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Gene information
General information
TC033882
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008608
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15907707..15923754
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033882
    iBeetle screen iB_10423

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033882 RA
      MKGVAAAVW LVCCVATLG DGVTFDTTT TKQLEEVIE 
      KNDFIVYIN HIKPKRRIV HNAETLFAV EFPGTDQKF 
      ELKLDRSTK RVIVETVED DRRRIQHFK VDSLHENSI 
      IKSLILAVN QTQPGAHAT LYINCVSYG MVATPKAMK 
      DMYVSMVEP RVEVLHDKK YPLFVDGHK DLRMVLSKN 
      NCPVQPEAD YDDSFSYLR DDPFIGPRK DHRADIPIL 
      DAIDTNALF DVINKLIIA VNRLDEEIG QHSQVIDHL 
      RHLIEECEL CKRRPPEYV RPTCATHNP CAPGVRCEE 
      TATGLRCGQ CPSGFFGNG YQCRRGRTC RDQPCFRGV 
      QCYEDSERG YRCGSCPQG YEGNGEVCQ RRNPCQENP 
      CGPGTLPHD YDN
    CDS sequence
    • >TC033882 RA
      atgaagggg gttgcagcg gccgtttgg ctcgtctgc 
      tgcgtcgcg acgcttgga gacggcgtt acttttgac 
      acaacaacg accaaacaa ctggaagaa gtgatcgaa 
      aagaatgat ttcatcgtt tatatcaat cacatcaaa 
      cccaaacga cgaattgta cataatgca gagacgctt 
      tttgcggtt gaatttcca ggaaccgat cagaaattt 
      gagcttaaa cttgataga agcacgaag cgagtcatt 
      gttgaaact gtggaggat gaccgtcgc cgaatccag 
      catttcaaa gttgactct cttcatgag aatagcatc 
      atcaaatcg ctgattctg gccgtaaat cagacacaa 
      ccaggagca catgccacg ttgtacata aactgtgtt 
      tcttatggg atggtggcc acccccaag gcgatgaaa 
      gacatgtat gtgtctatg gtagagcca agagtagaa 
      gtgctccat gacaaaaaa taccccttg ttcgtcgac 
      ggccacaaa gacctccgc atggtcctc agcaaaaac 
      aactgcccc gtccaaccg gaagcagac tacgacgac 
      tccttcagc tacctccgt gatgaccct ttcattggc 
      ccccgtaaa gaccaccgc gctgacatc cccatcctc 
      gacgctatt gacacgaat gccctcttc gacgtcatc 
      aacaaacta atcatcgcc gtcaaccgc ctcgacgaa 
      gaaattggt caacactcg caagtcatc gaccattta 
      cgccacctc attgaggaa tgcgaattg tgcaaacgc 
      cgacccccg gagtacgtg cgccctacc tgcgccacc 
      cacaacccg tgcgctccg ggcgtacgc tgcgaagag 
      accgccaca ggtctgagg tgcggccag tgtccatca 
      ggattcttt ggaaatggg tatcagtgc aggagagga 
      cgcacgtgt agagatcag ccctgcttt agaggagtg 
      caatgttat gaagactcg gagagaggg taccgatgt 
      ggaagctgt ccgcaagga tatgagggt aatggcgaa 
      gtttgccaa agacggaat ccttgccag gagaacccc 
      tgtgggcca ggtacgttg ccccatgac tatgataat 
      tag
    mRNA sequence
    • >TC033882 RA
      acgcatatc tcaacgaca ttattgcta caattggaa 
      agtgttttt gttgtggtc ttctataaa tggatatta 
      caactgggc cacgcatcc atgccatca caatgcgac 
      taacagatg tttatgtaa atcgaggct cttttctga 
      aggctttcc gtccaattc ccgaaattt attcaacac 
      acctagaga gagaactgg acaataatt ggctggtgg 
      tggaacaaa taaggagca tgtctccga tgacgaaat 
      cctacacat cattccaag taagtccac taagagagc 
      acttgtcca gtcgcgagc aaggcgcta agctgtgaa 
      gtgactgtg aagaagaga tgaaggggg ttgcagcgg 
      ccgtttggc tcgtctgct gcgtcgcga cgcttggag 
      acggcgtta cttttgaca caacaacga ccaaacaac 
      tggaagaag tgatcgaaa agaatgatt tcatcgttt 
      atatcaatc acatcaaac ccaaacgac gaattgtac 
      ataatgcag agacgcttt ttgcggttg aatttccag 
      gaaccgatc agaaatttg agcttaaac ttgatagaa 
      gcacgaagc gagtcattg ttgaaactg tggaggatg 
      accgtcgcc gaatccagc atttcaaag ttgactctc 
      ttcatgaga atagcatca tcaaatcgc tgattctgg 
      ccgtaaatc agacacaac caggagcac atgccacgt 
      tgtacataa actgtgttt cttatggga tggtggcca 
      cccccaagg cgatgaaag acatgtatg tgtctatgg 
      tagagccaa gagtagaag tgctccatg acaaaaaat 
      accccttgt tcgtcgacg gccacaaag acctccgca 
      tggtcctca gcaaaaaca actgccccg tccaaccgg 
      aagcagact acgacgact ccttcagct acctccgtg 
      atgaccctt tcattggcc cccgtaaag accaccgcg 
      ctgacatcc ccatcctcg acgctattg acacgaatg 
      ccctcttcg acgtcatca acaaactaa tcatcgccg 
      tcaaccgcc tcgacgaag aaattggtc aacactcgc 
      aagtcatcg accatttac gccacctca ttgaggaat 
      gcgaattgt gcaaacgcc gacccccgg agtacgtgc 
      gccctacct gcgccaccc acaacccgt gcgctccgg 
      gcgtacgct gcgaagaga ccgccacag gtctgaggt 
      gcggccagt gtccatcag gattctttg gaaatgggt 
      atcagtgca ggagaggac gcacgtgta gagatcagc 
      cctgcttta gaggagtgc aatgttatg aagactcgg 
      agagagggt accgatgtg gaagctgtc cgcaaggat 
      atgagggta atggcgaag tttgccaaa gacggaatc 
      cttgccagg agaacccct gtgggccag gtacgttgc 
      cccatgact atgataatt agttgcttg tgtgactag 
      tctttctcc aaaatggta attaataaa ttcctaatg 
      ttttcgttc tttcgagtt ataatactt tcagaaatt 
      gactttaaa ggaatgctc aactacaaa aaaaaaact 
      aaaaaaaaa ctagattac atttaagac taaaaggga 
      gcaagttgt agcgtgcga ttttatttt tctattata 
      tttttaact attcaccat cagaaaaaa accggaaga 
      aatttgtat ataaagtat tatttgtaa atatttagt 
      tgtggaaaa aaaatctgg gacaagtct ttaagcaga 
      ttttcaata aaatttaac cgactacga gtaaaacgg 
      acattgctc caaagtaat caaaatgat cattgtcga 
      catttaaaa tttagttaa aatgaaaat aatgtctaa 
      agctaagga cggttggac aataaggcg acttgtccc 
      ctgttacaa tgatgctaa aactttggt ctttactat 
      ttaaaaaaa aaaactacg acaatagga aaaaataaa 
      tagaaatcc tgcgtctgg acaaagaaa ggtttctaa 
      tcaagagat agatgaatt tctgcaggc atttaattt 
      catgttaca gcagcaaaa taatttatt tttgtccac 
      aactgtatt accaacaaa gtacttttt aattctagg 
      actttaaaa tttttcata atttttatg gtaaaaaag 
      gttttaaaa aacatggtg cggcgtcta atctctgtt 
      attacaaat tgtgtttat ccacaacca gtttattat 
      ttgtattat tactattat taggttata atttcgtaa 
      tactaccac tactaagag atatgatgg gtattaata 
      aaacacgga ttctatgaa agcaccata aaacacgtt 
      acaaaatta aattgtatt taaatttta ttattaaga 
      tatatgcat ttaaattta gcaataagt atcaatatt 
      tctctacac cttcaatgt cttcaagat ggagtattt 
      agtccaaga tgacaaaga ggcgtactt ttagtgata 
      ttcaagaaa tacgtcaag aacaactct tcggtagtt 
      ctaatcact gatcttgca catcctctt tttttcttc 
      caatttata atctcagaa aattccttt ttcgtaagc 
      ttcgtttga cttctttgg caataattc aaaagttga 
      catttctgc ttcagcaag aatttcatc tggaatgct 
      ttctttaat ctcaatagt ttccatttg tcaaaactg 
      aattgaact taattattg caaagtgta aaatgaagt 
      caaaaacca tttaaaaat aagtagcaa ccgttttat 
      gactagccc tgctcactt ccgaataaa atttaaaac 
      cgtca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY