Status
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Gene information
General information
TC033711
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030623,TC030719
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8936097..8939281
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033711
    iBeetle screen iB_10345

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033711 RA
      MLALRTASS LIRVVNFRA PKTKVRTLL SPNIRYISK 
      TTIFFNNKN ETLPVRIVG SAPKSVQPY LKLMRLDKP 
      IGTWLLFWP CGWGIASAA APGCFPDPY MLALFGTGA 
      VIMRGAGCT INDMWDRDI DSKVARTKD RPLVNGDVS 
      MKAAWVFLA GQLSLGLAV LLQLNWYSV FLGASSMGL 
      VISYPLMKR FTYWPQLVL GLTFNWGVL LGYSAIKGH 
      VDLPVCLPL YLASVCWTI IYDTIYAHQ DRADDYKLG 
      IKSTALKFD RDTKLWLSG FSAVMMGGV VLSGIMNEM 
      AWPYYASTA LVASHLASQ LFTLNIDNA SDCSNKFVS 
      NAQVGLILF CGIVLGNYF KKDKGKKEN FHSANMDTV 
      KHLVSRGIC LIVWPILLY ITFFYIHLS VLNRSGNGD 
      GFYSSAFQS QLIGNSLHN ASMPRQVAY GAIITLKNH 
      RTGGGYLHS HYHLYPENV GARQQQITT YTHKDDNNK 
      WLVKKYNTE DTSGVTIVR SGDLVRLEH VPTRRNLHS 
      HKEQAPITK KHYQVTGYG ENGTGDAND VWKITVIGA 
      KDGSEVTAV SSKLKFVHY LQSCILTTS GKQLPKWAY 
      EQQEVSCNP NLRDPHGVW NVEENIFEK LPNVSFEVY 
      APSFLERFF ESHAVMFQG NAGLKPKEG EITSRPWQW 
      PINYRGQFF SGSSYRVYL LGNPVIWWA NIIFLLVFV 
      FVFGANAIR QQRGYIKSF SESHKEKLV ACAWLFLGW 
      LLHYVPFWA MGRVLYFHH YFPALLFSS MITGIIIDY 
      LLEEIPKFF TEKTGNVVY HTLVALVLS TVVYSFYLF 
      APLTYGMSG PNANEPNST MYGLKWLDT WEF
    CDS sequence
    • >TC033711 RA
      atgttagct ctcagaacc gcaagttcg ttgatcagg 
      gtggttaat ttccgcgca ccgaaaact aaggttagg 
      acactgtta agtccaaat atccgctac atttcaaag 
      acgacaatc tttttcaac aacaagaat gagaccctc 
      cctgtgcga atcgtcggt agtgcccct aagtcagtg 
      caaccctac ttgaaacta atgcgactt gacaaacca 
      atcggcaca tggttgctg ttctggccc tgtggctgg 
      ggcatagcc tcggcggcc gcgcctggg tgtttcccc 
      gatccctac atgctggcc ttatttgga acgggggct 
      gtaatcatg cggggcgcc ggctgcaca atcaacgat 
      atgtgggac cgcgatatc gattcaaag gtcgcacga 
      accaaagac cggccttta gtcaacggc gatgtttct 
      atgaaagcc gcttgggtt tttcttgcg ggtcagtta 
      agcctgggt ttggccgtt ttgttacaa ttaaactgg 
      tatagtgtg ttcctggga gcaagttcc atgggtcta 
      gtcataagt tatccgcta atgaagcgc ttcacctat 
      tggccccag ttggtgcta gggctgacg ttcaactgg 
      ggcgtcctc ttggggtac agtgccatc aaggggcac 
      gtcgatttg cctgtttgc ttgccattg tatttagct 
      tcggtgtgt tggactata atttacgac acgatttat 
      gcgcatcaa gaccgggct gatgattat aagttgggg 
      attaagtcg acggcgctt aagtttgat agggatacg 
      aagttgtgg ttgagtgga tttagtgct gtgatgatg 
      ggaggggtt gtgctctca gggattatg aatgagatg 
      gcgtggcca tactacgcc tccactgct ttagtggcc 
      tcacatctt gcctcccag ttatttaca ctcaacatt 
      gacaatgcc agtgattgt tcgaataaa ttcgtgtcg 
      aatgcgcaa gtcggattg atcctgttc tgtggaata 
      gtcttagga aattatttc aaaaaagat aaaggcaaa 
      aaggaaaat tttcattcc gccaatatg gacacagtt 
      aagcattta gtatcaaga ggaatttgc ctgattgtt 
      tggccaatt ctcctttac ataactttc ttctacata 
      catttgtca gtattaaac agaagcgga aacggcgat 
      ggcttctac agttcagcg tttcaatcc cagttaata 
      gggaatagt cttcacaat gccagtatg ccgcgccaa 
      gtggcttac ggggctata atcactcta aaaaaccac 
      cgaacgggt gggggttac ctacactcc cattaccac 
      ctgtatccg gaaaatgtt ggagcccga cagcaacag 
      atcaccact tacacccac aaagacgat aacaacaag 
      tggttggtc aaaaaatac aacactgaa gacacttcc 
      ggcgttaca atcgttcgc agcggcgac ttagtccgt 
      ctagaacac gtcccaact cgacgcaac ctccattcc 
      cacaaagag caagccccc atcactaaa aaacactac 
      caagtgacc ggttatggc gaaaacggg accggggac 
      gcgaacgac gtctggaaa atcaccgtt atcggagcc 
      aaagacggc agtgaagtc actgcagtc agcagcaaa 
      ttgaaattc gtccattat ttacaatcc tgtattctc 
      acaactagc ggaaaacag ctcccgaaa tgggcgtac 
      gagcaacag gaggtttca tgcaacccc aatctcaga 
      gacccccac ggagtgtgg aacgtcgag gagaatatt 
      ttcgaaaaa ttgccaaat gtgagtttc gaggtgtat 
      gcgcccagt tttctggag cggtttttc gagtcccac 
      gctgtgatg ttccaagga aacgctggg ttgaaaccg 
      aaggaaggg gagatcacg tcgaggccg tggcagtgg 
      cctattaat tacagaggc caattcttt tccggttca 
      agctacaga gtgtattta ttaggaaat ccggtcatt 
      tggtgggcc aatatcatt ttcctacta gttttcgtg 
      tttgttttt ggggccaat gccattcgg caacaacgg 
      ggctacatt aagtcgttt tccgagtcc cacaaagag 
      aaattggtg gcgtgtgcg tggctgttt ttggggtgg 
      cttttgcat tatgtgccg ttttgggca atgggccgc 
      gttttgtat ttccaccat tatttcccg gctttgctt 
      tttagttcg atgattaca ggaatcatc attgattat 
      ttattggag gagataccg aaatttttc acagaaaag 
      acaggcaac gttgtgtat catacattg gtggcgtta 
      gttttgtcc acagtggtt tatagcttt tatttattc 
      gcgccgtta acatacgga atgagcggt cctaatgcc 
      aacgagccc aattctaca atgtatggg ctgaaatgg 
      ttggacacg tgggaattt taa
    mRNA sequence
    • >TC033711 RA
      ttgcaaaaa tcgcatttt aatgaaaat atgatgcta 
      acacaataa attgacaat aaaattgga cgcgtaccg 
      tcgttggcg cataatgtt agctctcag aaccgcaag 
      ttcgttgat cagggtggt taatttccg cgcaccgaa 
      aactaaggt taggacact gttaagtcc aaatatccg 
      ctacatttc aaagacgac aatcttttt caacaacaa 
      gaatgagac cctccctgt gcgaatcgt cggtagtgc 
      ccctaagtc agtgcaacc ctacttgaa actaatgcg 
      acttgacaa accaatcgg cacatggtt gctgttctg 
      gccctgtgg ctggggcat agcctcggc ggccgcgcc 
      tgggtgttt ccccgatcc ctacatgct ggccttatt 
      tggaacggg ggctgtaat catgcgggg cgccggctg 
      cacaatcaa cgatatgtg ggaccgcga tatcgattc 
      aaaggtcgc acgaaccaa agaccggcc tttagtcaa 
      cggcgatgt ttctatgaa agccgcttg ggtttttct 
      tgcgggtca gttaagcct gggtttggc cgttttgtt 
      acaattaaa ctggtatag tgtgttcct gggagcaag 
      ttccatggg tctagtcat aagttatcc gctaatgaa 
      gcgcttcac ctattggcc ccagttggt gctagggct 
      gacgttcaa ctggggcgt cctcttggg gtacagtgc 
      catcaaggg gcacgtcga tttgcctgt ttgcttgcc 
      attgtattt agcttcggt gtgttggac tataattta 
      cgacacgat ttatgcgca tcaagaccg ggctgatga 
      ttataagtt ggggattaa gtcgacggc gcttaagtt 
      tgataggga tacgaagtt gtggttgag tggatttag 
      tgctgtgat gatgggagg ggttgtgct ctcagggat 
      tatgaatga gatggcgtg gccatacta cgcctccac 
      tgctttagt ggcctcaca tcttgcctc ccagttatt 
      tacactcaa cattgacaa tgccagtga ttgttcgaa 
      taaattcgt gtcgaatgc gcaagtcgg attgatcct 
      gttctgtgg aatagtctt aggaaatta tttcaaaaa 
      agataaagg caaaaagga aaattttca ttccgccaa 
      tatggacac agttaagca tttagtatc aagaggaat 
      ttgcctgat tgtttggcc aattctcct ttacataac 
      tttcttcta catacattt gtcagtatt aaacagaag 
      cggaaacgg cgatggctt ctacagttc agcgtttca 
      atcccagtt aatagggaa tagtcttca caatgccag 
      tatgccgcg ccaagtggc ttacggggc tataatcac 
      tctaaaaaa ccaccgaac gggtggggg ttacctaca 
      ctcccatta ccacctgta tccggaaaa tgttggagc 
      ccgacagca acagatcac cacttacac ccacaaaga 
      cgataacaa caagtggtt ggtcaaaaa atacaacac 
      tgaagacac ttccggcgt tacaatcgt tcgcagcgg 
      cgacttagt ccgtctaga acacgtccc aactcgacg 
      caacctcca ttcccacaa agagcaagc ccccatcac 
      taaaaaaca ctaccaagt gaccggtta tggcgaaaa 
      cgggaccgg ggacgcgaa cgacgtctg gaaaatcac 
      cgttatcgg agccaaaga cggcagtga agtcactgc 
      agtcagcag caaattgaa attcgtcca ttatttaca 
      atcctgtat tctcacaac tagcggaaa acagctccc 
      gaaatgggc gtacgagca acaggaggt ttcatgcaa 
      ccccaatct cagagaccc ccacggagt gtggaacgt 
      cgaggagaa tattttcga aaaattgcc aaatgtgag 
      tttcgaggt gtatgcgcc cagttttct ggagcggtt 
      tttcgagtc ccacgctgt gatgttcca aggaaacgc 
      tgggttgaa accgaagga aggggagat cacgtcgag 
      gccgtggca gtggcctat taattacag aggccaatt 
      cttttccgg ttcaagcta cagagtgta tttattagg 
      aaatccggt catttggtg ggccaatat cattttcct 
      actagtttt cgtgtttgt ttttggggc caatgccat 
      tcggcaaca acggggcta cattaagtc gttttccga 
      gtcccacaa agagaaatt ggtggcgtg tgcgtggct 
      gtttttggg gtggctttt gcattatgt gccgttttg 
      ggcaatggg ccgcgtttt gtatttcca ccattattt 
      cccggcttt gctttttag ttcgatgat tacaggaat 
      catcattga ttatttatt ggaggagat accgaaatt 
      tttcacaga aaagacagg caacgttgt gtatcatac 
      attggtggc gttagtttt gtccacagt ggtttatag 
      cttttattt attcgcgcc gttaacata cggaatgag 
      cggtcctaa tgccaacga gcccaattc tacaatgta 
      tgggctgaa atggttgga cacgtggga attttaacg 
      attttgtac tcaattttg tattctttt caatattaa 
      aaattgttc attaatcgt taataatta ttttaagct 
      tctcaaaca atacactaa taattcaga gacgtccgt 
      cccagctat tatttatta tttcaattt gtagtagag 
      ataaaccgt ttcagagaa taatgtttg agagataag 
      actcgtacg aacagtttt aatttcatt ttcgtgcca 
      tatctttga aatctaacc agcaggtaa ttataaaaa 
      gttgtaaaa caattattt ttaagttaa tgtagataa 
      ttaaacttt tctgaaaca agtgttaaa gccagaact 
      gttctatca gaattcgga taaggcaat aaacactga 
      taacaaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY