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Gene information
General information
TC009845 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8982297..8984700
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009845
    iBeetle screen iB_10346

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009845 RA
      MEGNKLELS EKPSADPPA TYKAIPEAD MEEDAVTRP 
      KSLVKPNAA SPEADGADE KMLPKEEVA DAKISPNEK 
      QNGDAKLDI GDLKTAFVG LTKEELMKY ANDPFWVRL 
      RWFLFITFW ILWGLMLLG AVMIILAAP KCNPPPPRT 
      WWEKGPLVE LKDDASEET IKKVKELGV TGVIISWPE 
      NAYSEFSED HELIKSFKQ FKEKEINVV VELEPSASP 
      LVWFNKSES RDPLFSEFY IWRQPKEAS NGGEPTPPN 
      NWLSVRNVS SWKYSPNRK EFYYAPMDK PHLNFYNPR 
      VVEKFSEVI KKFLNYGAG GIRLKGAPY LLVDPNFED 
      EPIKIPNPT GDGFKEHSF YTHPKTKNV MQLGPLLKQ 
      WRFIVKNMT ENGPFMVAE HLSNIEAYK VNSSFVVDL 
      PLQSHLLNK PSVNITDVV HVLNTTFNA DNITWPLWK 
      VNASSEWDL VTYLLPGTP LVMLESENN KELLKIRNS 
      PSIMRGSFS SHLLNNKTV FAFTRVTAG NPGILVALN 
      PSDNRTVVD FPAHIKGLS EDVTVQLFS ANYSEPNVA 
      VSVKHDAHS VPISPKSAI VMSYVPPSK
    CDS sequence
    • >TC009845 RA
      atggaaggc aacaagttg gagctatca gagaagcca 
      agcgccgac ccgccggcc acttacaag gcaatacct 
      gaagctgac atggaagag gacgccgtt actcgaccc 
      aaaagccta gtgaaaccg aacgcagcc agtccggag 
      gccgacggc gcggacgag aagatgctg ccgaaggag 
      gaagtggcc gatgctaag atttcgccc aacgagaag 
      caaaacggc gatgcgaaa ctggacatt ggcgacctc 
      aagaccgcg tttgtgggc ctcaccaaa gaggagttg 
      atgaagtac gccaacgac ccgttttgg gtgcgtttg 
      cgctggttt ttgttcatc acgttttgg atcttgtgg 
      gggttgatg cttctgggg gctgttatg atcattctg 
      gccgctcct aagtgtaac cccccacca ccgaggact 
      tggtgggag aaaggtcct ctagttgag ctgaaggat 
      gatgcaagt gaagaaaca atcaagaaa gttaaggaa 
      ttgggagtt acaggagtt ataatttca tggcctgaa 
      aatgcttat tctgagttt agtgaagat catgaattg 
      atcaagtca ttcaagcag ttcaaggaa aaggaaata 
      aatgtggtt gtggaactt gaacctagt gcatcccct 
      ttggtttgg ttcaataaa tctgaaagt agggatcct 
      ctattcagt gaattttat atttggcgt caacctaaa 
      gaggctagt aatggaggg gagccaaca cccccaaat 
      aactggctc tcagtaagg aatgtctca tcgtggaaa 
      tactctcca aatcgcaaa gaattttat tatgcacca 
      atggataag ccccacctt aatttctac aaccctaga 
      gttgttgaa aaattctct gaagttatt aaaaagttc 
      ctaaattat ggcgcaggc gggattagg cttaaagga 
      gctccctac ctattggtg gatccaaac tttgaagac 
      gaaccaatt aaaatccca aatcccact ggagatggt 
      ttcaaagaa cacagtttt tacacgcac ccaaagact 
      aaaaatgtg atgcaattg gggccacta ttaaaacag 
      tggagattt attgttaaa aacatgaca gaaaatggg 
      ccatttatg gtagctgaa catttaagc aatatcgaa 
      gcttacaag gtcaacagc tcatttgtt gttgatctg 
      cctcttcaa tcgcattta ttgaataaa ccaagtgtt 
      aacatcacc gatgttgtt cacgtttta aatacgaca 
      tttaatgct gataatatc acttggccg ttatggaaa 
      gtcaacgct tcgagtgag tgggatctt gtcacttat 
      ttgttaccc gggacgcca ctggtgatg ttggaatcg 
      gaaaacaac aaggagttg ttgaagata cgaaattca 
      ccttctatt atgagaggt tccttctcc tcgcattta 
      ctcaacaat aagactgtg tttgcattt acaagggta 
      actgctggt aaccctgga atattggtc gccttaaac 
      ccctcggac aatcgcacg gtggtcgac ttccctgcc 
      catatcaaa ggtctgtca gaagatgtg actgttcag 
      ctgtttagc gctaattac agcgaacca aatgtagca 
      gtcagtgta aaacatgac gcacattct gtgcctatt 
      tcgccaaaa tcagcaatt gtaatgtca tatgtaccg 
      ccatctaaa taa
    mRNA sequence
    • >TC009845 RA
      aattatcat aaagtaaaa atgagtaag ttcacctaa 
      atttacaac gaaagtgcg tcggaagcg gcgccaggt 
      gtttaaaca aatagggaa ttccctatt ttccacaat 
      agttggtat cacaattga tgcctccaa aaaccagtc 
      acatgtcat ttgaattcg gtggagttt ggtcacgtc 
      agtggctgg ctgttatcg attgacccc aagggcgta 
      ttccctgct ccattattt gcccaatta ggccctaat 
      tgctccata atggaaggc aacaagttg gagctatca 
      gagaagcca agcgccgac ccgccggcc acttacaag 
      gcaatacct gaagctgac atggaagag gacgccgtt 
      actcgaccc aaaagccta gtgaaaccg aacgcagcc 
      agtccggag gccgacggc gcggacgag aagatgctg 
      ccgaaggag gaagtggcc gatgctaag atttcgccc 
      aacgagaag caaaacggc gatgcgaaa ctggacatt 
      ggcgacctc aagaccgcg tttgtgggc ctcaccaaa 
      gaggagttg atgaagtac gccaacgac ccgttttgg 
      gtgcgtttg cgctggttt ttgttcatc acgttttgg 
      atcttgtgg gggttgatg cttctgggg gctgttatg 
      atcattctg gccgctcct aagtgtaac cccccacca 
      ccgaggact tggtgggag aaaggtcct ctagttgag 
      ctgaaggat gatgcaagt gaagaaaca atcaagaaa 
      gttaaggaa ttgggagtt acaggagtt ataatttca 
      tggcctgaa aatgcttat tctgagttt agtgaagat 
      catgaattg atcaagtca ttcaagcag ttcaaggaa 
      aaggaaata aatgtggtt gtggaactt gaacctagt 
      gcatcccct ttggtttgg ttcaataaa tctgaaagt 
      agggatcct ctattcagt gaattttat atttggcgt 
      caacctaaa gaggctagt aatggaggg gagccaaca 
      cccccaaat aactggctc tcagtaagg aatgtctca 
      tcgtggaaa tactctcca aatcgcaaa gaattttat 
      tatgcacca atggataag ccccacctt aatttctac 
      aaccctaga gttgttgaa aaattctct gaagttatt 
      aaaaagttc ctaaattat ggcgcaggc gggattagg 
      cttaaagga gctccctac ctattggtg gatccaaac 
      tttgaagac gaaccaatt aaaatccca aatcccact 
      ggagatggt ttcaaagaa cacagtttt tacacgcac 
      ccaaagact aaaaatgtg atgcaattg gggccacta 
      ttaaaacag tggagattt attgttaaa aacatgaca 
      gaaaatggg ccatttatg gtagctgaa catttaagc 
      aatatcgaa gcttacaag gtcaacagc tcatttgtt 
      gttgatctg cctcttcaa tcgcattta ttgaataaa 
      ccaagtgtt aacatcacc gatgttgtt cacgtttta 
      aatacgaca tttaatgct gataatatc acttggccg 
      ttatggaaa gtcaacgct tcgagtgag tgggatctt 
      gtcacttat ttgttaccc gggacgcca ctggtgatg 
      ttggaatcg gaaaacaac aaggagttg ttgaagata 
      cgaaattca ccttctatt atgagaggt tccttctcc 
      tcgcattta ctcaacaat aagactgtg tttgcattt 
      acaagggta actgctggt aaccctgga atattggtc 
      gccttaaac ccctcggac aatcgcacg gtggtcgac 
      ttccctgcc catatcaaa ggtctgtca gaagatgtg 
      actgttcag ctgtttagc gctaattac agcgaacca 
      aatgtagca gtcagtgta aaacatgac gcacattct 
      gtgcctatt tcgccaaaa tcagcaatt gtaatgtca 
      tatgtaccg ccatctaaa taaacctgc aattatttg 
      aatataaag gcattccat tgtcttgct tttatactt 
      tgtgttgaa cagtaaata tatttcaaa acgcagc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY