| General information | |||
| TC009817 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 8554615..8575202 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009817 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_10343 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
larval musculature pattern irregular -
Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva cuticle dorsal not fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva cuticle dorsal not fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC009817 RA
MCDACSDFV QLLVSYEAQ VCRESNEQP TLTKHEIDV IYVYVQTWP ARQCMCCYR DSKNLERFS YITQGLIYM VVCQLKQLA EKGSDAKKE ENKEAADKE NEVQDRDKL LILVSKVFL LNFPLYVAY KHTIQAKLD ELTQQEMQN LNLFCDLHD SEIPIYLLK NVSWFCKMG GLLAMTACF TQLAPEQLP VSTAHAMIS IVSNLKLWM NYKSMVSML VPLRSCVLR YMCKLSDQD LRAPTTKSM ADFMWNAIK DPLDTPVAF DIDGLDLAF KYFTSSTLT MRLAGITQI NNQIGVLAE ICVGESLPE AEAAPRHMA DWLINNNII SHIFGPNLH VEVIKQSHI ILNFLAMEG RITCEHMDI IWAAAQLKH CSKPVHDLL PGLVKHLAA SPTLHLYSL LTKLEPKEH TEQTLYLAS ALIKAVWSR GGGVPEVGL INVAASNAG ITLHKCATS SENSVSIDP SNSEEEHGE STGQSESHK SDSEDIDGV EIDEDDGPI DGGNPGPPP CKLARKQAI ARVHCDGSS TDPEIDGIP IKSPAEKPK RCAVVPKVK AEKRQMSSV RKKILSTLN VLPEGIEAI GSSSSQDEA DVEEVPAKF RKRRKMLRK TRVKRQKGV HKRVVAISG IEALSEVEV ESDSEMKAA LKGVAAAAL VLPAQMSGL VETNLKPAD YLDESSSCS ELGKDSVTL ESHEAACTN FLTLHSRGG HILEMLSGE EAEVEGDAD GSYSSRMSN KSEKNMADF DGEDSVCEE ELAQLNEHA QLNLHALAN QSPEKSPVR VDPRLSACF KADNVCQPG NTLLWDLLQ DDKICHLGE GLALEAEKT LCNLICYTP DKEIRMKFI EGCLHNLAN NVSVVVSLR MLPKLLTSF RGIDMHHVT HWAERQHHM MKHFFNNLK TYTANPSKS LPLYTHQMQ VQVRLHFLS AVFSPVVSP NTFKLSIEQ VDTLWECLA HDPECSDEL FSWLLSQTK TTELHALKI DALRRLYMH HLPSLPPEK FSMICLSLF QQLCSLNHL LTINMGSES VRESYNVGM DHMWKIALR ANNTDVSMA AIQYINSYY MGQNLQNEG QFVWQCMTH LKAATEDLV SVSTPGAEE APLLCIQRA LLLMKSHLE TFRRRYAYH LRRWTLEGK GIGSHSASS VDRCGSPLR LIVQSGSST ERCYLDMLS SDYVADLRA EIVKWSEGP SHTKNQESE SSHKTNDTW IRLITQGQE LTIDCDEKT LAEMGFKDN QMVYISIGV SKNMKKREC LDLPSMQPP PPRESIPTL LLLRPNYFE QLFSLMHTL SSMKVPVKG GHHIPNTKA QVLSRRVWD ILSLLPTNP TLLQGFKQL DTPLTELLD PSSAQKLMY SLYIVESLS AKISKPGEN DKESWSRTF IQHGGLRHL FDIFMSGVL QRDGGNGSE WQQDCLASL LKIICQLGV ESLPIESRP VRSDKIVIP NLNEAMLAM IDVKNTMPQ LTSILEEAS LPKDPNHYK TGFWGRAQV VHYAMSFLV SWLHCSEEA RQALFQSPN FSIWLQRLV LEDPEPAVR REVCSAIYR LCLGISTSG DMLENSLVA PMLSELLLY LDNAESMRS QKFEAIPVL EEGKEPYGP ACRDYFWLV ARLVDSLPD DLVKESLED PQNCVIDIN GLTNKISRS LLSREYLET RHRTIDDDG LIGLLNLLT NLIKHRPPF QVSKQGQEL LVEVFKFLF ALPSPKLRH VPKCKSPRS RTAAYDLLV ELVKGAPQN YYILHEKLL KQHQPGPHS PYPWDYWPH EDGRSECGY VGLTNLGAT CYMASCMQH LYMMPQARA SILAANTEH SKHEPTLKE LQRMFAYLL ESERKAYNP RSFCKVYTM DHQPLNTAE QKDMAEFFI DLVSKLEEM TPALKTVVK KLFCGVLSN NVVSLDCGH VSRTLEEFY TVRCQVADM RNLYESLDE VTVKDTLEG DNMYTCSQC GKKVRAEKR ACFKKLPHI LCFNTMRYT FNMLTMLKE KVNTHFSFP LRLNMAGYV EKQLMPQHY QEDKLKQYE PEDEQYEYD LIGVTVHTG TADGGHYYS FIKDRTAGS RDKWFLFND AEVKPFDPN QIAAECFGG EMTSKTYDS VTDKFMDFS FEKTNSAYM LFYERCPIV TSESSESSS TTPESGESS QILSNTSAA TFELSKELE DWIWQDNMH FIQDKNIFE HTYFNFMWQ ICGYIPQTI LPIEPDITQ KAAQLSTSF FIETFIHAK EKPTMVQWV ELVTKQFNA CQEACVWFL EHMANDTWW PVQVLLKCP NQMVRQMFQ RLCIHVIQR LKQSHSSLY LEIDRDIPS ENKTVEPSK IGNASCVTK FIKTLLSLM EHGAKAHLK HLTEYFGFL YEFSKMGEE ESKFLLAVG AINSMVHFY LGQKANDFV DVSEGEEEE EVVALPSDK YKPASLDKM ITLVASLVE KSRDSNMKL QLSEKDYSA VAEGKGFPF LYQQIKDNI NLQQTRNLI HSLCRGNNQ LAQSIICMI SQAISRHSD TSQPFFKIL TLLTESTAQ GSGGSSSQP CFTQLVLQK VWEAAECCP YSALDWLAL QVVRNRLVH QWVLSSMDS WLEHFLIAH SNQRVRNAA GYLLVSLVP SAPFRQGFR AAHRMQREP QLSNEAQTV LHQIYTGLL RLLGSAKHY TDMQQHGTM KLTTYFALL MYCCVSRTE KLMFGQYFL QLWHLFHPK LSEPSIPAY HNKQALLAF WNHVCVDCP ENVQLMLQN AHVTKNIAF NYILADHDD QEIVLYNRA MLPAYYGLL RMMCQQSRI FTRHLAQHQ NLQWAFKNI TPHPTQYPQ AVEELFKLM QLMVMKHPD MTEAEQREI CSFRRNTLM TYLQCLDGR TSWGTLISA FRILLESND DKLYVAYNG GLQIVFECF QNLHIMLHE ATACHVSGD LMDILTIMQ DLVRCVRTY RDNKDARGI LLATKDWLE VLRKLATLL NTYNPPEMR SLCIDVLKE FVLIMAPEV MQILVPLLS HCHSAFQDS HDAVPLGPY FPRRGHSLP VVAGKGGAR PLRPMVQMT VPHNQLECS RGVDSEYDE ALDKFYTPY HDFIDMLFR LAVNNDTVT EVLVNLSAV VGFEAVPLH STYFPKLWL DILKAQHID RKYISMLTS CNYFVDYID AVLLDERSA LTVNVIYEF LVAFFPKVS AHVLSEQTL TMIDNTVVA LSEQVGSLE VTKMAKRLA GDLRALILV YNSPEAVPP PLLRNTLKQ LQSKVVVEI EKSSKDKET GESSERKED QPSTSKDTS DKKSDSDSE TQNTSCSDD SADEKKGPV ENLKLLDTI LNELLAIVD KKA
- >TC009817 RA
atgtgtgat gcatgttcg gattttgta caactcctt gtttcgtat gaagcgcaa gtgtgcaga gagagcaat gaacagccg accctaacc aagcacgaa atcgacgtt atttacgtt tacgtgcag acatggccg gccagacaa tgcatgtgc tgttatcgc gactcaaaa aacctggaa aggttcagt tacatcacc cagggcctg atctacatg gtcgtatgc cagctgaaa caattggcc gaaaaaggt tcagacgca aaaaaagag gaaaataag gaagccgcc gataaagag aacgaggtc caagaccgc gataaatta ctgatttta gtgtctaaa gtgtttcta ttaaatttt ccgctttat gttgcctac aaacacacg attcaggcc aaactcgac gaactgacg caacaagaa atgcaaaat ctgaacctt ttctgcgac ctacacgac tcggaaata ccgatttat ttattgaaa aatgttagc tggttttgt aaaatgggg gggctgttg gccatgacg gcgtgtttc acgcagttg gcgccggaa caactgccg gtttccacc gcacatgcg atgatttcg attgtcagc aatttaaaa ttgtggatg aactacaag agtatggtt tcaatgttg gtacctttg cgttcttgt gtcttacgt tacatgtgt aaattatca gatcaggat ttgcgggcg cctaccaca aaaagcatg gcagacttt atgtggaat gcaattaag gacccacta gatactcct gttgcattc gacattgac ggcctagat ttagcgttt aagtatttc acttctagt actttaaca atgcgactt gcggggatt acccaaatt aataaccag ataggcgtt ctggctgaa atttgcgtc ggggaatcc ctgccggag gcggaagcc gccccgaga cacatggcc gactggttg atcaacaac aatatcatt tcgcatatt tttggaccg aatttacac gtcgaggtc attaaacaa agtcacatt attttaaat tttctcgca atggaaggg agaataact tgcgaacac atggatata atttgggcc gcggcccaa ttaaaacat tgctcaaag ccggtccat gacttactt cccggactt gttaaacat ttagcggcc tcaccgact ttgcatttg tacagtctt ttgaccaaa ctagaaccg aaggagcac acagaacaa actttgtat ctagcgtcg gctctgatt aaggctgtg tggtcacgt ggtggcggt gtaccggaa gtgggtttg ataaatgta gcggcatct aatgccggg attacgtta cataaatgt gcaacatcg tcggaaaat agtgtcagt attgatccg agcaattcc gaggaagaa catggtgag agtacggga cagtctgaa agccacaaa tcggacagc gaggatatt gatggcgta gaaatagat gaagacgat ggccccatt gatgggggc aatcctggg cccccaccg tgtaaatta gcccggaaa caagcgata gctcgggtt cactgtgat ggaagtagt accgacccg gaaatcgat ggtattcca attaaatca ccggctgaa aaacccaag cgatgtgcg gttgttccg aaagtcaaa gccgagaaa cgacaaatg tcgtccgtc aggaaaaaa atattatca actttaaac gtcctgcct gaagggatt gaagccata ggttcgagc agctctcaa gacgaggcc gatgtcgaa gaagttccg gccaagttc cgcaaacgt cgcaaaatg ctccgtaaa acgcgggtc aagcgccaa aaaggtgtt cataaacgt gtagtggcg attagcgga attgaagct ttgagcgaa gtcgaggta gaaagcgat tcggaaatg aaggcggct ttgaaaggc gtcgcggcg gcggcgttg gtgctcccg gcgcaaatg agcggtttg gtcgaaacg aatttaaaa ccggccgat tacttggat gagagctcg tcttgtagc gaattgggt aaagattcg gtcacgttg gaaagccac gaggccgcc tgtaccaat tttcttact ctgcactct cgcggaggt catattttg gaaatgctg agcggggag gaggccgaa gttgaagga gacgccgac gggagttac tcctcgagg atgagcaac aagtccgag aaaaatatg gctgatttc gacggcgag gactcggtg tgtgaggag gagttggct cagttaaac gaacatgcc cagttgaac cttcacgct ttagccaac caatcgccg gaaaagtcc ccagttaga gttgatccc agactttcg gcgtgtttt aaggccgac aatgtttgc cagcctggg aatacactt ttatgggat ttgctccag gatgataaa atttgtcac ttaggcgaa ggtctcgca ttagaagcc gaaaaaact ctatgtaat ttaatctgt tatacaccg gataaagaa attcggatg aaatttatc gaaggttgt ttacataat ttagccaac aatgtttct gtggttgtg tcgttacga atgttacct aagttattg acctcgttc cgagggatt gatatgcac cacgtgacc cattgggcc gaacgtcag caccacatg atgaaacat tttttcaat aatttaaaa acttacaca gccaatcct tcaaaatcc ttacctctc tacacacac caaatgcaa gttcaggtc cggttacat tttctttcg gctgttttt tcaccagtg gtgtcccca aacaccttc aagctgagt atcgaacaa gtcgataca ttatgggaa tgtctggct cacgatccc gaatgttcg gatgaactt ttcagttgg ttactctca caaactaaa accactgaa ctccacgct ttaaaaatt gacgctttg agacgcctg tacatgcat catttgccg agtctaccg ccggagaaa ttcagtatg atttgtttg agtcttttc cagcagttg tgtagcttg aatcattta ttaacgata aatatgggc tccgaatcg gtccgagag agttacaac gtcggaatg gatcatatg tggaaaata gcgttgagg gcgaataac actgatgtc agtatggca gctattcag tacataaat agttactac atgggtcag aacttgcaa aacgagggc cagtttgta tggcaatgt atgacccat ttgaaggct gcgaccgag gacttggtc agtgtgagc actccgggg gccgaggaa gcgcctctt ttgtgcatc cagagggca ctgttgctc atgaaaagc caccttgag actttccgg cgacgctat gcttatcac ttgaggagg tggacgctg gagggcaaa ggcataggg agtcactca gcctcgtcg gttgaccgc tgtggcagc cctttgagg cttatcgtc cagtcggga agttcgacc gagcggtgc taccttgat atgttaagt tcggactat gtcgccgac cttcgggcc gaaatagta aaatggtcc gaaggtcca agtcataca aaaaatcaa gagtccgaa tccagtcac aaaaccaac gatacgtgg attcgactc ataacacaa ggccaggag ttaactata gactgcgac gagaagact ttggccgaa atgggtttc aaagacaac caaatggtg tacatttcg atcggcgtg tccaaaaac atgaaaaag agggagtgt ttagatttg ccatcgatg caaccgcct ccacctcgc gaatcaatt cccactcta ctcctctta agaccgaat tatttcgag cagttgttt tcgcttatg catacattg agttcaatg aaagtacca gtgaaaggc gggcaccat atccccaat acaaaagct caagttttg agtcggcga gtttgggat attttaagc ttgttgccg acgaaccca accctacta cagggcttc aaacagtta gatacgccc ctaactgag ttacttgac ccgtcgagt gcacaaaaa ttgatgtat tcgctgtac atcgtagag tcactcagt gcgaaaatt tccaaacct ggagaaaac gataaggag tcatggagc cgcactttt atccaacac ggcgggtta cgccattta tttgatatt tttatgtca ggggtttta caacgcgac gggggcaac ggcagcgaa tggcaacaa gactgtctt gcttcctta cttaaaata atttgtcaa ttgggggtg gagtccctt ccaatcgaa tcccgcccg gttcggagc gataaaatc gtgatacca aacctgaac gaagcgatg ctcgcaatg atcgatgtt aagaacaca atgccccaa ctcacaagc atcctcgaa gaggcgtca ctaccgaaa gaccccaat cattacaag accggtttc tgggggcgc gcccaagtc gtccattac gccatgtct ttcctcgtc tcgtggtta cattgcagt gaagaggcc cgacaggcc ctttttcaa tcgccaaat ttctcgatt tggttgcaa agactcgtc ttagaggac cccgagcct gcagtacgg cgcgaagtt tgctcggcg atttacaga ttatgtttg ggaatttcg accagcggt gacatgctc gagaatagt ttggtcgcc cccatgctc tctgaatta ctcctctac ttggataat gcggaaagt atgcggtcg caaaaattc gaagcgatc ccggtcctg gaagagggc aaggagccc tacgggcca gcttgtagg gattatttc tggctcgtg gcgcggctt gttgatagt ttacctgac gatttagtt aaagagagt ctcgaagat ccgcagaat tgtgttatt gacatcaac ggactgacg aataaaatt tcgaggtcg ctgttatcg agagagtat ctcgagaca agacatcga acaatagat gatgatggc ttgatcggg ttgttgaat ttgttgacg aatttgatt aaacacaga ccgccgttt caagtcagt aagcaaggg caggagttg ttggttgaa gtttttaaa ttcttgttt gctttacca agtcctaag ttgaggcac gtgcctaaa tgtaagtcg ccgaggtcg aggacagct gcttatgat cttttagtg gagctggtc aagggggcg ccgcagaat tactatatt ttgcacgag aagttgctc aaacaacac caaccaggg ccccattcg ccctaccct tgggactac tggccccac gaagacggc cgttccgaa tgcggttac gtcggttta acgaattta ggagccacc tgttacatg gcatcatgt atgcaacac ctctacatg atgccccaa gctagagcc tcgattttg gccgcaaat accgaacat tcgaaacac gaaccgact ctcaaggag ttgcaacgg atgtttgcg tacttactg gagagtgaa aggaaggct tacaacccc cgcagcttc tgcaaggtc tacaccatg gatcaccag ccgctcaat accgccgag caaaaagac atggccgaa ttcttcatc gacttggtc agcaaattg gaagaaatg actccggcg ttaaaaacc gtggtgaag aaattattc tgcggtgta cttagcaac aacgttgtg tcattagac tgtggccat gttagtcgg acgttagaa gaattttat acggttcgg tgccaagtc gctgatatg cgaaatctt tacgaatca cttgatgaa gtcacagta aaagatact ctcgaaggc gataatatg tacacgtgt tcgcaatgt ggtaaaaaa gtgcgcgcc gaaaaaagg gcctgtttt aaaaaatta ccgcatatt ttgtgcttc aatacgatg agatacact ttcaatatg ttgacgatg ctcaaggaa aaagtgaat acgcatttt tcgtttccg ttgaggcta aatatggcc ggatatgtc gaaaaacaa ttaatgccg cagcattat caggaggat aaattaaaa caatatgag ccggaagat gaacagtat gagtacgac ctaatcgga gtcacggtt cacactggg acggctgac ggtggccat tattacagt tttatcaaa gatcgaact gcaggttcg cgcgataaa tggtttttg tttaacgat gctgaagtc aaaccgttt gatccgaat cagatagcg gctgagtgt ttcggtggc gaaatgacg agtaaaaca tacgacagc gtcacggac aaattcatg gattttagt ttcgaaaaa acgaatagt gcttatatg cttttctac gagcgttgt ccgattgtg acttcggaa agttccgaa agttcttca acaacgccc gaatctggt gagagcagt caaatactt agtaacaca tctgcggcc actttcgag ttgagtaaa gagttggag gattggatt tggcaggat aacatgcat ttcattcag gataaaaat attttcgaa catacttac tttaatttc atgtggcaa atttgcggg tacataccg cagactatc ctaccgatc gagccggat atcacgcag aaggcggcc caattgtcc acttcgttt ttcattgag acttttatt catgccaaa gagaagccg acgatggta caatgggtt gagcttgta acgaagcag tttaatgca tgtcaggag gcttgcgtg tggttcctg gaacacatg gcaaacgac acttggtgg ccagttcaa gtattatta aaatgtccg aatcaaatg gtgcgacaa atgtttcaa aggctgtgc atacatgtg atacaacgc ttgaagcag tcacattct agtttatat ttagaaatc gatcgagac attccatcg gaaaataaa acggtcgaa ccttcgaaa atcggaaat gcgtcgtgt gtgacgaaa ttcatcaaa actttatta tcacttatg gagcacggg gcgaaagcc catttaaaa cacttgacg gaatatttc ggttttttg tacgaattt agtaaaatg ggtgaagaa gaatcaaag tttttgcta gctgtaggt gctattaac tcgatggtg catttttat ttgggacaa aaagcgaac gattttgtt gatgtaagc gaaggtgag gaagaagag gaagtcgtt gctttacct agtgataag tataaaccg gcctctctt gataaaatg ataacacta gtagcgtca ctagtcgaa aaaagtcgt gactcgaat atgaaatta caattaagt gagaaagat tatagcgca gtggccgaa ggcaaaggt tttccgttt ctgtatcaa caaatcaaa gacaacata aatttgcag caaacgcgt aatttaatc cattcgctg tgccgaggg aacaaccag ttagctcag tcgataatt tgcatgatc tcacaggcg atttcgaga cattccgat acctcccaa ccctttttc aaaatccta accctcctt accgaaagt acagcccaa ggttcaggt ggttcatca tcccagcca tgtttcaca cagttagtt ttacagaag gtgtgggaa gcggcagaa tgttgtccc tattcggcg ttggattgg ttggctctg caagtggtg agaaatcgg ttggtgcat cagtgggtc ttaagttcg atggattcg tggctggaa cacttcctc atagcccac agcaaccaa cgcgttcga aatgccgcc ggttatttg ctcgtcagt ctggtgcca agcgccccc ttccggcaa ggcttccgc gccgcgcat cgcatgcaa agagagccc caactctcc aacgaggcc caaacggtg cttcaccaa atttacaca ggcctgttg aggctacta ggctcggct aagcactat acggacatg caacagcac ggcacgatg aagctaaca acgtacttt gcactcttg atgtattgt tgtgtgtca aggacggag aagttgatg ttcggtcag tatttcctg caattgtgg cacctgttt catccgaaa ttatcggag ccttcaata cctgcatat cacaataaa caggcgctg ttggcgttt tggaaccac gtttgtgtc gactgcccc gaaaatgtg caattaatg ttacagaat gctcacgtt acgaaaaat atcgctttt aattatatt cttgccgac cacgatgac caagagatt gtcttgtac aaccgtgcc atgttgcca gcgtactac gggttgttg cgaatgatg tgtcaacag tctcgtatt ttcacgaga catttagcc caacaccag aatttgcaa tgggctttt aaaaatatc acgccacat ccgactcaa tacccgcaa gcggtcgaa gaattgttc aagttaatg caactaatg gtcatgaag catcccgac atgactgaa gccgaacaa agagaaatc tgtagtttc agacgaaac acattgatg acgtatttg caatgtttg gacggtagg acaagttgg gggactttg atttcggcg tttaggatt ttattagag tcgaacgat gataaactt tacgtggcc tacaatgga ggcttgcag attgttttt gaatgtttc caaaacttg cacataatg ttgcacgag gccaccgct tgccacgtc tctggagac ctgatggac atcctcacc ataatgcaa gacctcgtc cgctgcgtg cgcacctac cgtgacaat aaagacgcc cgtggtatt ctcctcgcc acaaaagac tggctggaa gtcctccgc aagttagca acactgctc aacacgtac aacccgccg gaaatgcgc tcgttatgc atcgacgtc ttgaaggag ttcgtcctg atcatggca ccggaagtg atgcagatt ttggtgcct ctgttgtcg cattgccat tctgccttc caggacagc cacgatgcg gtgcccctg ggcccctat ttccctcga cgggggcat tctctgccc gttgtggcc gggaaaggg ggcgcacgg cccctaaga ccgatggtg cagatgacg gtgccccat aatcagtta gagtgcagt cgcggggtt gattctgaa tacgacgaa gcgctggat aagttttac acgccgtac catgatttc atcgacatg ttgttccga ctggcggtg aataacgac acggtgacg gaggttctg gttaatttg agtgcggtg gttgggttc gaggcggtg ccgttgcat tctacgtat tttccgaag ttgtggttg gatatattg aaggcgcaa cacattgac cgcaagtac atctcgatg ctgacgagt tgtaattat ttcgtcgac tacattgac gctgttctg ctcgacgaa agatcagcc cttacagta aacgtaatt tacgaattc ttggtggca tttttcccg aaagtctcg gcgcacgtc ctttcggaa caaaccctc acaatgatc gacaacacg gtggtggct ctgagcgaa caagtgggg tcgttggag gtcacaaaa atggcgaaa cggctggcg ggtgatttg cgagcgctg attttggtg tacaatagt cccgaggct gtgccgccg cctttgctt agaaacacg ctcaaacaa ctgcagagt aaagtcgtg gtggagatc gagaagagt agtaaagat aaggaaacg ggtgagagt agcgaaaga aaggaagat caaccgagc acgtccaaa gacactagt gataaaaaa agtgacagt gattcggag acgcaaaat acatcgtgt agtgacgat agtgcggat gagaagaaa ggtccagtg gaaaatttg aagttgttg gatacgata ctgaacgaa ttactcgcc attgttgat aaaaaggct taa
- >TC009817 RA
tttgactaa aaacgtttc aaaatggct gatgtttgt cgttaaatc tacaccatc tgatctaaa ataccgaat aaaacgtac cgaaagaag ttttgccgt tttgcattc gcaaaattg cgatgaaaa tcccttaaa ttttgcttt acaggctta gacatgtgt gatgcatgt tcggatttt gtacaactc cttgtttcg tatgaagcg caagtgtgc agagagagc aatgaacag ccgacccta accaagcac gaaatcgac gttatttac gtttacgtg cagacatgg ccggccaga caatgcatg tgctgttat cgcgactca aaaaacctg gaaaggttc agttacatc acccagggc ctgatctac atggtcgta tgccagctg aaacaattg gccgaaaaa ggttcagac gcaaaaaaa gaggaaaat aaggaagcc gccgataaa gagaacgag gtccaagac cgcgataaa ttactgatt ttagtgtct aaagtgttt ctattaaat tttccgctt tatgttgcc tacaaacac acgattcag gccaaactc gacgaactg acgcaacaa gaaatgcaa aatctgaac cttttctgc gacctacac gactcggaa ataccgatt tatttattg aaaaatgtt agctggttt tgtaaaatg ggggggctg ttggccatg acggcgtgt ttcacgcag ttggcgccg gaacaactg ccggtttcc accgcacat gcgatgatt tcgattgtc agcaattta aaattgtgg atgaactac aagagtatg gtttcaatg ttggtacct ttgcgttct tgtgtctta cgttacatg tgtaaatta tcagatcag gatttgcgg gcgcctacc acaaaaagc atggcagac tttatgtgg aatgcaatt aaggaccca ctagatact cctgttgca ttcgacatt gacggccta gatttagcg tttaagtat ttcacttct agtacttta acaatgcga cttgcgggg attacccaa attaataac cagataggc gttctggct gaaatttgc gtcggggaa tccctgccg gaggcggaa gccgccccg agacacatg gccgactgg ttgatcaac aacaatatc atttcgcat atttttgga ccgaattta cacgtcgag gtcattaaa caaagtcac attatttta aattttctc gcaatggaa gggagaata acttgcgaa cacatggat ataatttgg gccgcggcc caattaaaa cattgctca aagccggtc catgactta cttcccgga cttgttaaa catttagcg gcctcaccg actttgcat ttgtacagt cttttgacc aaactagaa ccgaaggag cacacagaa caaactttg tatctagcg tcggctctg attaaggct gtgtggtca cgtggtggc ggtgtaccg gaagtgggt ttgataaat gtagcggca tctaatgcc gggattacg ttacataaa tgtgcaaca tcgtcggaa aatagtgtc agtattgat ccgagcaat tccgaggaa gaacatggt gagagtacg ggacagtct gaaagccac aaatcggac agcgaggat attgatggc gtagaaata gatgaagac gatggcccc attgatggg ggcaatcct gggccccca ccgtgtaaa ttagcccgg aaacaagcg atagctcgg gttcactgt gatggaagt agtaccgac ccggaaatc gatggtatt ccaattaaa tcaccggct gaaaaaccc aagcgatgt gcggttgtt ccgaaagtc aaagccgag aaacgacaa atgtcgtcc gtcaggaaa aaaatatta tcaacttta aacgtcctg cctgaaggg attgaagcc ataggttcg agcagctct caagacgag gccgatgtc gaagaagtt ccggccaag ttccgcaaa cgtcgcaaa atgctccgt aaaacgcgg gtcaagcgc caaaaaggt gttcataaa cgtgtagtg gcgattagc ggaattgaa gctttgagc gaagtcgag gtagaaagc gattcggaa atgaaggcg gctttgaaa ggcgtcgcg gcggcggcg ttggtgctc ccggcgcaa atgagcggt ttggtcgaa acgaattta aaaccggcc gattacttg gatgagagc tcgtcttgt agcgaattg ggtaaagat tcggtcacg ttggaaagc cacgaggcc gcctgtacc aattttctt actctgcac tctcgcgga ggtcatatt ttggaaatg ctgagcggg gaggaggcc gaagttgaa ggagacgcc gacgggagt tactcctcg aggatgagc aacaagtcc gagaaaaat atggctgat ttcgacggc gaggactcg gtgtgtgag gaggagttg gctcagtta aacgaacat gcccagttg aaccttcac gctttagcc aaccaatcg ccggaaaag tccccagtt agagttgat cccagactt tcggcgtgt tttaaggcc gacaatgtt tgccagcct gggaataca cttttatgg gatttgctc caggatgat aaaatttgt cacttaggc gaaggtctc gcattagaa gccgaaaaa actctatgt aatttaatc tgttataca ccggataaa gaaattcgg atgaaattt atcgaaggt tgtttacat aatttagcc aacaatgtt tctgtggtt gtgtcgtta cgaatgtta cctaagtta ttgacctcg ttccgaggg attgatatg caccacgtg acccattgg gccgaacgt cagcaccac atgatgaaa cattttttc aataattta aaaacttac acagccaat ccttcaaaa tccttacct ctctacaca caccaaatg caagttcag gtccggtta cattttctt tcggctgtt ttttcacca gtggtgtcc ccaaacacc ttcaagctg agtatcgaa caagtcgat acattatgg gaatgtctg gctcacgat cccgaatgt tcggatgaa cttttcagt tggttactc tcacaaact aaaaccact gaactccac gctttaaaa attgacgct ttgagacgc ctgtacatg catcatttg ccgagtcta ccgccggag aaattcagt atgatttgt ttgagtctt ttccagcag ttgtgtagc ttgaatcat ttattaacg ataaatatg ggctccgaa tcggtccga gagagttac aacgtcgga atggatcat atgtggaaa atagcgttg agggcgaat aacactgat gtcagtatg gcagctatt cagtacata aatagttac tacatgggt cagaacttg caaaacgag ggccagttt gtatggcaa tgtatgacc catttgaag gctgcgacc gaggacttg gtcagtgtg agcactccg ggggccgag gaagcgcct cttttgtgc atccagagg gcactgttg ctcatgaaa agccacctt gagactttc cggcgacgc tatgcttat cacttgagg aggtggacg ctggagggc aaaggcata gggagtcac tcagcctcg tcggttgac cgctgtggc agccctttg aggcttatc gtccagtcg ggaagttcg accgagcgg tgctacctt gatatgtta agttcggac tatgtcgcc gaccttcgg gccgaaata gtaaaatgg tccgaaggt ccaagtcat acaaaaaat caagagtcc gaatccagt cacaaaacc aacgatacg tggattcga ctcataaca caaggccag gagttaact atagactgc gacgagaag actttggcc gaaatgggt ttcaaagac aaccaaatg gtgtacatt tcgatcggc gtgtccaaa aacatgaaa aagagggag tgtttagat ttgccatcg atgcaaccg cctccacct cgcgaatca attcccact ctactcctc ttaagaccg aattatttc gagcagttg ttttcgctt atgcataca ttgagttca atgaaagta ccagtgaaa ggcgggcac catatcccc aatacaaaa gctcaagtt ttgagtcgg cgagtttgg gatatttta agcttgttg ccgacgaac ccaacccta ctacagggc ttcaaacag ttagatacg cccctaact gagttactt gacccgtcg agtgcacaa aaattgatg tattcgctg tacatcgta gagtcactc agtgcgaaa atttccaaa cctggagaa aacgataag gagtcatgg agccgcact tttatccaa cacggcggg ttacgccat ttatttgat atttttatg tcaggggtt ttacaacgc gacgggggc aacggcagc gaatggcaa caagactgt cttgcttcc ttacttaaa ataatttgt caattgggg gtggagtcc cttccaatc gaatcccgc ccggttcgg agcgataaa atcgtgata ccaaacctg aacgaagcg atgctcgca atgatcgat gttaagaac acaatgccc caactcaca agcatcctc gaagaggcg tcactaccg aaagacccc aatcattac aagaccggt ttctggggg cgcgcccaa gtcgtccat tacgccatg tctttcctc gtctcgtgg ttacattgc agtgaagag gcccgacag gcccttttt caatcgcca aatttctcg atttggttg caaagactc gtcttagag gaccccgag cctgcagta cggcgcgaa gtttgctcg gcgatttac agattatgt ttgggaatt tcgaccagc ggtgacatg ctcgagaat agtttggtc gcccccatg ctctctgaa ttactcctc tacttggat aatgcggaa agtatgcgg tcgcaaaaa ttcgaagcg atcccggtc ctggaagag ggcaaggag ccctacggg ccagcttgt agggattat ttctggctc gtggcgcgg cttgttgat agtttacct gacgattta gttaaagag agtctcgaa gatccgcag aattgtgtt attgacatc aacggactg acgaataaa atttcgagg tcgctgtta tcgagagag tatctcgag acaagacat cgaacaata gatgatgat ggcttgatc gggttgttg aatttgttg acgaatttg attaaacac agaccgccg tttcaagtc agtaagcaa gggcaggag ttgttggtt gaagttttt aaattcttg tttgcttta ccaagtcct aagttgagg cacgtgcct aaatgtaag tcgccgagg tcgaggaca gctgcttat gatctttta gtggagctg gtcaagggg gcgccgcag aattactat attttgcac gagaagttg ctcaaacaa caccaacca gggccccat tcgccctac ccttgggac tactggccc cacgaagac ggccgttcc gaatgcggt tacgtcggt ttaacgaat ttaggagcc acctgttac atggcatca tgtatgcaa cacctctac atgatgccc caagctaga gcctcgatt ttggccgca aataccgaa cattcgaaa cacgaaccg actctcaag gagttgcaa cggatgttt gcgtactta ctggagagt gaaaggaag gcttacaac ccccgcagc ttctgcaag gtctacacc atggatcac cagccgctc aataccgcc gagcaaaaa gacatggcc gaattcttc atcgacttg gtcagcaaa ttggaagaa atgactccg gcgttaaaa accgtggtg aagaaatta ttctgcggt gtacttagc aacaacgtt gtgtcatta gactgtggc catgttagt cggacgtta gaagaattt tatacggtt cggtgccaa gtcgctgat atgcgaaat ctttacgaa tcacttgat gaagtcaca gtaaaagat actctcgaa ggcgataat atgtacacg tgttcgcaa tgtggtaaa aaagtgcgc gccgaaaaa agggcctgt tttaaaaaa ttaccgcat attttgtgc ttcaatacg atgagatac actttcaat atgttgacg atgctcaag gaaaaagtg aatacgcat ttttcgttt ccgttgagg ctaaatatg gccggatat gtcgaaaaa caattaatg ccgcagcat tatcaggag gataaatta aaacaatat gagccggaa gatgaacag tatgagtac gacctaatc ggagtcacg gttcacact gggacggct gacggtggc cattattac agttttatc aaagatcga actgcaggt tcgcgcgat aaatggttt ttgtttaac gatgctgaa gtcaaaccg tttgatccg aatcagata gcggctgag tgtttcggt ggcgaaatg acgagtaaa acatacgac agcgtcacg gacaaattc atggatttt agtttcgaa aaaacgaat agtgcttat atgcttttc tacgagcgt tgtccgatt gtgacttcg gaaagttcc gaaagttct tcaacaacg cccgaatct ggtgagagc agtcaaata cttagtaac acatctgcg gccactttc gagttgagt aaagagttg gaggattgg atttggcag gataacatg catttcatt caggataaa aatattttc gaacatact tactttaat ttcatgtgg caaatttgc gggtacata ccgcagact atcctaccg atcgagccg gatatcacg cagaaggcg gcccaattg tccacttcg tttttcatt gagactttt attcatgcc aaagagaag ccgacgatg gtacaatgg gttgagctt gtaacgaag cagtttaat gcatgtcag gaggcttgc gtgtggttc ctggaacac atggcaaac gacacttgg tggccagtt caagtatta ttaaaatgt ccgaatcaa atggtgcga caaatgttt caaaggctg tgcatacat gtgatacaa cgcttgaag cagtcacat tctagttta tatttagaa atcgatcga gacattcca tcggaaaat aaaacggtc gaaccttcg aaaatcgga aatgcgtcg tgtgtgacg aaattcatc aaaacttta ttatcactt atggagcac ggggcgaaa gcccattta aaacacttg acggaatat ttcggtttt ttgtacgaa tttagtaaa atgggtgaa gaagaatca aagtttttg ctagctgta ggtgctatt aactcgatg gtgcatttt tatttggga caaaaagcg aacgatttt gttgatgta agcgaaggt gaggaagaa gaggaagtc gttgcttta cctagtgat aagtataaa ccggcctct cttgataaa atgataaca ctagtagcg tcactagtc gaaaaaagt cgtgactcg aatatgaaa ttacaatta agtgagaaa gattatagc gcagtggcc gaaggcaaa ggttttccg tttctgtat caacaaatc aaagacaac ataaatttg cagcaaacg cgtaattta atccattcg ctgtgccga gggaacaac cagttagct cagtcgata atttgcatg atctcacag gcgatttcg agacattcc gatacctcc caacccttt ttcaaaatc ctaaccctc cttaccgaa agtacagcc caaggttca ggtggttca tcatcccag ccatgtttc acacagtta gttttacag aaggtgtgg gaagcggca gaatgttgt ccctattcg gcgttggat tggttggct ctgcaagtg gtgagaaat cggttggtg catcagtgg gtcttaagt tcgatggat tcgtggctg gaacacttc ctcatagcc cacagcaac caacgcgtt cgaaatgcc gccggttat ttgctcgtc agtctggtg ccaagcgcc cccttccgg caaggcttc cgcgccgcg catcgcatg caaagagag ccccaactc tccaacgag gcccaaacg gtgcttcac caaatttac acaggcctg ttgaggcta ctaggctcg gctaagcac tatacggac atgcaacag cacggcacg atgaagcta acaacgtac tttgcactc ttgatgtat tgttgtgtg tcaaggacg gagaagttg atgttcggt cagtatttc ctgcaattg tggcacctg tttcatccg aaattatcg gagccttca atacctgca tatcacaat aaacaggcg ctgttggcg ttttggaac cacgtttgt gtcgactgc cccgaaaat gtgcaatta atgttacag aatgctcac gttacgaaa aatatcgct tttaattat attcttgcc gaccacgat gaccaagag attgtcttg tacaaccgt gccatgttg ccagcgtac tacgggttg ttgcgaatg atgtgtcaa cagtctcgt attttcacg agacattta gcccaacac cagaatttg caatgggct tttaaaaat atcacgcca catccgact caatacccg caagcggtc gaagaattg ttcaagtta atgcaacta atggtcatg aagcatccc gacatgact gaagccgaa caaagagaa atctgtagt ttcagacga aacacattg atgacgtat ttgcaatgt ttggacggt aggacaagt tgggggact ttgatttcg gcgtttagg attttatta gagtcgaac gatgataaa ctttacgtg gcctacaat ggaggcttg cagattgtt tttgaatgt ttccaaaac ttgcacata atgttgcac gaggccacc gcttgccac gtctctgga gacctgatg gacatcctc accataatg caagacctc gtccgctgc gtgcgcacc taccgtgac aataaagac gcccgtggt attctcctc gccacaaaa gactggctg gaagtcctc cgcaagtta gcaacactg ctcaacacg tacaacccg ccggaaatg cgctcgtta tgcatcgac gtcttgaag gagttcgtc ctgatcatg gcaccggaa gtgatgcag attttggtg cctctgttg tcgcattgc cattctgcc ttccaggac agccacgat gcggtgccc ctgggcccc tatttccct cgacggggg cattctctg cccgttgtg gccgggaaa gggggcgca cggccccta agaccgatg gtgcagatg acggtgccc cataatcag ttagagtgc agtcgcggg gttgattct gaatacgac gaagcgctg gataagttt tacacgccg taccatgat ttcatcgac atgttgttc cgactggcg gtgaataac gacacggtg acggaggtt ctggttaat ttgagtgcg gtggttggg ttcgaggcg gtgccgttg cattctacg tattttccg aagttgtgg ttggatata ttgaaggcg caacacatt gaccgcaag tacatctcg atgctgacg agttgtaat tatttcgtc gactacatt gacgctgtt ctgctcgac gaaagatca gcccttaca gtaaacgta atttacgaa ttcttggtg gcatttttc ccgaaagtc tcggcgcac gtcctttcg gaacaaacc ctcacaatg atcgacaac acggtggtg gctctgagc gaacaagtg gggtcgttg gaggtcaca aaaatggcg aaacggctg gcgggtgat ttgcgagcg ctgattttg gtgtacaat agtcccgag gctgtgccg ccgcctttg cttagaaac acgctcaaa caactgcag agtaaagtc gtggtggag atcgagaag agtagtaaa gataaggaa acgggtgag agtagcgaa agaaaggaa gatcaaccg agcacgtcc aaagacact agtgataaa aaaagtgac agtgattcg gagacgcaa aatacatcg tgtagtgac gatagtgcg gatgagaag aaaggtcca gtggaaaat ttgaagttg ttggatacg atactgaac gaattactc gccattgtt gataaaaag gcttaattt ctgattttg tattgacca atctaagtg ttctttaca cgcttttct atgtgtttc agattttgt agatttttg tatattttt agctttaat atcggccga gtgtcggaa attagcagg gttttgcct taagtatga atttttatt actttaaga atggatcta atataacgt cgttgaagt gcttgtaac tctcattgt ttcagctta cattttcac tgtaaactt tttcattac aagactgtt acaataaaa tatctcttt tatatgttg cagtgtgac tttttctac cacctgttg cgaaataaa aataatata acgtg
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