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Gene information
General information
TC009938 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11537376..11538890
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009938
    iBeetle screen iB_10364

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009938 RA
      MEQTTTQQV NVYRKMLAA SVCSILMES GFDTADKES 
      LGTLTEMIQ CFLTEVGAL SKSYCELSG RSEPVVADV 
      ILAFVEMGH NFNDLMVYT KGMKHSVLP PLQPQQPQK 
      QLNMLPAGS KHPLPPYIP QHLPQFPDP HAYVRTPTH 
      KQPIIDYES VREKAAVQK KDIEKALTK FLAKTNPTH 
      NLFDTDEAN IFPLIACKP AYPPYLSAL LPQDQIFDP 
      EDLEYDHKS QMMQQSKEQ KAKEKAENK EEEETEETL 
      NDSTKSEEN TNPNGYIDN PYLAATKLP KKDEMEVS
    CDS sequence
    • >TC009938 RA
      atggaacaa accaccacc cagcaagta aacgtttac 
      cgaaaaatg ttagcagcg tcagtctgc agcattcta 
      atggagtct ggtttcgat acggccgac aaagaaagc 
      ctcggtact ttaacagaa atgatccag tgcttccta 
      accgaagtg ggggcactt tcgaaaagc tactgcgag 
      ttgtcgggc cgttcggag cccgtggtg gccgacgtg 
      attttagca tttgtggaa atggggcac aatttcaac 
      gacttgatg gtgtacaca aaaggcatg aagcattcg 
      gttttaccc ccactacaa ccacagcag ccccagaag 
      cagctaaat atgttacca gctggctcg aagcaccct 
      ttaccccct tacatccct cagcattta ccccagttt 
      ccggacccc cacgcctac gttcgcact cctacacac 
      aaacaacca attattgat tacgaatca gtgagagaa 
      aaagcggca gttcagaaa aaagacata gaaaaggcg 
      ttaacgaag tttcttgcc aagactaac cccacacat 
      aatttgttt gacacagat gaggcgaat atttttcca 
      ctgatagct tgtaagcct gcgtacccc ccttacttg 
      agcgctctc ctgccccag gatcaaatt tttgatcct 
      gaagacctc gaatatgac cacaaaagt caaatgatg 
      caacaatca aaagaacaa aaagcgaaa gaaaaagct 
      gaaaataaa gaagaagaa gagactgaa gaaacacta 
      aatgattcc acaaaaagt gaagaaaac acaaaccca 
      aatgggtac attgataat ccgtatttg gccgcaacg 
      aagctgcct aaaaaggac gaaatggag gtgtcataa
    mRNA sequence
    • >TC009938 RA
      agaaacaca agcttgtca tttgtcaaa cgttattat 
      aaccttaac ttttcggaa cgtaaataa caacaaact 
      ttgctttaa actttaaaa aatggaaca aaccaccac 
      ccagcaagt aaacgttta ccgaaaaat gttagcagc 
      gtcagtctg cagcattct aatggagtc tggtttcga 
      tacggccga caaagaaag cctcggtac tttaacaga 
      aatgatcca gtgcttcct aaccgaagt gggggcact 
      ttcgaaaag ctactgcga gttgtcggg ccgttcgga 
      gcccgtggt ggccgacgt gattttagc atttgtgga 
      aatggggca caatttcaa cgacttgat ggtgtacac 
      aaaaggcat gaagcattc ggttttacc cccactaca 
      accacagca gccccagaa gcagctaaa tatgttacc 
      agctggctc gaagcaccc tttaccccc ttacatccc 
      tcagcattt accccagtt tccggaccc ccacgccta 
      cgttcgcac tcctacaca caaacaacc aattattga 
      ttacgaatc agtgagaga aaaagcggc agttcagaa 
      aaaagacat agaaaaggc gttaacgaa gtttcttgc 
      caagactaa ccccacaca taatttgtt tgacacaga 
      tgaggcgaa tatttttcc actgatagc ttgtaagcc 
      tgcgtaccc cccttactt gagcgctct cctgcccca 
      ggatcaaat ttttgatcc tgaagacct cgaatatga 
      ccacaaaag tcaaatgat gcaacaatc aaaagaaca 
      aaaagcgaa agaaaaagc tgaaaataa agaagaaga 
      agagactga agaaacact aaatgattc cacaaaaag 
      tgaagaaaa cacaaaccc aaatgggta cattgataa 
      tccgtattt ggccgcaac gaagctgcc taaaaagga 
      cgaaatgga ggtgtcata agtaagact aaattataa 
      gtttaaatt ttttattta tacaacatg tagttattt 
      ataagaatc ggagttata gaaatgaat ataacaaga 
      tttgaatca aacacaatt ttgttgctt gttgcaaac 
      aagaatggc tggaacaaa gcctcctct tccaaactt 
      gtcatccat ccgaccaca ctttcattc cgattttgt 
      actttgagc cacaacatc aagggctgg aagtactga 
      agcattgct tcagcagac aggcgatcc gactgtcct 
      ctagttaac atttttatc acttctttc caatgtctg 
      gccttcccg acttttagc aagtc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY