Status
Please wait ...
Gene information
General information
TC033703 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8618096..8620060
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033703
    iBeetle screen iB_10344

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033703 RA
      MKFRGLITD TSVMKDFMN IATGLSRFS KECVMRITE 
      RKVYFIISD EDNGPRRPL AWCELPVSF YFREYNLVG 
      VTEEHNEIY LEFSTALLA RSLSILKQN VKSLKIKLT 
      NKDSPCLTL EMEMASEAT QSRQCVHDI PVEVISRKH 
      WNDYEEPRF NDFHVSIEM PNLKTIKTI VERMRNMSH 
      SLTVAATKD GRLTFQIKT NIVTLSSHF PGLGVESFA 
      IGQLELTSS DSQEEDQQK VSSIVDIKK FLMFLSGMQ 
      LNSCRTVCS IVHGKMVKL FMEQPGALS LQIFLTQLS 
      I
    CDS sequence
    • >TC033703 RA
      atgaaattt cgtggtctc ataacagac acttcagta 
      atgaaggat tttatgaac atagctaca ggcctttcc 
      agattttca aaagaatgt gtaatgcga ataacagaa 
      agaaaagtg tatttcata atatcagat gaagataac 
      ggacctaga aggccacta gcgtggtgc gaattacct 
      gttagtttt tattttaga gaatataac ttggttggg 
      gttacagaa gaacataat gagatttat ttagagttt 
      tcgacagca ctcttggca aggtcatta tcaattctt 
      aagcaaaat gttaagtca ctaaaaatt aaattaacc 
      aataaagat tctccttgt ctaacactt gaaatggaa 
      atggcgtct gaggctaca caaagcaga caatgtgtg 
      catgatatt ccagttgag gtgatttct aggaaacat 
      tggaatgat tatgaggaa ccaagattt aatgatttt 
      cacgtttca atagaaatg cctaattta aaaacgatt 
      aaaacaata gttgaaaga atgaggaat atgagtcac 
      agtttaact gtagcagct acaaaggat ggacgactt 
      acatttcaa ataaaaacg aacattgtt acgctatca 
      tcacatttt cccggtcta ggagttgaa agttttgcc 
      attggacaa ttggaattg acttcatca gattcacaa 
      gaagaggac cagcagaaa gttagctca atagtggac 
      atcaaaaag ttcctaatg tttttatca ggaatgcag 
      ctaaacagt tgccgaaca gtctgcagc atagtccat 
      ggtaaaatg gtaaagctc ttcatggaa cagcctggg 
      gctctctcc ttacaaatt tttctcacc caattaagt 
      atttaa
    mRNA sequence
    • >TC033703 RA
      ccatttaaa aatttatta caaattaca aaaagtgag 
      gttataaca aatagtcat aaaaacaga gttctaaaa 
      attagtaaa taatccatt atgaaattt cgtggtctc 
      ataacagac acttcagta atgaaggat tttatgaac 
      atagctaca ggcctttcc agattttca aaagaatgt 
      gtaatgcga ataacagaa agaaaagtg tatttcata 
      atatcagat gaagataac ggacctaga aggccacta 
      gcgtggtgc gaattacct gttagtttt tattttaga 
      gaatataac ttggttggg gttacagaa gaacataat 
      gagatttat ttagagttt tcgacagca ctcttggca 
      aggtcatta tcaattctt aagcaaaat gttaagtca 
      ctaaaaatt aaattaacc aataaagat tctccttgt 
      ctaacactt gaaatggaa atggcgtct gaggctaca 
      caaagcaga caatgtgtg catgatatt ccagttgag 
      gtgatttct aggaaacat tggaatgat tatgaggaa 
      ccaagattt aatgatttt cacgtttca atagaaatg 
      cctaattta aaaacgatt aaaacaata gttgaaaga 
      atgaggaat atgagtcac agtttaact gtagcagct 
      acaaaggat ggacgactt acatttcaa ataaaaacg 
      aacattgtt acgctatca tcacatttt cccggtcta 
      ggagttgaa agttttgcc attggacaa ttggaattg 
      acttcatca gattcacaa gaagaggac cagcagaaa 
      gttagctca atagtggac atcaaaaag ttcctaatg 
      tttttatca ggaatgcag ctaaacagt tgccgaaca 
      gtctgcagc atagtccat ggtaaaatg gtaaagctc 
      ttcatggaa cagcctggg gctctctcc ttacaaatt 
      tttctcacc caattaagt atttaaacc atttatttt 
      gtaaaaacg cacgcacac actattaaa gtaaccaga 
      atcaaaaac ggcagccat gtcttcaca aacccagca 
      aaactaaca tactttgag tacaacaac ataaaaaat 
      gccaatttc accatattc caacttttc gaatcatca 
      tattgcatc agattaagc gcggtggca acgtgagag 
      tgacaatta tcgcagcac aaattgtgc atacgtccg 
      ctataaatt tccgaagct tcagtaact gcacggtcc 
      cagccggaa attcccccg tgtgccagt gacggtttc 
      agctgccag tatttagta gggcggccg aaggccatg 
      tcgtcttcg gaaacatag taagggccg gcaaaatcc 
      cggataacc cccgaggac agggctatc cccatgtgc 
      ccaataatg gggaaaagc caggtcagg aacgggatt 
      ggggtccag actatgcag caagggtag cggtcgcgg 
      gacttgtcg atttcgccg aactgtgcc atgtcgcca 
      ctttcaggg cttgccatg tcattaagc cgcaaaatt 
      ttggatttt tagccccac tcgtgacct aacctataa 
      accagtgtt tatctgtca aatctgtga ataatacag 
      acgatcagc tggttcttg ttaatgctt acactacaa 
      agttattga cgattaatt tgactcact gcattttta 
      gaaggtaac tacgcacga attgcaagt gcttgttat 
      tagaagagg cgcataaat ttaaactca tac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY