT 
      SLLLVGGNS VEEDLNNLK CNGGNIIIC TPGRFEDLL 
      SRKSDFNLT LALKNLEIL ILDEADRLL DYGFRKSLD 
      VILSYLPKQ RRTGLFSAT QTKQLQDLI RAGLRNPVL 
      VSVSVKAEH STPEKLENY YVVANNNNK LAALFAFLQ 
      SRDVQKAML FLPTCAVVD FWSETFPCV FNDLPVLAI 
      HGKMKEKRK KIIERFKNS PKGLLLCTD VLARGIDIP 
      EVDWVLQWD PPASASAFV HRVGRTARQ GRQGSSLLF 
      LLDTEESYV PFIEKNQRV KLDQLSDVS DTKMCENLR 
      DILQNLQKT DRDLMEKAK RAFVSHVRA YSKYECSLL 
      LRIKEFPFG QVATSYGLL QLPKMPELK KYDSVGFEP 
      LEIDLNTIP YKDKQREKA RLQKLEIYQ NTGSWPGKP 
      KIVKKPSEP WSLTKQKKN EKKEKRLKR KRAKEKKRK 
      SGLSEEDLA ELAQDVALI KKCKKRKIS ENELDKSFG 
      LA
    CDS sequence
    • >TC006038 RA
      atgggcaaa tcgtgggcc gaactgggc gtaaaactc 
      cacaaggaa gtacaaaat gcggttaaa actctcaat 
      ttccccaca atgacccca attcaagcg gccgcaatt 
      ccccaatta ttaaacaag aaggatgta gtggctgaa 
      gctgtgaca ggatcgggg aaaacccta gccttcctc 
      atcccttta ctccaaata ctcaaacaa cgcgacaaa 
      tggaaaaaa gccgaaatt ggggcctta attatctca 
      ccgacacgt gaattagcc actcagatt aatcaagtt 
      ttgaaccaa ttgcttgaa tcgatatcg gggataact 
      tcgctactc ttagttggg ggcaattca gtcgaggag 
      gatttaaac aacttaaag tgcaacggt ggcaatatc 
      attatttgt acccctggt cgcttcgaa gacttactt 
      tcgcgcaaa agtgatttt aatttaacc ctggcctta 
      aaaaatctg gaaatatta atactggat gaagctgac 
      cggcttctc gactacggt tttcgcaag tctctggac 
      gtgatcctg agttattta ccgaaacag agacgtacc 
      ggtcttttc tcagccact cagaccaaa caattgcaa 
      gatttgatc agggccggg ctccgaaat cccgttctg 
      gtgagcgtc agtgtcaag gccgaacac agcacgccc 
      gagaaattg gaaaattat tacgtggtt gcgaataat 
      aataataaa ttagccgca cttttcgca tttttgcaa 
      agtcgggac gtgcaaaag gcgatgctg tttttgccg 
      acttgtgcc gtcgttgat ttttggtcg gaaacgttt 
      ccgtgtgtt ttcaacgat ttgcccgtt ttggcaatt 
      catgggaaa atgaaagag aagcgtaaa aaaatcata 
      gagaggttt aagaactcg cccaaggga ttattgctc 
      tgtaccgat gttttagca cgcggaatt gacattccg 
      gaggttgat tgggtcctt cagtgggat cccccggcc 
      agtgcgagt gctttcgtg catcgagtt ggccggact 
      gcacggcaa ggacgtcaa ggttcgtcg ttacttttc 
      ctcctagat acggaagaa tcctacgtt ccgttcatt 
      gaaaaaaat caaagagtc aaactcgat caactctct 
      gatgtttca gacacaaaa atgtgtgaa aatttgaga 
      gatatttta caaaatttg caaaaaacg gaccgcgat 
      ttaatggaa aaagccaaa cgggcgttc gtttcgcac 
      gtcagagcc tactcgaaa tacgaatgt tcgttatta 
      ttacgaatc aaagagttt cctttcggc caagtggcc 
      acaagttac ggtttactc caattaccg aaaatgccc 
      gaactgaaa aaatacgat tcggtcgga ttcgaacca 
      ctggaaatc gacctcaac acaatcccc tacaaagac 
      aaacaacgg gaaaaagca aggcttcaa aaacttgag 
      atttatcaa aacactggt tcctggccg gggaaaccc 
      aaaattgtg aaaaaacca agcgagccg tggtcgtta 
      accaaacaa aagaaaaac gaaaagaag gagaaacga 
      ctgaaacga aaacgagca aaagaaaaa aagaggaaa 
      agtgggcta agtgaagag gatttggcc gaattggca 
      caagatgtc gccctgata aaaaaatgc aagaagagg 
      aaaatctcg gaaaatgag ctcgataag agtttcgga 
      ctggcgtag
    mRNA sequence
    • >TC006038 RA
      accctacct ttttaacct cactttttg tggttgaaa 
      aaatgggca aatcgtggg ccgaactgg gcgtaaaac 
      tccacaagg aagtacaaa atgcggtta aaactctca 
      atttcccca caatgaccc caattcaag cggccgcaa 
      ttccccaat tattaaaca agaaggatg tagtggctg 
      aagctgtga caggatcgg ggaaaaccc tagccttcc 
      tcatccctt tactccaaa tactcaaac aacgcgaca 
      aatggaaaa aagccgaaa ttggggcct taattatct 
      caccgacac gtgaattag ccactcaga ttaatcaag 
      ttttgaacc aattgcttg aatcgatat cggggataa 
      cttcgctac tcttagttg ggggcaatt cagtcgagg 
      aggatttaa acaacttaa agtgcaacg gtggcaata 
      tcattattt gtacccctg gtcgcttcg aagacttac 
      tttcgcgca aaagtgatt ttaatttaa ccctggcct 
      taaaaaatc tggaaatat taatactgg atgaagctg 
      accggcttc tcgactacg gttttcgca agtctctgg 
      acgtgatcc tgagttatt taccgaaac agagacgta 
      ccggtcttt tctcagcca ctcagacca aacaattgc 
      aagatttga tcagggccg ggctccgaa atcccgttc 
      tggtgagcg tcagtgtca aggccgaac acagcacgc 
      ccgagaaat tggaaaatt attacgtgg ttgcgaata 
      ataataata aattagccg cacttttcg catttttgc 
      aaagtcggg acgtgcaaa aggcgatgc tgtttttgc 
      cgacttgtg ccgtcgttg atttttggt cggaaacgt 
      ttccgtgtg ttttcaacg atttgcccg ttttggcaa 
      ttcatggga aaatgaaag agaagcgta aaaaaatca 
      tagagaggt ttaagaact cgcccaagg gattattgc 
      tctgtaccg atgttttag cacgcggaa ttgacattc 
      cggaggttg attgggtcc ttcagtggg atcccccgg 
      ccagtgcga gtgctttcg tgcatcgag ttggccgga 
      ctgcacggc aaggacgtc aaggttcgt cgttacttt 
      tcctcctag atacggaag aatcctacg ttccgttca 
      ttgaaaaaa atcaaagag tcaaactcg atcaactct 
      ctgatgttt cagacacaa aaatgtgtg aaaatttga 
      gagatattt tacaaaatt tgcaaaaaa cggaccgcg 
      atttaatgg aaaaagcca aacgggcgt tcgtttcgc 
      acgtcagag cctactcga aatacgaat gttcgttat 
      tattacgaa tcaaagagt ttcctttcg gccaagtgg 
      ccacaagtt acggtttac tccaattac cgaaaatgc 
      ccgaactga aaaaatacg attcggtcg gattcgaac 
      cactggaaa tcgacctca acacaatcc cctacaaag 
      acaaacaac gggaaaaag caaggcttc aaaaacttg 
      agatttatc aaaacactg gttcctggc cggggaaac 
      ccaaaattg tgaaaaaac caagcgagc cgtggtcgt 
      taaccaaac aaaagaaaa acgaaaaga aggagaaac 
      gactgaaac gaaaacgag caaaagaaa aaaagagga 
      aaagtgggc taagtgaag aggatttgg ccgaattgg 
      cacaagatg tcgccctga taaaaaaat gcaagaaga 
      ggaaaatct cggaaaatg agctcgata agagtttcg 
      gactggcgt agaacgagg ttatgtaac gtgataaaa 
      ataaagcta ttttggtgg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY