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Gene information
General information
TC010771 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4989466..4992180
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010771
    iBeetle screen iB_10411

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs segment sometimes number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs sometimes size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010771 RA
      MLSDTDVED HYSPSVRLI TLERNGSNC GFHLTRSKW 
      DPYPWVNSV EDGTAADSA GVKPGDCLL EVNGEDVVG 
      QRISEVAEI VKSKPNQVS LLLWNAGVD PQCTPEAVC 
      CGPMPTNLQ RLSACMSTI LAFLECPVC LDTIPPPTY 
      QCENGHLIC IRCRAKSER CPICRLRFS RGRSLLADQ 
      VYNALVDAF NLREEPDDS RTAKMHQIF KMKTKKNVP 
      DIKVTPSHT TKFLARLVG KASSVDNLS GSANDSDFN 
      LRTKSLSNQ EIFDAGSPL VSRTNSVNR MSRNEKNQN 
      YLNPNFRSV SCHGSFESL HNAGEDIAF YCPFAPGCT 
      SLIKGKKVI EHFQLSHDG PLVHYFKSQ ITIEFSKIL 
      EEQDLCYVI QLQQNTFFL KIENSSENV LLWTWVLEN 
      GNVAKEFDM HVKVLIGET HDVIISVRN NVFSLNCTS 
      YQEIKGLKK GLLVNTRAI NGAINDVIF DIAILKSDV
    CDS sequence
    • >TC010771 RA
      atgttgagt gacacagac gttgaagac cactatagt 
      ccgtctgtg cggctgatc actcttgag agaaatggc 
      tccaattgc ggattccac ctaaccagg agcaaatgg 
      gacccctat ccgtgggtc aactctgtc gaggatggc 
      acagctgcc gactccgcg ggcgtcaag cccggggac 
      tgcctgctg gaagtcaac ggcgaggat gtcgtgggc 
      cagaggatc agtgaagtg gccgaaata gtgaaatca 
      aagcccaat caagtttca cttttgttg tggaatgcc 
      ggtgtcgat cctcagtgt acgcccgag gcagtttgc 
      tgcggaccc atgccaacg aacctccag cgcttgtct 
      gcatgcatg tctacgatt cttgcgttt ttggagtgt 
      ccagtctgc ctagacaca atccctcca cccacctac 
      cagtgcgaa aacggccac ctgatttgc atacgctgt 
      agggcgaag tccgagagg tgccccatc tgtcgctta 
      agattcagc agaggtaga tcattacta gcagaccag 
      gtctacaat gcgttggtt gacgccttc aacctgcgg 
      gaagaacca gacgacagt cggaccgcg aaaatgcat 
      cagattttc aaaatgaaa accaagaaa aacgtgcct 
      gatatcaaa gtgaccccg tcccacaca acgaaattt 
      ttggcccga cttgtcgga aaagcgagc tcggttgat 
      aatctgtcc ggaagcgca aatgatagt gactttaat 
      ctgaggaca aagtcgctc tcaaaccag gaaattttc 
      gacgcgggg agtcctttg gtgtcacgg acgaactcc 
      gtgaacagg atgagccga aatgagaaa aaccaaaat 
      tatctcaac ccaaatttt cgctcagtg tcgtgtcac 
      ggctccttt gagtcgttg cacaacgcc ggtgaagac 
      atcgctttt tattgccca ttcgcgccg ggatgtacg 
      tccctaatt aaaggaaaa aaagtaatc gagcacttc 
      cagttaagc cacgacgga ccgttagta cactatttc 
      aaatcgcaa ataacaatc gagtttagc aaaattttg 
      gaagagcaa gacttgtgt tacgttatc caacttcag 
      caaaacacg tttttcctc aaaattgag aacagttcc 
      gaaaatgta cttctctgg acttgggtt ctagaaaac 
      ggaaatgtt gcaaaagaa ttcgacatg cacgtcaag 
      gtgttaatt ggggaaaca cacgacgtt ataataagt 
      gttagaaat aacgtgttt tcactcaac tgcacttca 
      taccaagag ataaagggg ctaaaaaaa ggtttactt 
      gttaatact cgggccata aatggcgcg ataaatgat 
      gttattttt gatatagcg attttaaaa agtgacgta 
      tga
    mRNA sequence
    • >TC010771 RA
      tttataccc acgtttatt caagtccag catgttgag 
      tgacacaga cgttgaaga ccactatag tccgtctgt 
      gcggctgat cactcttga gagaaatgg ctccaattg 
      cggattcca cctaaccag gagcaaatg ggaccccta 
      tccgtgggt caactctgt cgaggatgg cacagctgc 
      cgactccgc gggcgtcaa gcccgggga ctgcctgct 
      ggaagtcaa cggcgagga tgtcgtggg ccagaggat 
      cagtgaagt ggccgaaat agtgaaatc aaagcccaa 
      tcaagtttc acttttgtt gtggaatgc cggtgtcga 
      tcctcagtg tacgcccga ggcagtttg ctgcggacc 
      catgccaac gaacctcca gcgcttgtc tgcatgcat 
      gtctacgat tcttgcgtt tttggagtg tccagtctg 
      cctagacac aatccctcc acccaccta ccagtgcga 
      aaacggcca cctgatttg catacgctg tagggcgaa 
      gtccgagag gtgccccat ctgtcgctt aagattcag 
      cagaggtag atcattact agcagacca ggtctacaa 
      tgcgttggt tgacgcctt caacctgcg ggaagaacc 
      agacgacag tcggaccgc gaaaatgca tcagatttt 
      caaaatgaa aaccaagaa aaacgtgcc tgatatcaa 
      agtgacccc gtcccacac aacgaaatt tttggcccg 
      acttgtcgg aaaagcgag ctcggttga taatctgtc 
      cggaagcgc aaatgatag tgactttaa tctgaggac 
      aaagtcgct ctcaaacca ggaaatttt cgacgcggg 
      gagtccttt ggtgtcacg gacgaactc cgtgaacag 
      gatgagccg aaatgagaa aaaccaaaa ttatctcaa 
      cccaaattt tcgctcagt gtcgtgtca cggctcctt 
      tgagtcgtt gcacaacgc cggtgaaga catcgcttt 
      ttattgccc attcgcgcc gggatgtac gtccctaat 
      taaaggaaa aaaagtaat cgagcactt ccagttaag 
      ccacgacgg accgttagt acactattt caaatcgca 
      aataacaat cgagtttag caaaatttt ggaagagca 
      agacttgtg ttacgttat ccaacttca gcaaaacac 
      gtttttcct caaaattga gaacagttc cgaaaatgt 
      acttctctg gacttgggt tctagaaaa cggaaatgt 
      tgcaaaaga attcgacat gcacgtcaa ggtgttaat 
      tggggaaac acacgacgt tataataag tgttagaaa 
      taacgtgtt ttcactcaa ctgcacttc ataccaaga 
      gataaaggg gctaaaaaa aggtttact tgttaatac 
      tcgggccat aaatggcgc gataaatga tgttatttt 
      tgatatagc gattttaaa aagtgacgt atgagctgt 
      gatattgtt tctaattta taaatattt atttggaga 
      tattgtttg tttgaattc gttaataat aaaacggtt 
      ttttcgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY