Status
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Gene information
General information
TC009797 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8234620..8237296
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009797
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10340

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009797 RA
      MSVGLSESQ NYIWSDDDC LGEGTFGKV FRGIQKRSG 
      DKVAIKIFK HVNHQVSQE TKLLQSLQH KHIIRFFAT 
      EFELSTNKM VMILELCNG GSLMSYLNE PENVCGLPD 
      EEFLLVFNH LSAGLEFLK RHDVIHRDV KPDNIMRHV 
      LDDGQTVYK LADFGTARE LPEGQNFTS LHGTEPYLH 
      PQLYRVCFF QGSNCTREF GANTDLWSI GVTLYQTAT 
      GNLPFLVMD RHVMLRIYH EMQENEGIV SAIQQKNSD 
      EVVFQKKLP RNCRLSCGL KQLITPIIA GVFIQEKGK 
      QWSFTQYYE QVVDLVSRK VINVFYVNK MRLMKVYIK 
      SDETFNTFK YYVSAQSEV AEENQVFLY KANLIVNTF 
      EDFSGVDES DCIFLFDRS NAEVESTID VQLVFENLT 
      NFPNKFMSS SESDDHRLA FVTCRDLCL CKNVMDYQS 
      IVYKYFSNF IKDFNSYCK EETRDQRKM YDTLLEKSS 
      QTKNRAQIL KQLQNLNHS NDDSFLDRF EEISDNFLS 
      ETDKLFNDL RNIKADLVP NTSHDSYLK TVSKELTDM 
      KQLRKKLYN ARINKKLYD FEKIKIVHC VRTILETFT 
      DKMDPNFDC FVNDIKNWH KNEFFAHVR TLSTLKKLL 
      NRYRSDLKR FEDEIQARS DDNLKTIST LGLNKEKLF 
      DIVEKYKTN NLDIKTLVE ENLKLVEQM NELVVTLNS 
      NLSTDL
    CDS sequence
    • >TC009797 RA
      atgtccgtc ggtttgagt gaatcccag aactacatc 
      tggagcgac gatgattgt cttggagaa ggcactttc 
      ggtaaagtt tttcgtggc atacaaaaa cgatcgggt 
      gataaagtc gcaataaaa attttcaaa catgttaac 
      catcaagta tcacaagaa accaaatta ttgcaatca 
      ttacaacac aaacatatc atccgattt ttcgccacc 
      gaattcgag ctctccacc aataaaatg gtcatgatt 
      ttggaatta tgcaacggc ggaagcctc atgagctac 
      ttaaacgaa cccgaaaac gtttgtgga ttacccgat 
      gaagaattt ctactcgtt ttcaatcac ttatcagcg 
      gggttggag tttctgaag aggcacgat gtaattcac 
      cgtgacgtc aaaccagat aatataatg cgtcatgtg 
      cttgacgac ggtcaaacg gtttacaaa ctcgccgat 
      ttcggcaca gctcgggaa ctccccgag ggccaaaac 
      ttcacttcc ctccacggc accgagccc taccttcac 
      ccccaacta tacagggtg tgctttttc caggggagc 
      aactgcacg cgtgagttt ggggcgaac acggacctt 
      tggtcgatt ggtgtcacg ctttatcag acagcaacc 
      gggaattta ccctttctg gtgatggat cgccacgtg 
      atgcttcga atttaccac gaaatgcag gaaaatgaa 
      gggatcgtt tcggccatt caacagaaa aattccgac 
      gaagttgtc ttccagaaa aaattaccg cggaattgc 
      cgcttgagt tgcgggctc aaacagttg ataacgccc 
      ataattgcg ggggttttt atacaggaa aagggcaag 
      cagtggtcg tttacgcaa tattacgag caagtggtt 
      gacttagtt tcaaggaaa gtaattaac gttttttat 
      gttaataag atgcggctg atgaaggtg tacataaaa 
      tcggatgaa acgttcaac acttttaaa tattatgtt 
      tcggcacag agtgaggta gctgaagaa aatcaggtt 
      tttctttat aaggctaac ctgattgtg aacactttt 
      gaggatttt tccggtgtt gacgaatcg gattgtatt 
      tttttgttt gatcggagt aatgcagag gttgagtct 
      acaatcgat gttcagtta gtttttgaa aatcttacg 
      aattttcct aataagttt atgtcaagt agcgaatct 
      gatgatcat agacttgcc ttcgtgacg tgtcgggac 
      ttgtgtctt tgtaaaaat gtaatggat tatcagtcc 
      attgtttac aagtatttt agtaatttt atcaaggat 
      tttaatagt tactgtaaa gaggaaaca agagatcaa 
      cggaaaatg tacgacacg ttgttagag aaatcttca 
      cagactaaa aatcgggct cagatttta aaacaatta 
      caaaatttg aatcactcc aatgacgat tcattcctc 
      gataggttt gaagagatt agcgataat tttctttct 
      gaaacggac aaattgttc aacgatttg agaaatatt 
      aaagcggat ttagttcca aacacgtca cacgattct 
      tatctgaaa acggtgtct aaagaactt accgatatg 
      aagcagttg agaaaaaaa ttatataat gcacgaatt 
      aataaaaaa ttatacgat tttgaaaaa attaaaatt 
      gttcattgt gtgagaaca atattggaa acttttacc 
      gataaaatg gacccgaat tttgattgt tttgttaat 
      gatataaaa aattggcac aaaaatgaa ttttttgct 
      catgtgcgg actttaagt actttaaaa aagctactt 
      aatcgttac agaagcgat ttgaaacga tttgaggac 
      gaaattcag gcccgtagt gacgacaat ttgaaaact 
      attagcact ttgggactc aataaagaa aaattgttt 
      gatattgtt gaaaaatac aaaactaat aatttagat 
      attaagaca cttgttgag gaaaattta aaattggtt 
      gaacaaatg aatgaactg gttgtgacg ttgaactcg 
      aatttatcg actgattta taa
    mRNA sequence
    • >TC009797 RA
      tattttatt tatttattt ttgtttttg tgtgtgcca 
      gtgttttac tttgaaatt ttttttttg ggctgtatt 
      ttgttatgg tgtttttta atggcaaca ttggtataa 
      tcccatctg acatgtccg tcggtttga gtgaatccc 
      agaactaca tctggagcg acgatgatt gtcttggag 
      aaggcactt tcggtaaag tttttcgtg gcatacaaa 
      aacgatcgg gtgataaag tcgcaataa aaattttca 
      aacatgtta accatcaag tatcacaag aaaccaaat 
      tattgcaat cattacaac acaaacata tcatccgat 
      ttttcgcca ccgaattcg agctctcca ccaataaaa 
      tggtcatga ttttggaat tatgcaacg gcggaagcc 
      tcatgagct acttaaacg aacccgaaa acgtttgtg 
      gattacccg atgaagaat ttctactcg ttttcaatc 
      acttatcag cggggttgg agtttctga agaggcacg 
      atgtaattc accgtgacg tcaaaccag ataatataa 
      tgcgtcatg tgcttgacg acggtcaaa cggtttaca 
      aactcgccg atttcggca cagctcggg aactccccg 
      agggccaaa acttcactt ccctccacg gcaccgagc 
      cctaccttc acccccaac tatacaggg tgtgctttt 
      tccagggga gcaactgca cgcgtgagt ttggggcga 
      acacggacc tttggtcga ttggtgtca cgctttatc 
      agacagcaa ccgggaatt taccctttc tggtgatgg 
      atcgccacg tgatgcttc gaatttacc acgaaatgc 
      aggaaaatg aagggatcg tttcggcca ttcaacaga 
      aaaattccg acgaagttg tcttccaga aaaaattac 
      cgcggaatt gccgcttga gttgcgggc tcaaacagt 
      tgataacgc ccataattg cgggggttt ttatacagg 
      aaaagggca agcagtggt cgtttacgc aatattacg 
      agcaagtgg ttgacttag tttcaagga aagtaatta 
      acgtttttt atgttaata agatgcggc tgatgaagg 
      tgtacataa aatcggatg aaacgttca acactttta 
      aatattatg tttcggcac agagtgagg tagctgaag 
      aaaatcagg tttttcttt ataaggcta acctgattg 
      tgaacactt ttgaggatt tttccggtg ttgacgaat 
      cggattgta tttttttgt ttgatcgga gtaatgcag 
      aggttgagt ctacaatcg atgttcagt tagtttttg 
      aaaatctta cgaattttc ctaataagt ttatgtcaa 
      gtagcgaat ctgatgatc atagacttg ccttcgtga 
      cgtgtcggg acttgtgtc tttgtaaaa atgtaatgg 
      attatcagt ccattgttt acaagtatt ttagtaatt 
      ttatcaagg attttaata gttactgta aagaggaaa 
      caagagatc aacggaaaa tgtacgaca cgttgttag 
      agaaatctt cacagacta aaaatcggg ctcagattt 
      taaaacaat tacaaaatt tgaatcact ccaatgacg 
      attcattcc tcgataggt ttgaagaga ttagcgata 
      attttcttt ctgaaacgg acaaattgt tcaacgatt 
      tgagaaata ttaaagcgg atttagttc caaacacgt 
      cacacgatt cttatctga aaacggtgt ctaaagaac 
      ttaccgata tgaagcagt tgagaaaaa aattatata 
      atgcacgaa ttaataaaa aattatacg attttgaaa 
      aaattaaaa ttgttcatt gtgtgagaa caatattgg 
      aaactttta ccgataaaa tggacccga attttgatt 
      gttttgtta atgatataa aaaattggc acaaaaatg 
      aattttttg ctcatgtgc ggactttaa gtactttaa 
      aaaagctac ttaatcgtt acagaagcg atttgaaac 
      gatttgagg acgaaattc aggcccgta gtgacgaca 
      atttgaaaa ctattagca ctttgggac tcaataaag 
      aaaaattgt ttgatattg ttgaaaaat acaaaacta 
      ataatttag atattaaga cacttgttg aggaaaatt 
      taaaattgg ttgaacaaa tgaatgaac tggttgtga 
      cgttgaact cgaatttat cgactgatt tataacgtc 
      ttgttaatt ttgtaatta gttaagtat caaaatgtg 
      atgttttgc ataataaac atttattct gttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY