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Gene information
General information
TC009926 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11758514..11762791
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009926
    iBeetle screen iB_10363

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009926 RA
      MEIEAVKVD DVLEIFNTI QQSSGAHLH YATILKNAL 
      SNENDSELF STFCKCVEK AFCGSCKED IYNGRIFSF 
      VLVLAATFH PKKDDVIHP VLRKIIQHL IALSYLDNE 
      PLRFWSCKL INGIMKNVD EIDDELFEE FKNAMIERL 
      KDPKVAIRG QAILAVHRL QDPSDKNDP ITRQLCTYI 
      NSDSNGKLR QVCVEKVAI NKHVLTLVL ERLRDINLD 
      VRLAAFEKI RLLIKYLNL SQRHYILQC GFNDPSQKV 
      HDFVSTDLM KSWLEFCNN DYVRLINTI RLDASEDDI 
      EKTTIYAEQ MLRALFKNA PVNDITSCL LSQYLNEQK 
      LIEYEKLNW DIILYYRIV VQFLRQSPE FEETLNSIL 
      PELVLFCKY IRGYIEFIK TKQDLDELE YHFTLKQFF 
      IITETYDVS DSASRQCLN SLVHDALKK EVLPTSVEE 
      TIVRNLEKT FPDTNTRLV FVCEIISDI FYGEDEMSF 
      EEFKRQEME KSHTISQLK SDIALLSEQ EKYCASVEK 
      NYSEAARLR AEIDEKQAF LENVQKVQE KPEVEKKTD 
      VGTMLKCLT ITESLLLSP KVQYTPTIA TLTTEHINP 
      AMLHEDKVV QAKALKCYA LCCLIDRKC AIHGIHLFS 
      SFIITSVLA KDSEILKLS LQAVTDILI LYGTSFVEE 
      KGEENGESQ PTNTCFIGG TSLTALIEA MTNYMQDED 
      PGIEETATR CICILLLRN RLTSSSVLS ALIIKWANP 
      ATTNEGVKQ IIGHTLQSL TALPNCEYF ADAVLSTIR 
      TIVYAPGKS PLCDIDTDN ITKFMVTLC KMSSQGTKI 
      LADLALKMC NQMREKPKL KINPYFAKA LTMMDLSQS 
      ERLNSILFI CNELQEVVD KQSSKNIKK FITNASKNV 
      KLASISEE
    CDS sequence
    • >TC009926 RA
      atggaaata gaagcagtg aaagttgat gatgtcttg 
      gaaatattt aacacaatt cagcagagc tccggagcc 
      cacttacac tatgccaca atactgaaa aatgccctt 
      agtaatgaa aacgacagc gaactgttc agcacattt 
      tgcaagtgc gtcgagaag gcgttttgc ggcagttgc 
      aaggaggat atttacaat gggagaatt tttagtttt 
      gtgctcgtg ctagccgcg acatttcac ccaaaaaaa 
      gacgatgtc atacaccct gtcttaaga aagatcatt 
      cagcatctt attgctctc tcgtacttg gacaatgag 
      ccgctgcgt ttctggtcg tgtaagtta ataaatggg 
      ataatgaaa aatgtggat gaaattgac gacgagctg 
      tttgaagaa ttcaaaaac gcaatgatc gaacggctc 
      aaggaccct aaggtggct ataagaggt caagccata 
      cttgcagtg catcgccta caggaccct tcagacaaa 
      aatgacccc attactcgt cagttgtgt acttacatt 
      aattccgat tcaaatggc aaattgcgt caggtgtgt 
      gtagaaaaa gtcgcgata aataaacat gtccttact 
      ctggttttg gaaaggttg agagacatt aatttagat 
      gttagactg gcagccttt gaaaaaata cgtttactg 
      atcaaatat ttgaatttg tctcagcgg cattatatt 
      ctgcaatgt gggtttaat gatccatca caaaaagtc 
      cacgacttt gtttcaact gacttgatg aaaagttgg 
      ttggaattt tgcaataat gattacgtg aggctaatt 
      aatacaatc agattggac gctagtgaa gacgatatt 
      gaaaaaaca actatttat gctgaacag atgttgcgg 
      gcgttgttt aaaaacgca ccagttaat gacatcacg 
      tcttgtctt ttaagtcag tacttaaat gaacaaaaa 
      ttaatagaa tatgaaaaa ctcaactgg gatatcatc 
      ttgtactat cgcattgtc gtgcaattt ttgcgccag 
      tctccggaa tttgaagaa actttgaac agtattttg 
      cccgaattg gttctattt tgtaaatat atcaggggg 
      tatattgag tttattaaa acaaaacag gatttggat 
      gaattggaa tatcatttc actctaaaa caatttttt 
      attatcact gaaacatac gatgtcagt gatagcgca 
      agccgccag tgcttgaat tcactagtg catgacgcc 
      ctaaaaaaa gaagtttta ccaacaagt gtggaagaa 
      acaatcgtg cgcaatttg gagaagacg ttccctgat 
      acaaacact cgattggtt ttcgtctgc gagataata 
      tctgatatt ttttacggc gaggacgaa atgtctttt 
      gaggagttt aaaagacag gagatggag aagagccac 
      acgatttcg caattaaaa tcggacatc gccttgtta 
      tcagaacag gaaaaatac tgcgcatct gttgaaaaa 
      aattattca gaggctgca cgcctacga gccgaaatt 
      gacgagaaa caagctttc ttagagaac gtgcaaaag 
      gtgcaagaa aagccagaa gtggagaaa aagacagac 
      gtgggaaca atgctgaaa tgtttgacc atcacagaa 
      tcgcttttg ctttctccc aaagtgcag tacactccc 
      acgattgcc accttgacc accgaacac ataaacccc 
      gctatgcta catgaggac aaagtagtg caagcgaaa 
      gctctcaaa tgctacgcc ttgtgttgt ctgatagac 
      agaaaatgt gcaattcac ggcatccac ttgttttct 
      tctttcatt attacgtcc gtccttgcc aaagacagc 
      gaaattctg aagcttagc ttgcaagcc gtcactgat 
      attttgatc ttatatgga acaagtttc gttgaggaa 
      aaaggcgaa gaaaatggg gagtcccaa ccgacaaac 
      acttgcttt atcgggggc acctctttg acagcgctt 
      attgaagca atgaccaac tacatgcaa gacgaggat 
      ccgggaatt gaagagacc gctacccgt tgcatatgc 
      atattgttg ttgcgaaac cgcctcact tcatcatcc 
      gttttatcg gcacttatc ataaagtgg gcaaatccg 
      gcaacaacc aatgagggc gttaaacaa atcataggt 
      catacgttg cagtcgctt acagcttta ccaaactgc 
      gaatatttt gccgatgcg gtgttgagt acaattagg 
      actatagtc tatgctcct ggcaagagc cctttatgt 
      gatattgat actgacaat attactaaa tttatggta 
      actttatgt aaaatgtcc tctcagggc actaaaatt 
      ctagctgat ttagcgttg aagatgtgt aatcaaatg 
      cgtgaaaag ccgaaactc aaaataaat ccctatttt 
      gccaaagct ctcacaatg atggattta tcacagagt 
      gagagactg aacagtatt ttatttata tgtaacgaa 
      ttgcaagag gttgtggat aagcaaagt tcaaaaaat 
      atcaaaaag tttattaca aacgcaagt aaaaacgtt 
      aaattggcc tctatttct gaagaataa
    mRNA sequence
    • >TC009926 RA
      cttgttgtc acatttaaa attttaaac aaacggttt 
      ccccgtgtt aaaaagaaa tggaaatag aagcagtga 
      aagttgatg atgtcttgg aaatattta acacaattc 
      agcagagct ccggagccc acttacact atgccacaa 
      tactgaaaa atgccctta gtaatgaaa acgacagcg 
      aactgttca gcacatttt gcaagtgcg tcgagaagg 
      cgttttgcg gcagttgca aggaggata tttacaatg 
      ggagaattt ttagttttg tgctcgtgc tagccgcga 
      catttcacc caaaaaaag acgatgtca tacaccctg 
      tcttaagaa agatcattc agcatctta ttgctctct 
      cgtacttgg acaatgagc cgctgcgtt tctggtcgt 
      gtaagttaa taaatggga taatgaaaa atgtggatg 
      aaattgacg acgagctgt ttgaagaat tcaaaaacg 
      caatgatcg aacggctca aggacccta aggtggcta 
      taagaggtc aagccatac ttgcagtgc atcgcctac 
      aggaccctt cagacaaaa atgacccca ttactcgtc 
      agttgtgta cttacatta attccgatt caaatggca 
      aattgcgtc aggtgtgtg tagaaaaag tcgcgataa 
      ataaacatg tccttactc tggttttgg aaaggttga 
      gagacatta atttagatg ttagactgg cagcctttg 
      aaaaaatac gtttactga tcaaatatt tgaatttgt 
      ctcagcggc attatattc tgcaatgtg ggtttaatg 
      atccatcac aaaaagtcc acgactttg tttcaactg 
      acttgatga aaagttggt tggaatttt gcaataatg 
      attacgtga ggctaatta atacaatca gattggacg 
      ctagtgaag acgatattg aaaaaacaa ctatttatg 
      ctgaacaga tgttgcggg cgttgttta aaaacgcac 
      cagttaatg acatcacgt cttgtcttt taagtcagt 
      acttaaatg aacaaaaat taatagaat atgaaaaac 
      tcaactggg atatcatct tgtactatc gcattgtcg 
      tgcaatttt tgcgccagt ctccggaat ttgaagaaa 
      ctttgaaca gtattttgc ccgaattgg ttctatttt 
      gtaaatata tcagggggt atattgagt ttattaaaa 
      caaaacagg atttggatg aattggaat atcatttca 
      ctctaaaac aatttttta ttatcactg aaacatacg 
      atgtcagtg atagcgcaa gccgccagt gcttgaatt 
      cactagtgc atgacgccc taaaaaaag aagttttac 
      caacaagtg tggaagaaa caatcgtgc gcaatttgg 
      agaagacgt tccctgata caaacactc gattggttt 
      tcgtctgcg agataatat ctgatattt tttacggcg 
      aggacgaaa tgtcttttg aggagttta aaagacagg 
      agatggaga agagccaca cgatttcgc aattaaaat 
      cggacatcg ccttgttat cagaacagg aaaaatact 
      gcgcatctg ttgaaaaaa attattcag aggctgcac 
      gcctacgag ccgaaattg acgagaaac aagctttct 
      tagagaacg tgcaaaagg tgcaagaaa agccagaag 
      tggagaaaa agacagacg tgggaacaa tgctgaaat 
      gtttgacca tcacagaat cgcttttgc tttctccca 
      aagtgcagt acactccca cgattgcca ccttgacca 
      ccgaacaca taaaccccg ctatgctac atgaggaca 
      aagtagtgc aagcgaaag ctctcaaat gctacgcct 
      tgtgttgtc tgatagaca gaaaatgtg caattcacg 
      gcatccact tgttttctt ctttcatta ttacgtccg 
      tccttgcca aagacagcg aaattctga agcttagct 
      tgcaagccg tcactgata ttttgatct tatatggaa 
      caagtttcg ttgaggaaa aaggcgaag aaaatgggg 
      agtcccaac cgacaaaca cttgcttta tcgggggca 
      cctctttga cagcgctta ttgaagcaa tgaccaact 
      acatgcaag acgaggatc cgggaattg aagagaccg 
      ctacccgtt gcatatgca tattgttgt tgcgaaacc 
      gcctcactt catcatccg ttttatcgg cacttatca 
      taaagtggg caaatccgg caacaacca atgagggcg 
      ttaaacaaa tcataggtc atacgttgc agtcgctta 
      cagctttac caaactgcg aatattttg ccgatgcgg 
      tgttgagta caattagga ctatagtct atgctcctg 
      gcaagagcc ctttatgtg atattgata ctgacaata 
      ttactaaat ttatggtaa ctttatgta aaatgtcct 
      ctcagggca ctaaaattc tagctgatt tagcgttga 
      agatgtgta atcaaatgc gtgaaaagc cgaaactca 
      aaataaatc cctattttg ccaaagctc tcacaatga 
      tggatttat cacagagtg agagactga acagtattt 
      tatttatat gtaacgaat tgcaagagg ttgtggata 
      agcaaagtt caaaaaata tcaaaaagt ttattacaa 
      acgcaagta aaaacgtta aattggcct ctatttctg 
      aagaataaa taagtgcaa ttaggtgag tctgctaag 
      gtgtatttc tactatctc aaacaaaca agaataata 
      atcacaaga attcagcgt ttgcaatta agtttttat 
      atttttctc tatgcattc aaaaatccg taaaaatgt 
      caccccaaa aaataatag gataagtac aaattaata 
      ataaaattt cgattaaac ataactggt gctagcaat 
      aattttatc gaaagtttt tgctcaaaa cattattaa 
      gcttcgttg tgtccatta attcatatt tttatttta 
      ttgatatta atatttttt aaaattaat tttaattat 
      aaggaaaaa tacacatgc atagaaaaa atactttta 
      gcagactga ccccgtatt catcaatcg atagatatt 
      tattactta tttacataa ttattgaat aaaaaacta 
      agtaaacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY