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Gene information
General information
TC008848 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10743523..10747405
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008848
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10375

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008848 RA
      MPKTRKFID KKKAITFQL VHRSQQDPL IADENAPQR 
      VLVPIHEKE KRLEEQHKY GIYYNDDCN YLEHLKDSK 
      DNQVEWPDH VEKSLKERR VKLDLPATV FASKHEEKE 
      GMLSKAAPV SGPQLQLDP DLVAAMDED FDYSDPENQ 
      LEDNFIELA EGVASDQEF DDEFEVESD LGSEERDEV 
      GSLPDSQLS FNEEETKSR FTSYSMTSS VIRRNEQLT 
      LLDDRFEQM YADYDDNEI GALDCEEIE GHVPESSDI 
      LLQYAQEFE KSQQNEKLD KEVIKAKID ESLTKDSKS 
      EEEMVYMEV PQKEKWDCE SILSTYSNI YNHPKLIKE 
      PAKEKIQIS KKTGIPMNI LGSNKLTQK ALDKLNLEN 
      EFIESRGPK STGVQSVIS QLSTLSVRP RGETPDERR 
      DRKKLLKDY KRERRLEKK ANALAFKEE AKRQVKISM 
      NNRNNVQGN KIL
    CDS sequence
    • >TC008848 RA
      atgccaaag acacggaaa tttatcgat aaaaagaag 
      gcgattacc ttccaactg gtccacaga agccagcag 
      gacccccta atagcggac gagaacgct cctcagaga 
      gtcctcgtc ccaatacat gagaaagaa aaacgcttg 
      gaggagcaa cataaatat ggaatttac tacaacgac 
      gactgcaac tatttggaa cacttgaaa gactccaaa 
      gacaatcaa gtagaatgg cccgaccat gtggaaaag 
      tctctcaaa gagagaagg gtcaagctg gacttgcct 
      gccacagtt tttgcctca aaacatgag gaaaaagag 
      ggaatgttg agcaaggcc gctccagtt tcagggcct 
      caacttcaa ttggaccct gatttggtt gcggcaatg 
      gacgaagat tttgattac tcagatcct gagaatcag 
      ttggaggat aattttatt gagttagct gaaggtgtt 
      gcttctgat caagaattt gatgatgag tttgaagtg 
      gaatctgat ttgggcagt gaagagaga gatgaagtt 
      ggatcttta ccagactct caactctct ttcaacgaa 
      gaagaaaca aagtcaaga tttacaagt tattccatg 
      actagttct gttataagg aggaacgag cagctcact 
      ttgctcgat gatagattt gaacagatg tatgctgat 
      tatgacgac aacgaaatt ggggctttg gactgcgaa 
      gagatagaa gggcatgta cctgaaagc tctgacata 
      ctcctacaa tatgcccaa gaatttgaa aagagccaa 
      caaaatgaa aaattagat aaggaagtt ataaaggcg 
      aaaattgat gaaagctta actaaagac tctaaaagt 
      gaggaggag atggtgtac atggaagta ccgcagaag 
      gaaaaatgg gattgtgag tctatcctg agcacctac 
      tccaatatt tacaaccac ccaaaactg atcaaggaa 
      ccagctaag gaaaaaatt caaatcagt aagaaaacc 
      ggcattccg atgaacatt ttgggttcg aataaattg 
      actcaaaag gctttagat aagctgaat ctagagaac 
      gaatttatt gaaagtagg ggcccaaaa tcaacagga 
      gtgcagtct gtgatttcg caattgtcc acactttca 
      gtgcggcca aggggcgaa actccggac gagagaaga 
      gacaggaaa aaacttttg aaggattat aaaagagag 
      cgccgactt gagaaaaaa gccaatgct ttagcattt 
      aaagaagag gcaaagaga caagttaaa ataagcatg 
      aataaccgg aataatgtt caagggaat aaaattttg 
      taa
    mRNA sequence
    • >TC008848 RA
      gcagacata acctaattt tttatcatt atttaactt 
      ttcccaact tcgggagac tataaaagc atcaatttt 
      gttggaaag ggtttctgt gaatatttt tgcaaatat 
      tgataacac agaaaatgc caaagacac ggaaattta 
      tcgataaaa agaaggcga ttaccttcc aactggtcc 
      acagaagcc agcaggacc ccctaatag cggacgaga 
      acgctcctc agagagtcc tcgtcccaa tacatgaga 
      aagaaaaac gcttggagg agcaacata aatatggaa 
      tttactaca acgacgact gcaactatt tggaacact 
      tgaaagact ccaaagaca atcaagtag aatggcccg 
      accatgtgg aaaagtctc tcaaagaga gaagggtca 
      agctggact tgcctgcca cagtttttg cctcaaaac 
      atgaggaaa aagagggaa tgttgagca aggccgctc 
      cagtttcag ggcctcaac ttcaattgg accctgatt 
      tggttgcgg caatggacg aagattttg attactcag 
      atcctgaga atcagttgg aggataatt ttattgagt 
      tagctgaag gtgttgctt ctgatcaag aatttgatg 
      atgagtttg aagtggaat ctgatttgg gcagtgaag 
      agagagatg aagttggat ctttaccag actctcaac 
      tctctttca acgaagaag aaacaaagt caagattta 
      caagttatt ccatgacta gttctgtta taaggagga 
      acgagcagc tcactttgc tcgatgata gatttgaac 
      agatgtatg ctgattatg acgacaacg aaattgggg 
      ctttggact gcgaagaga tagaagggc atgtacctg 
      aaagctctg acatactcc tacaatatg cccaagaat 
      ttgaaaaga gccaacaaa atgaaaaat tagataagg 
      aagttataa aggcgaaaa ttgatgaaa gcttaacta 
      aagactcta aaagtgagg aggagatgg tgtacatgg 
      aagtaccgc agaaggaaa aatgggatt gtgagtcta 
      tcctgagca cctactcca atatttaca accacccaa 
      aactgatca aggaaccag ctaaggaaa aaattcaaa 
      tcagtaaga aaaccggca ttccgatga acattttgg 
      gttcgaata aattgactc aaaaggctt tagataagc 
      tgaatctag agaacgaat ttattgaaa gtaggggcc 
      caaaatcaa caggagtgc agtctgtga tttcgcaat 
      tgtccacac tttcagtgc ggccaaggg gcgaaactc 
      cggacgaga gaagagaca ggaaaaaac ttttgaagg 
      attataaaa gagagcgcc gacttgaga aaaaagcca 
      atgctttag catttaaag aagaggcaa agagacaag 
      ttaaaataa gcatgaata accggaata atgttcaag 
      ggaataaaa ttttgtaat ttgttgtat tattggcga 
      ttttgttta aataaagtt agaggaaat ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY