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Gene information
General information
TC008987 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9222397..9227272
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008987
    iBeetle screen iB_10347

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008987 RA
      MTAFEEFGV LPEIAKAID EMEWTLPTD IQAESIPLI 
      LGGGDVLMA AETGSGKTA AFCLPIIQI VWETLKDIQ 
      SGKVSKVST SSSKPWTMS MIDRGTALA VAPCGLRVQ 
      SREQREWHG GRCTRGVQG SGKYAFEAT VTDEGLCRV 
      GWSTLQAKL DLGTCRNGF GFGGTGKKS NNKQFDDYG 
      EPFGKNDVI TCLLDLDNG QIKFCKNGT DLGNAFTIN 
      KQMLNNAFF PAVVLKNAE MAFNFGEEA FKYPLPQGY 
      VPIKQAPSE KIVVNSNSG EAADDNSVK IKNNAPQAI 
      IIEPSRELA EQTSNQIKK FKKHLESPA IRELLVIGG 
      VNVKEQIST LQNVGADII VGTPGRLED LIQGGYLLL 
      TNCRFFVLD EADGLLKQG YTELIERLH RQMPKITAD 
      GRRLQMIVC SATLHAFEV KKMAEKLMH FPTWVDLKG 
      EDAVPETVH HVVVKVDPQ KDRSWASLR RHILTDGVH 
      AQDGVRPGN NSPETLSEA VKMLKGEYC VRAIDEHQM 
      DRAIIFCRT KLDCDNLEK YFKQLDRNK YSCVCLHGD 
      RKPNERKSN LEAFKQGKV KFLICTDVA ARGLDISGL 
      PFMINVTLP DEKSNYVHR IGRVGRAER MGLAISLVS 
      SIPEKVWYH GEWCKSRGR NCWNTNLVE QKGCCIWYN 
      EPQFLADIE EHLNITIQQ IEPDMKVPH DEFDGKVVY 
      GQKRKLQTS NYENHTAQM APIVKNLSK LESEAQLLY 
      LKRHLAKLA RA
    CDS sequence
    • >TC008987 RA
      atgactgca ttcgaggaa ttcggggtt ctccccgaa 
      atcgctaag gccatcgat gagatggag tggacttta 
      ccaactgat attcaagcc gaaagcatt cccctaatt 
      ctggggggc ggtgatgtc ttgatggct gctgaaact 
      ggcagtggc aaaacggcc gcgttttgt ctacccata 
      attcagatt gtttgggag actttaaaa gatatccaa 
      agtggaaaa gtttcaaaa gtgtccact agttcatcc 
      aagccttgg acaatgtct atgattgac agagggaca 
      gctttagct gtcgcccct tgtggactg cgtgtgcag 
      tctagggaa caaagagaa tggcatggg ggacggtgt 
      actaggggg gtgcaaggg agcggtaaa tacgctttt 
      gaggccacc gtgacggat gaggggctg tgcagggtg 
      gggtggtcg actctgcag gcgaaatta gatctaggc 
      acatgcagg aacgggttt gggtttggc ggaacggga 
      aagaaatcg aacaataaa caatttgat gattacggc 
      gagcctttt ggtaaaaat gacgtaatc acgtgctta 
      ttagacctc gataatgga caaattaaa ttttgcaaa 
      aacggcacc gatttagga aatgcgttt acaatcaat 
      aaacaaatg ttaaataac gcatttttt cccgcagtt 
      gttttaaaa aacgccgaa atggctttt aacttcggg 
      gaggaagcc tttaaatac ccacttccc caggggtac 
      gtccctatc aaacaagcc ccaagcgag aaaattgta 
      gtcaattcg aattctggt gaagcggcc gatgataat 
      tcagttaaa ataaaaaat aacgcccct caagcaata 
      ataatcgaa ccgtcaaga gaactggct gagcaaact 
      tcaaaccag ataaaaaaa ttcaaaaaa catttggaa 
      tctcccgca attcgcgaa cttctagtc attggaggc 
      gtcaatgtg aaggaacaa atctcgacg ttacaaaac 
      gtgggggct gatatcatt gttggcacc cctggccgt 
      ttggaggac ttgatccag gggggttat ttattgctc 
      accaactgt cgatttttt gtcttggac gaggctgac 
      ggtctttta aagcaaggc tacacagaa ctgattgaa 
      agactccac cgacaaatg ccgaaaata accgccgat 
      ggccgaagg ctgcaaatg atcgtttgc agcgccacc 
      ttgcacgcg tttgaggtg aagaaaatg gctgaaaaa 
      ctgatgcat ttcccgacg tgggtcgac ttgaaaggc 
      gaggacgcg gttcctgag actgtacat cacgtggtg 
      gttaaggtg gacccccag aaggacagg tcttgggcg 
      agtctccgc agacacatt ttgacagac ggcgttcat 
      gcgcaagat ggcgtcaga ccggggaat aacagcccg 
      gagacgctc agtgaggcg gtgaaaatg ctcaaaggg 
      gaatattgc gtacgagct atagatgaa catcagatg 
      gacagggcg attattttc tgtcgcacg aaactggat 
      tgtgataac ttggaaaaa tatttcaag caacttgat 
      cgtaataag tactcgtgt gtgtgtttg cacggagac 
      cggaaacca aacgaacgc aagagcaat ttggaggct 
      ttcaagcag gggaaagtg aagtttttg atttgcact 
      gatgtcgcg gcgagggga ctcgatatt tccggtttg 
      ccttttatg attaatgtt actttaccg gacgagaag 
      tcgaattat gtgcatagg atcgggagg gttggcaga 
      gccgagcga atgggcctt gcgataagt ctagtgagc 
      tcaattccg gaaaaagtg tggtaccac ggcgagtgg 
      tgcaagagc agagggcgc aactgttgg aacaccaac 
      ctagtggag caaaaaggt tgttgcatt tggtacaac 
      gagccccag tttttggcc gacattgaa gagcatttg 
      aacattaca attcaacaa atcgaaccc gatatgaaa 
      gtgccgcat gatgagttt gacggaaaa gtagtttac 
      ggccaaaag aggaagctc cagaccagt aattacgaa 
      aaccacacc gcccagatg gcacccata gttaaaaat 
      ttgtcaaag ttggagagc gaagctcag ttgttgtat 
      ctgaaaaga cacttggca aagttggca agggcttga
    mRNA sequence
    • >TC008987 RA
      tgtcaaacg ctgtcaaaa gaagttgac cggtgaaat 
      ttttatttt tttggaaaa atataaaaa aatagcttg 
      acacaactg aatatttca cgtttttta taatcctcg 
      gttatattt cattgcaaa caatgactg cattcgagg 
      aattcgggg ttctccccg aaatcgcta aggccatcg 
      atgagatgg agtggactt taccaactg atattcaag 
      ccgaaagca ttcccctaa ttctggggg gcggtgatg 
      tcttgatgg ctgctgaaa ctggcagtg gcaaaacgg 
      ccgcgtttt gtctaccca taattcaga ttgtttggg 
      agactttaa aagatatcc aaagtggaa aagtttcaa 
      aagtgtcca ctagttcat ccaagcctt ggacaatgt 
      ctatgattg acagaggga cagctttag ctgtcgccc 
      cttgtggac tgcgtgtgc agtctaggg aacaaagag 
      aatggcatg ggggacggt gtactaggg gggtgcaag 
      ggagcggta aatacgctt ttgaggcca ccgtgacgg 
      atgaggggc tgtgcaggg tggggtggt cgactctgc 
      aggcgaaat tagatctag gcacatgca ggaacgggt 
      ttgggtttg gcggaacgg gaaagaaat cgaacaata 
      aacaatttg atgattacg gcgagcctt ttggtaaaa 
      atgacgtaa tcacgtgct tattagacc tcgataatg 
      gacaaatta aattttgca aaaacggca ccgatttag 
      gaaatgcgt ttacaatca ataaacaaa tgttaaata 
      acgcatttt ttcccgcag ttgttttaa aaaacgccg 
      aaatggctt ttaacttcg gggaggaag cctttaaat 
      acccacttc cccaggggt acgtcccta tcaaacaag 
      ccccaagcg agaaaattg tagtcaatt cgaattctg 
      gtgaagcgg ccgatgata attcagtta aaataaaaa 
      ataacgccc ctcaagcaa taataatcg aaccgtcaa 
      gagaactgg ctgagcaaa cttcaaacc agataaaaa 
      aattcaaaa aacatttgg aatctcccg caattcgcg 
      aacttctag tcattggag gcgtcaatg tgaaggaac 
      aaatctcga cgttacaaa acgtggggg ctgatatca 
      ttgttggca cccctggcc gtttggagg acttgatcc 
      aggggggtt atttattgc tcaccaact gtcgatttt 
      ttgtcttgg acgaggctg acggtcttt taaagcaag 
      gctacacag aactgattg aaagactcc accgacaaa 
      tgccgaaaa taaccgccg atggccgaa ggctgcaaa 
      tgatcgttt gcagcgcca ccttgcacg cgtttgagg 
      tgaagaaaa tggctgaaa aactgatgc atttcccga 
      cgtgggtcg acttgaaag gcgaggacg cggttcctg 
      agactgtac atcacgtgg tggttaagg tggaccccc 
      agaaggaca ggtcttggg cgagtctcc gcagacaca 
      ttttgacag acggcgttc atgcgcaag atggcgtca 
      gaccgggga ataacagcc cggagacgc tcagtgagg 
      cggtgaaaa tgctcaaag gggaatatt gcgtacgag 
      ctatagatg aacatcaga tggacaggg cgattattt 
      tctgtcgca cgaaactgg attgtgata acttggaaa 
      aatatttca agcaacttg atcgtaata agtactcgt 
      gtgtgtgtt tgcacggag accggaaac caaacgaac 
      gcaagagca atttggagg ctttcaagc aggggaaag 
      tgaagtttt tgatttgca ctgatgtcg cggcgaggg 
      gactcgata tttccggtt tgcctttta tgattaatg 
      ttactttac cggacgaga agtcgaatt atgtgcata 
      ggatcggga gggttggca gagccgagc gaatgggcc 
      ttgcgataa gtctagtga gctcaattc cggaaaaag 
      tgtggtacc acggcgagt ggtgcaaga gcagagggc 
      gcaactgtt ggaacacca acctagtgg agcaaaaag 
      gttgttgca tttggtaca acgagcccc agtttttgg 
      ccgacattg aagagcatt tgaacatta caattcaac 
      aaatcgaac ccgatatga aagtgccgc atgatgagt 
      ttgacggaa aagtagttt acggccaaa agaggaagc 
      tccagacca gtaattacg aaaaccaca ccgcccaga 
      tggcaccca tagttaaaa atttgtcaa agttggaga 
      gcgaagctc agttgttgt atctgaaaa gacacttgg 
      caaagttgg caagggctt gaaacacag tgttgaggt 
      aataatgcg tacatcaat ttatttgag ataaacaat 
      atataaaat acaatagtt taaacaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY