Status
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Gene information
General information
TC034812 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13909953..13923565
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034812
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10412

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva circumference decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva rounded - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs sometimes fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034812 RA
      MPHTGQAGV NQLGGVFVN GRPLPDCVR RRIVELALL 
      GVRPCDISR QLLVSHGCV SKILTRFYE TGSIRPGSI 
      GGSKTKQVA TPTVVKKIL RFKQENPGM FAWEIREQL 
      IAQRVCEPH NIPSVSSVN RILRNSGVW PDPPEILHH 
      PRPPPTDSL MRGEMYSKM STNLTTLPY FSMHDSTYP 
      SNRLAALQH QLHIATSTP LDLPSANNW SNLIIPYHP 
      PSHEPSIQD EKECVISTE SNEKQQELK RKNPYSIEE 
      LLKKPDKKA KPPTVTYVG IQQPFGVFL THDAEEKND 
      SDVEKEVKV DVEVRSVRI RLDCIEYVK WNRCLRFVK 
      YDNYNVIYS HEESIFTAK RGVKTYRTG EFAYPFRIF 
      IPRYLPSTF THTYGKVFY LITGTVIPF IWNDFIDTS 
      IVVVQSPVE LYGITRSFQ REKIYYNET KFKRWKYGK 
      EGKLVMQVR VPRIAFVPG DVVDVKIYV RNESNQTVK 
      NLKVKFLKR VKFKSVNPK NKSKRHVET ILKFKKEGI 
      GAYKEHTYD LRLNFPKVL DIPNLFSCT LIKLEFSLK 
      IWCQMPPFI RNFAIEIHP EMGHFFSGC VSQFRFKLV 
      K
    CDS sequence
    • >TC034812 RA
      atgcctcac acaggtcaa gcaggtgtc aaccaatta 
      ggcggagtg tttgtgaac ggtcgtcct ttgcctgat 
      tgcgtgcga agaaggata gtcgaactt gcattattg 
      ggagttcgg ccttgtgat atctcgaga cagttgcta 
      gtttctcat ggatgtgtt tcgaaaatt cttacacgc 
      ttttacgaa acagggtcc atccgcccg gggtccata 
      gggggctcc aagacgaag caagtggcc acccccacc 
      gtcgtcaag aaaatcctc aggttcaaa caggaaaat 
      cccggaatg ttcgcttgg gaaatccgg gagcagctc 
      atcgctcag cgcgtttgt gagccacat aacataccc 
      tcagtcagc tccgtcaat cggatattg aggaacagt 
      ggtgtgtgg ccggaccct cctgaaata ttgcaccac 
      cctcgaccg ccacctaca gattctcta atgcgagga 
      gaaatgtat tcgaaaatg tcgactaat ctgactact 
      ctcccttac ttctcaatg cacgactcg acttaccca 
      agtaacaga ttagcagct ctccaacac cagcttcac 
      atagcgact tcaactcct ctagacttg ccctcggct 
      aacaattgg tcaaattta ataattccc tatcacccg 
      cccagccat gaaccctca atccaggac gagaaagaa 
      tgcgttata tcgaccgaa agtaacgaa aaacaacaa 
      gaactcaaa agaaaaaat ccctactct atcgaggaa 
      ctcctcaaa aagcccgat aaaaaagcc aaacctccc 
      actgtcaca tacgtgggg attcagcaa ccctttggc 
      gtgtttctc acacacgat gcggaggaa aaaaatgat 
      agtgatgtt gagaaggaa gttaaagtt gatgttgaa 
      gtcagaagt gtgagaatt cgattagat tgcatcgaa 
      tacgtgaaa tggaacaga tgtcttcgc tttgtaaag 
      tacgacaat tacaacgtg atttactcc cacgaagaa 
      agcattttt accgcgaaa cggggagtc aaaacctac 
      cgcacagga gaattcgct taccccttt cgcattttc 
      atacctcgc tatttgcca tcaacattc acccacact 
      tacggaaaa gtgttttat ctgataaca gggaccgta 
      attcccttc atttggaac gatttcata gacacgtct 
      atagttgtg gtccaatcc ccagttgaa ctttacgga 
      ataactagg agttttcaa agagagaaa atctattac 
      aacgaaacg aaattcaag cgttggaaa tacggcaaa 
      gaagggaaa ttggtgatg caagtgcgg gtgcctcga 
      attgcgttc gtgcccggc gacgttgtt gatgttaaa 
      atttacgtg aggaatgag tctaatcag actgtaaag 
      aatttaaaa gttaaattt ttgaaacga gtcaagttt 
      aaatcggtc aatcccaaa aacaaaagt aagagacat 
      gtcgaaacg atactgaag ttcaaaaag gaagggatc 
      ggggcttat aaagaacac acctatgat ttgagatta 
      aatttcccg aaagtcttg gatataccg aatttgttt 
      tcctgtact ttgattaaa ctagagttt tcgctgaaa 
      atatggtgc caaatgccc ccgtttatt cgaaatttc 
      gccattgaa atccatcca gaaatggga cactttttc 
      agtgggtgc gtaagccag ttccgtttt aaattagtg 
      aaataa
    mRNA sequence
    • >TC034812 RA
      gacggcggc aggacattc acgcactcg aaggactta 
      cacacgcac tccaggtgg agggcttga gacggacac 
      attatgcct cacacaggt caagcaggt gtcaaccaa 
      ttaggcgga gtgtttgtg aacggtcgt cctttgcct 
      gattgcgtg cgaagaagg atagtcgaa cttgcatta 
      ttgggagtt cggccttgt gatatctcg agacagttg 
      ctagtttct catggatgt gtttcgaaa attcttaca 
      cgcttttac gaaacaggg tccatccgc ccggggtcc 
      atagggggc tccaagacg aagcaagtg gccaccccc 
      accgtcgtc aagaaaatc ctcaggttc aaacaggaa 
      aatcccgga atgttcgct tgggaaatc cgggagcag 
      ctcatcgct cagcgcgtt tgtgagcca cataacata 
      ccctcagtc agctccgtc aatcggata ttgaggaac 
      agtggtgtg tggccggac cctcctgaa atattgcac 
      caccctcga ccgccacct acagattct ctaatgcga 
      ggagaaatg tattcgaaa atgtcgact aatctgact 
      actctccct tacttctca atgcacgac tcgacttac 
      ccaagtaac agattagca gctctccaa caccagctt 
      cacatagcg acttcaact cctctagac ttgccctcg 
      gctaacaat tggtcaaat ttaataatt ccctatcac 
      ccgcccagc catgaaccc tcaatccag gacgagaaa 
      gaatgcgtt atatcgacc gaaagtaac gaaaaacaa 
      caagaactc aaaagaaaa aatccctac tctatcgag 
      gaactcctc aaaaagccc gataaaaaa gccaaacct 
      cccactgtc acatacgtg gggattcag caacccttt 
      ggcgtgttt ctcacacac gatgcggag gaaaaaaat 
      gatagtgat gttgagaag gaagttaaa gttgatgtt 
      gaagtcaga agtgtgaga attcgatta gattgcatc 
      gaatacgtg aaatggaac agatgtctt cgctttgta 
      aagtacgac aattacaac gtgatttac tcccacgaa 
      gaaagcatt tttaccgcg aaacgggga gtcaaaacc 
      taccgcaca ggagaattc gcttacccc tttcgcatt 
      ttcatacct cgctatttg ccatcaaca ttcacccac 
      acttacgga aaagtgttt tatctgata acagggacc 
      gtaattccc ttcatttgg aacgatttc atagacacg 
      tctatagtt gtggtccaa tccccagtt gaactttac 
      ggaataact aggagtttt caaagagag aaaatctat 
      tacaacgaa acgaaattc aagcgttgg aaatacggc 
      aaagaaggg aaattggtg atgcaagtg cgggtgcct 
      cgaattgcg ttcgtgccc ggcgacgtt gttgatgtt 
      aaaatttac gtgaggaat gagtctaat cagactgta 
      aagaattta aaagttaaa tttttgaaa cgagtcaag 
      tttaaatcg gtcaatccc aaaaacaaa agtaagaga 
      catgtcgaa acgatactg aagttcaaa aaggaaggg 
      atcggggct tataaagaa cacacctat gatttgaga 
      ttaaatttc ccgaaagtc ttggatata ccgaatttg 
      ttttcctgt actttgatt aaactagag ttttcgctg 
      aaaatatgg tgccaaatg cccccgttt attcgaaat 
      ttcgccatt gaaatccat ccagaaatg ggacacttt 
      ttcagtggg tgcgtaagc cagttccgt tttaaatta 
      gtgaaataa ttatttaat aaactaatt cgtttttgc 
      tttaagcag taccgatta ttattaaac acccatttt 
      tttagtcgg tatatatta atcaataat tcaaatgat 
      cacattggt ccataaaaa aattaaaaa ttctacaaa 
      ttaaacaaa tatggaaat t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY